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Platelet membrane glycoprotein V: characterization of the thrombin-sensitive glycoprotein from human platelets.

Abstract：

:Human platelets contain a single membrane glycoprotein which is susceptible to thrombin proteolysis, glycoprotein V. We have purified 1 mg of glycoprotein V from 10(13) platelets using a combination of gel filtration, hydroxylapatite and ion-exchange chromatographies. Glycoprotein V has a blocked amino-terminus. Following proteolysis by human alpha-thrombin, a major fragment, termed glycoprotein Vf1, had the sequence Gly-Pro-Phe-X-Arg-Pro-Ala-Ala-Asp-Glu-Ser-Val-Glu-Ala-Pro-Val-Asn-Gln-Al a-Glu- Ala-Pro-. The purified glycoprotein was not a substrate for human gamma-thrombin. Glycoprotein V contained 17.5% carbohydrate, with the majority of the carbohydrate consisting of neutral hexoses. Deglycosylated glycoprotein V had a molecular weight of 57.5 kDa compared to the glycosylated protein's 82 kDa and the deglycosylated protein was recognized by polyclonal antibodies raised against glycoprotein V. Immunoelectrophoresis of human and rat platelets and megakaryocytes gave a single immunoreactive band, with the rat glycoprotein having a slightly larger molecular mass. Glycoprotein V is most likely an integral membrane protein.

journal_name

Thromb Res

journal_title

Thrombosis research

authors

Zafar RS,Walz DA

doi

10.1016/0049-3848(89)90113-8

subject

Has Abstract

pub_date

1989-01-01 00:00:00

pages

31-44

issue

1

eissn

0049-3848

issn

1879-2472

pii

0049-3848(89)90113-8

journal_volume

53

pub_type

杂志文章

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