Abstract：
:The time course and degree of collagen induced platelet shape change, aggregation and ATP-secretion in citrated human platelet rich plasma was monitored with a lumi-aggregometer. Onset of ATP-secretion was measurable within seconds after collagen challenge and always distinctly before onset of shape change. In absence of platelet aggregation, the degree of collagen stimulation was correlated to onset of secretion, the secretion rate and onset of subsequent shape change. It is concluded that the secretion originated from the collagen-adherent platelets. Oral aspirin [1000 mg] did neither delay onset of secretion nor reduce the final amounts of ATP indicating that formation of TxA2 is not a necessary prerequisite for dense granule secretion during the primary activation process. EDTA and EGTA decreased the initial secretion rate, but may have interfered with the adhesion process. Micromolar concentrations of verapamil and yohimbine also reduced secretion, while the effect of theophylline was 100 times weaker. The results suggest that the primary activation process is vulnerable at the alpha-receptor-level and that the initial ATP-secretion requires Ca2+-entry rather than intra-platelet Ca2+-mobilization.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Malmgren Rdoi
10.1016/0049-3848(86)90089-7subject
Has Abstractpub_date
1986-08-15 00:00:00pages
445-53issue
4eissn
0049-3848issn
1879-2472journal_volume
43pub_type
杂志文章abstract：:This study evaluates the role of neutrophil granulocytes in mediating acute phase stimulation of fibrinogen synthesis. Turpentine was administered to neutropenic and non-neutropenic rats and fibrinogen synthetic rates measured in an isolated liver perfusion system. Using the (14C) carbonate technique for the measureme...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(82)90032-9
更新日期：1982-10-01 00:00:00
abstract：:Thrombus formation was electrically induced in the left common carotid artery of rats with preoccluded both vertebral and right carotid arteries. Following thrombus induction, animals became unresponsive and lost their righting reflex. The loss of righting reflex was observed when cortical tissular pO2 reached 55 +/- ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(87)90340-9
更新日期：1987-10-01 00:00:00
abstract：:The procoagulant cellular activity (PCA) of leukemic cells was evaluated, before and after endotoxin stimulation, in 38 patients with acute leukemia at presentation subdivided according to the FAB classification. In the M4 and M5 subgroups the stimulated leukemic cells showed a significant increase in the production o...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(87)90317-3
更新日期：1987-03-01 00:00:00
abstract：:To examine whether moderate hyperhomocyst(e)inemia is associated with coronary artery disease and the number of diseased coronary vessels in Koreans, we investigated 201 patients with angiographically documented coronary artery disease and 187 healthy subjects without evidence of stroke and coronary artery disease. Th...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(98)00197-2
更新日期：1999-04-01 00:00:00
abstract：:The influence of acute hypoxemia on blood fibrinolytic activity was investigated in 12 healthy males. Physiologically significant hypoxemia was produced by inspiration of 13% oxygen for 30 min. Six healthy males were exposed to hypoxemia at rest and 6 males to hypoxemia during exhaustive physical exercise on an ergocy...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(87)90222-2
更新日期：1987-02-15 00:00:00
abstract：:Tissue factor (TF) is an integral membrane glycoprotein that serves as a cofactor for blood coagulation factor VIIa. The induction of TF on the surface of endothelial cells is initiated by various kinds of stimuli including lipopolysaccharide (LPS), interleukin-1 beta (IL-1 beta), and tumor necrosis factor alpha (TNF ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(94)90002-7
更新日期：1994-04-15 00:00:00
abstract：:To explore social aspects of asymptomatic carriership of factor V Leiden mutation (FVL) and how carriers have experienced procedure of screening for FVL, we have performed a qualitative study using semi-structured interviews. Seventeen carriers of FVL without history of venous thromboembolism (VTE) were interviewed. C...
journal_title：Thrombosis research
pub_type： 临床试验,杂志文章
doi：10.1016/j.thromres.2004.02.002
更新日期：2004-01-01 00:00:00
abstract：:DMP 728 is a potent and specific platelet GPIIb/IIIa antagonist. Like all GPIIb/IIIa antagonists, DMP 728 has a steep dose-response relationship in inhibiting platelet aggregation. In this study the relationships between receptor occupancy, platelet aggregation and bleeding time was determined in anesthetized dogs aft...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(95)00029-1
更新日期：1995-03-15 00:00:00
abstract：:When human citrated plasma is dialysed against a phosphate buffer containing Ca++, citrate anions are removed, thrombin is generated and soluble fibrinogen derivatives (fibrin monomers and/or soluble fibrin polymers) are formed. These derivatives are able to combine with human or bovine elastin to form a very stable a...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(86)90061-7
更新日期：1986-07-15 00:00:00
abstract：INTRODUCTION:Even though thrombocytopenia following transcatheter aortic valve implantation (TAVI) has been described, further investigation of this phenomenon is needed. AIMS:To determine which factors may explain the fall in platelet count that occurs after implantation of a TAVI device, including markers of platele...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2017.05.020
更新日期：2017-08-01 00:00:00
abstract：:Defibrotide (D) is a natural polydeoxyribonucleotide from mammalian lungs with profibrinolytic and antithrombotic activities. D also has PGI2-stimulating and tissue plasminogen activator (TPA)-releasing activities, but has no anticoagulant properties. The protective effects of D were demonstrated very recently in a mo...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(86)90265-3
更新日期：1986-05-01 00:00:00
abstract：:Ajoene, the major antiplatelet compound derived from garlic, synergistically potentiates the antiaggregatory action of prostacyclin, forskolin, indomethacin and dypiridamole. For collagen-induced platelet aggregation in human PRP, the ID50 for ajoene is 95 +/- 5 microM. However, in the presence of the antiaggregatory ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(86)90259-8
更新日期：1986-05-01 00:00:00
abstract：:Coronary artery disease (CAD) continues to be the most frequent cause of death among women in the United States. Although elevated levels of clotting factors have been associated with CAD, few of these studies have been performed in women. Elevated levels of Factor XI have previously been associated with venous thromb...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(02)00190-1
更新日期：2002-07-15 00:00:00
abstract：:Reliable information on what patients with venous thromboembolism (VTE) are at increased risk to die of pulmonary embolism (PE) or bleeding (and the time course of the two fatal events) could likely help to better use anticoagulant therapy by improving selection of patients in whom its benefit will likely outweigh the...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/S0049-3848(19)30358-5
更新日期：2019-09-01 00:00:00
abstract：:The multiligand receptor, low density lipoprotein receptor-related protein (LRP), is implicated in processes such as atherosclerosis and fibrinolysis through its mediation of the catabolism of lipoproteins, proteases, and protease inhibitor complexes. The hepatoma cell line Hep G2 expresses LRP and has been used widel...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(97)00287-9
更新日期：1997-12-15 00:00:00
abstract：:The proteinase, brinase (Mr approximately 35000), from Aspergillus oryzae, which has been used in therapeutic attempts as a thrombolytic agent in arterial thrombosis, binds to purified human alpha 2-macroglobulin (alpha 2M) with a stoichiometry of 1.7-1.9 mol of enzyme/mol inhibitor. This binding leads to quantitative...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(88)90359-3
更新日期：1988-01-01 00:00:00
abstract：:Extended half-life (EHL) products have shown robust efficacy in clinical trials, whilst allowing for less intense treatment regimens when compared with standard half-life products. Regimen optimisation with EHL products could lead to further improvements in bleeding rates, quality of life and reductions in treatment b...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2019.12.012
更新日期：2020-12-01 00:00:00
abstract：:Increased fibrinolytic activity is a well recognized constant finding observed during cardiopulmonary bypass (CPB). The purpose of the present work was to study and estimate the factors involved in the plasminogen activation and prekallikrein-kallikrein systems in a population of adult patients undergoing open heart s...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(92)90018-6
更新日期：1992-09-01 00:00:00
abstract：:Amino sugars and basic amino acids inhibit platelet aggregation and the activity of the endogenous platelet lectin, yet, relatively high concentrations (approximately 30 mM) are required for the inhibition. If cooperative interactions are involved in these platelet surface activities, oligomers of primary amines shoul...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(84)90160-9
更新日期：1984-02-01 00:00:00
abstract：:We investigated on how to effectively administer orally factor VIII preparation to two patients with von Willebrand's disease as volunteers, and the following results were obtained: Bleeding time and aPTT were shortened, but no change of PT was observed. Factor VIII:C increased slightly, but no significant increase wa...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(85)90211-7
更新日期：1985-06-15 00:00:00
abstract：:The extrinsic fibrinolytic system was assessed among 124 consecutive survivors of acute myocardial infarction below 70 years of age. In samples drawn 3 months after discharge from hospital, the PAI-1 levels were higher and the tPA activities were lower than among elderly healthy controls. In contrast, the AMI survivor...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(87)90428-2
更新日期：1987-12-15 00:00:00
abstract：:Effects of vascular constriction on thrombotic occlusion was evaluated using rat mesenteric arteries and video-recording system attached to the microscope. Topical application of norepinephrine of 1, 10 and 100 micrograms/ml reduced the arterial diameter dose dependently from 297 +/- 41 mu to 166 +/- 50, 87 +/- 18 and...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(86)90178-7
更新日期：1986-10-01 00:00:00
abstract：INTRODUCTION:Although investigation for JAK2 V617F mutation is recommended in patients presenting with splanchnic venous thrombosis (SVT), no specific clinical advice is given to SVT patients presenting without myeloproliferative neoplasms (MPN) and JAK2 V617F mutation. In MPN-free SVT patients, to investigate the clin...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2013.07.014
更新日期：2013-08-01 00:00:00
abstract：OBJECTIVE:To explore the functional exercises of deep breathing and lower limb motions for the prevention of venous thrombosis in patients undergoing major surgery for gynecologic malignancy. METHODS:All eligible patients admitted to the gynecologic oncology unit were randomized into the study and control groups. A co...
journal_title：Thrombosis research
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.thromres.2019.04.013
更新日期：2019-06-01 00:00:00
abstract：:Urokinase-type plasminogen activator (uPA) plays a central role in many aspects of cellular regulation, such as fibrinolysis, cell migration, and metastasis. The uPA induction by inflammatory stimuli such as IL-1, TNFalpha, and lipopolysaccharide (LPS) has been reported in a number of human cells. We examined the effe...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(99)00174-7
更新日期：2000-03-01 00:00:00
abstract：:To investigate the relationships between tumor necrosis factor-alpha (TNF), interleukin-1 beta (IL-1 beta), soluble thrombomodulin (TM), and disseminated intravascular coagulation (DIC) in patients with systemic inflammatory response syndrome (SIRS), twenty-nine SIRS patients were classified into three groups; 4 patie...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(95)00207-3
更新日期：1995-12-15 00:00:00
abstract：:The extrinsic pathway of coagulation is initiated when zymogen factor VII binds to its cell surface receptor tissue factor. Recently recombinant human tissue factor has become available and therefore in this study the direct binding of human factor VII in plasma to recombinant tissue factor was explored. Factor VII bi...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(93)90103-u
更新日期：1993-05-15 00:00:00
abstract：BACKGROUND:Variability in platelet response to clopidogrel and its clinical relevance have been well described. However, the underlying mechanisms remain unclear. Recently, the T744C polymorphism of the P2Y12 receptor gene has been associated with enhanced platelet aggregation in healthy volunteers, suggesting a possib...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2007.01.012
更新日期：2007-01-01 00:00:00
abstract：INTRODUCTION:Congenital factor V (FV) deficiency is a rare inherited disorder. Three compound heterozygous missense mutations, Asp68His, His147Arg, and Arg2074Cys, were observed in a Taiwanese patient with moderately severe FV deficiency. METHOD:The novel His147Arg mutation in the A1 domain was investigated by protein...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2014.04.005
更新日期：2014-07-01 00:00:00
abstract：BACKGROUND:The plasminogen activator inhibitor type 1 (PAI-1) 4G/5G polymorphism may have significance for PAI-1 expression. High levels of PAI-1 in breast cancer patients are associated with a poor prognosis. In this study, we analyzed the influence of the PAI-1 4G/5G polymorphism on tissue PAI-1 levels and its associ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2005.03.025
更新日期：2006-01-01 00:00:00
