Abstract：
BACKGROUND:Nuclear factor kappa B (NF-kappa B) activation and tissue factor (TF) expression may contribute to lethality in sepsis. Inappropriate in vivo expression of TF is likely responsible for fibrin deposition in sepsis-associated disseminated intravascular coagulation (DIC). Clinical assessment of TF expression has remained a major challenge. No point-of-care assays are currently available to measure the level of TF activity in whole blood. The current study examined the suitability of the TiFaCT assay as a point-of-care assay to detect TF expression. METHODS:30 healthy male volunteers received 2 ng/kg of LPS. Tissue factor-dependent coagulation was quantified with a novel assay called tissue factor clotting time (TiFaCT), and by measurement of activation markers of downstream coagulation. RESULTS:Ex vivo addition of anti-TF antibodies to blood slightly increased clotting times at 0-24 h (p<0.01) indicating that some tissue factor activity was present in whole blood at any time. LPS bolus infusion decreased TiFaCT clotting time by -23% compared to baseline (p<0.01), when in vivo clotting increased, as demonstrated by a 10-fold increase in prothrombin fragment levels (F1+2). Ex vivo incubation with LPS considerably shortened TiFaCT (from 1000s to 400s as compared to control incubation; p<0.01). This effect was blunted at 2-4 h after LPS infusion (i.e. the time of monocytopenia), but twofold enhanced 24 h after LPS challenge (p<0.01). CONCLUSIONS:In summary, the TiFaCT assay was validated in our in vivo model of LPS-induced coagulation. It detected minute quantities of circulating TF even at baseline. TiFaCT is shortened at times of in vivo thrombin generation.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Marsik C,Quehenberger P,Mackman N,Osterud B,Luther T,Jilma Bdoi
10.1016/j.thromres.2003.09.017subject
Has Abstractpub_date
2003-01-01 00:00:00pages
311-5issue
4-5eissn
0049-3848issn
1879-2472pii
S0049384803004985journal_volume
111pub_type
临床试验,杂志文章,随机对照试验abstract：:The association between blood coagulation and cancer growth and metastatic dissemination is not yet completely understood. In this study we demonstrate that thrombin is capable of enhancing tumor cell adhesive properties and thereby increases tumor cell metastatic potential. Following exposure to alpha-thrombin, Walke...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(92)90081-k
更新日期：1992-11-01 00:00:00
abstract：:G619, a 4-OH-isophthalic acid derivative, was studied for its capacity to inhibit platelet aggregation. G619 dose-dependently inhibited U46619, collagen, ADP, PAF, thrombin and epinephrine-induced platelet aggregation in vitro. The IC50 values for inhibition of U46619-induced human and rabbit platelet aggregation were...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(92)90283-g
更新日期：1992-05-15 00:00:00
abstract：INTRODUCTION:Pulmonary embolism (PE) has a high morbidity and mortality. Hence it is important to recognize factors associated with higher risk of adverse outcomes in hemodynamically stable patients. Heart-type fatty acid binding protein (H-FABP) is a novel marker evaluated in recent years for prognosis in acute PE. Ou...
journal_title：Thrombosis research
pub_type： 杂志文章,meta分析
doi：10.1016/j.thromres.2014.10.007
更新日期：2015-01-01 00:00:00
abstract：:VKORC1 and VKORC1L1 are enzymes that both catalyze the reduction of vitamin K2,3-epoxide via vitamin K quinone to vitamin K hydroquinone. VKORC1 is the key enzyme of the classical vitamin K cycle by which vitamin K-dependent (VKD) proteins are γ-carboxylated by the hepatic γ-glutamyl carboxylase (GGCX). In contrast, t...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2015.01.025
更新日期：2015-05-01 00:00:00
abstract：INTRODUCTION:Incidence and risk factors of venous thromboembolism (VTE) are well established in surgical and medical settings, but data in psychiatric units are lacking. The aim of this study was to estimate the incidence of VTE in hospitalized psychiatric patients, and to assess the risk factors for VTE in this specif...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2012.10.002
更新日期：2012-12-01 00:00:00
abstract：:Preanalytical and biological variability both have strong influences on test results of coagulation. Beyond age and gender, increasing emphasis is now being given to a variety of demographic and lifestyle variables, including ethnicity, smoking, diet, and exercise, which should be taken into account when interpreting ...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2008.04.010
更新日期：2008-01-01 00:00:00
abstract：INTRODUCTION:To evaluate the pathogenetic role of tissue factor (TF), tissue factor pathway inhibitor (TFPI), and neutrophil elastase in acute respiratory distress syndrome (ARDS), as well as to test the hypothesis that TFPI levels modified by neutrophil activation are not sufficient to prevent TF-dependent intravascul...
journal_title：Thrombosis research
pub_type： 临床试验,杂志文章
doi：10.1016/s0049-3848(03)00151-8
更新日期：2003-01-25 00:00:00
abstract：:The use of platinum based chemotherapy in ovarian malignancy and other cancer types is known to be associated with deep vein thrombosis. In a prospective study of 47 patients with ovarian cancer of International Federation of Gynecology and Obstetrics stage Ib-IV, serial rheological parameters were determined (plasma ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(98)00022-x
更新日期：1998-04-15 00:00:00
abstract：:A new method, based on the observation that leukocytes contain PGI2-synthetase activity, was used to measure the effect of pharmacological levels of sulphinpyrazone on the TXA2 and PGI2 production in whole blood. Four human volunteers took 400 mg sulphinpyrazone twice daily for 5 1/2 days. Blood was drawn before the s...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(82)90033-0
更新日期：1982-10-01 00:00:00
abstract：:Capsaicin was found to be a potent inhibitor of platelet aggregation and release reaction. It inhibited the aggregation of rat platelet induced by collagen and thrombin, but only slightly reduced those of AA and A23187. The IC50 on collagen-induced platelet aggregation was about 85 micrograms/ml. Less inhibition was o...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(84)90189-0
更新日期：1984-12-15 00:00:00
abstract：:To examine whether moderate hyperhomocyst(e)inemia is associated with coronary artery disease and the number of diseased coronary vessels in Koreans, we investigated 201 patients with angiographically documented coronary artery disease and 187 healthy subjects without evidence of stroke and coronary artery disease. Th...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(98)00197-2
更新日期：1999-04-01 00:00:00
abstract：:Hypercoagulable states can be detected by measuring activation peptides, enzyme-inhibitor complexes, and fibrin/fibrinogen degradation products, which are markers of hemostatic activation. A series of these prethrombotic markers has been evaluated in the elderly, pregnancy, diabetes and acute myocardial infarction pat...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(98)00165-0
更新日期：1999-01-15 00:00:00
abstract：INTRODUCTION:This study determines the impact of tissue factor (TF)-signaling on the extrinsic pathway of apoptosis in cancer cells and propose death associated protein kinase-1 (DAPK1) as a novel key regulator. MATERIALS AND METHODS:In MDA-MB-231 breast and PC3 prostate cancer cells, mRNA levels were analyzed by real...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2010.11.015
更新日期：2011-02-01 00:00:00
abstract：:Activated platelets may adhere to leukocytes and form circulating mixed aggregates. The latter are considered a reliable marker of a prothrombotic state and are associated with several cardiovascular conditions. The molecular mechanisms responsible of this cellular interaction include a central role of platelet P-sele...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2011.10.010
更新日期：2012-03-01 00:00:00
abstract：:Concentration-response relationships of human platelet aggregation rates were analyzed for a variety of agonists and inhibitors. Their approximation by the Hill equation showed that the values of the Hill coefficient (h) were agonist-dependent and increased as follows: hADP = hL-EPINEPHRINE = hPAF = hPGH2 = hU46619 le...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(92)90308-w
更新日期：1992-06-01 00:00:00
abstract：BACKGROUND:The usefulness of coagulation tests performed before epidural analgesia for surgery or to alleviate labour pain is controversial. The aims of this study were: (1) to evaluate the prevalence of abnormal tests in a large cohort of healthy pregnant women and their association with epidural hematoma; (2) to asse...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2011.02.023
更新日期：2011-07-01 00:00:00
abstract：:Data on the effect of hormone replacement therapy on hemostasis are inconsistent, and there are few data in women with coronary artery disease. In a single-center, open, randomized study, 118 postmenopausal women with angiographically verified coronary artery disease were randomized to hormone replacement therapy, giv...
journal_title：Thrombosis research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0049-3848(99)00233-9
更新日期：2000-04-01 00:00:00
abstract：INTRODUCTION:Inter-individual variability in clopidogrel responsiveness is dependent on genetic polymorphisms. We aimed to investigate the impact of 3 genetic polymorphisms involved in clopidogrel metabolism on a strategy of dose-adjustment according to platelet reactivity (PR) monitoring. MATERIAL AND METHODS:[correc...
journal_title：Thrombosis research
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1016/j.thromres.2011.12.038
更新日期：2012-07-01 00:00:00
abstract：INTRODUCTION:Dermatan sulfate, a sulfated glycosaminoglycan, acts as an anticoagulant by accelerating the inhibition of thrombin by heparin cofactor II. MATERIALS AND METHODS:A low molecular mass dermatan sulfate was obtained by peroxy-radical depolymerization from a parent dermatan sulfate. Chemical characterization ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2007.09.001
更新日期：2008-01-01 00:00:00
abstract：:The enzymatic and fibrinolytic properties of glycosylated and nonglycosylated recombinant human pro-urokinase (pro-UK) produced in yeast Pichia pastoris were characterized and compared with those of Escherichia coli and mammalian cell-derived pro-UK. Among the five different forms of pro-UK, the yeast glycosylated pro...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(00)00349-2
更新日期：2000-12-01 00:00:00
abstract：:Diabetes mellitus (DM) is associated with platelet and leukocyte dysfunction. Previous observations with regard to insulin effects on platelet and leukocyte function are less than consistent. We thus investigated the effects of insulin on platelets and leukocytes, as well as on platelet-leukocyte interactions in whole...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(02)00306-7
更新日期：2002-09-01 00:00:00
abstract：INTRODUCTION:Inflammation and coagulation play important roles in the pathogenesis of sepsis. Anticoagulants with anti-inflammatory action draw attention as therapeutic agent in sepsis. OBJECTIVE:Whether cilostazol (6-[4-(1-cyclohexyl-1H-tetrazol-5-yl) butoxy]-3,4-dihydro-2-(1H)-quinolinone), anticoagulant, protects m...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2015.06.017
更新日期：2015-08-01 00:00:00
abstract：:Several clinical trials have demonstrated the benefits of thienopyridine monotherapy in ischaemic stroke patients. Non-human primate models of ischaemic stroke have been used for various antithrombotic agents; however, to the best of our knowledge, there is no data on the effects of P2Y12 antagonists in models, such a...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2015.09.013
更新日期：2015-12-01 00:00:00
abstract：BACKGROUND/AIM:It is believed that the amniotic fluid-derived TF, in the case of amniotic fluid embolism (AFE), contributes to acute disseminated intravascular coagulation (DIC) and obstetric shock in the mother. However, the role of amniotic fluid-derived contact phase coagulation factors that irrupt into the bloodstr...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2014.10.014
更新日期：2015-04-01 00:00:00
abstract：:Postoperative chylothorax is a frequently encountered pathology occurring in up to 4% of patients undergoing surgery for repair of congenital heart disease. Symptomatic thrombosis is associated with chylothorax and may contribute to its severity and duration. Furthermore, vessel thrombosis resulting in persistent vess...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2013.06.014
更新日期：2013-08-01 00:00:00
abstract：INTRODUCTION:Increased levels of circulating tissue factor (TF)-positive microvesicles (MVs) may increase the risk of thrombosis. Indeed, TF-positive MVs are detected in plasma of patients with various types of diseases. In this study, we measured levels of MV TF activity in non-cancer severely ill patients and cancer ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2018.03.001
更新日期：2018-05-01 00:00:00
abstract：INTRODUCTION:Venous thromboembolism (VTE) is a frequent clinical event in patients with gynecologic cancer. However, studies that exclusively address the incidence of VTE according the type of gynecologic cancer are poorly reported. OBJECTIVE:To analyze the incidence of VTE and the associated factors in women with dif...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2019.11.009
更新日期：2020-01-01 00:00:00
abstract：:Human platelets contain a single membrane glycoprotein which is susceptible to thrombin proteolysis, glycoprotein V. We have purified 1 mg of glycoprotein V from 10(13) platelets using a combination of gel filtration, hydroxylapatite and ion-exchange chromatographies. Glycoprotein V has a blocked amino-terminus. Follo...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(89)90113-8
更新日期：1989-01-01 00:00:00
abstract：:A 15 year-aged Japanese girl with a life long mild purpura was found as a variant type of thrombasthenia. Basic tests revealed prolonged bleeding time, border-line clot retraction, no coagulation defect, no giant platelets and mild thrombocytopenia (70,000-110,000/microliters). Neither ADP, epinephrine nor collagen ag...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(87)90146-0
更新日期：1987-08-01 00:00:00
abstract：:In this paper, we demonstrated that the procoagulant action of Lonomia achelous (Cramer) is due in part to a component that activates prothrombin. The activation by crude venom and Fractions obtained by gel filtration on Sephadex G-75 is not dependent of phospholipid, Ca++ or Factor V. The activation of prothrombin by...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(92)90187-f
更新日期：1992-05-01 00:00:00
