Abstract:
:4- to 8-cell mouse PIE's (preimplantation embryos) were cultured in vitro for 48 hr in media containing various concentrations of the anti-estrogen CI-628. Graded effects of the various concentrations were observed with the highest concentration (1.5 microng/ml) being 100% effective in blocking development to the blastocyst stage. The deleterious effects of the drug were prevented to some extent by including estradiol-17beta in the medium. Our interpretation is that the effect of CI-628 was due to its anti-estrogenic properties. If this interpretation is correct, then the results support our hypothesis that the development of mouse PIE's depends upon estrogen which originates in the PIE. :The hypothesis that mouse preimplantation embryos (PIEs) can synthesize estrogens and that PIE estrogen is a controlling factor during preimplantation embryogenesis was tested. Pregnant female mice of the Carworth strain (CFW1) were used. They were killed on the morning of either Day 3 or 4 of pregnancy. Mouse PIEs of 4-8 cells were exposed to an antiestrogen, CI-628, for 48 hours in media containing several concentrations of the antiestrogen. The concentration of 1.5 mcg/ml, the highest used, was 100% effective in blocking development of the blastocyst stage. The deleterious effects of the CI-628 were partly prevented by including estradiol-17beta in the medium. PIEs which did not develop appeared degenerating. CI-628, previously designated CN-55,945-27, is a nonsteroidal antiestrogen which acts by displacing estrogen from estrogen receptor sites. Its effect was thought to be due to its antiestrogenic properties. If so, results support the hypothesis that the development of mouse PIEs depends on estrogen which originates in the PIEs.
journal_name
Steroidsjournal_title
Steroidsauthors
Gupta JS,Dey SK,Dickmann Zdoi
10.1016/0039-128x(77)90005-8subject
Has Abstractpub_date
1977-03-01 00:00:00pages
363-9issue
3eissn
0039-128Xissn
1878-5867pii
0039-128X(77)90005-8journal_volume
29pub_type
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