当前位置： SCI文献检索 > THROMBOSIS RESEARCH期刊下所有文献 > A comparative study of conventional versus new, magnesium-poor Vacutainer® Sodium Citrate blood collection tubes for determination of prothrombin time and INR.

A comparative study of conventional versus new, magnesium-poor Vacutainer® Sodium Citrate blood collection tubes for determination of prothrombin time and INR.

Abstract：

INTRODUCTION:Conventional Vacutainer® Sodium Citrate blood collection tubes contain a relatively high concentration of contaminating magnesium ions, which may result in shortening of the prothrombin time (PT) and the International Normalized Ratio (INR). Recently the manufacturer of Vacutainer® Sodium Citrate tubes introduced new tubes with a magnesium-poor stopper. The magnesium concentration in the new low-Mg tubes is significantly lower than that in the conventional plastic tubes. The purpose of the present study was to compare PT and INR determined in specimens drawn with the new tubes to those drawn with the conventional tubes. MATERIALS AND METHODS:Venous blood specimens were collected from 22 healthy persons and 65 patients treated with vitamin K-antagonists using conventional Vacutainer® Sodium Citrate tubes and new, low-Mg Vacutainer® tubes. PT and INR were determined with four thromboplastin reagents, i.e., three brands of recombinant human tissue factor and one brand of combined rabbit brain reagent. Magnesium concentrations were determined in the citrate plasmas with a colorimetric method. RESULTS:The differences in PT, INR and International Sensitivity Index (ISI) between the two tubes were significant when using three recombinant human thromboplastin reagents, but were not significant when using the rabbit thromboplastin. The PT and INR differences between the tubes correlated with the magnesium concentration differences (P<0.001). The INR bias between the four reagents was greater for specimens drawn with conventional tubes than the INR bias for specimens drawn with the new tubes. CONCLUSION:Agreement of INR between reagents is improved by using magnesium-poor tubes.

journal_name

Thromb Res

journal_title

Thrombosis research

authors

van den Besselaar AM,van Vlodrop IJ,Berendes PB,Cobbaert CM

doi

10.1016/j.thromres.2014.04.016

subject

Has Abstract

pub_date

2014-07-01 00:00:00

pages

187-91

issue

1

eissn

0049-3848

issn

1879-2472

pii

S0049-3848(14)00230-8

journal_volume

134

pub_type

杂志文章

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