Abstract：
:Heparanase is an endo-beta-D-glucuronidase capable of cleaving heparan sulfate (HS) side chains of heparan sulfate proteoglycans (HSPG) on cell surfaces and the extracellular matrix, activity that is strongly implicated in tumor metastasis and angiogenesis. Evidence was provided that heparanase over-expression in cancer cells results in a marked increase in tissue factor (TF) levels. Likewise, TF was induced by exogenous addition of recombinant heparanase to tumor cells and primary endothelial cells, induction that was mediated by p38 phosphorylation and correlated with enhanced procoagulant activity. TF induction was further confirmed in heparanase over-expressing transgenic mice and correlated with heparanase expression levels in leukemia patients. Heparanase was also found to be involved in the regulation of tissue factor pathway inhibitor (TFPI). A physical interaction between heparanase and TFPI was demonstrated, suggesting a mechanism by which secreted heparanase interacts with TFPI on the cell surface, leading to dissociation of TFPI from the cell membrane and increased coagulation activity, thus further supporting the local pro-thrombotic function of heparanase. Data indicate a possible involvement of heparanase in early miscarriages and point to a regulatory effect on TFPI and TFPI-2 in trophoblasts. As heparins are strong inhibitor of heparanase, in view of the effect of heparanase on TF, the role of heparins anticoagulant-activity may potentially be expanded. Taking into account the pro-metastatic and pro-angiogenic functions of heparanase, its over-expression in human malignancies and abundance in platelets, its involvement in the coagulation machinery is an intriguing novel arena for further research.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Nadir Y,Brenner Bdoi
10.1016/S0049-3848(10)70018-9subject
Has Abstractpub_date
2010-04-01 00:00:00pages
S72-6eissn
0049-3848issn
1879-2472pii
S0049-3848(10)70018-9journal_volume
125 Suppl 2pub_type
杂志文章abstract：:Seven males with atherosclerotic disease received daily 1.6 g of the thromboxane antagonist BM 13.177 in two separate oral dosages over a period of four days. The drug significantly reduced elevated plasma levels of thromboxane B2, beta-thromboglobulin and platelet factor 4, whereas thromboxane B2 generation was only ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(84)90229-9
更新日期：1984-08-15 00:00:00
abstract：:In spite of numerous studies on the involvement of fibrinogen in transendothelial migration of leukocytes and thereby inflammation, there is still no clear understanding of which fibrin(ogen) species can stimulate leukocyte transmigration. Although we have previously proposed that interaction of fibrin with the VLDL r...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2017.11.007
更新日期：2018-02-01 00:00:00
abstract：:Experience with tranexamic acid, an indirect fibrinolytic inhibitor, started as soon as it was released from Shosuke Okamoto's lab in the early 1960s. It was first prescribed to females with heavy menstrual blood loss and to patients with hereditary bleeding disorders. Soon the indications were widened to elective sur...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2014.11.012
更新日期：2015-02-01 00:00:00
abstract：:Sandwich ELISA systems were developed to measure human von Willebrand factor (vWF). In one system the microtitre plates were coated with goat F(ab')2 to vWF. The F(ab')2 different molecular forms in the soluble phase. VWF antigen bound by the F(ab')2 antibody was subsequently determined by using horse-radish peroxidas...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(86)90047-2
更新日期：1986-07-01 00:00:00
abstract：:The coumarin derivative AD6 is known to inhibit platelet aggregation and release and it possesses vasodilatory properties on coronary arteries of laboratory animals. Furthermore, the inhibition of the production of TxB2 from endogenous substrates after stimulation of human platelets with collagen has been demonstrated...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(87)90236-2
更新日期：1987-07-01 00:00:00
abstract：INTRODUCTION:Although investigation for JAK2 V617F mutation is recommended in patients presenting with splanchnic venous thrombosis (SVT), no specific clinical advice is given to SVT patients presenting without myeloproliferative neoplasms (MPN) and JAK2 V617F mutation. In MPN-free SVT patients, to investigate the clin...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2013.07.014
更新日期：2013-08-01 00:00:00
abstract：:The immunogenic potential of natural and recombinant hirudin was investigated using baboons. Animals received 4 intravenous hirudin injections of 1,000 antithrombin units/kg at certain times (day 1, 3, 8 and 42) and were checked for an immune response. None of the tests performed (in vitro histamine-release, ELISA for...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(91)90167-u
更新日期：1991-01-01 00:00:00
abstract：:To determine whether homocysteine, a risk factor for possible endothelial cell dysfunction, procoagulant effects and premature vascular disease but with a potential nutritional role for fetal and neonatal metabolism and development, is elevated in women at time of elective caesarean section with nitrous oxide general ...
journal_title：Thrombosis research
pub_type： 临床试验,杂志文章
doi：10.1016/j.thromres.2003.11.004
更新日期：2003-01-01 00:00:00
abstract：INTRODUCTION:Guidelines recommend the assessment of stroke and bleeding risk before initiating warfarin anticoagulation in patients with atrial fibrillation. Many of the elements used to predict stroke also overlap with bleeding risk in atrial fibrillation patients and it is tempting to use stroke risk scores to effici...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2014.05.034
更新日期：2014-08-01 00:00:00
abstract：:The immunoglobulin superfamily member EMMPRIN (CD147) plays an important role in a number of organ systems, including the cardiovascular system. Here we review the contemporary understanding of EMMPRIN and EMMPRIN-associated sequelae in the course of atherosclerosis. A significant body of data documents the pivotal ro...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2013.04.017
更新日期：2013-06-01 00:00:00
abstract：:An experimental in vitro model of the hemodynamics that occur in atrial fibrillation (AFib) in the left atrial appendage (LAA) was developed to study changes in human endothelial cell thrombotic potential. We applied human-derived sinus rhythm and AFib hemodynamic shear stress patterns to primary human endothelial cel...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2016.04.022
更新日期：2016-07-01 00:00:00
abstract：:Human apolipoprotein B was purified by barium sulphate adsorption, subsequent delipidation and gel filtration. The protein was then reconstituted in soya bean lecithin and its inhibitory effect towards thromboplastin was assayed by incubation with rabbit brain thromboplastin. The use of an antibody against human apoli...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(92)90033-7
更新日期：1992-10-15 00:00:00
abstract：:The two objectives of the study were to determine whether a procedure could be developed for measuring cancer procoagulant (CP) activity in human serum and if this procedure provided a method for distinguishing people with cancer from those without cancer. A procedure was developed for processing human serum such that...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(89)90256-9
更新日期：1989-11-01 00:00:00
abstract：INTRODUCTION:The epidemiology of tamoxifen and venous thromboembolism (VTE) is not well understood, and most data on tamoxifen toxicity are from adjuvant clinical trials. This study examined the relationship between the duration of tamoxifen use in female patients with breast cancer and the risk of VTE in a large popul...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2011.11.025
更新日期：2012-07-01 00:00:00
abstract：:Tissue factor (TF) is an integral membrane glycoprotein that serves as a cofactor for blood coagulation factor VIIa. The induction of TF on the surface of endothelial cells is initiated by various kinds of stimuli including lipopolysaccharide (LPS), interleukin-1 beta (IL-1 beta), and tumor necrosis factor alpha (TNF ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(94)90002-7
更新日期：1994-04-15 00:00:00
abstract：:There is a close interrelation between cancer and haemostasis, which is characterized by changes in the haemostatic system, mostly an activation of coagulation in cancer patients. Venous thromboembolism (VTE) is a frequent complication of cancer and in particular of anticancer therapy and is associated with significan...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/S0049-3848(10)70005-0
更新日期：2010-04-01 00:00:00
abstract：BACKGROUND:Cerebral vein thrombosis (CVT) is a rare, life-threatening disease affecting one adult per 100,000 per year. Genetic risk factors are deficiencies of the natural anticoagulant proteins antithrombin, protein C, protein S or single nucleotide polymorphisms such as factor V Leiden and prothrombin 20210A. In 20%...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2018.06.011
更新日期：2018-09-01 00:00:00
abstract：:Lipopolysaccharide-containing outer membrane vesicles (OMV-LPS) which are spontaneously released from Neisseria meningitidis during logarithmic growth were studied for their ability to induce procoagulant (tissue factor), profibrinolytic (urokinase-type plasminogen activator) and antifibrinolytic (plasminogen activato...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(01)00256-0
更新日期：2001-05-15 00:00:00
abstract：INTRODUCTION:Unicompartmental knee arthroplasty (UKA) has increased due to potential favourable complication rates when compared to total knee arthroplasty (TKA). Although venous thromboembolism (VTE) is a well-documented complication after TKA limited data is available after UKA and mostly presented as secondary findi...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2020.07.002
更新日期：2020-11-01 00:00:00
abstract：:Von Willebrand Factor is a multimer produced by endothelial cells and megakaryocytes, being stored in intracellular organelles, such as the Weibel-Palade bodies and alpha-granules in endothelial cells and platelets, respectively. This molecule acts as a carrier protein for factor VIIIc, involved in the intrinsic pathw...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(99)00122-x
更新日期：2000-01-15 00:00:00
abstract：:Algal fucoidans possess a wide variety of biological activities, including anticoagulation and antithrombosis, making them potential candidates for clinical use. We assessed the antiaggregant, anticoagulant and antithrombotic activities and the underlying mechanism of the low- and high-molecular weight fucoidans from ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2010.02.011
更新日期：2010-05-01 00:00:00
abstract：:Homocystinuria due to cystathionine-beta-synthase deficiency (CBS-def-HOCY) initially often presents with vascular disorders, e.g. thromboembolic events. The measurement of vascular endothelial markers in plasma could help to assess endothelial damage. We determined von Willebrand factor (measured as Ristocetincofacto...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(95)90867-f
更新日期：1995-01-01 00:00:00
abstract：:We purified the urinary procoagulant from frozen human urine by introducing phenyl-Sepharose hydrophobic chromatography. By this method, the apoprotein of the procoagulant and the lipid-like substance were separately recovered. Upon reassociation with the lipid-like substance or exogenous crude phospholipids, the apop...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(84)90114-2
更新日期：1984-03-15 00:00:00
abstract：:Low circulating protein C (PC) levels have been observed in sepsis, especially in patients with Neisseriae Meningitides infections. Poor clinical outcome and high limb amputation rates have been associated in infected patients with low circulating PC levels. Published studies using activated PC replacement therapy pat...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2014.04.019
更新日期：2014-07-01 00:00:00
abstract：:A new type of assay for heparins has been devised, in which the test material competes with 125I-labelled heparin for binding to protamine-Sepharose. The assay is very sensitive and will measure heparin concentrations down to 10 ng ml-1. It responds to both the degree of sulphation and the molecular weight of acidic p...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(82)90056-1
更新日期：1982-08-15 00:00:00
abstract：:The antithrombotic effect of a synthetic symmetrical triglyceride having eicosapentaenoic acid (EPA) at positions 1 and 3, and gamma-linolenic acid (GLA) at position 2 was investigated. Administration of the triglyceride significantly increased thrombus formation time and thrombotic occlusion time induced by light irr...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(95)99610-k
更新日期：1995-06-01 00:00:00
abstract：INTRODUCTION:Fluid resuscitation after trauma often results in dilutional coagulopathy that may hinder control of bleeding and, once initial hemostasis has been secured, heighten risk of perioperative bleeding when further surgery is required. Since multiple coagulation factor deficiencies typically accompany fluid res...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2009.10.011
更新日期：2010-03-01 00:00:00
abstract：INTRODUCTION:Though thrombin-activatable fibrinolysis inhibitor (TAFI) may contribute to hypercoagulability during pregnancy, limited data are available on the role of TAFI in women with recurrent fetal loss. MATERIAL/METHODS:We performed a case-control study aimed at evaluating any possible association between TAFI l...
journal_title：Thrombosis research
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.thromres.2011.09.029
更新日期：2012-08-01 00:00:00
abstract：:In adult patients with sickle cell disease two distinct subphenotypes have previously been defined: patients with the viscosity-vaso-occlusion subphenotype (VVO) suffer mainly from vaso-occlusive pain crises and have a relatively high hemoglobin concentration. Patients classified as the hemolysis-endothelial dysfuncti...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2013.10.006
更新日期：2013-01-01 00:00:00
abstract：:A prolongation of the thrombin clotting time by at least 25% was found in the presence of 8 of 26 IgG myeloma proteins, 2 of 5 IgA proteins and 1 of 10 macroglobulins, in a concentration of 10 mg/ml. Normal pooled immunoglobulin in concentrations up to 83 mg/ml were inactive. 2 IgG and 2 IgM monoclonal immunoglobulins...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(84)90315-3
更新日期：1984-07-01 00:00:00
