Abstract：
:The extrinsic pathway of coagulation is initiated when zymogen factor VII binds to its cell surface receptor tissue factor. Recently recombinant human tissue factor has become available and therefore in this study the direct binding of human factor VII in plasma to recombinant tissue factor was explored. Factor VII binding was quantitated by a standard ELISA protocol using monospecific polyclonal rabbit anti-human factor VII as the primary antibody and goat anti-rabbit IgG conjugated to alkaline phosphatase as the second antibody. Both the oxidation state of the recombinant tissue factor and calcium ion concentration were found to be critical for the efficient binding of factor VII. A linear relationship was observed between absorbance and factor VII concentration when normal pooled human plasma was diluted in the range 1:25 to 1:1000 (factor VII concentration 0.5-20 ng/ml). Evidence is provided to show that binding is both specific for human coagulation factor VII and can be utilized to detect factor VII molecular variants with impaired tissue factor binding.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Sridhara S,Clarke BJ,Ofosu FA,High KA,Blajchman MAdoi
10.1016/0049-3848(93)90103-usubject
Has Abstractpub_date
1993-05-15 00:00:00pages
307-16issue
4eissn
0049-3848issn
1879-2472pii
0049-3848(93)90103-Ujournal_volume
70pub_type
杂志文章abstract：BACKGROUND:Coagulopathy is a major cause of early death when arsenic trioxide (As(2)O(3)) therapy fails. In addition to the procoagulant properties of blast cells, the cytotoxic therapy may contribute to the coagulation disorders. The aim of the present study was to evaluate the possible impact of As(2)O(3) on membrane...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2011.01.003
更新日期：2011-05-01 00:00:00
abstract：:Biological effects of the modification of the sulphate ester and carboxyl group content of poly(vinyl alcohol-acrylic acid) copolymers (PAVAS) and sulphated polyvinyl alcohol copolymers (PVAS) with mol. weight of 5,000 to 20,000 D were studied. The in vitro anticoagulant potency of PAVAS assessed by activated partial ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(94)90154-6
更新日期：1994-05-15 00:00:00
abstract：INTRODUCTION:The Cilostazol Stroke Prevention Study found that cilostazol, a phosphodiesterase 3 inhibitor, can reduce the risk of subsequent stroke in Japanese patients with cerebral infarction. Here, we measured the effects of cilostazol in vitro on shear-induced platelet aggregation, an important mechanism of thromb...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2006.05.005
更新日期：2007-01-01 00:00:00
abstract：:The role of thrombin in vascular physiology and pathophysiology continues to impact our understanding of many cellular processes and systems including the function of platelets, endothelial cells, smooth muscle cells, leukocytes and the regulation of the coagulation cascade. Recent acute coronary syndrome clinical tri...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2015.02.013
更新日期：2015-05-01 00:00:00
abstract：:A platelet storage pool deficiency (SPD) is present in platelets from cattle with the Chediak-Higashi syndrome (CHS). The most plausible hypothesis for the SPD is that dense granule precursors are simply not formed in CHS megakaryocytes. There is, however, evidence that some recently acquired 5-hydroxytryptamine (5HT)...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(90)90096-u
更新日期：1990-05-01 00:00:00
abstract：:We have investigated fibrinolytic parameters in 33 patients with peripheral arterial occlusive disease (PAOD) before and 6, 24, and 48 hours after percutaneous transluminal angioplasty (PTA) as well as in 35 gender-matched healthy controls, whose mean age was not significantly different from the mean age of the patien...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(99)00002-x
更新日期：1999-05-15 00:00:00
abstract：:Treatment of deep venous thrombosis with low molecular weight heparin (LMWH-Novo, Logiparin) was carried out with two different doses of Logiparin, 75 XaI U/kg b.w. twice daily and 150 XaI U/kg b.w. once daily subcutaneously for 5 days. Simultaneously warfarin was given from the first day of heparin treatment. Mean ag...
journal_title：Thrombosis research
pub_type： 临床试验,杂志文章
doi：10.1016/0049-3848(89)90307-1
更新日期：1989-09-15 00:00:00
abstract：INTRODUCTION:After major liver surgery, there are risks of both postoperative bleeding and thrombosis. Routine coagulation monitoring is indicated, but may not provide adequate clinical guidance. Thus, we described the clotting status in a pilot study using broader coagulation testing. We analysed the temporal pattern ...
journal_title：Thrombosis research
pub_type： 临床试验,杂志文章
doi：10.1016/j.thromres.2015.05.023
更新日期：2015-08-01 00:00:00
abstract：:Ajoene, the major antiplatelet compound derived from garlic, synergistically potentiates the antiaggregatory action of prostacyclin, forskolin, indomethacin and dypiridamole. For collagen-induced platelet aggregation in human PRP, the ID50 for ajoene is 95 +/- 5 microM. However, in the presence of the antiaggregatory ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(86)90259-8
更新日期：1986-05-01 00:00:00
abstract：:Four calcium channel blockers (nimodipine, nifedipine, verapamil and diltiazem) of three chemical classes were tested in vitro for inhibition of platelet aggregation using heparinized human platelet rich plasma. Both ADP- and thrombin-induced aggregation were inhibited as was the biosynthesis of thromboxane A2 in resp...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(84)90241-x
更新日期：1984-06-01 00:00:00
abstract：:The diagnosis of preeclampsia, with all of its consequences, is at times difficult to establish, especially when the patient has underlying chronic hypertension and is not known from prior prenatal care visits. Many screening tests have been proposed. These should be sensitive, relatively specific, easy to perform, of...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(94)90065-5
更新日期：1994-07-15 00:00:00
abstract：:Generally, the rules of good practice in transfusion medicine apply also to the pediatric age group. However, the frequency of specific diseases that might necessitate the administration of fresh frozen plasma (FFP) differs from that in adults. Physiologic differences to the later age exist in the neonatal period and ...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/s0049-3848(02)00149-4
更新日期：2002-10-31 00:00:00
abstract：BACKGROUND AND AIM:Recent studies have documented that elevation of C-reactive protein (CRP) levels after percutaneous coronary intervention (PCI) have been predictive of adverse outcome. This study was performed to test the hypothesis that preprocedural use of naproxen sodium is associated with a reduction in the exte...
journal_title：Thrombosis research
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.thromres.2006.11.003
更新日期：2007-01-01 00:00:00
abstract：:The hemolytic uremic syndrome involves the presence of Shiga toxin producing strains of Escherichia coli and is associated with thrombocytopenia, platelet activation, and microthrombi formation. We have, therefore, investigated the ability of Shiga toxin isotypes 1 and 2 to cause or enhance platelet aggregation under ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(00)00191-2
更新日期：2000-06-01 00:00:00
abstract：:The present investigation has used an aggregation-disaggregation-reaggregation model to determine if exposure to potent aggregating agents causing a refractory state in deaggregated platelets results in down-regulation and clearance of GPIb/IX, the receptor for von willebrand factor. Thrombin, the thrombin receptor ac...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(96)00185-5
更新日期：1996-11-15 00:00:00
abstract：INTRODUCTION:Hydrogen sulfide (H2S) known as a gasotransmitter is increasingly recognized for its anti-adhesive, anti-inflammatory and vasoactive properties. Due to these properties, we analysed anti-thrombotic effects of H2S and the participation of the nitric oxide synthase (NOS)-pathway. MATERIALS AND METHODS:In in...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2013.07.010
更新日期：2013-08-01 00:00:00
abstract：:The influence of red blood cells on platelet aggregation has recently been a subject of considerable interest. We have studied the effect of red cells on spontaneously formed platelet aggregates in rotating vials at 37 degrees C. Platelet aggregation was quantified by measuring the fall in number of single platelets w...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(85)90150-1
更新日期：1985-05-01 00:00:00
abstract：:Human plasma Lp(a) is susceptible to various sulfhydryl compounds. In this study we present evidence indicating that after treatment of Lp(a) with sulfhydryl compounds, immunoreactivity is changed, structural changes occur and functional characteristics regarding the numerous kringle structures in apo(a) disappear. Pu...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(94)90110-4
更新日期：1994-05-01 00:00:00
abstract：INTRODUCTION:The mortality rate associated with UGI bleeding remains high at 7-14%. Pharmacologic and endoscopic interventions are the current standard treatment, but there are few alternative options should these fail. This study aimed to assess the efficacy and safety of recombinant activated factor VII (rFVIIa) in t...
journal_title：Thrombosis research
pub_type： 临床试验,杂志文章
doi：10.1016/j.thromres.2005.10.008
更新日期：2006-01-01 00:00:00
abstract：:Despite clear potential benefits of outpatient care, most patients suffering from pulmonary embolism (PE) are currently hospitalized due to the fear of possible adverse events. Nevertheless, some teams have increased or envisage to increase outpatient treatment or early discharge. We performed a narrative systematic r...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2017.05.001
更新日期：2017-07-01 00:00:00
abstract：:The ability of parenteral heparin to release the capillary-bound enzymes lipoprotein lipase (LPL) and hepatic triglyceride lipase (HTGL) into the circulation is shared by several other sulphated polysaccharides. The lipase-releasing activity in rats of two unfractionated heparins has been compared with that of charact...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(89)90068-6
更新日期：1989-08-01 00:00:00
abstract：:Platelets from rats fed a diet high in linoleic acid (6%) bound increased amounts of fibrinogen on stimulation with ADP, compared to those from rats fed diets with low (2%) or no linoleic acid. However, this increased fibrinogen binding was associated with a decrease in platelet aggregation induced by ADP. Changes in ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(84)90107-5
更新日期：1984-04-01 00:00:00
abstract：UNLABELLED:We undertook a survey of French university hospital hematological laboratories to ascertain the clinical characteristics of patients with suspected HIT, the laboratory tests performed, and the therapeutic strategy adopted in current practice. METHODS:A standardized medical records database for patients with...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2010.02.003
更新日期：2010-06-01 00:00:00
abstract：:The thrombolytic efficacy of recombinant tissue-type plasminogen activator (tPA) in the presence and absence of a thromboxane synthase inhibitor was studied in a model of femoral artery thrombosis in the anesthetized rabbit. The thrombus was formed by injection of thrombin and whole blood into an isolated segment of t...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(88)90022-9
更新日期：1988-12-01 00:00:00
abstract：:Cell based models of coagulation (CBM) have provided mechanistic insight into numerous hematological issues for nearly two decades. This review discusses another coagulation model system--the clot lifespan model (CLSM)--that has been designed to compliment the CBM-based approach to elucidating the mechanisms responsib...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2007.09.003
更新日期：2008-01-01 00:00:00
abstract：INTRODUCTION:Edoxaban is an oral, once-daily direct factor Xa (FXa) inhibitor. Although rapidly cleared, strategies to reverse edoxaban-mediated effects on anticoagulation are needed in cases of excessive bleeding or emergency. This study evaluated the effect of two prohemostatic agents, recombinant factor VIIa (rFVIIa...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2014.07.036
更新日期：2014-10-01 00:00:00
abstract：INTRODUCTION:Antiphospholipid syndrome (APS) is a pro-thrombotic autoimmune disease that affects different vascular beds, with potential risk for recurrence. Systemic lupus erythematosus (SLE), specific autoantibodies profile and atherogenic disorders have been described as risk factors for the occurrence of first thro...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2015.10.029
更新日期：2015-12-01 00:00:00
abstract：:In this study, we investigated the effects of a 3-min insulin incubation both at physiological and at supraphysiological concentrations on platelet aggregation and intraplatelet cyclic guanosine monophosphate (cGMP) levels both in the absence and in the presence of phosphodiesterase inhibition. We observed that insuli...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(96)00051-5
更新日期：1996-04-01 00:00:00
abstract：:Enzymatic treatment used for passaging of endothelial cells may induce release of von Willebrand factor (vWF). Decreased ability to replenish intracellular stores results in decreased secretion of vWF in later passages of cells. Since both trypsin and pancreatin complex have been used for passaging endothelial cells, ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(00)00401-1
更新日期：2001-02-15 00:00:00
abstract：:Two monoclonal antibodies against plasminogen and two against alpha-2-antiplasmin were characterized regarding their binding properties towards their respective native and modified antigens and towards the plasmin-alpha-2-antiplasmin complex. Using an ELISA system, the two monoclonal antibodies against plasminogen (MP...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(87)90311-2
更新日期：1987-03-01 00:00:00
