当前位置： SCI文献检索 > THROMBOSIS RESEARCH期刊下所有文献 > Assembly of the prothrombinase complex on the surface of human foreskin fibroblasts: Implications for connective tissue growth factor.

Assembly of the prothrombinase complex on the surface of human foreskin fibroblasts: Implications for connective tissue growth factor.

Abstract：

:Activated factor X (FXa) and thrombin can up-regulate gene expression of connective tissue growth factor (CTGF/CCN2) on fibroblasts. Since tissue factor (TF) is expressed on these cells, we hypothesized that they may assemble the prothrombinase complex leading to CTGF/CCN2 upregulation. In addition, the effect of thrombospondin-1 (TSP1) on this reaction was evaluated. Human foreskin fibroblasts were incubated with purified factor VII (FVII), factor X (FX), factor V (FV), prothrombin and calcium in the presence and absence of TSP1. Generation of FXa and of thrombin were assessed using chromogenic substrates. SMAD pathway phosphorylation was detected via Western-blot analysis. Pre-incubation of fibroblasts with FVII led to its auto-activation by cell-surface expressed TF, which in turn in the presence of FX, FVa, prothrombin and calcium led to FXa (9.7±0.8nM) and thrombin (7.9±0.04 U/mL×10-3) generation. Addition of TSP1 significantly enhanced thrombin (23.3±0.7 U/mL×10-3) but not FXa (8.5±0.6nM) generation. FXa and thrombin generation leads to upregulation of CTGF/CCN2. TSP1 alone upregulated CTGF/CCN2, an effect mediated via activation of transforming growth factor beta (TGFβ) as shown by phosphorylation of the SMAD pathway, an event blunted by using a TGFβ receptor I inhibitor (TGFβRI). FXa- and thrombin-induced upregulation of CTGF/CCN2 was not blocked by TGFβRI. In summary, assembly of the prothrombinase complex occurs on fibroblast's surface leading to serine proteases generation, an event enhanced by TSP1 and associated with CTGF/CCN2 upregulation. These mechanisms may play an important role in human diseases associated with fibrosis.

journal_name

Thromb Res

journal_title

Thrombosis research

authors

Rico MC,Rough JJ,Manns JM,Del Carpio-Cano F,Safadi FF,Kunapuli SP,DeLa Cadena RA

doi

10.1016/j.thromres.2011.08.009

subject

Has Abstract

pub_date

2012-06-01 00:00:00

pages

801-6

issue

6

eissn

0049-3848

issn

1879-2472

pii

S0049-3848(11)00431-2

journal_volume

129

pub_type

杂志文章

相关文献

THROMBOSIS RESEARCH文献大全
  • Human and rabbit platelets form platelet-activating factor in response to calcium ionophore.

    abstract：:Homogenous preparations of human and rabbit platelets synthesized platelet-activating factor (PAF) when stimulated by calcium ionophore A23187. PAF was isolated by high performance liquid chromatography (HPLC) on a Lichrosorb Si60 column. PAF was well separated from other phospholipids especially sphingomyelin and lys...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(83)90398-5

    authors： Alam I,Smith JB,Silver MJ

    更新日期：1983-04-01 00:00:00

  • Two-dimensional electrophoretic studies of platelets from dogs affected with basset hound hereditary thrombopathy: a thrombasthenia-like aggregation defect.

    abstract：:Because of a thrombasthenia-like platelet aggregation defect, platelets from dogs affected with Basset Hound Hereditary Thrombopathy were compared to normal control dog platelets by three different techniques in order to assess platelet membrane glycoprotein content. Crossed immunoelectrophoresis (CIE), two-dimensiona...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(86)90295-1

    authors： Patterson WR,Kunicki TJ,Bell TG

    更新日期：1986-04-15 00:00:00

  • Comparison of four rapid D-Dimer tests for diagnosis of pulmonary embolism.

    abstract：:Aim of this study was to evaluate rapid D-Dimer tests for their utility in diagnosis of acute pulmonary embolism (PE). Tests were performed in 183 consecutive pats referred for lung scanning because of clinically suspected PE. According to lung scans and the clinical course of disease 19 pats were classified to have P...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(96)00089-8

    authors： Veitl M,Hamwi A,Kurtaran A,Virgolini I,Vukovich T

    更新日期：1996-06-01 00:00:00

  • Estimation of EDRF and nitric oxide release using [3H]GTP-labeled human platelets.

    abstract：:We have developed a new bioassay for endothelium-derived relaxing factor (EDRF) or nitric oxide (NO) using human [3H]guanosine triphosphate (GTP)-labeled platelets. The labeled platelets were preincubated with isobutyl-methylxanthine and co-cultured with endothelial cells and the [3H]cyclic guanosine monophosphate (cG...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(95)00082-3

    authors： Kishi Y,Watanabe R,Ashikaga T,Numano F

    更新日期：1995-06-15 00:00:00

  • Sequential intrapulmonary and systemic activation of coagulation and fibrinolysis during and after total hip replacement surgery.

    abstract：:Hip joint replacement surgery, using acrylic cement for prosthesis fixation, is associated with intraoperative cardiorespiratory dysfunction, and a high frequency of postoperative proximal deep vein thrombosis (DVT). Levels of prothrombin fragments 1+2 (F1+2), tissue plasminogen activator antigen (t-PA), plasminogen a...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(93)90087-5

    authors： Dahl OE,Pedersen T,Kierulf P,Westvik AB,Lund P,Arnesen H,Seljeflot I,Abdelnoor M,Lyberg T

    更新日期：1993-06-15 00:00:00

  • Effect of a collagen derived octapeptide on different steps of the platelet/collagen interaction.

    abstract：:The interaction of platelets with collagen involves short aminoacid sequences which recur along the fibres. Platelet aggregation by collagen and serotonin release is inhibited by a synthetic octapeptide LYS-PRO-GLY-GLU- PRO-GLY-PRO-LYS- derived from type III collagen. In contrast, this octapeptide inhibits only weakly...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(83)90061-0

    authors： Karniguian A,Legrand YJ,Lefrancier P,Caen JP

    更新日期：1983-12-15 00:00:00

  • The continuous infusion of recombinant activated factor VIIa (rFVIIa) in patients with factor VIII inhibitors activates the coagulation and fibrinolytic systems without clinical complications.

    abstract：:The standard modality of administration of rFVIIa to patients with FVIII and FIX inhibitors is the intermittent infusion every 2 to 6 hours. No untoward local or systemic effects have been reported; laboratory data of activation of coagulation were reported in the presence of coexistent problems (sepsis, septic shock)...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/s0049-3848(00)00220-6

    authors： Baudo F,Redaelli R,Caimi TM,Mostarda G,Somaini G,de Cataldo F

    更新日期：2000-07-01 00:00:00

  • Reversible acylation of factor Xa as a potential therapy for hemophilia.

    abstract：:Current therapies for treatment of hemophilia A involve infusion of factor VIII, but are ineffective for patients who develop inhibitory antibodies. We have previously proposed that bypassing the intrinsic pathway (VIIIa/IXa) with reversibly acylated factor Xa offers an improvement on existing therapies as it provides...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/s0049-3848(97)00267-3

    authors： Lin PH,Laibelman AM,Sinha U

    更新日期：1997-11-15 00:00:00

  • Knockdown of liver-derived factor XII by GalNAc-siRNA ALN-F12 prevents thrombosis in mice without impacting hemostatic function.

    abstract：BACKGROUND:Plasma coagulation Factor XII (FXII) plays a crucial role in contact activation, ultimately regulating both the kinin-kallikrein system and the intrinsic pathway of coagulation. A growing body of evidence suggests that inhibition of FXII can prevent thrombosis. Given FXII does not appear to modulate hemostas...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2020.08.040

    authors： Liu J,Cooley BC,Akinc A,Butler J,Borodovsky A

    更新日期：2020-12-01 00:00:00

  • Efficacy and safety of prolonged thromboprophylaxis with a low molecular weight heparin (dalteparin) after total hip arthroplasty--the Danish Prolonged Prophylaxis (DaPP) Study.

    abstract：:The aim of this study was to compare the efficacy and safety of prolonged (35 days) thromboprophylaxis with a standard length (7 days) regimen of a low molecular weight heparin in patients undergoing total hip arthroplasty. The study was multicentre, randomised, double-blind, and prospective with two groups. Following...

    journal_title：Thrombosis research

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1016/s0049-3848(98)00018-8

    authors： Lassen MR,Borris LC,Anderson BS,Jensen HP,Skejø Bro HP,Andersen G,Petersen AO,Siem P,Hørlyck E,Jensen BV,Thomsen PB,Hansen BR,Erin-Madsen J,Møller JC,Rotwitt L,Christensen F,Nielsen JB,Jørgensen PS,Paaske B,Tørholm

    更新日期：1998-03-15 00:00:00

  • Studies on the anticoagulant action of Aspilia africana.

    abstract：:An anticoagulant activity was identified and isolated from the leaves of a West African plant, Aspilia africana by gel filtration on Sephadex G-100. The anticoagulant factor had an apparent molecular weight of approximately 60,000 d. Upon incubation with plasma, it prolonged the partial thromboplastin time, prothrombi...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(87)90455-5

    authors： Hanna MM,Niemetz J

    更新日期：1987-08-15 00:00:00

  • Intensity of anticoagulation and survival in patients hospitalized with COVID-19 pneumonia.

    abstract：BACKGROUND:SARS-CoV-2 infection has noted derangements in coagulation markers along with significant thrombotic complications. Post-mortem examinations show severe endothelial injury and widespread thrombotic microangiopathy in the pulmonary vasculature. Early reports describing the use of prophylactic anticoagulation ...

    journal_title：Thrombosis research

    pub_type： 评论,信件

    doi：10.1016/j.thromres.2020.09.030

    authors： Hsu A,Liu Y,Zayac AS,Olszewski AJ,Reagan JL

    更新日期：2020-12-01 00:00:00

  • D-dimer specific monoclonal antibodies react with fibrinogen aggregates.

    abstract：:Human fibrinogen exposed to 46.5 degrees C was subjected to gel permeation chromatography. The protein eluted in two distinct peaks. The first peak appeared in the void volume containing soluble fibrinogen aggregates, while the other peak represented monomeric fibrinogen. In contrast to the monomeric peak material, th...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(96)00063-1

    authors： Bennick A,Haddeland U,Brosstad F

    更新日期：1996-04-15 00:00:00

  • No firm association between N-terminal pro-brain natriuretic peptide and percentage of pulmonary vascular obstruction in patients with acute pulmonary embolism.

    abstract：INTRODUCTION:High concentrations of N-terminal-pro-brain natriuretic peptide (NT-proBNP) are found in patients with right ventricular overload. Right ventricular overload may be the result of large perfusion defects in patients with pulmonary embolism (PE). NT-proBNP levels are associated with poorer outcome in patient...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2011.02.014

    authors： Agterof MJ,Schutgens RE,Verzijlbergen JF,van Buul MM,Tromp EA,Eijkemans MJ,van der Griend R,Biesma DH

    更新日期：2011-06-01 00:00:00

  • Genetic and molecular evidence for complement dysregulation in patients with HELLP syndrome.

    abstract：BACKGROUND:HELLP (Hemolysis, elevated liver enzymes and low platelets) syndrome is a severe and acute pregnancy-related disorder that occurs in approximately 2.5 per 1000 deliveries and represents a major cause of maternal and perinatal morbidity and mortality. This syndrome has been suggested to be a microangiopathy a...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2020.08.038

    authors： Bazzan M,Todros T,Tedeschi S,Ardissino G,Cardaropoli S,Stella S,Montaruli B,Marchese C,Roccatello D,Cugno M

    更新日期：2020-12-01 00:00:00

  • "A" subunit of factor XIII is present on bovine platelet membrane and mediates collagen-induced platelet activation.

    abstract：:The Fab fragment of a polyclonal antibody against platelet factor XIII inhibited the collagen-induced platelet aggregation in a dose-dependent manner. This inhibitory effect was specific for collagen, and it had no effect on arachidonic acid-, ADP-, and serotonin-induced aggregations. This finding strengthens our noti...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(88)90226-5

    authors： Kasahara K,Takagi J,Sekiya F,Inada Y,Saito Y

    更新日期：1988-04-15 00:00:00

  • Formation of the clot.

    abstract：:An electron transport system regulates the initiation of thrombus formation through the activation of critical receptors involved in hemostasis and thrombosis. Protein disulfide isomerase along with other thiol isomerases, important for intracellular protein synthesis, are responsible for this extracellular activity d...

    journal_title：Thrombosis research

    pub_type： 杂志文章,评审

    doi：10.1016/j.thromres.2012.08.272

    authors： Furie B,Furie BC

    更新日期：2012-10-01 00:00:00

  • Excessive expressions of T cell activation markers in pediatric immune thrombocytopenia.

    abstract：BACKGROUND:Immune thrombocytopenia (ITP) is an immune-mediated bleeding disorder in children. Activated T cells have been shown to play important roles in ITP. The aims of this study were to evaluate whether these T cell activation markers could be used as indicators to differentiate ITP patients from controls, and to ...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2019.05.010

    authors： Chen Y,Zhou Y,Chen P,Zhang P,Jia M,Tang Y

    更新日期：2019-08-01 00:00:00

  • Combination and comparison of two models in prognosis of pulmonary embolism: results from TUrkey Pulmonary Embolism Group (TUPEG) study.

    abstract：BACKGROUND:Clinical parameters, biomarkers and imaging-based risk stratification are widely accepted in pulmonary embolism(PE). The present study has investigated the prognostic role of simplified Pulmonary Embolism Severity Index (sPESI) score and the European Society of Cardiology (ESC) model. METHODS:This prospecti...

    journal_title：Thrombosis research

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.thromres.2014.02.032

    authors： Ozsu S,Ozlu T,Sentürk A,Uçar EY,Kırkıl G,Kadıoğlu EE,Altınsoy B,Saylan B,Selimoğlu HŞ,Dabak G,Tutar N,Uysal A,TUPEG Study Investigators.

    更新日期：2014-06-01 00:00:00

  • The synthetic pentasaccharide SR 90107A/Org 31540 does not release lipase activity into the plasma.

    abstract：:The present study was designed to find out whether the synthetic pentasaccharide SR 90107A/Org 31540, which is presently being evaluated in clinical trials as an antithrombotic agent, influences lipoprotein metabolism in rats as determined by plasma triglyceride (TG) lipase activity. A comparison with three clinically...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/s0049-3848(97)00075-3

    authors： Hoffmann P,Bernat A,Dumas A,Petitou M,Hérault JP,Herbert JM

    更新日期：1997-05-15 00:00:00

  • Substitution of antithrombin III in shock and DIC: a randomized study.

    abstract：:In 51 shock patients with DIC Antithrombin III (AT III) substitution, heparin or a combination of both substances respectively was administered. In the two groups which had been given AT III substitution the concentration of AT III rose considerably higher than the activity. There was a drop of the platelet count in b...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(85)90123-9

    authors： Blauhut B,Kramar H,Vinazzer H,Bergmann H

    更新日期：1985-07-01 00:00:00

  • Fluorescent labeling of blood platelets in vivo.

    abstract：:In rabbits, a clear microscopic image of individual, fluorescent blood platelets flowing in a mesenteric microvessel could be obtained over the whole cross-sectional area of the vessel, following an intravenous injection of a solution containing 5-15 mg of the fluorochrome Acridine Red. Most of the circulating platele...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(82)90106-2

    authors： Tangelder GJ,Slaaf DW,Reneman RS

    更新日期：1982-12-15 00:00:00

  • Clustering of venous thrombosis events at the start of tamoxifen therapy in breast cancer: a population-based experience.

    abstract：INTRODUCTION:The epidemiology of tamoxifen and venous thromboembolism (VTE) is not well understood, and most data on tamoxifen toxicity are from adjuvant clinical trials. This study examined the relationship between the duration of tamoxifen use in female patients with breast cancer and the risk of VTE in a large popul...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2011.11.025

    authors： Onitilo AA,Doi SA,Engel JM,Glurich I,Johnson J,Berg R

    更新日期：2012-07-01 00:00:00

  • Plasma endothelin-1 level as a predictor for poor collaterals in patients with ≥95% coronary chronic occlusion.

    abstract：BACKGROUND:Coronary collateral circulation (CCC) plays an important role in protecting myocardium from ischemic damage. The studies on factors which impact on CCC might be of great clinical interest. The aim of the present study was to evaluate endothelin-1 (ET-1) as a potential predictor for poor or good CCC in patien...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2016.04.007

    authors： Fan Y,Li S,Li XL,Lin XL,Zhu CG,Xu RX,Qing P,Wu NQ,Jiang LX,Xu B,Gao RL,Li JJ

    更新日期：2016-06-01 00:00:00

  • Recurrent thrombosis in antiphospholipid syndrome may be associated with cardiovascular risk factors and inflammatory response.

    abstract：INTRODUCTION:Antiphospholipid syndrome (APS) is a pro-thrombotic autoimmune disease that affects different vascular beds, with potential risk for recurrence. Systemic lupus erythematosus (SLE), specific autoantibodies profile and atherogenic disorders have been described as risk factors for the occurrence of first thro...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2015.10.029

    authors： Saraiva Sda S,Custódio IF,Mazetto Bde M,Collela MP,de Paula EV,Appenzeller S,Annichino-Bizzachi J,Orsi FA

    更新日期：2015-12-01 00:00:00

  • Growth and function of the normal human placenta.

    abstract：:The placenta is the highly specialised organ of pregnancy that supports the normal growth and development of the fetus. Growth and function of the placenta are precisely regulated and coordinated to ensure the exchange of nutrients and waste products between the maternal and fetal circulatory systems operates at maxim...

    journal_title：Thrombosis research

    pub_type： 杂志文章,评审

    doi：10.1016/j.thromres.2004.06.038

    authors： Gude NM,Roberts CT,Kalionis B,King RG

    更新日期：2004-01-01 00:00:00

  • The concomitant augmentation of urokinase-type plasminogen activator and collagenase-like proteinase activities in X-ray irradiated cells of a human metastatic carcinomatous line.

    abstract：:The enzymatic activities of uPA, and a collagenase-like proteinase in the post-nuclear fraction of cell homogenates of a metastatic carcinomatous cell line following X-ray irradiation were examined by the use of chromogenic substrates and by casein- or gelatin-containing zymographies and electrophoretic gel stained wi...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(94)90214-3

    authors： Uchida N,Takahashi K,Kasai T,Ishida T,Kwaan HC

    更新日期：1994-06-15 00:00:00

  • Recombinant human protein C: comparative functional studies with human plasma protein C.

    abstract：:Protein C (PC) is the central protein in a major antithrombotic regulatory mechanism. Hereditary deficiencies of PC are associated with thrombosis. Therapeutic PC replacement may be an important treatment if pure functional human protein C is available in sufficient quantity. Human PC has been produced on a commercial...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(90)90258-e

    authors： Madden RM,Oppenheimer C,Wydro R,Marlar RA

    更新日期：1990-02-01 00:00:00

  • Platelet aggregation monitored in a 96 well microplate reader is useful for evaluation of platelet agonists and antagonists.

    abstract：:Optimal conditions for a method to simultaneously measure aggregation in 96 samples using a microplate reader were developed. The temperature of the assay was set at 25 degrees C, the optimal platelet concentration range was determined to be from 1-3 x 10(8) per mL, the assay volume was determined to be best at 100 mi...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(95)93881-y

    authors： Bednar B,Condra C,Gould RJ,Connolly TM

    更新日期：1995-03-01 00:00:00

  • Glycoprotein IIb-IIIa complex in platelets of patients and heterozygotes of Glanzmann's thrombasthenia.

    abstract：:The concentration of the glycoprotein (GP) IIb-IIIa complex in thrombasthenic platelets of 8 patients of 6 families has been estimated. In the thrombasthenic platelets of 3 patients this complex is absent (thrombasthenia type I and subtype I). In 2 patients only traces are detectable and in 3 patients GP IIb-IIIa comp...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(83)90063-4

    authors： Herrmann FH,Meyer M,Gogstad GO,Solum NO

    更新日期：1983-12-15 00:00:00