Abstract:
:Disadvantages of current bleeding time methods include poor reproducibility, limited repeatability, and at times scar formation. We report a new, in vitro technique which circumvents these problems. Venous blood is collected in sodium citrate, stored capped in a siliconized tube at 37 degrees C until use, and made to flow under constant pressure through a filter of Woven Dacron. Flow rate progressively falls as platelet aggregates occlude the filter, as verified by scanning electron microscopy and 111Indium-labeled platelet radioactivity of the filter. Filter bleeding time (FBT) was 4.33 +/- 1.79 (means x +/- SD) min in 23 healthy human volunteers and 3.08 +/- 1.08 min in 14 normal dogs. Bleeding volume (BV) (number of drops) was 29 +/- 12 in the humans and 16 +/- 6 in the dogs. Initial bleeding rate (IBR) (number of drops during first minute) was 14 +/- 3 in citrated blood and 25 +/- 5 drops/min in EDTA blood of the humans (P = 9 X 10(-10)), 8 +/- 2 in citrated blood and 14 +/- 6 drops/min in EDTA blood of the dogs (P = .0004). Percent platelet reduction during passage of blood through the filter (PCR) was 27.5 +/- 7.2 in citrated blood and 4.9 +/- 2.6 in EDTA blood of the humans (P = 2 X 10(-12)), while in dogs it was 25.7 +/- 8.3 in citrated blood and 5.3 +/- 4.2 in EDTA blood (P = 8 X 10(-7)). The results of these parameters using human platelet rich plasmas (PRPs) were similar to those with human whole blood, but significant decrease of drop rate was not observed in canine PRPs during passage of PRP through the filter. FBT, BV, and IBR were correlated (r = -0.91, -0.84 and -0.62 respectively) with platelet count in 12 specimens obtained from 4 dogs in which thrombocytopenia (107 to 4 X 10(3) platelets/microliter) was induced by estradiol. This new test is unaffected by specimen transport by pneumatic tube, is sensitive to platelets, and should be useful for analysis of both quantitative and qualitative platelet abnormalities in man.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Uchiyama S,Stropp JQ,Claypool DA,Didisheim P,Dewanjee MKdoi
10.1016/0049-3848(83)90011-7subject
Has Abstractpub_date
1983-07-01 00:00:00pages
99-115issue
1eissn
0049-3848issn
1879-2472journal_volume
31pub_type
杂志文章abstract::The extracellular nucleotides, ATP and ADP, as well as adenosine have been implicated in a great number of physiological functions. ADP is one of the major platelet recruiting factors, whereas ATP is considered to be a competitive inhibitor of ADP-induced platelet aggregation and adenosine is able to induce vasodilata...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2007.01.006
更新日期:2007-01-01 00:00:00
abstract::The anticoagulant (U.S.P., APTT; "in vitro" and "in vivo") antithrombotic (aXa; Yin and Wessler and chromogenic), antilipemic (LPL) activities of heparin, heparin fractions and fragments, heparinoids, heparan sulfate and other sulfated glycosaminoglycans were compared with the activities of these compounds as antithro...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(85)90298-1
更新日期:1985-12-01 00:00:00
abstract:BACKGROUND:There is a substantial local release of tissue-type plasminogen activator (t-PA) in the splanchnic vascular bed, and this release is increased at high sympathetic tone. Coronary t-PA release is also significant, and this release increases during cardiac nerve stimulation and during reperfusion after 10 min o...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2009.10.004
更新日期:2010-03-01 00:00:00
abstract::Ethamsylate possesses antihemorrhagic properties, but whether or not it directly activates blood platelets is unclear. Here we investigated the platelet activation potential of ethamsylate, by measuring membrane P-selectin expression with flow cytometry in human whole blood and also by immunofluorescence imaging of is...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(02)00353-5
更新日期:2002-09-15 00:00:00
abstract::18F-Fluorodesoxyglucose Positron-Emission-Tomography combined with Computed-Tomography (FDG PET/CT) might be an attractive tool for cancer screening in patients with venous thromboembolism (VTE), allowing non-invasive whole-body imaging. One of the frequent criticisms to the use of FDG PET/CT for screening is the pote...
journal_title:Thrombosis research
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.thromres.2017.04.022
更新日期:2017-07-01 00:00:00
abstract::Selective serotonin reuptake inhibitors (SSRIs) have been reported to reduce platelet aggregation induced by ADP. ADP induces platelet aggregation through two purinergic receptors P2Y1 and P2Y12. We characterized the inhibitory properties of SSRIs on ADP-induced platelet aggregation and investigated the effects of SSR...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2013.02.007
更新日期:2013-04-01 00:00:00
abstract::Homogenous preparations of human and rabbit platelets synthesized platelet-activating factor (PAF) when stimulated by calcium ionophore A23187. PAF was isolated by high performance liquid chromatography (HPLC) on a Lichrosorb Si60 column. PAF was well separated from other phospholipids especially sphingomyelin and lys...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(83)90398-5
更新日期:1983-04-01 00:00:00
abstract::Full-length Tissue Factor (flTF) - the obligatory co-factor for the serine protease (factor) VII/VIIa - serves as the initiator of blood coagulation. The flTF/VIIa complex triggers a sequence of proteolytic events that lead to the formation of a hemostatic plug. Aside from hemostatic maintenance, flTF can contribute t...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/j.thromres.2012.02.027
更新日期:2012-05-01 00:00:00
abstract::An in vivo rabbit model of venous thrombosis which includes physiological blood flow was used to compare the efficacy of the potent and specific factor Xa inhibitor recombinant tick anticoagulant peptide (rTAP) with standard heparin in the prevention of venous thrombus formation. In anesthetized rabbits, an autologous...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(93)90200-8
更新日期:1993-08-15 00:00:00
abstract::The potency for the inhibition of 5-hydroxytryptamine (5-HT)-induced human platelet aggregation by ketanserin, pipamperone, spiperone, cyproheptadine and BW 501 in vitro decreased as the reaction pH increased progressively from 7.4 to 8.6, the largest shift (X 1125) in IC50 value (pH 7.4: 4 X 10(-9) M; pH 8.6: 4.5 X 1...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(85)90297-x
更新日期:1985-12-01 00:00:00
abstract:INTRODUCTION:Type 2 diabetes mellitus (T2DM) patients have a variable response profile to the P2Y(12) receptor antagonist clopidogrel. P2Y(12) receptor signalling promotes platelet procoagulant activity. The aim of this study was to determine if T2DM patients with suboptimal clopidogrel response have greater platelet p...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2008.10.001
更新日期:2009-07-01 00:00:00
abstract::The daily production of billions of platelets must be regulated to avoid spontaneous bleeding or arterial occlusion and organ damage. Complex mechanisms control platelet production and clearance in physiological and pathological conditions. This review will focus on the mechanisms of platelet senescence with specific ...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/S0049-3848(16)30370-X
更新日期:2016-05-01 00:00:00
abstract::The treatment of prostatic cancer with oestrogen has been reported to be associated with cardiovascular side effects. Twenty patients with recently diagnosed prostatic cancer were randomly allocated to oestrogen therapy or orchidectomy. As compared to healthy age matched controls the patients with prostatic cancer had...
journal_title:Thrombosis research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/0049-3848(86)90024-1
更新日期:1986-12-15 00:00:00
abstract::The specific thromboxane A2/prostaglandin H2 (TXA2/PGH2) antagonist 13-azaprostanoic acid (13-APA) reverses platelet aggregation stimulated by TXA2/PGH2 and the prostaglandin endoperoxide analog U46619. The present report demonstrates that the deaggregatory properties of 13-APA are potentiated by prostacyclin (PGI2). ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(82)90097-4
更新日期:1982-12-15 00:00:00
abstract::Human tissue plasminogen activator (t-PA) was shown to bind specifically to human osteosarcoma cells (HOS), and human epidermoid carcinoma cells (A-431 cells). Crosslinking studies with DTSSP demonstrated high molecular weight complexes (130,000) between 125I-t-PA and cell membrane protein on human umbilical vein endo...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(90)90136-z
更新日期:1990-07-15 00:00:00
abstract::The interaction of platelets with collagen involves short aminoacid sequences which recur along the fibres. Platelet aggregation by collagen and serotonin release is inhibited by a synthetic octapeptide LYS-PRO-GLY-GLU- PRO-GLY-PRO-LYS- derived from type III collagen. In contrast, this octapeptide inhibits only weakly...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(83)90061-0
更新日期:1983-12-15 00:00:00
abstract::Platelet aggregation, lipoprotein lipase activity, coagulation parameters and routine blood chemistry were measured in a randomised study of 21 surgical patients before, immediately after and 3 months after operation. Sodium heparin 5000 IU was given subcutaneously to 11 patients every 12 hours for 7 days, the first i...
journal_title:Thrombosis research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/0049-3848(83)90187-1
更新日期:1983-06-01 00:00:00
abstract::Lysophosphatidic acid (LPA) is a lipid mediator generated by activated platelets and having various effects on numerous cell types. We investigated some effects of 1-oleyl LPA on vascular smooth muscle cells cultured from adult human normal arteries. At micromolar concentrations, LPA induced a mitogenic effect ([3H]-t...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(99)00004-3
更新日期:1999-06-01 00:00:00
abstract::It has been found in our laboratory that compression induces disaggregation and decompression induces aggregation of human platelets. The experimental design was based on the premise that oppositely charged amino acid residues of fibrinogen are juxtaposed in the specific adhesive site(s). This can be explained by the ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(87)90083-1
更新日期:1987-03-15 00:00:00
abstract::A synthetic xanthone derivative, 3-[2-(cyclopropylamino)ethoxy] xanthone (CPEX), was investigated for its antiplatelet activities in washed rabbit platelets and human platelet-rich plasma. CPEX inhibited concentration-dependently the aggregation and ATP release of rabbit platelets caused by arachidonic acid (AA; 100 m...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(94)90142-2
更新日期:1994-07-01 00:00:00
abstract::The effect of the vasopressin analogue DDAVP on Factor VIII in plasma has been extensively studied in humans. To examine the effect of new and potentially better analogues a suitable animal model has to be established. In the present study trained Beagle dogs were injected with DDAVP and the time course of the Factor ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(87)90363-x
更新日期:1987-09-01 00:00:00
abstract::Urokinase-type plasminogen activator (uPA) plays a central role in many aspects of cellular regulation, such as fibrinolysis, cell migration, and metastasis. The uPA induction by inflammatory stimuli such as IL-1, TNFalpha, and lipopolysaccharide (LPS) has been reported in a number of human cells. We examined the effe...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(99)00174-7
更新日期:2000-03-01 00:00:00
abstract::The procoagulant cellular activity (PCA) of leukemic cells was evaluated, before and after endotoxin stimulation, in 38 patients with acute leukemia at presentation subdivided according to the FAB classification. In the M4 and M5 subgroups the stimulated leukemic cells showed a significant increase in the production o...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(87)90317-3
更新日期:1987-03-01 00:00:00
abstract::Evidence is presented for in vitro high affinity binding of serotonin (5-HT) by beta-thromboglobulin (beta TG) and platelet factor 4 (PF4) from human blood. Results include: 1) identification by radioimmunoassay of PF4 in specifically bound material obtained by 5-HT affinity chromatography of human platelet extracts; ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(83)90044-0
更新日期:1983-02-01 00:00:00
abstract:INTRODUCTION:Colorectal cancer (CRC) is often complicated by thromboembolic episodes. It has been recognized that blood coagulation proteins play a role in cancer progression. An important inhibitory mechanism is provided by the protein C (PC) system consisting of PC, protein S (PS) and thrombomodulin (TM). Recently, n...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2009.09.011
更新日期:2010-03-01 00:00:00
abstract::In the treatment of unstable coronary artery disease, direct thrombin inhibitors have shown no or only limited benefit as compared with heparin, despite theoretical advantages. One explanation may be that the direct thrombin inhibitors to a greater extent than heparin have an inhibiting effect on the generation and ac...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(99)00029-8
更新日期:1999-07-15 00:00:00
abstract::Aggregation responses of platelet-rich plasma (PRP) from monocrotaline pyrrole (MCTP)-treated rats were examined to help elucidate the role of platelets in MCTP-induced pulmonary hypertension. PRP from rats treated one or four days earlier with MCTP (5 mg/kg, i.v.) or vehicle exhibited the same slope and maximum aggre...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(83)90168-8
更新日期:1983-11-01 00:00:00
abstract::Previous studies indicated that human placental anticoagulant protein, a member of the lipocortin family, prolonged the clotting time of normal plasma when clotting was induced by brain thromboplastin or by kaolin in the presence of cephalin and calcium. Using a two-stage amidolytic assay to assess factor X activation...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(87)90402-6
更新日期:1987-11-15 00:00:00
abstract::The sequel of survivors after hospitalisation for an acute myocardial infarction was studied during the following 15 years in 48 patients treated with streptokinase and in 41 controls. No significant difference was seen in the morbidity or mortality during the first 6 months nor in the mortality thereafter. ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(84)90233-0
更新日期:1984-08-15 00:00:00
abstract::There is now compelling evidence that use of oral contraceptives and postmenopausal hormonal therapies containing various estrogens is associated with a weak, but clinically relevant risk of both arterial and venous thrombosis. The increased risk is related to type and dose of both estrogen and combined progestagen an...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/S0049-3848(13)70009-4
更新日期:2013-01-01 00:00:00