Abstract:
:The activity of tissue plasminogen activator-plasminogen activator inhibitor-1 (t-PA-PAI-1) complex to convert plasminogen to plasmin was examined by using fibrin autography and casein zymography where plasminogen was added or removed for casein layer. Fibrin autography was more sensitive to the activity of t-PA even in the complex with PAI-1, but casein zymography was more sensitive to plasmin. The inhibitory activity of PAI-1 toward t-PA diminished when plasma was clotted, thus in the presence of fibrin. These results indicate that t-PA-PAI-1 complex has a catalytic activity even in the absence of fibrin, but the activity is enhanced in its presence.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Takada Y,Takada Adoi
10.1016/0049-3848(91)90279-6subject
Has Abstractpub_date
1991-07-01 00:00:00pages
169-77issue
1eissn
0049-3848issn
1879-2472journal_volume
63pub_type
杂志文章abstract::Activation of washed platelets in the presence of EDTA with either 1 U/ml of alpha-thrombin or 2 microM calcium ionophore (A23187) caused the release of one-third to one-half of the platelet factor VIII/von Willebrand factor (FVIII/vWF) into the supernatant. When calcium was present in excess, only 10% of the platelet...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(84)90282-2
更新日期:1984-09-01 00:00:00
abstract:INTRODUCTION:Venous thromboembolism (VTE) prophylaxis guidelines for non-surgical patients recommend VTE- and bleeding risk assessment to guide prophylactic strategies. These recommendations differ between guidelines and implementation is suboptimal. Assessing a guideline's implementability characteristics helps predic...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2018.05.028
更新日期:2018-08-01 00:00:00
abstract::The variant von Willebrand disease (vWd) variant type 2M (Vicenza) was identified in 13 patients of 7 unrelated families. 11 patients were from different parts of germany and 2 patients from Turkey. Hitherto this variant of vWd has been described only in two families originating from the province of Vicenza in Norther...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(97)00104-7
更新日期:1997-07-01 00:00:00
abstract::The procoagulant cellular activity (PCA) of leukemic cells was evaluated, before and after endotoxin stimulation, in 38 patients with acute leukemia at presentation subdivided according to the FAB classification. In the M4 and M5 subgroups the stimulated leukemic cells showed a significant increase in the production o...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(87)90317-3
更新日期:1987-03-01 00:00:00
abstract::Previous studies have indicated that the skin bleeding time test does not accurately reflect visceral bleeding time (BT). The present study examines the predictive value of the skin bleeding time for brain bleeding tendency. Sixteen Sprague-Dawley rats were divided into equal groups. The first group (controls) underwe...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(94)90282-8
更新日期:1994-12-15 00:00:00
abstract::To compare Canola a terrestrial (n-3) oil with fish oil (n-3) and common vegetable oil (n-6) in decreasing serum lipids and platelet aggregation, various oils were included in Chow-based diets and fed to rats in an eight-weeks feeding trial. The Chow diet contained 2% cholesterol along with either Canola, menhaden, sa...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(90)90420-h
更新日期:1990-08-01 00:00:00
abstract:OBJECTIVE:To characterize antithrombin (AT) levels in normal pregnancy. METHODS:We performed secondary analyses with data from 3 studies. Using a single measurement from each subject in the first analysis (cross-sectional), we correlated AT levels with gestational age from the middle of the second trimester throughout...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2014.07.025
更新日期:2014-09-01 00:00:00
abstract::An in vitro model of platelet adhesion, the annular perfusion chamber, was utilized to test the effects of Interleukin 1 on vascular thrombogenicity for platelets. De-endothelialized, everted human umbilical arteries were placed in cell culture media with or without Interleukin 1. The arteries were later perfused with...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(89)90235-1
更新日期:1989-11-15 00:00:00
abstract:BACKGROUND:The plasminogen activator inhibitor type 1 (PAI-1) 4G/5G polymorphism may have significance for PAI-1 expression. High levels of PAI-1 in breast cancer patients are associated with a poor prognosis. In this study, we analyzed the influence of the PAI-1 4G/5G polymorphism on tissue PAI-1 levels and its associ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2005.03.025
更新日期:2006-01-01 00:00:00
abstract::Platelet activation plays an important role in the development of sepsis. During sepsis, platelet activation leads to endothelial cell injury and promotes neutrophil extracellular trap and microthrombus formation, exacerbating septic coagulation and inflammatory reactions. The resultant induction or aggravation of dis...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/j.thromres.2018.04.007
更新日期:2018-06-01 00:00:00
abstract::Thrombocytopenia is a common finding in cancer patients and can have different and/or multifactorial pathogenesis. While in solid tumors it occurs often as a consequence of chemotherapy treatment, it is frequently observed at diagnosis in patients with hematological malignancies being aggravated to a potentially life-...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/j.thromres.2018.02.001
更新日期:2018-04-01 00:00:00
abstract:INTRODUCTION:Pathogenesis of idiopathic pulmonary arterial hypertension (iPAH) includes endothelial dysfunction and in situ thrombosis. A hypercoagulable state has also been postulated but never demonstrated. Our objective was to determine whether patients with iPAH had a hypercoagulable state using calibrated automate...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2010.08.020
更新日期:2010-12-01 00:00:00
abstract::Antithrombin (AT) is a serpin capable of trapping thrombin (IIa) in a stable and covalent complex. Complex formation is prevented by leukocyte elastase (LE) cleavage near the AT reactive centre. We mutated the known LE cleavage sites of AT to explore the possibility of producing an LE-resistant AT molecule. Initially,...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(97)00228-4
更新日期:1997-10-15 00:00:00
abstract::Patients with essential hypertension (EH) and hyperhomocysteinemia (HHCY) suffer from more increased thrombotic events than those in EH alone. However, the underlying mechanisms for this effect are not well understood. This study hypothesized that neutrophil extracellular trap (NET) releasing may be triggered by HHCY ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2020.10.028
更新日期:2021-01-01 00:00:00
abstract::Pulmonary embolism (PE) is a common, lethal yet treatable disease. The clinical diagnosis of PE remains to be a problem due to the nonspecific presenting signs, symptoms, electrocardiographic findings, arterial blood gas abnormalities and chest X-ray changes. Despite these nonspecific clinical findings, clinicians are...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/s0049-3848(01)00326-7
更新日期:2001-09-15 00:00:00
abstract::Experimental disseminated intravascular coagulation (DIC) can be induced by 4-h sustained infusion of endotoxin in a dose of 100 mg/kg in rats. The experimental model of DIC in rats was used to study the preventive effect of dipyridamole against DIC. Before the infusion of endotoxin, 0.5, 5.0 or 50.0 mg/kg of dipyrida...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(83)90216-5
更新日期:1983-03-15 00:00:00
abstract::The effects and mechanism of action of a phospholipase C (PLC) from Pseudomonas aeruginosa on human platelet rich plasma were examined to better understand the interaction of PLC with human platelets. PLC caused platelet aggregation in a concentration-dependent manner. Enzymatic activity of PLC was necessary for aggre...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(88)90115-6
更新日期:1988-09-01 00:00:00
abstract::In this paper, we demonstrated that the procoagulant action of Lonomia achelous (Cramer) is due in part to a component that activates prothrombin. The activation by crude venom and Fractions obtained by gel filtration on Sephadex G-75 is not dependent of phospholipid, Ca++ or Factor V. The activation of prothrombin by...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(92)90187-f
更新日期:1992-05-01 00:00:00
abstract::The inability of the heparin-antithrombin complex to inhibit fibrin-bound thrombin limits the utility of heparin for treatment of arterial thrombosis. In contrast with heparin, melagatran, a direct thrombin inhibitor (DTI), is equally effective at inhibiting fluid-phase thrombin and thrombin bound to fibrin. This refl...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/s0049-3848(03)00251-2
更新日期:2003-07-15 00:00:00
abstract:OBJECTIVES:In this randomized management study, we examined the safety of withholding anticoagulation on the basis of negative impedance plethysmography (IPG) compared to negative contrast venography (CV) in symptomatic patients with a first episode of clinically suspected deep vein thrombosis (DVT), and we determined ...
journal_title:Thrombosis research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0049-3848(01)00222-5
更新日期:2001-04-01 00:00:00
abstract:BACKGROUND:Atherosclerosis occurs as a result of a chronic inflammatory response in the arterial wall associated with an increased uptake of low-density lipoprotein by macrophages and the subsequent transformation of this lipoprotein into foam cells. It has been found that miR-188-3p can suppress autophagy and myocardi...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2018.09.043
更新日期:2018-11-01 00:00:00
abstract::The antithrombotic actions of selective factor Xa (FXa) inhibitors, recombinant tick anticoagulant peptide (rTAP) and DX-9065a, were evaluated in experimental thrombosis models in anesthetized rats. In the first model, thrombosis was induced by exposing flowing blood to a silk thread anchored in an arteriovenous (AV) ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(96)00112-0
更新日期:1996-07-15 00:00:00
abstract::The effect of the combined oral administration of pentoxifylline (pof) and low dose acetylsalicylic acid (ASA) was evaluated with the help of the laser-induced thrombosis in rat mesenteric arterioles. Laser-induced thrombosis is inhibited in a dose-dependent way by both drugs. The administration of ASA, either simulta...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(87)90299-4
更新日期:1987-04-15 00:00:00
abstract::Rabbit platelets pretreated with CV-3988 had reduced responsiveness to platelet activating factor(PAF) even after the platelets had been washed with a buffer. This was due to its tight binding to receptor sites for PAF, but not conformational changes of the receptor, because the platelets that were pretreated with CV-...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(87)90215-5
更新日期:1987-04-01 00:00:00
abstract:INTRODUCTION:Apoptotic cell death is a highly regulated genetic program, which has been observed in mature megakaryocytes fragmenting into platelets. The clock gene Per2, a key component of core clock oscillator, was involved in affecting both cell cycle control and apoptosis. Thus, loss of Per2 function may be conside...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2010.11.025
更新日期:2011-02-01 00:00:00
abstract::Ethamsylate possesses antihemorrhagic properties, but whether or not it directly activates blood platelets is unclear. Here we investigated the platelet activation potential of ethamsylate, by measuring membrane P-selectin expression with flow cytometry in human whole blood and also by immunofluorescence imaging of is...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(02)00353-5
更新日期:2002-09-15 00:00:00
abstract::Human platelets synthesize nitric oxide (NO) through an endothelial-type NO synthase (ecNOS) activated also by substances enhancing 3',5'-cyclic adenosine monophosphate (cAMP) concentrations, such as catecholamines, beta-adrenoceptor agonists and adenosine. To verify whether cAMP directly activates ecNOS through the c...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2004.06.036
更新日期:2004-01-01 00:00:00
abstract::Bovine myelin basic protein (BP) induced a shape change and endogenous phosphorylation of a 45,000 (45K) molecular weight protein of intact human platelets. This effect occurred rapidly over an effective concentration range of 5-100 microM BP. BP peptides encompassing residues 1-42, 43-88, and 89-169 from the BP molec...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(82)90090-1
更新日期:1982-03-15 00:00:00
abstract:CONTEXT:Recently, there has been an increasing number of reports of incidental pulmonary embolism (PE) in patients undergoing chest computer tomography (CT) for reasons other than the research of suspected PE. Natural history of incidental PE remains unclear. OBJECTIVES:To estimate the prevalence of incidental PE, to ...
journal_title:Thrombosis research
pub_type: 杂志文章,meta分析
doi:10.1016/j.thromres.2010.03.016
更新日期:2010-06-01 00:00:00
abstract::The influence of red blood cells on platelet aggregation has recently been a subject of considerable interest. We have studied the effect of red cells on spontaneously formed platelet aggregates in rotating vials at 37 degrees C. Platelet aggregation was quantified by measuring the fall in number of single platelets w...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(85)90150-1
更新日期:1985-05-01 00:00:00