当前位置： SCI文献检索 > THROMBOSIS RESEARCH期刊下所有文献 > Markers of platelet activation and platelet-leukocyte interaction in patients with myeloproliferative syndromes.

Markers of platelet activation and platelet-leukocyte interaction in patients with myeloproliferative syndromes.

Abstract：

INTRODUCTION:Changes in platelet count and function contribute to thrombo-hemorrhagic episodes in chronic myeloproliferative syndromes (MPS). We used flow cytometry to study platelet-leukocyte conjugates and markers of platelet activation in patients with MPS. METHODS:Whole blood from patients with chronic myelogenous leukemia (CML), polycythemia vera (PV), chronic myelofibrosis (MF), and essential thrombocythemia (ET) and from healthy volunteers was prepared for flow cytometry. Platelet microparticles and platelet microaggregates were identified with anti-CD42b and forward scatter, activated platelets with anti-CD62p. Anti-CD42b, anti-CD14, and anti-CD45 were used to study platelet-leukocyte conjugates. RESULTS:The percentage of CD62p-positive platelets was elevated in all myeloproliferate syndrome subtypes. The median percentage of platelet microparticles was 5.2% in controls and significantly higher in PV (12.0%), MF (11.0%), and ET (11.0%, all p<0.05). There was an increased percentage of platelet-neutrophil conjugates in patients with PV (8.3%) and ET (10.4%) compared to normal controls (6.8%, all p<0.05). Platelet-monocyte conjugates were 8.0% in controls and elevated in PV (15.4%) and ET (15.0%, all p<0.05). Patients with a history of venous or arterial thrombotic events had slightly less platelet-leukocyte conjugates and slightly more microparticles than patients without thrombosis; however, this difference was not statistically significant. CONCLUSIONS:These findings suggest that platelet-leukocyte conjugate formation occurs in myeloproliferative syndromes and indicates platelet activation. Also, platelet microparticles are elevated and might provide a catalytic surface for thrombin generation. This could explain the clinical observation that patients with myeloproliferative syndromes have an increased risk to experience arterial or venous thrombotic events.

journal_name

Thromb Res

journal_title

Thrombosis research

authors

Villmow T,Kemkes-Matthes B,Matzdorff AC

doi

10.1016/s0049-3848(02)00354-7

subject

Has Abstract

pub_date

2002-11-01 00:00:00

pages

139-45

issue

2-3

eissn

0049-3848

issn

1879-2472

pii

S0049384802003547

journal_volume

108

pub_type

杂志文章

相关文献

THROMBOSIS RESEARCH文献大全
  • Efficacy and safety of prolonged thromboprophylaxis with a low molecular weight heparin (dalteparin) after total hip arthroplasty--the Danish Prolonged Prophylaxis (DaPP) Study.

    abstract：:The aim of this study was to compare the efficacy and safety of prolonged (35 days) thromboprophylaxis with a standard length (7 days) regimen of a low molecular weight heparin in patients undergoing total hip arthroplasty. The study was multicentre, randomised, double-blind, and prospective with two groups. Following...

    journal_title：Thrombosis research

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1016/s0049-3848(98)00018-8

    authors： Lassen MR,Borris LC,Anderson BS,Jensen HP,Skejø Bro HP,Andersen G,Petersen AO,Siem P,Hørlyck E,Jensen BV,Thomsen PB,Hansen BR,Erin-Madsen J,Møller JC,Rotwitt L,Christensen F,Nielsen JB,Jørgensen PS,Paaske B,Tørholm

    更新日期：1998-03-15 00:00:00

  • Effects of verapamil on platelet aggregation, ATP release and thromboxane generation.

    abstract：:We examined the effects of verapamil on platelet function. Verapamil (0.5 micrograms/ml) in this study inhibited platelet aggregation induced by threshold amounts of ADP, AA, and epinephrine. With higher concentrations of aggregating stimuli, verapamil caused a dose-dependent inhibition of aggregation. Verapamil reduc...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(83)90181-0

    authors： Mehta J,Mehta P,Ostrowski N,Crews F

    更新日期：1983-06-01 00:00:00

  • A two-step approach (Enzyme-linked immunosorbent assay and confirmation assay) to detect antibodies against von Willebrand factor in patients with Acquired von Willebrand Syndrome.

    abstract：BACKGROUND:Acquired von Willebrand Syndrome is a rare bleeding disorder, which arises in individuals with no personal or family history of bleeding, associated with lymphoproliferative and myeloproliferative disorders or other diseases. AIM:To develop a two-step approach assay to detect autoantibodies against VWF and ...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2014.09.020

    authors： Franchi F,Biguzzi E,Stufano F,Siboni SM,Baronciani L,Peyvandi F

    更新日期：2014-12-01 00:00:00

  • Differential effects of low molecular weight heparin and unfractionated heparin on circulating levels of antithrombin and tissue factor pathway inhibitor (TFPI): a possible mechanism for difference in therapeutic efficacy.

    abstract：:There is growing evidence on the superior efficacy and safety of low molecular weight heparins (LMWHs) over unfractionated heparin (UFH) for the treatment of both venous and arterial thromboembolism. Heparin exerts its function by potentiating antithrombin and by mobilizing tissue factor pathway inhibitor (TFPI) into ...

    journal_title：Thrombosis research

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1016/s0049-3848(98)00079-6

    authors： Hansen JB,Sandset PM

    更新日期：1998-08-15 00:00:00

  • Thromboembolic complications in the nephrotic syndrome: pathophysiology and clinical management.

    abstract：:Patients with the nephrotic syndrome are at increased risk of developing venous and arterial thromboembolism, the most common of which is renal vein thrombosis. There are several unanswered or controversial issues relating to the nephrotic syndrome and thromboembolism, which include the mechanism of thromboembolism, a...

    journal_title：Thrombosis research

    pub_type： 杂志文章,评审

    doi：10.1016/j.thromres.2005.03.030

    authors： Singhal R,Brimble KS

    更新日期：2006-01-01 00:00:00

  • Antithrombotic activity of a symmetrical triglyceride with eicosapentaenoic acid and gamma-linolenic acid in guinea pig mesenteric microvasculature.

    abstract：:The antithrombotic effect of a synthetic symmetrical triglyceride having eicosapentaenoic acid (EPA) at positions 1 and 3, and gamma-linolenic acid (GLA) at position 2 was investigated. Administration of the triglyceride significantly increased thrombus formation time and thrombotic occlusion time induced by light irr...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(95)99610-k

    authors： Akahane N,Ohba S,Suzuki J,Wakabayashi T,Nakahara T,Yanagi K,Ohshima N

    更新日期：1995-06-01 00:00:00

  • Effects of fibrinogen and platelet supplementation on clot formation and platelet aggregation in blood samples from cardiac surgery patients.

    abstract：INTRODUCTION:Bleeding after cardiac surgery may be caused by surgical factors, impaired haemostasis, or a combination of both. Transfusion of blood products is used to improve haemostasis, but little is known about what combination is optimal. We hypothesized that addition of both fibrinogen and platelets to blood samp...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2014.05.023

    authors： Shams Hakimi C,Fagerberg Blixter I,Hansson EC,Hesse C,Wallén H,Jeppsson A

    更新日期：2014-10-01 00:00:00

  • Kinetic study on the initial stage of the fibrinogen-fibrin conversion by thrombin. (IV). Effects of heparin and its analogues.

    abstract：:From kinetic analysis on the initial stage of the fibrinogen-fibrin conversion catalyzed by thrombin, inhibition constants, Kip, of heparin and heparin analogues were obtained by the turbidimetrical method. The inhibitory effect per unit amount in weight of heparin and heparin analogues increased generally with decrea...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(85)90235-x

    authors： Sato H,Nakajima A,Shimohira T

    更新日期：1985-09-01 00:00:00

  • Evaluation of two functional assays for protein C inhibitor/plasminogen activator inhibitor-3 activity.

    abstract：:Two functional assays for protein C inhibitor (PCI) were evaluated in parallel for the same plasma samples. One assay is specific for active PCI and measures its ability to form complexes with activated protein C (APC) in the presence of heparin (España et al, Thromb Res 64, 309, 1991) (Method A). After incubation of ...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(93)90079-4

    authors： España F,Hendl S,Gilabert J,Estellés A,Aznar J

    更新日期：1993-06-01 00:00:00

  • Genetic score based on high-risk genetic polymorphisms and early onset of ischemic heart disease in an Italian cohort of ischemic patients.

    abstract：:Several single-nucleotide polymorphisms (SNPs) have been recognized as associated with ischemic heart disease (IHD) although the optimal set of risk genotypes has not be identified. This study aimed to examine whether identified high-risk SNPs are associated with early onset of IHD. In the GENOCOR study, 44 high-risk ...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2014.03.006

    authors： Vecoli C,Adlerstein D,Shehi E,Bigazzi F,Sampietro T,Foffa I,Carpeggiani C,L'abbate A,Andreassi MG

    更新日期：2014-05-01 00:00:00

  • A case-control study provides evidence of association for a common SNP rs974819 in PDGFD to coronary heart disease and suggests a sex-dependent effect.

    abstract：INTRODUCTION:Peden et al. have revealed a significant association between four new risk loci and coronary heart disease (CHD) in Europeans and South Asians. The goal of this study is to evaluate the contribution of these genetic loci to CHD risk in Han Chinese. METHODS:We recruited 161 CHD patients and 112 controls pr...

    journal_title：Thrombosis research

    pub_type： 杂志文章,meta分析

    doi：10.1016/j.thromres.2012.05.023

    authors： Zhou J,Huang Y,Huang RS,Wang F,Xu L,Le Y,Yang X,Xu W,Huang X,Lian J,Duan S

    更新日期：2012-10-01 00:00:00

  • Murine models of vascular endothelial injury: Techniques and pathophysiology.

    abstract：:Vascular endothelial injury (VEI) triggers pathological processes in various cardiovascular diseases, such as coronary heart disease and hypertension. To further elucidate the in vivo pathological mechanisms of VEI, many animal models have been established. For the easiness of genetic manipulation and feeding, murine ...

    journal_title：Thrombosis research

    pub_type： 杂志文章,评审

    doi：10.1016/j.thromres.2018.07.014

    authors： Wu Y,Su SA,Xie Y,Shen J,Zhu W,Xiang M

    更新日期：2018-09-01 00:00:00

  • EUHASS: The European Haemophilia Safety Surveillance system.

    abstract：:Pharmacovigilance is an essential element of any drug treatment and considering the history of adverse events due to products used to treat inherited bleeding disorders, it should be an integral component of modern haemophilia treatment. Because inherited bleeding disorders and adverse events are rare, a multicentre, ...

    journal_title：Thrombosis research

    pub_type： 杂志文章,多中心研究

    doi：10.1016/S0049-3848(10)70150-X

    authors： Makris M,Calizzani G,Fischer K,Gilman EA,Hay CR,Lassila R,Lambert T,Ludlam CA,Mannucci PM

    更新日期：2011-01-01 00:00:00

  • Detection of hypofibrinolysis in stable coronary artery disease using the overall haemostatic potential assay.

    abstract：INTRODUCTION:Patients with stable coronary artery disease (CAD) are at risk of arterial thrombosis causing myocardial infarction. Detection of global haemostatic markers of hypercoagulability and hypofibrinolysis may be important for risk stratification and individualised treatment. We examined overall haemostatic pote...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2013.03.015

    authors： Reddel CJ,Curnow JL,Voitl J,Rosenov A,Pennings GJ,Morel-Kopp MC,Brieger DB

    更新日期：2013-05-01 00:00:00

  • Safety of continuing warfarin therapy during cataract surgery: a systematic review and meta-analysis.

    abstract：BACKGROUND:In patients who are receiving warfarin therapy and require cataract surgery, it may be possible to continue warfarin in the perioperative period but the safety of this management strategy has not been systematically evaluated. METHODS:We performed a systematic review of the literature to assess the safety (...

    journal_title：Thrombosis research

    pub_type： 杂志文章,meta分析,评审

    doi：10.1016/j.thromres.2009.01.007

    authors： Jamula E,Anderson J,Douketis JD

    更新日期：2009-07-01 00:00:00

  • Oxidized lipoprotein(a) enhanced the expression of P-selectin in cultured human umbilical vein endothelial cells.

    abstract：:Lipoprotein(a) [Lp(a)] has been demonstrated to be an independent risk factor for coronary heart disease. However, the precise mechanism by which it contributes to the development of atherosclerosis remains unclear. P-selectin is one of the major adhesion molecules to mediate the interaction between monocytes, platele...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/s0049-3848(00)00363-7

    authors： Zhao SP,Xu DY

    更新日期：2000-12-15 00:00:00

  • B cell biology, apoptosis, and autoantibodies to phospholipids.

    abstract：:Systemic autoimmune diseases are characterised by the development of a relatively narrow spectrum of autoantibodies. These are of considerable diagnostic value. In addition, in some diseases, including anti-phospholipid syndrome, these autoantibodies can be directly pathogenic. Understanding how these autoantibodies a...

    journal_title：Thrombosis research

    pub_type： 杂志文章,评审

    doi：10.1016/j.thromres.2004.06.037

    authors： Cook MC

    更新日期：2004-01-01 00:00:00

  • Endoglycosidase H digestion of Yukb (Pena) alloantigen.

    abstract：:We characterized a platelet specific alloantigen (Yukb). In immunoblotting, anti-Yukb antibody was found to react with both 110 kDa and 96 kDa bands under nonreducing condition. Immunoblotting followed by separation by two-dimensional electrophoresis (isoelectric focusing/SDS-PAGE) showed that the 96 kDa band had a pI...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(92)90136-x

    authors： Sugano W,Ryo R,Yamaguchi N,Shibata Y

    更新日期：1992-07-15 00:00:00

  • Calibrated automated thrombography demonstrates hypercoagulability in patients with idiopathic pulmonary arterial hypertension.

    abstract：INTRODUCTION:Pathogenesis of idiopathic pulmonary arterial hypertension (iPAH) includes endothelial dysfunction and in situ thrombosis. A hypercoagulable state has also been postulated but never demonstrated. Our objective was to determine whether patients with iPAH had a hypercoagulable state using calibrated automate...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2010.08.020

    authors： Tournier A,Wahl D,Chaouat A,Max JP,Regnault V,Lecompte T,Chabot F

    更新日期：2010-12-01 00:00:00

  • Immunopurification of an S-antigen-like protein from human platelets.

    abstract：:S-antigen (also named arrestin or 48K protein) is a protein abundant in photoreceptor cells of vertebrates and invertebrates. The presently known function of this protein in retina is to arrest the enzymatic cascade of phototransduction in retinal rods, through its binding to photoactivated and phosphorylated rhodopsi...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：

    authors： Mirshahi M,Razaghi A,Mirshahi SS,Van Tuyen V,Faure JP

    更新日期：1991-12-01 00:00:00

  • Lonofibrase, a novel alpha-fibrinogenase from Lonomia obliqua caterpillars.

    abstract：:Envenomation caused by Lonomia obliqua caterpillars is an increasing problem in Southern Brazil. The clinical profile is characterized by a profound hemorrhagic disorder. In the present study, we describe the characterization of a fibrin(ogen)olytic factor (lonofibrase) isolated from a venomous secretion of the caterp...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2004.01.012

    authors： Pinto AF,Dobrovolski R,Veiga AB,Guimarães JA

    更新日期：2004-01-01 00:00:00

  • Helenalin and 11 alpha,13-dihydrohelenalin, two constituents from Arnica montana L., inhibit human platelet function via thiol-dependent pathways.

    abstract：:This study investigates the effect on human platelet function of two sesquiterpene lactones from Arnica montana L., helenalin (H) and 11 alpha,13-dihydrohelenalin (DH). Both compounds inhibited collagen-induced platelet aggregation, thromboxane formation and 5-hydroxytryptamine secretion in a concentration-dependent m...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(90)90151-2

    authors： Schröder H,Lösche W,Strobach H,Leven W,Willuhn G,Till U,Schrör K

    更新日期：1990-03-15 00:00:00

  • Properties of the complex between alpha 2-macroglobulin and brinase, a proteinase from Aspergillus oryzae with thrombolytic effect.

    abstract：:The proteinase, brinase (Mr approximately 35000), from Aspergillus oryzae, which has been used in therapeutic attempts as a thrombolytic agent in arterial thrombosis, binds to purified human alpha 2-macroglobulin (alpha 2M) with a stoichiometry of 1.7-1.9 mol of enzyme/mol inhibitor. This binding leads to quantitative...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(88)90359-3

    authors： Larsson LJ,Frisch EP,Törneke K,Lindblom T,Björk I

    更新日期：1988-01-01 00:00:00

  • Long-term effects of ticlopidine on fibrinogen and haemorheology in patients with peripheral arterial disease.

    abstract：:The effects of ticlopidine treatment (250 mg b.i.d. for 21 months) on fibrinogen and other rheological variables, as compared to placebo, were studied in 44 patients with intermittent claudication due to peripheral arterial occlusive disease. Blood samples were collected every 3 months during this double-blind, random...

    journal_title：Thrombosis research

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1016/0049-3848(88)90134-x

    authors： Palareti G,Poggi M,Torricelli P,Balestra V,Coccheri S

    更新日期：1988-12-15 00:00:00

  • Clinical evaluation of a pasteurized factor XIII concentrate administration in Henoch-Schönlein purpura. Japanese Pediatric Group.

    abstract：:Arthral, abdominal and renal symptoms in Henoch-Schönlein purpura (HSP) were scored. Coagulation factor XIII (F XIII) activity was determined in fifty-six children with HSP and the correlation with the severity score of the clinical symptoms was investigated. As a result, it was found that the decrease in F XIII level...

    journal_title：Thrombosis research

    pub_type： 临床试验,杂志文章,多中心研究

    doi：10.1016/0049-3848(89)90284-3

    authors： Fukui H,Kamitsuji H,Nagao T,Yamada K,Akatsuka J,Inagaki M,Shike S,Kobayashi Y,Yoshioka K,Maki S

    更新日期：1989-12-15 00:00:00

  • C-terminal peptide alcohol, acid and amide analogs of desulfato hirudin54-65 as antithrombin agents.

    abstract：:Analogs of the antithrombin peptide hirudin54-65 with C-terminal modifications have been synthesized in order to examine the requirements for alpha-thrombin inhibition. The C-terminal residue, Gln65, could be replaced with L-amino acids or amino alcohols with neutral or charged hydrophilic side chains without greatly ...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(89)90090-x

    authors： Krstenansky JL,Payne MH,Owen TJ,Yates MT,Mao SJ

    更新日期：1989-05-15 00:00:00

  • ATP-secretion occurs as an initial response in collagen induced platelet activation.

    abstract：:The time course and degree of collagen induced platelet shape change, aggregation and ATP-secretion in citrated human platelet rich plasma was monitored with a lumi-aggregometer. Onset of ATP-secretion was measurable within seconds after collagen challenge and always distinctly before onset of shape change. In absence...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(86)90089-7

    authors： Malmgren R

    更新日期：1986-08-15 00:00:00

  • Antithrombogenic effects of calcium channel blockers: synergism with prostacyclin and thromboxane synthase inhibitors.

    abstract：:Four calcium channel blockers (nimodipine, nifedipine, verapamil and diltiazem) of three chemical classes were tested in vitro for inhibition of platelet aggregation using heparinized human platelet rich plasma. Both ADP- and thrombin-induced aggregation were inhibited as was the biosynthesis of thromboxane A2 in resp...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(84)90241-x

    authors： Onoda JM,Sloane BF,Honn KV

    更新日期：1984-06-01 00:00:00

  • Influence of endothelial nitric oxide synthase gene polymorphisms (-786T>C, 4a4b, 894G>T) in Korean patients with coronary artery disease.

    abstract：INTRODUCTION:Endothelium-derived nitric oxide (NO) is synthesized from l-arginine by endothelial nitric oxide synthase (eNOS) encoded by the eNOS3 gene on chromosome 7. The effects of the eNOS polymorphisms with the risk of coronary artery disease are conflicting. In this study, we investigated the association of the e...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2006.06.005

    authors： Kim IJ,Bae J,Lim SW,Cha DH,Cho HJ,Kim S,Yang DH,Hwang SG,Oh D,Kim NK

    更新日期：2007-01-01 00:00:00

  • Interleukin 1 enhances arterial thrombogenicity in vitro.

    abstract：:An in vitro model of platelet adhesion, the annular perfusion chamber, was utilized to test the effects of Interleukin 1 on vascular thrombogenicity for platelets. De-endothelialized, everted human umbilical arteries were placed in cell culture media with or without Interleukin 1. The arteries were later perfused with...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(89)90235-1

    authors： Schorer AE,White JG

    更新日期：1989-11-15 00:00:00