Abstract：
:A new method, based on the observation that leukocytes contain PGI2-synthetase activity, was used to measure the effect of pharmacological levels of sulphinpyrazone on the TXA2 and PGI2 production in whole blood. Four human volunteers took 400 mg sulphinpyrazone twice daily for 5 1/2 days. Blood was drawn before the study started, 3-4 h after the initial dosage, 12 h after the 10th dosage on the 5th day, and 3-4 h after the 11th (final) dosage on the 6th day. Collagen was added to the citrated blood samples to stimulate prostaglandin production. Aliquots were removed at regular intervals and TXB2 and 6-keto-PGF1 alpha measured by radioimmunoassay. The production of PGI2 was significantly inhibited in all the samples collected after sulphinpyrazone intake (p less than 0.01). The production of TXA2 was significantly inhibited only in the samples collected 3-4 h after sulphinpyrazone intake on the 6th day (p less than 0.01). These results confirmed the cyclooxygenase inhibitory action of sulphinpyrazone and also showed that in the whole blood system, PGI2 production was more effectively inhibited than TXA2 production.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Badenhorst PN,Deckmyn H,Vermylen Jdoi
10.1016/0049-3848(82)90033-0subject
Has Abstractpub_date
1982-10-01 00:00:00pages
59-66issue
1eissn
0049-3848issn
1879-2472pii
0049-3848(82)90033-0journal_volume
28pub_type
杂志文章abstract：BACKGROUND:Cerebral vein thrombosis (CVT) is a rare, life-threatening disease affecting one adult per 100,000 per year. Genetic risk factors are deficiencies of the natural anticoagulant proteins antithrombin, protein C, protein S or single nucleotide polymorphisms such as factor V Leiden and prothrombin 20210A. In 20%...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2018.06.011
更新日期：2018-09-01 00:00:00
abstract：:We have studied the synthesis and subcellular distribution of the glycosylated membrane protein tissue factor (TF) in human blood monocytes and the inducible monocyte like cell line, HL-60. Following induction with endotoxin or the phorbol ester, TPA, tissue factor specific activity was measured in intact cells, sonic...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(87)90405-1
更新日期：1987-11-15 00:00:00
abstract：:Hemophilia patients with inhibitors against FVIII/FIX present with a major challenge in the clinical practice. In the 1970s such patients were treated in association with emergency situations and essential surgery using huge amounts of FVIII/FIX concentrates often preceded by extracorporal adsorption of antibodies aga...
journal_title：Thrombosis research
pub_type： 历史文章,杂志文章,评审
doi：10.1016/j.thromres.2010.01.021
更新日期：2010-04-01 00:00:00
abstract：BACKGROUND:Deep vein thrombosis (DVT) is a common complication of acute stroke, but the new incidence in the era of improved specialist input in stroke care is yet unknown. The models for VTE diagnosis is well established, but prediction models to target at-risk patients for pharmacological prophylaxis is lacking and r...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2016.04.002
更新日期：2016-06-01 00:00:00
abstract：INTRODUCTION:Unfractionated heparin (UFH) does not cross the placenta and has demonstrated utility in the prevention and treatment of thrombosis during pregnancy. Limited information is available to guide initiation and monitoring of therapeutic UFH targeting an anti-Xa concentration of 0.3-0.7 u/ml during pregnancy. T...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2009.07.014
更新日期：2010-05-01 00:00:00
abstract：INTRODUCTION:Several hemostatic system components, including factor X (FX), contribute to cancer progression. The Protein Z (PZ)/protein Z-dependent protease inhibitor (ZPI) complex directly inhibits factor Xa proteolytic activity. The aim of this study was to determine the antigenic distribution of ZPI and PZ, in rela...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2011.10.027
更新日期：2012-04-01 00:00:00
abstract：:The influence of transmembrane Na+ and Ca2+ gradients on cytosolic pH (pHi) and free Ca2+ concentration ([Ca2+]i) have been examined in unstimulated human platelets with the aid of BCECF and Fura-2 fluorescent dyes. The removal of external Na+ (Na+o) acidified the cytosol in a pHo-dependent manner which was insensitiv...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(92)90233-z
更新日期：1992-01-15 00:00:00
abstract：:Experimental and clinical studies support the pivotal role played by reactive oxidant species in the mechanism of platelet activation. This effect is achieved via multiple pathways, including enhanced formation of isoprostanes. Platelet NADPH oxidase seems to be the main source of platelet reactive oxidant species and...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2011.12.002
更新日期：2012-03-01 00:00:00
abstract：INTRODUCTION:Vitamin K antagonists are often used for anticoagulant treatment in hip fracture patients. The optimal handling with such anticoagulants is unclear. We aimed to determine when anticoagulation reversal occurred after vitamin K administration and how often prothrombin complex concentrates (PCCs) were adminis...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2013.10.031
更新日期：2014-01-01 00:00:00
abstract：:The three main components involved in thrombosis and haemostasis are thrombin, platelets, and plasmin. Almost all inhibitors of thrombosis are focused either on the inhibition of thrombin or on the inhibition of platelets. We designed a construct using the fibrinolytic activity of staphylokinase, fused via a cleavable...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(97)00277-6
更新日期：1997-12-01 00:00:00
abstract：:The effect of several vitamin K (Vit K) analogues on the aggregation of human platelets was examined. The analogues were potent inhibitors of aggregation induced by ADP, thrombin, collagen and arachidonate but were less active against aggregation induced by the calcium ionophore A23187. Vit K3 also prevented platelet ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(85)90037-4
更新日期：1985-01-01 00:00:00
abstract：:Chloroquine inhibited aggregation of rat blood platelets in a dose-dependent way. The inhibitory effect decreased, depending on the aggregation stimulus used, in the rank order of potency: ADP > thrombin > A23187. Effect of 1 millimolar chloroquine on A23187-stimulated aggregation was significantly decreased or comple...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(94)90270-4
更新日期：1994-06-01 00:00:00
abstract：BACKGROUND:Upon platelet stimulation with agonists, reactive oxygen species (ROS) generation enhances platelet activation and granule release. Whether ROS generation during platelet storage could be directly correlated with the expression of proinflammatory molecules and granule release has been investigated in this st...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2017.06.016
更新日期：2017-08-01 00:00:00
abstract：:A platelet storage pool deficiency (SPD) is present in platelets from cattle with the Chediak-Higashi syndrome (CHS). The most plausible hypothesis for the SPD is that dense granule precursors are simply not formed in CHS megakaryocytes. There is, however, evidence that some recently acquired 5-hydroxytryptamine (5HT)...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(90)90096-u
更新日期：1990-05-01 00:00:00
abstract：:Clinical and epidemiological trials often involve central laboratory analyses of coagulation tests, including fibrinogen, which requires freezing of the plasma samples. Although rapid freezing by immersion of sample tubes in liquid nitrogen and storage at -70 degrees C is recommended, plasma samples are often transfer...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2008.11.010
更新日期：2009-05-01 00:00:00
abstract：:Recombinant factor IX (rFIX) is increasingly used to treat patients with hemophilia B. CHO (Chinese Hamster Ovary) cells are commonly used for the production of rFIX but they have a limited capacity for introducing post-translational modifications (PTM) leading to incomplete γ-carboxylation, low phosphorylation and su...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2012.08.313
更新日期：2012-11-01 00:00:00
abstract：:Optimal conditions for a method to simultaneously measure aggregation in 96 samples using a microplate reader were developed. The temperature of the assay was set at 25 degrees C, the optimal platelet concentration range was determined to be from 1-3 x 10(8) per mL, the assay volume was determined to be best at 100 mi...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(95)93881-y
更新日期：1995-03-01 00:00:00
abstract：IMPORTANCE:Unprovoked venous thromboembolism (VTE) can be the first manifestation of cancer. It is unclear if extensive screening for occult cancer including a comprehensive computed tomography (CT) scan of the abdomen/pelvis is cost-effective in this patient population. OBJECTIVE:To assess the health care related cos...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2017.01.007
更新日期：2017-03-01 00:00:00
abstract：INTRODUCTION:Changes in platelet count and function contribute to thrombo-hemorrhagic episodes in chronic myeloproliferative syndromes (MPS). We used flow cytometry to study platelet-leukocyte conjugates and markers of platelet activation in patients with MPS. METHODS:Whole blood from patients with chronic myelogenous...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(02)00354-7
更新日期：2002-11-01 00:00:00
abstract：:Venous thromboembolism remains an important cause of maternal mortality. For women at risk during pregnancy, the recommended venous thromboembolismprophylaxis is unfractionated heparin. Low molecular weight heparins, such as dalteparin, also may be suitable, but randomised trials have not been performed. Pregnant wome...
journal_title：Thrombosis research
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：10.1016/s0049-3848(99)00110-3
更新日期：1999-11-15 00:00:00
abstract：INTRODUCTION:Venous thromboembolism (VTE) occurs frequently in at-risk hospitalized patients, and prophylaxis of VTE is significantly underused. We sought to preliminarily assess the validity of Caprini risk assessment model, a famous individual VTE risk assessment model, in Chinese hospitalized patients with VTE. MAT...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2012.08.001
更新日期：2012-11-01 00:00:00
abstract：:An in vitro test system was developed to examine the effects of red blood cells (RBC) on shear-induced platelet adhesion (SIPAD) and platelet aggregation (SIPAG). Suspensions of human platelets labeled with Mepacrine and suspended in citrated plasma were exposed to single, continuous or repetitive (120-300x) one secon...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(98)00160-1
更新日期：1998-12-15 00:00:00
abstract：BACKGROUND:Acquired von Willebrand Syndrome is a rare bleeding disorder, which arises in individuals with no personal or family history of bleeding, associated with lymphoproliferative and myeloproliferative disorders or other diseases. AIM:To develop a two-step approach assay to detect autoantibodies against VWF and ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2014.09.020
更新日期：2014-12-01 00:00:00
abstract：INTRODUCTION:In the prospective, randomised, double-blind, placebo-controlled Regenerate Vital Myocardium by Vigorous Activation of Bone Marrow Stem Cells (REVIVAL)-2 trial patients with acute myocardial infarction (AMI) and successful mechanical reperfusion received granulocyte-colony stimulating factor (G-CSF, 10 μg/...
journal_title：Thrombosis research
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.thromres.2010.11.004
更新日期：2011-02-01 00:00:00
abstract：:Prothrombin fragments (F1+2), thrombin-antithrombin complexes (TAT) and D-dimers, markers of hemostatic system activation, were measured in 59 consecutive patients with deep vein thrombosis (DVT). Patients were randomly treated either with subcutaneous unfractionated heparin (UH) administered in two to three subcutane...
journal_title：Thrombosis research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0049-3848(02)00023-3
更新日期：2002-02-01 00:00:00
abstract：:A significantly higher platelet malondialdehyde-like material (MDA--LM) content after physical and N-ethylmaleimide (NEM) stimulation is found in chronic ischemic heart disease (CHD) patients when compared to the control group. In subjects under aspirin treatment "in vivo" (1 gr/day) no difference is found between CHD...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(82)90305-x
更新日期：1982-09-01 00:00:00
abstract：BACKGROUND:There is a need to identify and develop novel thrombolytic agents that can directly digest fibrin clots from various biological resources. OBJECTIVE:To clone, express, purify, and characterize a recombinant protease named rvFMP capable of cleaving fibrinogen, fibrin polymer, and cross-linked fibrin in human...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2020.04.040
更新日期：2020-07-01 00:00:00
abstract：:Arterial thrombosis and venous thromboembolism (VTE) are traditionally considered two different entities. However, patients with unprovoked VTE are at higher risk of developing subclinical and overt atherosclerosis compared to healthy controls challenging these distinctions. Obesity may explain the association between...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2020.04.035
更新日期：2020-07-01 00:00:00
abstract：INTRODUCTION:Proteasome inhibitor bortezomib (PS-341) has been the first proteasome inhibitor that has entered clinical trials with its antiproliferative and proapoptotic effects in patients with multiple myeloma. Recent studies indicate that proteasome inhibitors can be useful in prevention of experimental arterial th...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2007.06.013
更新日期：2008-01-01 00:00:00
abstract：:Thrombin-activatable fibrinolysis inhibitor (TAFI), also known as procarboxypeptidase U or plasma procarboxypeptidase B, is a relatively recently described plasma glycoprotein synthesised in the liver. It can be activated into active enzyme TAFIa (carboxypeptidase U or plasma carboxypeptidase B) by a complex of thromb...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(02)00072-5
更新日期：2002-04-01 00:00:00
