Abstract：
:Selective serotonin reuptake inhibitors (SSRIs) have been reported to reduce platelet aggregation induced by ADP. ADP induces platelet aggregation through two purinergic receptors P2Y1 and P2Y12. We characterized the inhibitory properties of SSRIs on ADP-induced platelet aggregation and investigated the effects of SSRIs on the signaling pathways downstream of P2Y1 and P2Y12 receptors. Specific antagonists were used to evaluate which purinergic receptor-mediated aggregation was influenced by SSRIs. The primary phase of ADP-induced aggregation was not inhibited by citalopram. Citalopram failed to influence ADP-induced platelet shape change, intracellular calcium mobilization and the early phosphorylation of PKCα. Differently, citalopram inhibited the secondary phase of ADP-induced platelet aggregation in a concentration-dependent manner. Other SSRIs, including fluoxetine and sertraline, exhibited the same anti-platelet effects. Under P2Y1 blockade, citalopram inhibited platelet aggregation and integrin αIIbβ3 activation in response to ADP, indicating that citalopram inhibited P2Y12-mediated aggregation. Citalopram concentration-dependently inhibited the phosphorylation of Akt, GSK3β, p38 MAPK and Syk induced by ADP, but showed no effect on the decrease of cAMP and VASP phosphorylation. With integrin αIIbβ3 blockade, however, the phosphorylation of Akt triggered by ADP was unaltered by the addition of citalopram. Taken together, under the stimulation of ADP, SSRIs inhibit the amplification of platelet aggregation secondary to the activation of P2Y12 receptor, and subsequently reduce the activation of the downstream molecules of the outside-in signaling pathways.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Tseng YL,Chiang ML,Lane HY,Su KP,Lai YCdoi
10.1016/j.thromres.2013.02.007subject
Has Abstractpub_date
2013-04-01 00:00:00pages
325-32issue
4eissn
0049-3848issn
1879-2472pii
S0049-3848(13)00050-9journal_volume
131pub_type
杂志文章abstract：:Several sex-related differences in the incidence rate, clinical presentation and outcomes of VTE were recently investigated. Gender-related risk factors, such as the use of oral contraceptives (OC) and pregnancy, in particular in women with a thrombophilic state, are associated with an increased risk of venous thrombo...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/S0049-3848(11)70023-8
更新日期：2011-02-01 00:00:00
abstract：INTRODUCTION:Conventional Vacutainer® Sodium Citrate blood collection tubes contain a relatively high concentration of contaminating magnesium ions, which may result in shortening of the prothrombin time (PT) and the International Normalized Ratio (INR). Recently the manufacturer of Vacutainer® Sodium Citrate tubes int...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2014.04.016
更新日期：2014-07-01 00:00:00
abstract：:The use of oral contraceptives (OCs) has been associated with vascular complications. The mechanism(s) by which OCs predispose to thrombotic events remains unclear. Recent studies have demonstrated that postmenopausal (PM) women who take estrogen replacement therapy (ERT) have a decreased incidence of myocardial infar...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(93)90043-n
更新日期：1993-01-15 00:00:00
abstract：:In a double-blind, placebo-controlled trial of 40 patients requiring aortocoronary vene transplant surgery, prostacyclin (PGI2) was infused in a dose of 8 ng/kg/min throughout cardiopulmonary bypass. When compared with the placebo-group, the patients treated with PGI2 were found to have significantly higher platelet c...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(83)90093-2
更新日期：1983-11-15 00:00:00
abstract：:Fibrinogen has been reported to interact with phospholipid; however, the properties of this binding interaction have not been characterized. Purified preparations of human fibrinogen bound to small unilamellar vesicles containing phosphatidylserine (PS) as measured by light scattering and radioisotope filtration. Bind...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(99)00058-4
更新日期：1999-09-15 00:00:00
abstract：:It has long been recognised that the function of platelets in health and disease span far beyond their roles in haemostasis and thrombosis. The observation that tumour cells induce platelet aggregation was followed by extensive experimental evidence linking platelets to cancer progression. Aggregated platelets coat tu...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/S0049-3848(12)70013-0
更新日期：2012-04-01 00:00:00
abstract：INTRODUCTION:Atypical hemolytic uremic syndrome (aHUS) is a rare thrombotic microangiopathy (TMA), characterized by micro-angiopathic hemolytic anemia, thrombocytopenia, and renal failure. In more than half of cases, genetic defects leading to overactivation of the alternative complement system have been identified. In...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2020.06.016
更新日期：2020-10-01 00:00:00
abstract：INTRODUCTION:High RDW values are associated with adverse prognosis in many clinical conditions including short and medium term outcome of patients with ST Elevation Myocardial Infarction (STEMI). The aim of the present study was to evaluate the association between RDW and long term mortality in STEMI patients undergoin...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2014.08.016
更新日期：2014-11-01 00:00:00
abstract：INTRODUCTION:Dermatan sulfate, a sulfated glycosaminoglycan, acts as an anticoagulant by accelerating the inhibition of thrombin by heparin cofactor II. MATERIALS AND METHODS:A low molecular mass dermatan sulfate was obtained by peroxy-radical depolymerization from a parent dermatan sulfate. Chemical characterization ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2007.09.001
更新日期：2008-01-01 00:00:00
abstract：:Arterial thrombosis and venous thromboembolism (VTE) are traditionally considered two different entities. However, patients with unprovoked VTE are at higher risk of developing subclinical and overt atherosclerosis compared to healthy controls challenging these distinctions. Obesity may explain the association between...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2020.04.035
更新日期：2020-07-01 00:00:00
abstract：:To estimate the effect of dilazep in preventing restenosis after a transluminal angioplasty, we have attempted an animal experiment in which the efficacy of drug was tested on an angioplastic injury superimposed on to atherosclerotic lesions. Using a modification of Block's method, atherosclerotic lesions first were m...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(89)90122-9
更新日期：1989-06-15 00:00:00
abstract：INTRODUCTION:In reconstructive head and neck cancer surgery, tissue flaps are transferred to the surgical defect and revascularized by anastomosis of small vessels. Cancer patients are in a hypercoagulable state with high risk of peri- and postoperative thrombotic events. Thrombosis to the tissue flap anastomoses or mi...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/S0049-3848(16)30195-5
更新日期：2016-04-01 00:00:00
abstract：:Heparin cofactor II (HCII) is a specific thrombin inhibitor; its inhibitory activity is stimulated by heparin (Hep) and dermatan sulfate (DS). Vitronectin (VN), a heparin binding adhesive glycoprotein present in plasma and extracellular matrix, has been shown to decrease the stimulatory effect of Hep but not of DS on ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(92)90310-7
更新日期：1992-06-01 00:00:00
abstract：:Platelet adherence and aggregation are important in the development of ischemic sequelae in atherosclerosis. To directly examine platelet interaction with plaque, everted, deendothelialized aortic fibrous plaques from Watanabe-heritable hyperlipidemic rabbits were exposed to flowing human blood in an annular perfusion...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(89)90040-6
更新日期：1989-04-15 00:00:00
abstract：:Venous thromboembolism (VTE) is a common complication in patients with malignant disease. In addition to well-established acquired risk factors for VTE, several genetic risk factors, mainly related to the haemostatic system, are known to influence thrombotic risk. However, the contribution of gene abnormalities to thr...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(03)00179-8
更新日期：2003-02-15 00:00:00
abstract：:Use of low molecular weight heparins (LMWHs) is now generally accepted as an ideal method of prophylaxis against postoperative venous thromboembolism. However, the long-term effects on the development of postthrombotic deep vein insufficiency are not known. Every patient developing such complications consumes a vast a...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/0049-3848(95)00232-4
更新日期：1996-01-01 00:00:00
abstract：:A series of 8 analogues of platelet-activating factor (PAF) in which both the acetyl group and the polar head are changed, were tested for biological activities on human and rabbit platelets in plasma. Two of these compounds, 1-O-hexadecyl-2-O-ethyl-rac-glycero-3-phosphoric acid-5'-trimethylammoniumpentyl ester and 1-...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(88)90244-7
更新日期：1988-02-15 00:00:00
abstract：OBJECTIVE:To investigate the risk of venous thromboembolism (VTE) among patients with autoimmune hemolytic anemia (AIHA). METHODS:We conducted a systematic review and meta-analysis of observational studies that reported odds ratio, relative risk, hazard ratio or standardized incidence ratio comparing the risk of VTE i...
journal_title：Thrombosis research
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.thromres.2015.09.004
更新日期：2015-11-01 00:00:00
abstract：INTRODUCTION:Unfractionated heparin (UFH) does not cross the placenta and has demonstrated utility in the prevention and treatment of thrombosis during pregnancy. Limited information is available to guide initiation and monitoring of therapeutic UFH targeting an anti-Xa concentration of 0.3-0.7 u/ml during pregnancy. T...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2009.07.014
更新日期：2010-05-01 00:00:00
abstract：INTRODUCTION:Low levels of plasminogen activator inhibitor type 1 (PAI-1) have been associated with increased risk for perioperative bleeding in some case reports. The aim of this study was to investigate prospectively whether low PAI-1 activity increases the risk for perioperative bleeding in patients undergoing trans...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2006.06.014
更新日期：2007-01-01 00:00:00
abstract：:Four novel polymorphisms were identified in the fibrinogen gene cluster. Three of them were localized in the promoter regions of the Aalpha-chain (alpha -128 C/G, alpha -58 G/A) or the gamma-chain (gamma -239 A/G) gene, while the remaining one was identified in intron 9 of the gamma-chain gene (gamma 7792 C/T). Genoty...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(01)00329-2
更新日期：2001-08-15 00:00:00
abstract：:A dimorphism in PROS1 gene (c.A2,001G, p.Pro667Pro) has been associated with significantly reduced levels of both free and total protein S in carriers of the GG genotype. It is not known how the GG genotype could influence PS levels in normals, whether it could influence the levels of protein S in carriers of mutation...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2006.10.014
更新日期：2007-01-01 00:00:00
abstract：:The action of AD6 as an anti-thrombotic agent was studied in a model of coronary artery thrombosis and on platelet aggregation in the dog. AD6 (10-100 microM) in vitro inhibited aggregation induced by ADP, epinephrine, collagen and PAF (platelet aggregating factor) used at their threshold concentration for maximal agg...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(85)90163-x
更新日期：1985-08-15 00:00:00
abstract：BACKGROUND:The diagnostic work-up for heparin induced thrombocytopenia (HIT) can take several days. Consequently patients may be speculatively switched onto replacement anticoagulant therapy before a diagnosis is confirmed. On-demand immunoassay diagnostic testing enables timely treatment decisions, based on test resul...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2016.01.025
更新日期：2016-04-01 00:00:00
abstract：:One of the primary and most serious treatment-related complications in haemophilia A is the formation of anti-factor VIM (FVIII) antibodies, which significantly impacts patient care. For these patients, immunomodulatory therapy becomes important to induce immunological tolerance to FVIII. Immune tolerance induction (I...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/S0049-3848(08)70003-3
更新日期：2008-01-01 00:00:00
abstract：:Two anti-cardiolipin antibody (ACA)-producing cell lines were established, using Epstein-Barr virus transformation followed by a repeated cluster-picking from the same individual with antiphospholipid syndrome who had a history of 8 consecutive fetal losses and deep venous thrombosis. Characterization of the two ACAs ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(94)90037-x
更新日期：1994-04-01 00:00:00
abstract：:Therapeutic developments have seen significant improvement in mortality over the past thirty years for breast, colorectal and prostate cancer. Lung cancer is the commonest cancer in the world, but has seen little improvement in survival figures. The role of low-molecular-weight heparin (LMWH) in improving cancer survi...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/S0049-3848(12)70029-4
更新日期：2012-04-01 00:00:00
abstract：:The extrinsic fibrinolytic system was assessed among 124 consecutive survivors of acute myocardial infarction below 70 years of age. In samples drawn 3 months after discharge from hospital, the PAI-1 levels were higher and the tPA activities were lower than among elderly healthy controls. In contrast, the AMI survivor...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(87)90428-2
更新日期：1987-12-15 00:00:00
abstract：OBJECTIVES:Screening of patients with sepsis for disseminated intravascular coagulation (DIC) has been recommended in several guidelines. However, DIC screening is still not widely accepted as an essential component of sepsis management, partly because of a lack of evidence that DIC screening has an effect on mortality...
journal_title：Thrombosis research
pub_type： 杂志文章,多中心研究
doi：10.1016/j.thromres.2017.11.023
更新日期：2018-01-01 00:00:00
abstract：INTRODUCTION:Antithrombin deficiency is associated with increased risk of venous thromboembolism (VTE). We aimed to identify variants causing antithrombin deficiency in a Danish population. MATERIALS AND METHODS:We performed Sanger sequencing and, in relevant cases, multiplex ligation-dependent probe amplification ana...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2019.01.022
更新日期：2019-03-01 00:00:00
