Abstract：
:There is growing evidence on the superior efficacy and safety of low molecular weight heparins (LMWHs) over unfractionated heparin (UFH) for the treatment of both venous and arterial thromboembolism. Heparin exerts its function by potentiating antithrombin and by mobilizing tissue factor pathway inhibitor (TFPI) into the circulation. The present study was conducted to compare the effect of subcutaneous LMWH and infusion of UFH on these two anticoagulants in plasma. Eighteen healthy male volunteers were randomly allocated to therapy with continuous intravenous (iv) UFH (n=6) (initial infusion rate 450 IU/kg/day) or low molecular weight heparin (LMWH) (enoxaparin, 1.5 mg/kg/day) subcutaneously (sc) once daily for 72 hours. Free TFPI antigen, measured by a solid-phase two-site enzyme immunoassay, and antithrombin and protein C activities, measured by chromogenic assays, were assessed in plasma samples before, during, and after anticoagulant treatment. Infusion of UFH, but not subcutaneous LMWH, was found to attenuate the antithrombotic defense by a selective decrease of both circulating antithrombin (-21+/-7%, p<0.0001) and of free and endothelial-bound TFPI. The changes in antithrombin and TFPI by LMWH and UFH were statistically different between groups (p<0.001). The differential effect of UFH and LMWH on antithrombin and TFPI may explain the superior efficacy of subcutaneous LMWH compared with conventional intravenous UFH for treatment of both arterial and venous thrombosis.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Hansen JB,Sandset PMdoi
10.1016/s0049-3848(98)00079-6subject
Has Abstractpub_date
1998-08-15 00:00:00pages
177-81issue
4eissn
0049-3848issn
1879-2472pii
S0049-3848(98)00079-6journal_volume
91pub_type
临床试验,杂志文章,随机对照试验abstract：:A direct assay for plasminogen activator (PA) was developed. It employed polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and beta-mercaptoethanol to monitor PA-mediated conversion of single chain, 125I-plasminogen to two chain plasmin. By incorporating Triton X-100, albumin and trasylol in...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(84)90008-2
更新日期：1984-05-01 00:00:00
abstract：INTRODUCTION:Inter-individual variability in clopidogrel responsiveness is dependent on genetic polymorphisms. We aimed to investigate the impact of 3 genetic polymorphisms involved in clopidogrel metabolism on a strategy of dose-adjustment according to platelet reactivity (PR) monitoring. MATERIAL AND METHODS:[correc...
journal_title：Thrombosis research
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1016/j.thromres.2011.12.038
更新日期：2012-07-01 00:00:00
abstract：:Murine monoclonal antibodies (mAb) were raised to a purified product of bovine PF-4, a 9,500 dalton protein with heparin neutralization activity comparable to that of human PF-4. Using a non-radioactive slide immunoenzymatic assay, four major classes of mAb could be identified when comparisons were made between purifi...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(86)90384-1
更新日期：1986-03-15 00:00:00
abstract：BACKGROUND:Anticoagulated patients are often seen unnecessarily in the emergency department (ED) for epistaxis, leading to increased healthcare costs. Patients are often unaware of preventative and management techniques for handling epistaxis in the home. METHODS:In 2016, the Michigan Anticoagulation Quality Improveme...
journal_title：Thrombosis research
pub_type： 信件
doi：10.1016/j.thromres.2021.01.007
更新日期：2021-01-16 00:00:00
abstract：:Both venous thromboembolism and intracranial metastases are common complications in the setting of primary brain tumors and metastatic malignancies. Anticoagulation is indicated in the presence of cancer-associated thrombosis in order to limit the risk of pulmonary embolism; however, there is reluctance to initiate an...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/S0049-3848(16)30100-1
更新日期：2016-04-01 00:00:00
abstract：INTRODUCTION:Bleeding after cardiac surgery may be caused by surgical factors, impaired haemostasis, or a combination of both. Transfusion of blood products is used to improve haemostasis, but little is known about what combination is optimal. We hypothesized that addition of both fibrinogen and platelets to blood samp...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2014.05.023
更新日期：2014-10-01 00:00:00
abstract：:The hemolytic uremic syndrome involves the presence of Shiga toxin producing strains of Escherichia coli and is associated with thrombocytopenia, platelet activation, and microthrombi formation. We have, therefore, investigated the ability of Shiga toxin isotypes 1 and 2 to cause or enhance platelet aggregation under ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(00)00191-2
更新日期：2000-06-01 00:00:00
abstract：:A series of 8 analogues of platelet-activating factor (PAF) in which both the acetyl group and the polar head are changed, were tested for biological activities on human and rabbit platelets in plasma. Two of these compounds, 1-O-hexadecyl-2-O-ethyl-rac-glycero-3-phosphoric acid-5'-trimethylammoniumpentyl ester and 1-...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(88)90244-7
更新日期：1988-02-15 00:00:00
abstract：:Protein C (PC) is the central protein in a major antithrombotic regulatory mechanism. Hereditary deficiencies of PC are associated with thrombosis. Therapeutic PC replacement may be an important treatment if pure functional human protein C is available in sufficient quantity. Human PC has been produced on a commercial...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(90)90258-e
更新日期：1990-02-01 00:00:00
abstract：:The study of the interaction between activated protein C (APC) and non-plasmatic inhibitors allowed us to demonstrate that aprotinin is a potent competitive inhibitor of APC with a Ki of 1.35 mumol/L. It was possible to adsorb immunopurified protein C (PC) activated by venom activator to insolubilized aprotinin and to...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(90)90268-h
更新日期：1990-07-01 00:00:00
abstract：BACKGROUND:Rate of thalidomide-related VTE and risk factors in Japanese myeloma patients are not clear and effects of thromboprophylaxis remain controvertial. PATIENTS AND METHODS:We retrospectively analyzed a cohort data of registered Japanese myeloma patients treated with thalidomide-based regimens between 2009 and ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2012.11.014
更新日期：2013-02-01 00:00:00
abstract：:The effects of locally deposited fibrin and of tranexamic acid-induced antifibrinolysis on forming granulation tissue were studied in the light of a recently developed method for treatment of postoperative fistulas by occlusion with a fibrin clot. Perforated teflon cylinders, either empty or fibrin-filled, were implan...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(84)90152-x
更新日期：1984-01-01 00:00:00
abstract：:Hemophilia A is an X-linked recessive bleeding disorder, widely prevalent throughout the world, for which, replacement therapy is the current treatment done by infusion of either recombinant FVIII or human plasma derived FVIII. The expression of FVIII is limited by many aspects, bioengineered FVIII with increased the ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2011.07.015
更新日期：2011-12-01 00:00:00
abstract：:Pergularain e I, a cysteine protease with thrombin-like activity, was purified by ion exchange chromatography from the latex of Pergularia extensa. Its homogeneity was characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), native PAGE and reverse-phase high-performance liquid chromatogr...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2009.10.002
更新日期：2010-03-01 00:00:00
abstract：:Many patients with diabetes mellitus show increased platelet aggregation and prostaglandin synthesis in response to physiological agents such as ADP and collagen when their platelets are tested in platelet-rich plasma or washed platelet suspensions. However, the relationship between increased platelet aggregation in v...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(87)90265-9
更新日期：1987-06-01 00:00:00
abstract：:Prothrombin time is a standard screening test to monitor patients treated with oral anticoagulants. We hypothesize that INR measurements will not vary a lot within a given day in a given patient. The present study was thus conducted to compare the change of the international normalized ratio (INR) of prothrombin time ...
journal_title：Thrombosis research
pub_type： 临床试验,杂志文章
doi：10.1016/s0049-3848(02)00339-0
更新日期：2002-09-01 00:00:00
abstract：:Defibrotide both "in vitro" and "ex vivo" decreases PMN adhesion to nylon columns. This activity is blunted by 8-phenyltheophylline, a known blocker of adenosine A1/A2 receptors, suggesting that Defibrotide acts on that kind of receptors. Indomethacin, a blocker of PGI2 synthesis, did not cancel DFT's activity, sugges...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(95)00178-t
更新日期：1995-11-15 00:00:00
abstract：:An experimental in vitro model of the hemodynamics that occur in atrial fibrillation (AFib) in the left atrial appendage (LAA) was developed to study changes in human endothelial cell thrombotic potential. We applied human-derived sinus rhythm and AFib hemodynamic shear stress patterns to primary human endothelial cel...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2016.04.022
更新日期：2016-07-01 00:00:00
abstract：:The activity of tissue plasminogen activator-plasminogen activator inhibitor-1 (t-PA-PAI-1) complex to convert plasminogen to plasmin was examined by using fibrin autography and casein zymography where plasminogen was added or removed for casein layer. Fibrin autography was more sensitive to the activity of t-PA even ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(91)90279-6
更新日期：1991-07-01 00:00:00
abstract：OBJECTIVES:Dabigatran etexilate is widely used for stroke prevention in the patients with atrial fibrillation. The anticoagulation activity of dabigatran is not necessary monitored in routine clinical practice. We aimed to study the effect of dabigatran on thrombin generation (TG) and coagulation assays in rabbit and h...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2018.03.012
更新日期：2018-05-01 00:00:00
abstract：INTRODUCTION:Whether clinical prediction rules for pulmonary embolism are accepted and used among general internal medicine residents remains uncertain. We therefore evaluated the frequency of use and acceptability of the Revised Geneva Score (RGS) and the Pulmonary Embolism Severity Index (PESI), and explored which fa...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2017.09.028
更新日期：2017-12-01 00:00:00
abstract：:Five criteria for poor response to a 20 min venous occlusion test were applied to 58 patients 3 months or more after acute deep vein thrombosis (DVT). The criteria were arbitrarily defined as the last 5 percentiles of response distributions in an age- and sex-matched healthy control group of 51 subjects. The criteria ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(91)90345-w
更新日期：1991-11-15 00:00:00
abstract：BACKGROUND:The Clauss fibrinogen method and thrombin clotting time (TCT) are still routinely used in patients with cirrhosis to define fibrinogen concentration and clotting potential. The thromboelastometric functional fibrinogen FIBTEM assay evaluates the strength of fibrin-based clots in whole blood, providing inform...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2015.04.003
更新日期：2015-06-01 00:00:00
abstract：:To estimate the effect of dilazep in preventing restenosis after a transluminal angioplasty, we have attempted an animal experiment in which the efficacy of drug was tested on an angioplastic injury superimposed on to atherosclerotic lesions. Using a modification of Block's method, atherosclerotic lesions first were m...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(89)90122-9
更新日期：1989-06-15 00:00:00
abstract：:A rapid method is described for the production of a human marrow cell suspension highly enriched in megakaryocytes. These concentrates were incubated with radiolabelled amino-acids, and cell lysates were then analysed for fibrinogen synthesis. Neosynthesized proteins were detected by immunoprecipitation, immuno-affini...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(85)90133-1
更新日期：1985-05-15 00:00:00
abstract：:The in vitro and in vivo pharmacological properties of two oversulfated dermatan sulfate (DS) derivatives, S-DS1 and S-DS2, containing 2 and 3.7 sulfate groups/disaccharide unit respectively were compared to those of the parent DS (1 sulfate group/disaccharide unit). In a purified system the ability of S-DS1 and of S-...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(88)90094-1
更新日期：1988-10-15 00:00:00
abstract：:Acute coronary syndromes (ACS) represent the main clinical manifestation of atherosclerotic progression in the coronary district. Thrombosis plays a critical role in the patho-anatomical of ACS, as disruption of an atherosclerotic plaque exposes flowing blood to subendothelial collagen, tissue factor, and other procoa...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2011.12.026
更新日期：2012-03-01 00:00:00
abstract：:We evaluate the anti-platelet and anti-thrombotic effects of cilostazol using Multiplate® and PFA-100® in vitro and ex vivo with freshly isolated rat whole blood and in vivo venous and arterial thrombosis models in the same species, in an effort to assess the sensitivity of the whole blood aggregometer assays without ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2011.02.004
更新日期：2011-06-01 00:00:00
abstract：INTRODUCTION:Conventional Vacutainer® Sodium Citrate blood collection tubes contain a relatively high concentration of contaminating magnesium ions, which may result in shortening of the prothrombin time (PT) and the International Normalized Ratio (INR). Recently the manufacturer of Vacutainer® Sodium Citrate tubes int...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2014.04.016
更新日期：2014-07-01 00:00:00
abstract：:We constructed a new factor IX expression vector containing a full length factor IX cDNA. The Moloney Murine Leukemia Virus (MoMLV)-based retroviral vector pLRNL was cloned with the entire coding region of human factor IX down stream of the promoter of Rous sarcoma virus, giving rise to the construct pL9RNL. The GP+E ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(93)90038-p
更新日期：1993-02-15 00:00:00
