Abstract：
:Von Willebrand Factor is a multimer produced by endothelial cells and megakaryocytes, being stored in intracellular organelles, such as the Weibel-Palade bodies and alpha-granules in endothelial cells and platelets, respectively. This molecule acts as a carrier protein for factor VIIIc, involved in the intrinsic pathway of blood coagulation maintaining its stability in circulation. Von Willebrand Factor also plays an important role in platelet aggregation and adhesion to injured vessel wall. It interacts with platelets through two distinct glycoproteins, GPIb and GPIIb/IIIa. We raised two monoclonal antibodies, ECA-3 and ECA-4, against human umbilical vascular endothelial cells that recognize and immunoprecipitate von Willebrand Factor. Interestingly, ECA-4 monoclonal antibody is able to completely inhibit platelet agglutination induced by ristocetin, suggesting that it binds to von Willebrand Factor close to platelet GPIb binding site. The use of monoclonal antibodies to identify von Willebrand Factor binding regions to factor VIII or platelets has been reported by others. In pulmonary hypertension, abnormalities have been detected on the multimeric structure of the molecule as well as on its proteolytic fragments, by using monoclonal antibodies. Moreover, monoclonal antibodies raised against specific regions of von Willebrand Factor molecule may allow studies of functional abnormalities of this protein in inherited and acquired disorders like subtypes of von Willebrand's disease.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Spadafora-Ferreira M,Lopes AA,Coelho V,Guilherme L,Kalil Jdoi
10.1016/s0049-3848(99)00122-xsubject
Has Abstractpub_date
2000-01-15 00:00:00pages
3-13issue
2eissn
0049-3848issn
1879-2472pii
S0049-3848(99)00122-Xjournal_volume
97pub_type
杂志文章abstract：:Stent implantation after balloon dilation of coronary arteries has improved clinical prognosis in patients undergoing transluminal coronary angioplasty (PTCA), but late restenosis remains a relevant problem. A previous study has indicated that PAI-1 activity changes immediately after PTCA without stent implantation ar...
journal_title：Thrombosis research
pub_type： 临床试验,杂志文章
doi：10.1016/s0049-3848(01)00360-7
更新日期：2001-11-01 00:00:00
abstract：:Platelet aggregation in platelet-rich plasma (PRP) is more sensitive to agonists and more resistant to antagonists than that in whole blood. In this study, we show that the presence of neutrophils in whole blood accounts at least in part for diminished platelet aggregation in whole blood. The platelet-inhibitory effec...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(90)90216-y
更新日期：1990-12-01 00:00:00
abstract：:Von Willebrand disease (VWD) is classified into three primary subtypes; type 1 includes partial quantitative deficiency, type 2 (A, B, M and N) includes qualitative defects and type 3 includes virtually complete deficiency of von Willebrand factor (VWF). Type 2A includes variants with decreased platelet adhesion cause...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2007.03.014
更新日期：2007-01-01 00:00:00
abstract：:Hip joint replacement surgery, using acrylic cement for prosthesis fixation, is associated with intraoperative cardiorespiratory dysfunction, and a high frequency of postoperative proximal deep vein thrombosis (DVT). Levels of prothrombin fragments 1+2 (F1+2), tissue plasminogen activator antigen (t-PA), plasminogen a...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(93)90087-5
更新日期：1993-06-15 00:00:00
abstract：:Both venous thromboembolism and intracranial metastases are common complications in the setting of primary brain tumors and metastatic malignancies. Anticoagulation is indicated in the presence of cancer-associated thrombosis in order to limit the risk of pulmonary embolism; however, there is reluctance to initiate an...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/S0049-3848(16)30100-1
更新日期：2016-04-01 00:00:00
abstract：:RS-5186, sodium 6-[2-[1-(1H)-imidazolyl]methyl-4,5-dihydrobenzo[b]thiophene]- carboxylate, inhibited platelet thromboxane A2 (TXA2) synthetase with IC50 values of 6 nM and 13 nM for human and rabbit microsomes, respectively. It had a selectivity for TXA2 synthetase 10(5)-fold greater than that for cyclooxygenase, PGI2...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(88)90116-8
更新日期：1988-09-01 00:00:00
abstract：:A variant of type II von Willebrand disease (vWd) is described in a young woman and her mother with severe lifelong bleeding histories. On electrophoresis with low-resolution agarose gels the plasma of the proband lacked large and intermediate-size multimers of von Willebrand factor (vWF) but the platelet multimeric s...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(92)90165-7
更新日期：1992-02-01 00:00:00
abstract：INTRODUCTION:Inter-individual variability in clopidogrel responsiveness is dependent on genetic polymorphisms. We aimed to investigate the impact of 3 genetic polymorphisms involved in clopidogrel metabolism on a strategy of dose-adjustment according to platelet reactivity (PR) monitoring. MATERIAL AND METHODS:[correc...
journal_title：Thrombosis research
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1016/j.thromres.2011.12.038
更新日期：2012-07-01 00:00:00
abstract：:Blackfoot disease is a unique endemic and chronic progressive arteriosclerotic vascular disease in southwest area of Taiwan. In this study, we determined the plasma levels of tissue plasminogen activator (tPA), plasminogen activator inhibitor (PAI), urokinase plasminogen activator (uPA), and von Willebrand factor (vWF...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(93)90188-t
更新日期：1993-11-01 00:00:00
abstract：:Human platelets contain a single membrane glycoprotein which is susceptible to thrombin proteolysis, glycoprotein V. We have purified 1 mg of glycoprotein V from 10(13) platelets using a combination of gel filtration, hydroxylapatite and ion-exchange chromatographies. Glycoprotein V has a blocked amino-terminus. Follo...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(89)90113-8
更新日期：1989-01-01 00:00:00
abstract：:Activation of washed platelets in the presence of EDTA with either 1 U/ml of alpha-thrombin or 2 microM calcium ionophore (A23187) caused the release of one-third to one-half of the platelet factor VIII/von Willebrand factor (FVIII/vWF) into the supernatant. When calcium was present in excess, only 10% of the platelet...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(84)90282-2
更新日期：1984-09-01 00:00:00
abstract：:Three commercial thrombin preparations used clinically to stop topical bleeding were studied. The specific activities of these preparations were 1,064 U/mg (human); 59 U/mg (bovine) and 147 U/mg (bovine). SDS-PAGE analysis of the human product produced one major band corresponding to a molecular weight of alpha-thromb...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(89)90102-3
更新日期：1989-02-01 00:00:00
abstract：:Adenosine is an endogenous antiaggregating substance that influences the platelet responses through specific A-type receptors that activate adenylate cyclase increasing the levels of 3',5'-cyclic adenosine monophosphate (cAMP). In this study, we investigated whether adenosine can also influence the levels of 3',5'-cyc...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(01)00412-1
更新日期：2002-01-01 00:00:00
abstract：BACKGROUND:The treatment of venous thromboembolism (VTE) in cancer patients remains controversial. PURPOSE:The Italian Society for Thrombosis and Haemostasis (SISET) commissioned a project to develop clinical practice guidelines for the therapy of VTE in patients with malignancies. METHODS:Key questions about the tre...
journal_title：Thrombosis research
pub_type： 杂志文章,实务指引
doi：10.1016/j.thromres.2009.08.006
更新日期：2009-11-01 00:00:00
abstract：:Several single-nucleotide polymorphisms (SNPs) have been recognized as associated with ischemic heart disease (IHD) although the optimal set of risk genotypes has not be identified. This study aimed to examine whether identified high-risk SNPs are associated with early onset of IHD. In the GENOCOR study, 44 high-risk ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2014.03.006
更新日期：2014-05-01 00:00:00
abstract：:Severe rejection crises occurred in 13 out of 20 cadaver kidney recipients; removal of the graft was inevitable in 10 cases. The plasminogen levels were found to be lower (76.5 +/- 2.9%) in the 13 patients with impaired graft acceptance (group B) than in the 7 patients experiencing only moderate rejection (group A, 93...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(86)90257-4
更新日期：1986-05-01 00:00:00
abstract：:The proteinase, brinase (Mr approximately 35000), from Aspergillus oryzae, which has been used in therapeutic attempts as a thrombolytic agent in arterial thrombosis, binds to purified human alpha 2-macroglobulin (alpha 2M) with a stoichiometry of 1.7-1.9 mol of enzyme/mol inhibitor. This binding leads to quantitative...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(88)90359-3
更新日期：1988-01-01 00:00:00
abstract：:It has been found in our laboratory that compression induces disaggregation and decompression induces aggregation of human platelets. The experimental design was based on the premise that oppositely charged amino acid residues of fibrinogen are juxtaposed in the specific adhesive site(s). This can be explained by the ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(87)90083-1
更新日期：1987-03-15 00:00:00
abstract：:Deep vein thrombosis and pulmonary embolism, collectively termed venous thromboembolism (VTE), affect over 1 million Americans each year. VTE is triggered by inflammation and blood stasis leading to the formation of thrombi rich in fibrin and red blood cells (RBCs). However, little is known about mechanisms regulating...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2014.03.017
更新日期：2014-05-01 00:00:00
abstract：:Antithrombin III (AT III) is increased in situations where there is increased platelet turnover. Plasma AT III levels measured in 39 renal transplant recipients were significantly higher than in 20 healthy subjects (p less than 0.001) and 20 patient controls (p less than 0.025). AT III levels were significantly correl...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(85)90147-1
更新日期：1985-05-01 00:00:00
abstract：BACKGROUND:Non-vitamin K antagonist oral anticoagulants (NOACs) do not need routine laboratory monitoring but measurement of drug concentration is important in emergency conditions. Specific laboratory tests are not readily available or not implemented in every hospital. Point-of-Care Tests (POCT) may bridge this gap a...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2018.01.044
更新日期：2018-03-01 00:00:00
abstract：INTRODUCTION:Adenosine diphosphate (ADP) as physiological activator of human platelets mediates its effects via three purinergic receptors: P2Y1, P2Y12 and P2X1. The inhibition of P2Y12 is used pharmacologically to suppress aggregation underlining the physiological significance of this receptor. Since the regulation of...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2018.05.029
更新日期：2018-08-01 00:00:00
abstract：:To estimate the effect of dilazep in preventing restenosis after a transluminal angioplasty, we have attempted an animal experiment in which the efficacy of drug was tested on an angioplastic injury superimposed on to atherosclerotic lesions. Using a modification of Block's method, atherosclerotic lesions first were m...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(89)90122-9
更新日期：1989-06-15 00:00:00
abstract：INTRODUCTION:Pelvic deep vein thrombosis (DVT) is difficult to diagnose during pregnancy. In a two-center trial, we evaluated the agreement between ultrasonography and magnetic resonance imaging (MRI) in diagnosing the extent of DVT into the pelvic veins during pregnancy. MATERIALS AND METHODS:Pregnant women with prox...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2010.05.011
更新日期：2010-08-01 00:00:00
abstract：:Chronic thromboembolic pulmonary hypertension (CTEPH) is the most severe long term complication of acute pulmonary embolism (PE). Untreated, CTEPH is associated with a very poor prognosis and high risk of mortality, although curation can be achieved by surgical removal of the obstructive endothelialised thromboemboli ...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2016.12.026
更新日期：2017-03-01 00:00:00
abstract：:Triflavin, an Arg-Gly-Asp (RGD)-containing disintegrin purified from venom peptide inhibited platelet aggregation by interfering with the interaction of fibrinogen with alpha(IIb)beta(3) integrin. Using an immunostaining technique and electron microscopy, we investigated and compared the distribution of triflavin bind...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(03)00104-x
更新日期：2003-01-01 00:00:00
abstract：:Defibrotide (D) is a natural polydeoxyribonucleotide from mammalian lungs with profibrinolytic and antithrombotic activities. D also has PGI2-stimulating and tissue plasminogen activator (TPA)-releasing activities, but has no anticoagulant properties. The protective effects of D were demonstrated very recently in a mo...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(86)90265-3
更新日期：1986-05-01 00:00:00
abstract：INTRODUCTION:Thrombomodulin (TM) has been described as a marker of endothelial injury in atherosclerosis. The role of TM as a predictor of PAD severity is to be proven. The goal of the present study is to compare the level of plasmatic (TMp) in patients with intermittent claudication with patients with critical ischemi...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2005.03.010
更新日期：2006-01-01 00:00:00
abstract：:The effect of suloctidil (600 mg/day) on platelet survival time (PST) and plasma and urine betathromboglobulin (BTG) was studied in a double-blind, placebo-controlled six-week crossover trial in 13 patients with shortened PST (less than 110 hrs, exponential model). Mean PST after suloctidil (110.6 hrs) was significant...
journal_title：Thrombosis research
pub_type： 临床试验,杂志文章
doi：10.1016/0049-3848(84)90231-7
更新日期：1984-08-15 00:00:00
abstract：BACKGROUND:The usefulness of coagulation tests performed before epidural analgesia for surgery or to alleviate labour pain is controversial. The aims of this study were: (1) to evaluate the prevalence of abnormal tests in a large cohort of healthy pregnant women and their association with epidural hematoma; (2) to asse...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2011.02.023
更新日期：2011-07-01 00:00:00
