Abstract：
:Two monoclonal antibodies against plasminogen and two against alpha-2-antiplasmin were characterized regarding their binding properties towards their respective native and modified antigens and towards the plasmin-alpha-2-antiplasmin complex. Using an ELISA system, the two monoclonal antibodies against plasminogen (MPW1PG, MPW2PG), reacted equally well with purified Glu-plasminogen; binding of one (MPW1PG) to the purified plasmin-alpha-2-antiplasmin complex was, however, more than three orders of magnitude weaker than that of the other. Similarly, both antibodies against alpha-2-antiplasmin (MPW2AP, MPW3AP) reacted equally well with native alpha-2-antiplasmin but binding of MPW3AP to the plasmin-alpha-2-antiplasmin complexes was more than one order of magnitude weaker than that of the other. These results were confirmed by immunoblotting by which it was made evident that the antiplasminogen antibody MPW1PG reacted only with the free or complexed Glu-form of plasminogen or plasmin, while MPW2PG reacted equally well with the Glu- and Lys-form. The alpha-2-antiplasmin antibody MPW2AP reacted with native and complexed alpha-2-antiplasmin while MPW3AP reacted only with the native antigen and with a 8,000 dalton cleavage product of the complex.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Hattey E,Wojta J,Binder BRdoi
10.1016/0049-3848(87)90311-2subject
Has Abstractpub_date
1987-03-01 00:00:00pages
485-95issue
5eissn
0049-3848issn
1879-2472pii
0049-3848(87)90311-2journal_volume
45pub_type
杂志文章abstract：:Clot lysis induced by streptokinase has been studied and the influences of pH and the concentrations of plasminogen, fibrinogen and buffer composition investigated. Variations in the concentrations of plasminogen strongly effect the SK apparent activity, maxima being obtained at different plasminogen concentrations li...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(91)90071-4
更新日期：1991-12-15 00:00:00
abstract：:Three commercial thrombin preparations used clinically to stop topical bleeding were studied. The specific activities of these preparations were 1,064 U/mg (human); 59 U/mg (bovine) and 147 U/mg (bovine). SDS-PAGE analysis of the human product produced one major band corresponding to a molecular weight of alpha-thromb...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(89)90102-3
更新日期：1989-02-01 00:00:00
abstract：INTRODUCTION:An individualised thromboprophylaxis was implemented in critically ill patients suffering from coronavirus disease 2019 (COVID-19) pneumonia to reduce mortality and improve clinical outcome. The aim of this study was to evaluate the effect of this intervention on clinical outcome. METHODS:In this mono-cen...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2020.07.038
更新日期：2020-10-01 00:00:00
abstract：:Von Willebrand disease (VWD) is classified into three primary subtypes; type 1 includes partial quantitative deficiency, type 2 (A, B, M and N) includes qualitative defects and type 3 includes virtually complete deficiency of von Willebrand factor (VWF). Type 2A includes variants with decreased platelet adhesion cause...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2007.03.014
更新日期：2007-01-01 00:00:00
abstract：:von Willebrand Factor (vWF) is released from endothelial cells. Increased vWF in the coronary circulation during cardiac surgery could be a potential indicator of coronary endothelial injury or stimulation, and thus a possible tool to evaluate regimens of myocardial protection. Release of vWF was investigated in 12 pa...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(94)90050-7
更新日期：1994-01-01 00:00:00
abstract：:The coumarin derivative AD6 is known to inhibit platelet aggregation and release and it possesses vasodilatory properties on coronary arteries of laboratory animals. Furthermore, the inhibition of the production of TxB2 from endogenous substrates after stimulation of human platelets with collagen has been demonstrated...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(87)90236-2
更新日期：1987-07-01 00:00:00
abstract：:The activity of tissue plasminogen activator-plasminogen activator inhibitor-1 (t-PA-PAI-1) complex to convert plasminogen to plasmin was examined by using fibrin autography and casein zymography where plasminogen was added or removed for casein layer. Fibrin autography was more sensitive to the activity of t-PA even ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(91)90279-6
更新日期：1991-07-01 00:00:00
abstract：:The complex relation between thrombotic thrombocytopenic purpura (TTP) and pregnancy is concisely reviewed. Pregnancy is a very strong trigger for acute disease manifestation in patients with hereditary TTP caused by double heterozygous or homozygous mutations of ADAMTS13 (ADisintegrin And Metalloprotease with Thrombo...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/S0049-3848(15)50437-4
更新日期：2015-02-01 00:00:00
abstract：INTRODUCTION:Patients with nonvalvular atrial fibrillation are at increased risk for systemic embolism, predominantly disabling stroke. To study how stroke and mortality rates vary with different degrees of anticoagulation reflected by the international normalised ratio (INR) we critically assess information from diffe...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2004.11.025
更新日期：2006-01-01 00:00:00
abstract：:The aim of this study was to compare the efficacy and safety of prolonged (35 days) thromboprophylaxis with a standard length (7 days) regimen of a low molecular weight heparin in patients undergoing total hip arthroplasty. The study was multicentre, randomised, double-blind, and prospective with two groups. Following...
journal_title：Thrombosis research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0049-3848(98)00018-8
更新日期：1998-03-15 00:00:00
abstract：:The candidate-gene approach can be used to locate and identify genetic variations that are associated with a particular phenotype. This gene-centric approach assumes that there exists important genetic variation within genes that can influence health. Identifying known genes which are candidates for the phenotype of i...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/S0049-3848(09)70139-2
更新日期：2009-01-01 00:00:00
abstract：:Upon incubation for 24 hours with [3H]arachidonic acid (AA, 1 mu Ci/ml), cultured endothelial cells from human umbilical vein incorporated one half of the added radioactivity, mostly into phospholipids (83% of the total cell radioactivity). Distribution of the label between the various phospholipid classes was found t...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(84)90068-9
更新日期：1984-04-15 00:00:00
abstract：:Experimental disseminated intravascular coagulation (DIC) can be induced by 4-h sustained infusion of endotoxin in a dose of 100 mg/kg in rats. The experimental model of DIC in rats was used to study the preventive effect of dipyridamole against DIC. Before the infusion of endotoxin, 0.5, 5.0 or 50.0 mg/kg of dipyrida...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(83)90216-5
更新日期：1983-03-15 00:00:00
abstract：INTRODUCTION:Extracellular histones are reported to increase thrombin generation in the plasma and induce endothelial cell death in vitro. These effects of histones were suggested to involve histone-induced inhibition of TM-dependent activated protein C (APC) generation. Therefore, we hypothesized that TM alfa, a recom...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2017.10.019
更新日期：2017-12-01 00:00:00
abstract：:Diagnosis and risk stratification of patients with acute coronary syndromes remain extremely important in order to avoid unnecessary hospitalizations on the one hand, and to improve prognosis of these patients on the other. For diagnosis of acute coronary syndromes, an ECG should be obtained at rest, troponin T and I ...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/s0049-3848(01)00298-5
更新日期：2001-09-30 00:00:00
abstract：:Blackfoot disease is a unique endemic and chronic progressive arteriosclerotic vascular disease in southwest area of Taiwan. In this study, we determined the plasma levels of tissue plasminogen activator (tPA), plasminogen activator inhibitor (PAI), urokinase plasminogen activator (uPA), and von Willebrand factor (vWF...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(93)90188-t
更新日期：1993-11-01 00:00:00
abstract：BACKGROUND:Cardiovascular disease remains the leading cause of morbidity and premature mortality in most industrialized countries as well as in developing nations. A pro-oxidative state appears to promote and/or exacerbate vascular disease complications. Furthermore, a state of low-grade chronic inflammation can promot...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2010.07.003
更新日期：2010-10-01 00:00:00
abstract：INTRODUCTION:Electronic consultations (eConsults) are asynchronous, consultative electronic-based communications that are aimed to improve efficient and timely access to specialist advice. Our study aim was to evaluate the use and impact of the Canadian Champlain BASE™ (Building Access to Specialists through eConsultat...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2017.11.002
更新日期：2017-12-01 00:00:00
abstract：:Cancer-associated arterial thrombotic events (ATEs) are increasingly recognized in specific malignancies and in association with the expanding armamentarium of novel chemotherapeutic agents. The improved cancer survival led to cardiovascular complications becoming clinically relevant many years after cancer diagnosis....
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2018.01.003
更新日期：2018-04-01 00:00:00
abstract：:Beta-thromboglobulin (beta-TG) is a platelet-specific protein present in the alpha-granules and secreted into the surrounding medium on cell activation. The sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of platelet releasate after inhibition of metalloproteinases with ethyleneglycol-bis-(beta-a...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(02)00135-4
更新日期：2002-05-15 00:00:00
abstract：INTRODUCTION:To validate the diagnostic criteria for disseminated intravascular coagulation (DIC) established by the Japanese Association for Acute Medicine (JAAM) at an early stage of trauma and to evaluate the hypothesis that the JAAM criteria can diagnose DIC with a higher sensitivity than the International Society ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2009.06.036
更新日期：2009-12-01 00:00:00
abstract：BACKGROUND:Sampling small volumes of blood may be necessary, particularly in pediatric patients, or in case of difficult or recurrent venipunctures. METHODS:Routine hemostasis test results evaluated in partial- and full-draw evacuated polymer tubes obtained in 4 centers were compared. RESULTS:No relevant discrepancy ...
journal_title：Thrombosis research
pub_type： 杂志文章,多中心研究
doi：10.1016/j.thromres.2010.08.029
更新日期：2010-12-01 00:00:00
abstract：INTRODUCTION:Sepsis is frequently complicated by disseminated intravascular coagulation (DIC), which promotes multiple organ dysfunctions and significantly increase the mortality of patients with sepsis. How bacteria cause DIC is not fully understood. Outer membrane vesicles (OMVs) are membrane-enclosed microvesicles r...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2019.03.019
更新日期：2019-06-01 00:00:00
abstract：INTRODUCTION:It is currently unclear whether chronic kidney disease (CKD) and the decrease in renal function can influence the risk of venous thromboembolism (VTE) recurrence. MATERIALS AND METHODS:We performed an ambispective observational study on 409 patients with a previous episode of VTE. All the patients were in...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2017.10.011
更新日期：2017-12-01 00:00:00
abstract：:Forty women undergoing surgery under general anaesthesia for hyperplasia mammae were randomized to treatment with low dose heparin (5000 IU twice daily) or not. Preoperatively, and repeated on the 3. postoperative day, assays of euglobulin clot lysis time (ELT) after venous stasis, tissue plasminogen activator (t-PA) ...
journal_title：Thrombosis research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/0049-3848(87)90318-5
更新日期：1987-03-01 00:00:00
abstract：:This work was designed to investigate the influence of rat platelet adrenoceptors on the early thrombin-induced serotonin release. In washed platelets prelabeled with [3H]-serotonin, adrenaline and isoproterenol both inhibited, in a dose-dependent manner, the early thrombin-induced secretion of serotonin. Inhibitory r...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(85)90325-1
更新日期：1985-10-15 00:00:00
abstract：:Indirect genetic diagnosis using polymorphic DNA markers can be useful in large-scale screening programs, which is technically simpler, more rapid and amenable. The main objective of this study was to test the informativeness of two common intragenic markers (TaqI and XmnI) in Iranian haemophilia B families to detect ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2004.03.009
更新日期：2004-01-01 00:00:00
abstract：:The need for anticoagulation in dialysis patients is common and the incidence of venous thromboembolism (VTE) and atrial fibrillation in this population is high. While direct data are lacking on the management of anticoagulation in dialysis patients, careful weighing of risks and benefits on the basis of evidence from...
journal_title：Thrombosis research
pub_type： 杂志文章,实务指引
doi：10.1016/j.thromres.2005.03.031
更新日期：2006-01-01 00:00:00
abstract：:We investigated the alteration of heparan sulfate proteoglycans induced by thrombin in cultured vascular endothelial cells. Heparan sulfate proteoglycans, which were metabolically labeled with [3H] glucosamine and [35S] sulfate, were isolated by DEAE-Sephacel ion-exchange chromatography and characterized by molecular ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(97)00257-0
更新日期：1997-11-01 00:00:00
abstract：:Megakaryocyte colony formation was increased in 13 patients with ITP. Out of splenectomized ITP patients Colony Forming Unit-Megakaryocyte (CFU-Mk) number normalized in four but was lower than normal in the remaining two. The proportion of immature megakaryocytes was higher in ITP than in normals or in ITP patients af...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(91)90672-j
更新日期：1991-04-01 00:00:00
