Abstract：
INTRODUCTION:Pathogenesis of idiopathic pulmonary arterial hypertension (iPAH) includes endothelial dysfunction and in situ thrombosis. A hypercoagulable state has also been postulated but never demonstrated. Our objective was to determine whether patients with iPAH had a hypercoagulable state using calibrated automated thrombography (CAT), a new tool to phenotype coagulation in vitro. PATIENTS AND METHODS:16 patients with iPAH and 29 controls were studied. In vitro platelet dependent coagulation phenotyping by CAT monitored the activity of thrombin generation over time. Plasma levels of soluble thrombomodulin, tissue factor pathway inhibitor (TFPI) and von Willebrand factor (VWF) were measured as endothelial biomarkers. RESULTS:Endogenous thrombin potential (ETP) in the absence of activated protein C (APC) tended to be increased in patients compared to controls (1769 versus 1656 nM.min; p=0.053). ETP was higher in the presence of APC 25 nM (ETP-APC) in patients (781 versus 494 nM.min; p=0.005). Five patients had ETP-APC higher than the 95th centile of controls. Other CAT parameters (lag time, peak thrombin and time to peak) were all consistent with some degree of hypercoagulability in patients. Regarding endothelial plasma biomarkers sTM was lower (28.4 versus 40.6 μg/l, p=0.0108) in patients; TFPI antigen and activity (respectively: 14.3 versus 10.5 μg/l, p=0.0167; 1.155 versus 1.070, p=0.0021) and VWF (1300 versus 976%, p=0.0108) were higher in patients. CONCLUSION:We have demonstrated that at least some patients with iPAH have a hypercoagulable phenotype.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Tournier A,Wahl D,Chaouat A,Max JP,Regnault V,Lecompte T,Chabot Fdoi
10.1016/j.thromres.2010.08.020subject
Has Abstractpub_date
2010-12-01 00:00:00pages
e418-22issue
6eissn
0049-3848issn
1879-2472pii
S0049-3848(10)00465-2journal_volume
126pub_type
杂志文章abstract：:Clot dissolution using enzymes from the human fibrinolytic system has long been a goal of physicians and scientists. While recent focus has been on activators of the fibrinolytic system, new data support the use of plasmin itself. This supplement reviews the role of plasmin in fibrinolysis and locally administered pla...
journal_title：Thrombosis research
pub_type：
doi：10.1016/j.thromres.2008.06.008
更新日期：2008-01-01 00:00:00
abstract：:Because of a thrombasthenia-like platelet aggregation defect, platelets from dogs affected with Basset Hound Hereditary Thrombopathy were compared to normal control dog platelets by three different techniques in order to assess platelet membrane glycoprotein content. Crossed immunoelectrophoresis (CIE), two-dimensiona...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(86)90295-1
更新日期：1986-04-15 00:00:00
abstract：:A new approach to the investigation of accumulation of acridine derivatives and release by platelets is proposed. At high concentrations of acridine orange (AO) inside dense bodies, a two component fluorescence spectrum with maxima at 530 and 640 nm has been recorded. Computer deconvolution of spectra provided additio...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(87)90102-2
更新日期：1987-09-15 00:00:00
abstract：INTRODUCTION:We recently proposed a scale for assessment of patient-relevant functional limitations following an episode of venous thromboembolism (VTE). Further development of this post-VTE functional status (PVFS) scale is still needed. METHODS:Guided by the input of VTE experts and patients, we refined the PVFS sca...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2020.03.020
更新日期：2020-06-01 00:00:00
abstract：:Bleeding is an important complication of heparin therapy. A number of low molecular weight heparin fractions produce less bleeding than standard heparin for an equivalent antithrombotic effect in experimental animals. Low molecular weight heparin fractions and fragments are produced by a number of different procedures...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(84)90265-2
更新日期：1984-09-15 00:00:00
abstract：:Owing to the beneficial health effects on human cardiovascular system, soybeans and soy-related products have been a focus of intensive research. Soy isoflavones are known to be primarily responsible for the soy-related biological effects including anti-platelet activity but its in vivo relevancy has not been fully ve...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2013.01.002
更新日期：2013-03-01 00:00:00
abstract：:125I-fibronectin is incorporated into fibrin when blood plasma is clotted with thrombin. This process is dependent upon fibronectin concentration, temperature, and the presence of calcium ions. Fibronectin appears to be a very strong inhibitor of fibrin formation and influences both the thrombin clotting time and fibr...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(82)90308-5
更新日期：1982-09-01 00:00:00
abstract：:Microheterogeneity of antithrombin III (ATIII) was examined in pooled normal human plasma and serum by IEF/PA and immunoblotting techniques. A pattern consisting of 8 bands varying in isoelectric point from 4.7 to 5.2 was observed in citrated plasma following IEF in a pH range of 4-6. A similar pattern was observed in...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(85)90331-7
更新日期：1985-10-15 00:00:00
abstract：:The reunification of Germany in 1990 brought with it major challenges in terms of unifying the care offered to haemophilia patients. At that time, most of the treatment centres belonged to the largest regional hospitals. The centre for paediatric haemophilia patients in Leipzig was at the University Hospital. In this ...
journal_title：Thrombosis research
pub_type： 历史文章,杂志文章,评审
doi：10.1016/j.thromres.2013.10.018
更新日期：2014-11-01 00:00:00
abstract：:The use of platinum based chemotherapy in ovarian malignancy and other cancer types is known to be associated with deep vein thrombosis. In a prospective study of 47 patients with ovarian cancer of International Federation of Gynecology and Obstetrics stage Ib-IV, serial rheological parameters were determined (plasma ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(98)00022-x
更新日期：1998-04-15 00:00:00
abstract：:Bovine hide collagen dispersions were swollen in the pH range 1.6-7.0, treated with glutaraldehyde, and dialyzed to neutral pH. The intensity with which these collagens reacted with human platelets in plasma was studied by aggregometry and scanning electron microscopy. Collagen swollen at a pH below 4.25 +/- 0.30 and ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(89)90463-5
更新日期：1989-07-01 00:00:00
abstract：:Lysophosphatidic acid (LPA) is a lipid mediator generated by activated platelets and having various effects on numerous cell types. We investigated some effects of 1-oleyl LPA on vascular smooth muscle cells cultured from adult human normal arteries. At micromolar concentrations, LPA induced a mitogenic effect ([3H]-t...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(99)00004-3
更新日期：1999-06-01 00:00:00
abstract：:Systemic autoimmune diseases are characterised by the development of a relatively narrow spectrum of autoantibodies. These are of considerable diagnostic value. In addition, in some diseases, including anti-phospholipid syndrome, these autoantibodies can be directly pathogenic. Understanding how these autoantibodies a...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2004.06.037
更新日期：2004-01-01 00:00:00
abstract：:Venous thromboembolism (VTE) is a common complication in patients with malignant disease. In addition to well-established acquired risk factors for VTE, several genetic risk factors, mainly related to the haemostatic system, are known to influence thrombotic risk. However, the contribution of gene abnormalities to thr...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(03)00179-8
更新日期：2003-02-15 00:00:00
abstract：INTRODUCTION:Monocyte- and microparticle (MP)-associated tissue factor (TF) is upregulated in diabetes. Lipopolysaccharide (LPS) induces expression of TF and alternatively spliced TF (asTF) and increases MP release from monocytes. Using LPS-stimulated TF-bearing human monocytes, we examined whether glibenclamide, a sul...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2011.07.007
更新日期：2011-11-01 00:00:00
abstract：:The time course and degree of collagen induced platelet shape change, aggregation and ATP-secretion in citrated human platelet rich plasma was monitored with a lumi-aggregometer. Onset of ATP-secretion was measurable within seconds after collagen challenge and always distinctly before onset of shape change. In absence...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(86)90089-7
更新日期：1986-08-15 00:00:00
abstract：:The need for anticoagulation in dialysis patients is common and the incidence of venous thromboembolism (VTE) and atrial fibrillation in this population is high. While direct data are lacking on the management of anticoagulation in dialysis patients, careful weighing of risks and benefits on the basis of evidence from...
journal_title：Thrombosis research
pub_type： 杂志文章,实务指引
doi：10.1016/j.thromres.2005.03.031
更新日期：2006-01-01 00:00:00
abstract：:Highly purified bovine von Willebrand protein (vWP) has been studied with two complementary techniques. Light scattering measurements on different samples give weight average molecular weights in the range from 7 to 13 million, radius of gyration between 75 and 140 nm and diffusion coefficient near 4.15 X 10(-8) cm2/s...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(85)90289-0
更新日期：1985-11-15 00:00:00
abstract：:Lp(a) lipoprotein contains a unique apolipoprotein, apolipoprotein (a), that has a striking homology with plasminogen. This homology has brought forward speculations as to an inhibitory effect of Lp(a) lipoproteins on fibrinolysis. The present investigation was undertaken to study the influence of Lp(a) lipoprotein on...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(92)90080-t
更新日期：1992-11-01 00:00:00
abstract：:We investigated the alteration of heparan sulfate proteoglycans induced by thrombin in cultured vascular endothelial cells. Heparan sulfate proteoglycans, which were metabolically labeled with [3H] glucosamine and [35S] sulfate, were isolated by DEAE-Sephacel ion-exchange chromatography and characterized by molecular ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(97)00257-0
更新日期：1997-11-01 00:00:00
abstract：:The influence of acute hypoxemia on blood fibrinolytic activity was investigated in 12 healthy males. Physiologically significant hypoxemia was produced by inspiration of 13% oxygen for 30 min. Six healthy males were exposed to hypoxemia at rest and 6 males to hypoxemia during exhaustive physical exercise on an ergocy...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(87)90222-2
更新日期：1987-02-15 00:00:00
abstract：:Isolated rat liver macrophages (Kupffer cells) produce a factor(s) which stimulates fibrinogen synthesis 5-8 fold in hepatocyte cultures. The production of the stimulatory factor reached maximum during the first 24 hours the cells were in culture. When the cell culture medium was chromatographed on a calibrated Sephad...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(83)90025-7
更新日期：1983-10-15 00:00:00
abstract：:Stimulation of rat platelets with U46619 induced Ca2+ mobilization and platelet shape change, but aggregation and secretion were induced when platelets were stimulated with U46619 plus phorbol 12-myristate 13-acetate (PMA). However, stimulation of rabbit platelets with U46619 induced all the three platelet responses. ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(89)90171-0
更新日期：1989-10-15 00:00:00
abstract：:Incubation of recombinant human tissue factor apoprotein (Apo-TF) with human plasma decreased the recalcified clotting time of this plasma in a time-and dose-dependent manner suggesting relipidation of the Apo-TF by plasma lipoproteins. Incubation of Apo-TF with purified preparations of human very low density, low den...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(90)90182-c
更新日期：1990-11-01 00:00:00
abstract：:Bovine myelin basic protein (BP) induced a shape change and endogenous phosphorylation of a 45,000 (45K) molecular weight protein of intact human platelets. This effect occurred rapidly over an effective concentration range of 5-100 microM BP. BP peptides encompassing residues 1-42, 43-88, and 89-169 from the BP molec...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(82)90090-1
更新日期：1982-03-15 00:00:00
abstract：PURPOSE:The right ventricular outflow tract (RVOT) velocity time integral (VTI), an echocardiographic measure of stroke distance, correlates with cardiac index. We sought to determine the prognostic significance of low RVOT VTI on clinical outcomes among patients with acute pulmonary embolism (PE). MATERIALS AND METHO...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2020.06.049
更新日期：2020-11-01 00:00:00
abstract：:Ca2+ influx and efflux in unstimulated platelets and Ca2+ uptake by simultaneously isolated two membrane fractions of platelets from patients with myeloproliferative disorders (MPD) have been investigated. In MPD, Ca2+ influx and efflux across the plasma membrane in unstimulated platelets were in equilibrium at signif...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(89)90372-1
更新日期：1989-01-15 00:00:00
abstract：:An in vitro test system was developed to examine the effects of red blood cells (RBC) on shear-induced platelet adhesion (SIPAD) and platelet aggregation (SIPAG). Suspensions of human platelets labeled with Mepacrine and suspended in citrated plasma were exposed to single, continuous or repetitive (120-300x) one secon...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(98)00160-1
更新日期：1998-12-15 00:00:00
abstract：INTRODUCTION:Different Klebsiella pneumoniae strains carry different virulence factors and antibiotic resistance and may cause thrombocytopenia. This study aimed to investigate the effects of different infections caused by K. pneumoniae on platelets. METHODS:Two hypermucoviscous K. pneumoniae strains and two classic s...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2018.09.053
更新日期：2018-11-01 00:00:00
abstract：:Many patients with diabetes mellitus show increased platelet aggregation and prostaglandin synthesis in response to physiological agents such as ADP and collagen when their platelets are tested in platelet-rich plasma or washed platelet suspensions. However, the relationship between increased platelet aggregation in v...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(87)90265-9
更新日期：1987-06-01 00:00:00
