Construction and its expression of a new retroviral vector containing a human blood coagulation factor IX cDNA.

abstract：INTRODUCTION:The contact system proteins factor XII (FXII), prekallikrein (PK) and high molecular weight kininogen (HK) have roles in coagulation, fibrinolysis, thrombin-induced platelet activation, cell adhesion and angeogenisis. It has been suggested that inherited or acquired deficiencies of these proteins may be ri...

journal_title：Thrombosis research

authors： Gallimore MJ,Harris SL,Jones DW,Winter M

更新日期：2004-01-01 00:00:00

abstract：:Data on the effect of hormone replacement therapy on hemostasis are inconsistent, and there are few data in women with coronary artery disease. In a single-center, open, randomized study, 118 postmenopausal women with angiographically verified coronary artery disease were randomized to hormone replacement therapy, giv...

journal_title：Thrombosis research

authors： Hoibraaten E,Os I,Seljeflot I,Andersen TO,Hofstad A,Sandset PM

更新日期：2000-04-01 00:00:00

abstract：:Plasma membranes of pig platelets were isolated and subjected to solubilization with digitonin. Binding of [3H]ketanserin to the solubilized membrane fraction, optimal at pH 7.5, was time and protein dependent and was greatly reduced by increasing ionic strength of the incubation buffer. Saturation analysis and inhibi...

journal_title：Thrombosis research

authors： Wesemann W,Hoffmann D

更新日期：1985-09-15 00:00:00

abstract：:Thrombin was acetylated by treatment with acetic anhydride, and the potency for protein C activation and the fibrinogen clotting activity of the resultant acetylthrombin were investigated in vitro and in vivo. The acetylthrombin retained an amidolytic activity to the synthetic substrate, S-2238, and reduced both of th...

journal_title：Thrombosis research

authors： Nakagomi K,Ajisaka K,Yokota I

更新日期：1990-08-15 00:00:00

abstract：BACKGROUND:The treatment of venous thromboembolism (VTE) in cancer patients remains controversial. PURPOSE:The Italian Society for Thrombosis and Haemostasis (SISET) commissioned a project to develop clinical practice guidelines for the therapy of VTE in patients with malignancies. METHODS:Key questions about the tre...

journal_title：Thrombosis research

authors： Imberti D,Di Nisio M,Donati MB,Falanga A,Ghirarduzzi A,Guarneri D,Piovella F,Santoro RC,Baldini E,Zampogna S,Italian Society for Thrombosis and Haemostasis.

更新日期：2009-11-01 00:00:00

abstract：:Although significant advances in hemophilia treatment have improved patient outcomes and quality of life, one of the greatest complications in severe hemophilia A is the development of anti-Factor VIII (FVIII) antibodies that inhibit FVIII activity in almost 30% of previously untreated patients (PUPs). Inhibitors make...

journal_title：Thrombosis research

authors： Garagiola I,Palla R,Peyvandi F

更新日期：2018-08-01 00:00:00

abstract：:Two functional assays for protein C inhibitor (PCI) were evaluated in parallel for the same plasma samples. One assay is specific for active PCI and measures its ability to form complexes with activated protein C (APC) in the presence of heparin (España et al, Thromb Res 64, 309, 1991) (Method A). After incubation of ...

journal_title：Thrombosis research

authors： España F,Hendl S,Gilabert J,Estellés A,Aznar J

更新日期：1993-06-01 00:00:00

abstract：:beta-Factor XIIa (beta-XIIa, Mr approximately 30,000) was isolated from human plasma by a procedure which utilized, as an initial step, the adsorption of Factor XII to celite. Activation of Factor XII and subsequent release of beta-XIIa was brought about by the proteolytic action of co-adsorbed kallikrein. Two success...

journal_title：Thrombosis research

authors： Tankersley DL,Alving BM,Finlayson JS

更新日期：1982-02-15 00:00:00

abstract：:Defibrotide both "in vitro" and "ex vivo" decreases PMN adhesion to nylon columns. This activity is blunted by 8-phenyltheophylline, a known blocker of adenosine A1/A2 receptors, suggesting that Defibrotide acts on that kind of receptors. Indomethacin, a blocker of PGI2 synthesis, did not cancel DFT's activity, sugges...

journal_title：Thrombosis research

authors： Alberico P,Porta R,Pescador R,Ferro L

更新日期：1995-11-15 00:00:00

abstract：:Fasting plasma homocysteine level and the related clinical findings were analysed in 240 consecutive patients with venous thromboembolism. Hyperhomocysteinemia, defined as a plasma level above 20 micromol/l (corresponding to the percentile 95th in the controls), was present in 11.2% of the patients. Plasma homocystein...

journal_title：Thrombosis research

authors： Hainaut P,Jaumotte C,Verhelst D,Wallemacq P,Gala JL,Lavenne E,Heusterspreute M,Zech F,Moriau M

更新日期：2002-04-15 00:00:00

abstract：:This study comprised 50 patients subjected to major abdominal surgery, of which 13 developed deep vein thrombosis (DVT) according to the 125I-fibrinogen test. Plasma was sampled preoperatively, for the specific analysis of tissue plasminogen activator (t-PA) before and during venous occlusion. The recently described f...

journal_title：Thrombosis research

authors： Mellbring G,Dahlgren S,Wiman B,Sunnegårdh O

更新日期：1985-07-15 00:00:00

abstract：:Understanding the intracellular events triggered by antiphospholipid (aPL) antibodies in platelets and endothelial cells (ECs) is important in designing new modalities of targeted therapies for the treatment of thrombosis in Antiphospholipid Syndrome (APS). A recent study showed a significant increase in the expressio...

journal_title：Thrombosis research

authors： Pierangeli SS,Vega-Ostertag M,Harris EN

更新日期：2004-01-01 00:00:00

abstract：BACKGROUND:Serious bleeding is a frequent and feared treatment complication in patients treated with oral anticoagulants (OACs). Levels of von Willebrand factor (VWF) antigen have been linked to the risk of bleeding complications, mortality, and cardiovascular events. OBJECTIVES:In this longitudinal cohort study of ev...

journal_title：Thrombosis research

authors： Lind M,Johansson L,Nilsson TK,Jansson JH,Hollestelle MJ

更新日期：2015-11-01 00:00:00

abstract：:Cell based models of coagulation (CBM) have provided mechanistic insight into numerous hematological issues for nearly two decades. This review discusses another coagulation model system--the clot lifespan model (CLSM)--that has been designed to compliment the CBM-based approach to elucidating the mechanisms responsib...

journal_title：Thrombosis research

authors： Nielsen VG

更新日期：2008-01-01 00:00:00

abstract：:Pulmonary embolism (PE) is a common, lethal yet treatable disease. The clinical diagnosis of PE remains to be a problem due to the nonspecific presenting signs, symptoms, electrocardiographic findings, arterial blood gas abnormalities and chest X-ray changes. Despite these nonspecific clinical findings, clinicians are...

journal_title：Thrombosis research

authors： Rodger M,Wells PS

更新日期：2001-09-15 00:00:00

abstract：:When a purified myeloma protein with the ability to inhibit fibrin polymerization was preincubated with sheep anti-human F(ab')2 antibodies, there was a marked reduction of the ability to prolong the thrombin clotting time, while anti-Fc antibodies had only a slight effect. A monoclonal immunoglobulin which shortens t...

journal_title：Thrombosis research

authors： Wisløff F,Michaelsen TE,Kierulf P,Godal HC

更新日期：1985-11-15 00:00:00

abstract：:The action of AD6 as an anti-thrombotic agent was studied in a model of coronary artery thrombosis and on platelet aggregation in the dog. AD6 (10-100 microM) in vitro inhibited aggregation induced by ADP, epinephrine, collagen and PAF (platelet aggregating factor) used at their threshold concentration for maximal agg...

journal_title：Thrombosis research

authors： Prosdocimi M,Finesso M,Tessari F,Gorio A,Languino LR,de Gaetano G,Dejana E

更新日期：1985-08-15 00:00:00

abstract：:Endothelial cell activation or damage is believed to play a key role in preeclampsia (PE) and may underlie the hemostatic changes observed in this syndrome. The aim of this study was to evaluate a relationship between maternal and cord blood hemostatic disturbances in preeclamptic pregnancies. We measured the plasma l...

journal_title：Thrombosis research

authors： Catarino C,Rebelo I,Belo L,Rocha S,Castro EB,Patricio B,Quintanilha A,Santos-Silva A

更新日期：2008-01-01 00:00:00

abstract：:The effects of platelets on clot lysis and plasminogen activation by staphylokinase (SAK) were investigated. At concentrations ranging from 2 x 10(-7) to 1 x 10(-5)g/ml of SAK, the lysis time of platelet-rich plasma clots (PRP-clots) was shorter than that of platelet-poor plasma clots (PPP-clots). This reduction of cl...

journal_title：Thrombosis research

authors： Suehiro A,Tsujioka H,Yoshimoto H,Ueda M,Higasa S,Kakishita E

更新日期：1995-10-15 00:00:00

abstract：:Four monoclonal antibodies have been produced, which are specific for the Ca2+ or Sr2(+)-induced conformation of human factor IX. Certain, but not all, gamma-carboxy-glutamic acid residues in factor IX are involved in the epitope expression together with the conformation stabilized by the adjacent region of Gla-domain...

journal_title：Thrombosis research

authors： Sugo T,Mizuguchi J,Kamikubo Y,Matsuda M

更新日期：1990-06-15 00:00:00

abstract：:The effect of dalteparin, a low molecular weight heparin, on severely antithrombin III (ATIII)-decreased disseminated intravascular coagulation (DIC) model was compared with that of unfractionated heparin (heparin). The DIC model in rabbits was produced by continuous infusion of thrombin in combination with bolus inje...

journal_title：Thrombosis research

authors： Tazawa S,Ichikawa K,Misawa K,Fukuyama J,Hamano S,Miyata H,Sakuragawa N

更新日期：1995-12-01 00:00:00

abstract：:Human platelets contain a single membrane glycoprotein which is susceptible to thrombin proteolysis, glycoprotein V. We have purified 1 mg of glycoprotein V from 10(13) platelets using a combination of gel filtration, hydroxylapatite and ion-exchange chromatographies. Glycoprotein V has a blocked amino-terminus. Follo...

journal_title：Thrombosis research

authors： Zafar RS,Walz DA

更新日期：1989-01-01 00:00:00

abstract：PURPOSE:By using endovascular techniques, we set up an animal model of mesenteric venous thrombosis to avoid surgical laparotomy. MATERIALS AND METHODS:Ten pigs underwent percutaneous transhepatic puncture to create animal model of acute superior mesenteric venous thrombosis. Experimental animals were injected with th...

journal_title：Thrombosis research

authors： Kai L,Jiaxiang M,Xinxin F,Baochen L,Weiwei D,Xingjiang W,Shuofei Y,Jieshou L

更新日期：2015-12-01 00:00:00

abstract：:The effects of extracellular sodium on platelet aggregation and calcium mobilization in platelets stimulated with either rattlesnake (Crotalus atrox) lectin (RSL) or alpha-thrombin were compared. The absence of extracellular sodium had no effect on platelet aggregation or calcium mobilization in response to all levels...

journal_title：Thrombosis research

authors： Wilson-Byl JA,Dockter ME,Gartner TK

更新日期：1991-11-01 00:00:00

abstract：:Platelet aggregation induced by ADP and collagen was monitored in 25 patients with increased serum levels of triglycerides (7 HLPs type IIb, 14 type IV, 4 type V patients) and compared with a group of 10 normolipidaemic control persons. Platelet aggregation was studied simultaneously in platelet rich plasma (PRP) by b...

journal_title：Thrombosis research

authors： Riess H,Merk W,Falkner C,Hiller E

更新日期：1986-02-01 00:00:00

abstract：OBJECTIVES:This study examines whether patient resistance to clopidogrel is associated with long-term thrombotic events after elective coronary drug-eluting stent (DES) implantation. METHODS:We prospectively enrolled 386 patients with stable angina who received elective percutaneous coronary intervention (PCI) with DE...

journal_title：Thrombosis research

authors： Wang ZJ,Zhou YJ,Liu YY,Yu M,Shi DM,Zhao YX,Guo YH,Cheng WJ,Jia de A,Cao Z,Nie B,Ge HL,Yang SW,Yan ZX

更新日期：2009-05-01 00:00:00

abstract：INTRODUCTION:Several hemostatic system components, including factor X (FX), contribute to cancer progression. The Protein Z (PZ)/protein Z-dependent protease inhibitor (ZPI) complex directly inhibits factor Xa proteolytic activity. The aim of this study was to determine the antigenic distribution of ZPI and PZ, in rela...

journal_title：Thrombosis research

authors： Sierko E,Wojtukiewicz MZ,Zimnoch L,Tokajuk P,Ostrowska-Cichocka K,Kisiel W

更新日期：2012-04-01 00:00:00

abstract：INTRODUCTION:Anedoctal reports suggest that some thrombotic primary antiphospholipid antibody syndrome (PAPS) patients on oral anticoagulation require higher than average doses to achieve given targets international normalized ratios (INR). MATERIALS AND METHODS:To test the hypothesis of relative warfarin resistance i...

journal_title：Thrombosis research

authors： Ames PR,Margaglione M,Ciampa A,Colaizzo D,Ferrara F,Iannaccone L,Vincenzobrancaccio

更新日期：2011-06-01 00:00:00

abstract：BACKGROUND:Cardiovascular mortality is especially low in southwest France (the French Paradox). In previous experimental studies, we found that alcohol-free extracts of armagnac could inhibit human platelet function in vitro and experimental thrombosis in vivo. To test the possible relevance of these findings, we teste...

journal_title：Thrombosis research

authors： Umar A,Depont F,Jacquet A,Lignot S,Segur MC,Boisseau M,Bégaud B,Moore N

更新日期：2005-01-01 00:00:00

abstract：:Platelet membrane glycoprotein V (GPV) was hydrolyzed during thrombin-induced platelet aggregation releasing a fragment GPVfl into the supernatant. Hydrolysis of GPV required catalytically active thrombin and was diminished by chemical modification of the fibrinogen binding site of thrombin. Half-maximal liberation of...

journal_title：Thrombosis research

authors： White GC 2nd,Knupp CL

更新日期：1985-06-15 00:00:00