Abstract：
:Activation of washed platelets in the presence of EDTA with either 1 U/ml of alpha-thrombin or 2 microM calcium ionophore (A23187) caused the release of one-third to one-half of the platelet factor VIII/von Willebrand factor (FVIII/vWF) into the supernatant. When calcium was present in excess, only 10% of the platelet FVIII/vWF was detected free in the supernatant, regardless of whether calcium was present before stimulation or added to the platelets after thrombin activation. Release of [14C]serotonin and beta-thromboglobulin were not affected by divalent cations indicating that reduced supernatant levels of FVIII/vWF in the presence of calcium were not due to differential release, but were probably due to a calcium-dependent association of released FVIII/vWF with the platelet surface. The presence or absence of intact glycoprotein Ib on the platelet surface made no significant difference to the observed FVIII/vWF partition. Platelets from a patient with Glanzmann's thrombasthenia, however, failed to show a calcium effect with respect to released FVIII/vWF. The combined results suggest that as well as the ristocetin-dependent, divalent cation-independent binding of FVIII/vWF to glycoprotein Ib, there is a divalent cation-dependent binding of FVIII/vWF to the activated platelet surface which is mediated via the glycoprotein IIb/IIIa complex.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Furby FH,Berndt MC,Castaldi PA,Koutts Jdoi
10.1016/0049-3848(84)90282-2subject
Has Abstractpub_date
1984-09-01 00:00:00pages
501-11issue
5eissn
0049-3848issn
1879-2472pii
0049-3848(84)90282-2journal_volume
35pub_type
杂志文章abstract：BACKGROUND:Although patients undergoing coronary stenting routinely receive dual antiplatelet treatment to reduce the risk of stent thrombosis, this undesired event still occurs. A suboptimal response to clopidogrel treatment (low responders) has been suggested to contribute to stent thrombosis. In the present study, p...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2004.07.007
更新日期：2005-01-01 00:00:00
abstract：:Initiation of clotting cascade by tissue factor (TF): Factor VII (FVII) complex is essential for hemostasis however pathologic expression of TF leads to thrombosis. In contrast, the contact pathway factor XII, and to the smaller extent FXI are dispensable for normal hemostasis but still contribute to pathologic thromb...
journal_title：Thrombosis research
pub_type：
doi：10.1016/j.thromres.2014.03.001
更新日期：2014-05-01 00:00:00
abstract：INTRODUCTION:Circulating microparticles support thrombin generation. The aim of this study is to determine the indirect microparticle activity and the parameters of thrombin generation in healthy infants. MATERIALS AND METHODS:A total of 85 infants who were brought to follow-up visits were taken into the study. Blood ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2013.11.011
更新日期：2014-02-01 00:00:00
abstract：INTRODUCTION:The Coasys® Plus C (Behnk Elektronik, distributed by Roche Diagnostics) is a coagulation analyzer for small to midsize clinical chemistry laboratories. We performed a laboratory evaluation. MATERIALS AND METHODS:After a familiarization period the dead volume, carry-over, capacity, within-assay reproducibi...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2013.02.004
更新日期：2013-04-01 00:00:00
abstract：:Alterations in the synthesis or enhanced inactivation of nitric oxide (NO) and an increase in endothelin-1 production lead to an imbalance that can induce arterial hypertension. Type II diabetes is characterized by impaired endothelium-dependent vasodilation and vascular disease. NO is produced through L-arginine path...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(01)00237-7
更新日期：2001-04-15 00:00:00
abstract：INTRODUCTION:Peden et al. have revealed a significant association between four new risk loci and coronary heart disease (CHD) in Europeans and South Asians. The goal of this study is to evaluate the contribution of these genetic loci to CHD risk in Han Chinese. METHODS:We recruited 161 CHD patients and 112 controls pr...
journal_title：Thrombosis research
pub_type： 杂志文章,meta分析
doi：10.1016/j.thromres.2012.05.023
更新日期：2012-10-01 00:00:00
abstract：INTRODUCTION:Animal models of thrombosis and hemostasis are critical for target validation in pharmaceutical research. Guinea pig haemostatic mechanisms, such as the platelet thrombin receptor repertoire, resemble those of humans. Measuring the performance characteristics of marketed antithrombotic drugs in guinea pig ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2008.03.010
更新日期：2008-01-01 00:00:00
abstract：INTRODUCTION:To validate the diagnostic criteria for disseminated intravascular coagulation (DIC) established by the Japanese Association for Acute Medicine (JAAM) at an early stage of trauma and to evaluate the hypothesis that the JAAM criteria can diagnose DIC with a higher sensitivity than the International Society ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2009.06.036
更新日期：2009-12-01 00:00:00
abstract：:Catharsius protease-1 (CPM-1) was isolated from the whole body of the dung beetles, Catharsius molossus, using three purification steps (ammonium sulfate fractionation, gel filtration on Bio-Gel P-60, and affinity chromatography on DEAE Affi-Gel Blue gel). The purified CPM-1 that has a molecular weight of 27 kDa was a...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2004.01.005
更新日期：2003-01-01 00:00:00
abstract：:We provide a centralised thrombophilia screening service with sample transfer by courier or first class mail, a practice common to many centres. Sample quality is of prime importance, thus we have assessed the effect of delayed sample handling upon the haemostatic variables within our thrombophilia profile. This compr...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(97)00187-4
更新日期：1997-09-15 00:00:00
abstract：:Venous thromboembolism (VTE) is a preventable disease, yet it is one of the leading causes of death among patients with cancer. Improving risk stratification mechanisms will allow us to personalize thrombo-prophylaxis strategies. We sought to evaluate Collagen and Thrombin Activated Platelets (COAT-platelets) as well ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2015.10.004
更新日期：2015-12-01 00:00:00
abstract：:Liquid permeation studies of fibrin gels, which were formed in the presence of thrombin (Fibrin II) and in the presence of Batroxobin (Fibrin I), showed that those gels have distinctly different flow properties. For both gels the permeability coefficient (Ks) and average pore size varied depending on the conditions fo...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(82)90214-6
更新日期：1982-01-01 00:00:00
abstract：:Capsaicin was found to be a potent inhibitor of platelet aggregation and release reaction. It inhibited the aggregation of rat platelet induced by collagen and thrombin, but only slightly reduced those of AA and A23187. The IC50 on collagen-induced platelet aggregation was about 85 micrograms/ml. Less inhibition was o...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(84)90189-0
更新日期：1984-12-15 00:00:00
abstract：:We have investigated fibrinolytic parameters in 33 patients with peripheral arterial occlusive disease (PAOD) before and 6, 24, and 48 hours after percutaneous transluminal angioplasty (PTA) as well as in 35 gender-matched healthy controls, whose mean age was not significantly different from the mean age of the patien...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(99)00002-x
更新日期：1999-05-15 00:00:00
abstract：INTRODUCTION:Diabetes mellitus is characterised by hyperglycaemia that plays an important role in the pathogenesis of diabetic complications including accumulation of methylglyoxal (MG), a highly reactive alpha-dicarbonyl metabolite of glucose degradation pathways and increased generation of advanced glycation end prod...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2007.03.002
更新日期：2007-01-01 00:00:00
abstract：:The inhibitory effects of several antiinflammatory agents on the specific binding of tritiated 1-O-alkyl-2-O-acetyl-sn-glycero-3-phosphorylcholine, (platelet activating factor, PAF), with its receptor on isolated rabbit platelet plasma membranes were investigated. Several potent cyclooxygenase inhibitors do not inhibi...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(84)90256-1
更新日期：1984-06-15 00:00:00
abstract：INTRODUCTION:Many antithrombotic drugs may have a deleterious effect on normal haemostasis leading to bleeding complications. The aim of this study was to determine if sub-therapeutic (low) doses of antithrombotic agents, when administered in combination, have enhanced efficacy without augmentation of bleeding time. M...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2005.11.015
更新日期：2006-01-01 00:00:00
abstract：BACKGROUND:Peripheral pulmonary artery thrombus (PPT) is common in the clinic. However, due to the lack of an ideal diagnostic tool, PPT cannot be quickly diagnosed and effectively treated at present. Optical coherence tomography (OCT) is a new intravascular imaging technique that is characterized by high image resolut...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2017.10.021
更新日期：2018-01-01 00:00:00
abstract：INTRODUCTION:Endothelium-derived nitric oxide (NO) is synthesized from l-arginine by endothelial nitric oxide synthase (eNOS) encoded by the eNOS3 gene on chromosome 7. The effects of the eNOS polymorphisms with the risk of coronary artery disease are conflicting. In this study, we investigated the association of the e...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2006.06.005
更新日期：2007-01-01 00:00:00
abstract：:We investigated the age-related changes in blood coagulation, fibrinolysis, and platelet aggregation in male WBN/Kob rats, animals that exhibit spontaneously diabetes mellitus at more than 6 months of age. The rats aged 6 months or more showed significant hyperglycemia, hypoinsulinemia, and hyperlipidemia. As changes ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(00)00196-1
更新日期：2000-06-15 00:00:00
abstract：BACKGROUND:Although excess platelet activation, as indicated by increased plasma beta thromboglobulin (beta-TG), has been shown in pregnancy-induced hypertension (PIH), platelet adhesion, platelet morphology and a comparison of platelet and soluble (plasma) levels of the adhesion molecules P-selectin (pPsel and sPsel, ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2005.01.009
更新日期：2005-01-01 00:00:00
abstract：:Human platelets synthesize nitric oxide (NO) through an endothelial-type NO synthase (ecNOS) activated also by substances enhancing 3',5'-cyclic adenosine monophosphate (cAMP) concentrations, such as catecholamines, beta-adrenoceptor agonists and adenosine. To verify whether cAMP directly activates ecNOS through the c...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2004.06.036
更新日期：2004-01-01 00:00:00
abstract：INTRODUCTION:Recombinant members of the vitamin K-dependent protein family (factors IX and VII and protein C) have become important pharmaceuticals in treatment of bleeding disorders and sepsis. However, because the in vivo gamma-carboxylation system in stable cell lines used for transfection has a limited capacity of ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2007.11.002
更新日期：2008-01-01 00:00:00
abstract：:Practice variation, the differences in clinical management between physicians, is one reason why patient outcomes may differ. Identifying factors that contribute to practice variation in areas of clinical uncertainty or equipoise may have implications for understanding and improving patient care. To discern what facto...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2017.09.026
更新日期：2017-11-01 00:00:00
abstract：INTRODUCTION:Microparticles (MPs) are small fragments of apoptotic or activated cells that may contribute to pathological processes in cardiovascular diseases. In studies of MPs in clinical cohorts, it is unclear if observed changes in MP composition are a cause or a result of the cardiovascular disease being studied. ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2011.09.016
更新日期：2012-07-01 00:00:00
abstract：INTRODUCTION:Cancer associated thrombosis is a well-recognized phenomenon that results in considerable patient morbidity and mortality. Malignancy conveys an increased risk for thrombosis and chemotherapy further elevates this risk. The pathophysiological mechanisms underlying this process remain poorly defined. MATER...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2011.06.022
更新日期：2012-02-01 00:00:00
abstract：:Surviving an embolism exposes patients to potential long-term complications, such as altered quality of life, persistent dyspnea, impaired exercise capacity or pulmonary hypertension. The common objective factor in most of these situations is the presence of residual pulmonary vascular obstruction (RPVO). Planar venti...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2019.09.038
更新日期：2019-12-01 00:00:00
abstract：:Annexin V is a human phospholipid binding protein (M(r) 36,000) that binds with high affinity to activated platelets in vitro. We studied the biodistribution and thrombus binding of annexin V in rabbit and swine models of fully occlusive arterial thrombi formed 1-2 h prior to injection of annexin V. Iodinated annexin ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(94)90224-0
更新日期：1994-09-01 00:00:00
abstract：:It is known that a low-molecular-weight heparin (LMWH) is more effective than unfractionated heparin in unstable angina/non-Q-wave myocardial infarction (UA/NQMI) and the platelet GPIIb/IIIa receptors play an important role in acute myocardial infarction (AMI). Therefore, enoxaparin might have a similar advantage over...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(01)00242-0
更新日期：2001-05-01 00:00:00
abstract：:An issue of great importance and continuing concern with regard to all products derived from human plasma is their safety from potential contaminants in the source material from which they are purified. Since viral contaminants are a major safety consideration with these products, a number of different methods, includ...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/s0049-3848(02)00044-0
更新日期：2002-03-01 00:00:00
