Abstract:
:In this study, we investigated the effects of a 3-min insulin incubation both at physiological and at supraphysiological concentrations on platelet aggregation and intraplatelet cyclic guanosine monophosphate (cGMP) levels both in the absence and in the presence of phosphodiesterase inhibition. We observed that insulin at concentration in the range 0.25-2 nmol/L decreases platelet response to adenosine 5-diphosphate (ADP), being Effective Dose 50 (ED50) for ADP with 2 nmol/L insulin 164 +/- 15% of the basal value, p = 0.005; furthermore, insulin increases intraplatelet content of cGMP (from basal 7.3 +/-0.6 pmol/10(9) plts to 14.6 +/- 1.2 pmol/10(9) plts with 2 nmol/L insulin, p=0.0001) and does not affect the platelet cGMP increase induced by nitrates. On the contrary, at very elevated concentrations (25-200 nmol/L) insulin increases platelet aggregation to ADP (ADP ED50 with 200 nmol/L insulin being 81 +/- 4% of the basal value, p = 0.01), decreases intraplatelet content of cGMP (from basal 7.2 +/- 0.1 pmol/10(9) plts to 5.7 +/- 0.2 pmol/10(9) plts with 200 nmol/L insulin, p = 0.01) and attenuates the platelet cGMP increase induced by nitrates. When cGMP catabolism is inhibited by theophylline or the selective cGMP phosphodiesterase inhibitor zaprinast, insulin shows anti-aggregating effects also at highly supraphysiological concentration (25-200 nmol/L). These results indicate that insulin, depending on the concentrations employed, shows opposite effects on platelet function, and they provide information about the mechanisms involved: actually, insulin is able to increase both cGMP synthesis, through guanylate cyclase activation, and cGMP catabolism, through phosphodiesterase activation. At physiological or slightly supraphysiological concentrations the first phenomenon is prevailing, so that cGMP intraplatelet values increase and insulin shows antiaggregating properties, whereas, at supraphysiological concentrations, insulin reduces cGMP levels through a prevailing phosphodiesterase activation, as supported by the fact that, when cGMP catabolism is prevented, insulin shows anti-aggregating properties also at the highest concentrations used.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Anfossi G,Massucco P,Mattiello L,Piretto V,Mularoni E,Cavalot F,Paoletti G,Trovati Mdoi
10.1016/0049-3848(96)00051-5subject
Has Abstractpub_date
1996-04-01 00:00:00pages
57-68issue
1eissn
0049-3848issn
1879-2472pii
0049384896000515journal_volume
82pub_type
杂志文章abstract::Platelets occupy a central role at the interface between thrombosis and inflammation. At sites of vascular damage, adherent platelets physically and functionally interact with circulating leukocytes. Activated platelets release soluble factors into circulation that may have local and systemic effects on blood and vasc...
journal_title:Thrombosis research
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abstract::Prothrombin time is a standard screening test to monitor patients treated with oral anticoagulants. We hypothesize that INR measurements will not vary a lot within a given day in a given patient. The present study was thus conducted to compare the change of the international normalized ratio (INR) of prothrombin time ...
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更新日期:2002-09-01 00:00:00
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更新日期:2019-02-01 00:00:00
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journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/j.thromres.2013.04.017
更新日期:2013-06-01 00:00:00
abstract::Platelet aggregation induced by ADP and collagen was monitored in 25 patients with increased serum levels of triglycerides (7 HLPs type IIb, 14 type IV, 4 type V patients) and compared with a group of 10 normolipidaemic control persons. Platelet aggregation was studied simultaneously in platelet rich plasma (PRP) by b...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(86)90238-0
更新日期:1986-02-01 00:00:00
abstract::Analogs of the antithrombin peptide hirudin54-65 with C-terminal modifications have been synthesized in order to examine the requirements for alpha-thrombin inhibition. The C-terminal residue, Gln65, could be replaced with L-amino acids or amino alcohols with neutral or charged hydrophilic side chains without greatly ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(89)90090-x
更新日期:1989-05-15 00:00:00
abstract:INTRODUCTION:The use of oral anticoagulant therapy is increasing in children. Managing anticoagulant therapy in children presents unique challenges, including poor venous access. The advent of point-of-care (POC) monitoring of anticoagulant therapy offers a potential solution to this challenge. This paper reviews the p...
journal_title:Thrombosis research
pub_type: 杂志文章,meta分析
doi:10.1016/j.thromres.2005.03.008
更新日期:2006-01-01 00:00:00
abstract::The influence of acute hypoxemia on blood fibrinolytic activity was investigated in 12 healthy males. Physiologically significant hypoxemia was produced by inspiration of 13% oxygen for 30 min. Six healthy males were exposed to hypoxemia at rest and 6 males to hypoxemia during exhaustive physical exercise on an ergocy...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(87)90222-2
更新日期:1987-02-15 00:00:00
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journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(91)90189-4
更新日期:1991-05-01 00:00:00
abstract::Heparin is an effective, relatively safe, inexpensive parenteral antithrombotic agent widely used in the prevention and treatment of thromboembolic disorders, but it has several limitations such as the marked intra- and inter-patient variability in its anticoagulant response, its poor bioavailability at low doses and ...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/j.thromres.2011.11.008
更新日期:2012-03-01 00:00:00
abstract::125I-fibronectin is incorporated into fibrin when blood plasma is clotted with thrombin. This process is dependent upon fibronectin concentration, temperature, and the presence of calcium ions. Fibronectin appears to be a very strong inhibitor of fibrin formation and influences both the thrombin clotting time and fibr...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(82)90308-5
更新日期:1982-09-01 00:00:00
abstract:BACKGROUND:Anticoagulated patients are often seen unnecessarily in the emergency department (ED) for epistaxis, leading to increased healthcare costs. Patients are often unaware of preventative and management techniques for handling epistaxis in the home. METHODS:In 2016, the Michigan Anticoagulation Quality Improveme...
journal_title:Thrombosis research
pub_type: 信件
doi:10.1016/j.thromres.2021.01.007
更新日期:2021-01-16 00:00:00
abstract::We have investigated fibrinolytic parameters in 33 patients with peripheral arterial occlusive disease (PAOD) before and 6, 24, and 48 hours after percutaneous transluminal angioplasty (PTA) as well as in 35 gender-matched healthy controls, whose mean age was not significantly different from the mean age of the patien...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(99)00002-x
更新日期:1999-05-15 00:00:00
abstract::Hypercoagulable states can be detected by measuring activation peptides, enzyme-inhibitor complexes, and fibrin/fibrinogen degradation products, which are markers of hemostatic activation. A series of these prethrombotic markers has been evaluated in the elderly, pregnancy, diabetes and acute myocardial infarction pat...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(98)00165-0
更新日期:1999-01-15 00:00:00
abstract::Factor Xa-catalyzed prothrombin activation occurs on cellular surfaces, including those of vascular endothelial cells. In a reconstituted model, endothelial cells and platelets acted synergistically to maximally activate prothrombin in the presence of Factor Xa. Synergism was observed at platelet concentrations less t...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(87)90168-x
更新日期:1987-01-15 00:00:00
abstract::Reliable information on what patients with venous thromboembolism (VTE) are at increased risk to die of pulmonary embolism (PE) or bleeding (and the time course of the two fatal events) could likely help to better use anticoagulant therapy by improving selection of patients in whom its benefit will likely outweigh the...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/S0049-3848(19)30358-5
更新日期:2019-09-01 00:00:00
abstract:BACKGROUND:The purpose of this study was to determine whether the administration of multiple boluses of oral and intravenous tranexamic acid (TXA) postoperatively was equivalent in reducing blood loss in primary THA. METHODS:A total of 108 patients were randomized into two groups: oral TXA group (54 patients receiving...
journal_title:Thrombosis research
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.thromres.2018.02.007
更新日期:2018-04-01 00:00:00
abstract:BACKGROUND:The methodology of thromboprophylaxis post minimally invasive esophagectomy (MIE) is unclear. Thus, we compared the efficacy and safety of fondaparinux and nadroparin on the prophylaxis of venous thromboembolism (VTE) after MIE. MATERIALS AND METHODS:We conducted a randomized, double-blind, treatment-contro...
journal_title:Thrombosis research
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.thromres.2018.04.001
更新日期:2018-06-01 00:00:00
abstract::Capsaicin was found to be a potent inhibitor of platelet aggregation and release reaction. It inhibited the aggregation of rat platelet induced by collagen and thrombin, but only slightly reduced those of AA and A23187. The IC50 on collagen-induced platelet aggregation was about 85 micrograms/ml. Less inhibition was o...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(84)90189-0
更新日期:1984-12-15 00:00:00
abstract::Hemorrhagic tendency is frequent in uremic patients and is often associated with prolonged bleeding time and decreased platelet functions in vitro. Similarly, in multiple myeloma, platelet functions are affected, mainly by the inhibitory effect of paraprotein. We have studied patients with renal insufficiency and mult...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(02)00003-8
更新日期:2002-02-15 00:00:00
abstract::Disadvantages of current bleeding time methods include poor reproducibility, limited repeatability, and at times scar formation. We report a new, in vitro technique which circumvents these problems. Venous blood is collected in sodium citrate, stored capped in a siliconized tube at 37 degrees C until use, and made to ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(83)90011-7
更新日期:1983-07-01 00:00:00
abstract::Heparin with low affinity (LA-heparin) to antithrombin III (AT III) enhanced the rate of inactivation of thrombin by AT III. The enhancement of the rate was saturable with AT III and was proportional to the LA-heparin concentration. Although the rate-enhancement in the presence of LA-heparin decreased with increase in...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(86)90247-1
更新日期:1986-02-01 00:00:00
abstract:BACKGROUND:Statins have been shown to have a protective effect for venous thromboembolism (VTE) in the general population. This study sought to assess the association between statins and the risk for cancer-associated deep vein thrombosis (DVT) and pulmonary embolism (PE). METHODS:Patients with newly diagnosed cancer ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2017.08.001
更新日期:2017-10-01 00:00:00
abstract::This study compares selected properties of purified tryptic fragments of human von Willebrand (vW) protein in order to better understand how the M(r) 116,000 fragment retains activity in ristocetin-induced platelet agglutination. Wheat germ agglutinin (WGA) aggregation of platelet-rich plasma was potentiated by preinc...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(83)90408-5
更新日期:1983-08-01 00:00:00
abstract::Whole blood electrical aggregometry (WBEA) has become an accepted method to gain quick information on platelet disorders. Compared to the optical method WBEA is closer to physiology and less complicated, but on the other hand more difficult to standardize. Different approaches have been attempted in the past to improv...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(90)90232-2
更新日期:1990-12-15 00:00:00
abstract:INTRODUCTION:Dermatan sulfate, a sulfated glycosaminoglycan, acts as an anticoagulant by accelerating the inhibition of thrombin by heparin cofactor II. MATERIALS AND METHODS:A low molecular mass dermatan sulfate was obtained by peroxy-radical depolymerization from a parent dermatan sulfate. Chemical characterization ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2007.09.001
更新日期:2008-01-01 00:00:00
abstract:INTRODUCTION:Pelvic deep vein thrombosis (DVT) is difficult to diagnose during pregnancy. In a two-center trial, we evaluated the agreement between ultrasonography and magnetic resonance imaging (MRI) in diagnosing the extent of DVT into the pelvic veins during pregnancy. MATERIALS AND METHODS:Pregnant women with prox...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2010.05.011
更新日期:2010-08-01 00:00:00
abstract::Heparanase is an endo-beta-D-glucuronidase capable of cleaving heparan sulfate (HS) side chains of heparan sulfate proteoglycans (HSPG) on cell surfaces and the extracellular matrix, activity that is strongly implicated in tumor metastasis and angiogenesis. Evidence was provided that heparanase over-expression in canc...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/S0049-3848(10)70018-9
更新日期:2010-04-01 00:00:00
abstract:BACKGROUND/AIM:It is believed that the amniotic fluid-derived TF, in the case of amniotic fluid embolism (AFE), contributes to acute disseminated intravascular coagulation (DIC) and obstetric shock in the mother. However, the role of amniotic fluid-derived contact phase coagulation factors that irrupt into the bloodstr...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2014.10.014
更新日期:2015-04-01 00:00:00
abstract::Vascular endothelial injury (VEI) triggers pathological processes in various cardiovascular diseases, such as coronary heart disease and hypertension. To further elucidate the in vivo pathological mechanisms of VEI, many animal models have been established. For the easiness of genetic manipulation and feeding, murine ...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/j.thromres.2018.07.014
更新日期:2018-09-01 00:00:00