当前位置： SCI文献检索 > THROMBOSIS RESEARCH期刊下所有文献 > Evaluation of anti-platelet and anti-thrombotic effects of cilostazol with PFA-100® and Multiplate® whole blood aggregometer in vitro, ex vivo and FeCl3-induced thrombosis models in vivo.

Evaluation of anti-platelet and anti-thrombotic effects of cilostazol with PFA-100® and Multiplate® whole blood aggregometer in vitro, ex vivo and FeCl3-induced thrombosis models in vivo.

Abstract：

:We evaluate the anti-platelet and anti-thrombotic effects of cilostazol using Multiplate® and PFA-100® in vitro and ex vivo with freshly isolated rat whole blood and in vivo venous and arterial thrombosis models in the same species, in an effort to assess the sensitivity of the whole blood aggregometer assays without potential issues of species differences. In vitro assay of anti-platelet effects of cilostazol against collagen-induced aggregation using Multiplate® produced a graded dose-dependent inhibition curve with IC50 value of 75.4 ± 2.4 μM while it showed a highly sensitive and all-or-none type inhibition response from 25 μM in PFA-100®. Interestingly, cilostazol manifested anti-thrombotic effects in vivo at much lower plasma concentrations than the effective concentrations measured in ex vivo or in vitro aggregation tests using PFA-100® or Multiplate®. In addition, the tail bleeding time measurement demonstrated that rats have lower sensitivity to the anti-platelet effects of cilostazol than mice. These results suggest that the detailed comparative evaluation of whole blood aggregometer assays with anti-thrombotic effects in vivo should be preceded before the application of these methods for the pharmacodynamic studies of anti-thrombotic agents.

journal_name

Thromb Res

journal_title

Thrombosis research

authors

Kim CW,Yun JW,Bae IH,Park YH,Jeong YS,Park JW,Chung JH,Park YH,Lim KM

doi

10.1016/j.thromres.2011.02.004

subject

Has Abstract

pub_date

2011-06-01 00:00:00

pages

565-70

issue

6

eissn

0049-3848

issn

1879-2472

pii

S0049-3848(11)00071-5

journal_volume

127

pub_type

杂志文章

相关文献

THROMBOSIS RESEARCH文献大全
  • Effects of lysophosphatidic acid on proliferation and cytosolic Ca++ of human adult vascular smooth muscle cells in culture.

    abstract：:Lysophosphatidic acid (LPA) is a lipid mediator generated by activated platelets and having various effects on numerous cell types. We investigated some effects of 1-oleyl LPA on vascular smooth muscle cells cultured from adult human normal arteries. At micromolar concentrations, LPA induced a mitogenic effect ([3H]-t...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/s0049-3848(99)00004-3

    authors： Gennero I,Xuereb JM,Simon MF,Girolami JP,Bascands JL,Chap H,Boneu B,Sié P

    更新日期：1999-06-01 00:00:00

  • Platelets as immune mediators: their role in host defense responses and sepsis.

    abstract：:Platelets occupy a central role at the interface between thrombosis and inflammation. At sites of vascular damage, adherent platelets physically and functionally interact with circulating leukocytes. Activated platelets release soluble factors into circulation that may have local and systemic effects on blood and vasc...

    journal_title：Thrombosis research

    pub_type： 杂志文章,评审

    doi：10.1016/j.thromres.2010.10.010

    authors： Li Z,Yang F,Dunn S,Gross AK,Smyth SS

    更新日期：2011-03-01 00:00:00

  • Postpartum blood transfusion and hemorrhage as independent risk factors for venous thromboembolism.

    abstract：INTRODUCTION:Profuse postpartum hemorrhage (PPH) and red blood cell (RBC) transfusion have been suggested to be associated with venous thromboembolic events (VTE). However, it is not fully clear if they are independent major risk factors. METHODS:Women who gave birth in the Stockholm area between 1999 and 2002 were th...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2018.03.002

    authors： Thurn L,Wikman A,Lindqvist PG

    更新日期：2018-05-01 00:00:00

  • Hemodynamics associated with atrial fibrillation directly alters thrombotic potential of endothelial cells.

    abstract：:An experimental in vitro model of the hemodynamics that occur in atrial fibrillation (AFib) in the left atrial appendage (LAA) was developed to study changes in human endothelial cell thrombotic potential. We applied human-derived sinus rhythm and AFib hemodynamic shear stress patterns to primary human endothelial cel...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2016.04.022

    authors： Simmers MB,Cole BK,Ogletree ML,Chen Z,Xu Y,Kong LJ,Mackman N,Blackman BR,Wamhoff BR

    更新日期：2016-07-01 00:00:00

  • Endoglycosidase H digestion of Yukb (Pena) alloantigen.

    abstract：:We characterized a platelet specific alloantigen (Yukb). In immunoblotting, anti-Yukb antibody was found to react with both 110 kDa and 96 kDa bands under nonreducing condition. Immunoblotting followed by separation by two-dimensional electrophoresis (isoelectric focusing/SDS-PAGE) showed that the 96 kDa band had a pI...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(92)90136-x

    authors： Sugano W,Ryo R,Yamaguchi N,Shibata Y

    更新日期：1992-07-15 00:00:00

  • Co-localization of Protein Z, Protein Z-Dependent protease inhibitor and coagulation factor X in human colon cancer tissue: implications for coagulation regulation on tumor cells.

    abstract：INTRODUCTION:Several hemostatic system components, including factor X (FX), contribute to cancer progression. The Protein Z (PZ)/protein Z-dependent protease inhibitor (ZPI) complex directly inhibits factor Xa proteolytic activity. The aim of this study was to determine the antigenic distribution of ZPI and PZ, in rela...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2011.10.027

    authors： Sierko E,Wojtukiewicz MZ,Zimnoch L,Tokajuk P,Ostrowska-Cichocka K,Kisiel W

    更新日期：2012-04-01 00:00:00

  • An inhibitor of activated thrombin-activatable fibrinolysis inhibitor potentiates tissue-type plasminogen activator-induced thrombolysis in a rabbit jugular vein thrombolysis model.

    abstract：:When activated in vitro, thrombin-activatable fibrinolysis inhibitor (TAFI) slows clot lysis by cleaving the C-terminal lysine and arginine residues from partially degraded fibrin. An inhibitor of carboxypeptidase isolated from potato (CPI) reverses prolongation of clot lysis by inhibiting activated TAFI. We investiga...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/s0049-3848(00)00184-5

    authors： Nagashima M,Werner M,Wang M,Zhao L,Light DR,Pagila R,Morser J,Verhallen P

    更新日期：2000-05-15 00:00:00

  • Thrombin reduces large heparan sulfate proteoglycan molecules in cultured vascular endothelial cell layers through inhibition of core protein synthesis.

    abstract：:We investigated the alteration of heparan sulfate proteoglycans induced by thrombin in cultured vascular endothelial cells. Heparan sulfate proteoglycans, which were metabolically labeled with [3H] glucosamine and [35S] sulfate, were isolated by DEAE-Sephacel ion-exchange chromatography and characterized by molecular ...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/s0049-3848(97)00257-0

    authors： Fujii N,Kaji T,Akai T,Koizumi F

    更新日期：1997-11-01 00:00:00

  • Subcutaneous treatment of deep venous thrombosis with low molecular weight heparin. A dose finding study with LMWH-Novo.

    abstract：:Treatment of deep venous thrombosis with low molecular weight heparin (LMWH-Novo, Logiparin) was carried out with two different doses of Logiparin, 75 XaI U/kg b.w. twice daily and 150 XaI U/kg b.w. once daily subcutaneously for 5 days. Simultaneously warfarin was given from the first day of heparin treatment. Mean ag...

    journal_title：Thrombosis research

    pub_type： 临床试验,杂志文章

    doi：10.1016/0049-3848(89)90307-1

    authors： Siegbahn A,Y-Hassan S,Boberg J,Bylund H,Neerstrand HS,Ostergaard P,Hedner U

    更新日期：1989-09-15 00:00:00

  • Tinzaparin in cancer associated thrombosis beyond 6months: TiCAT study.

    abstract：INTRODUCTION:The safety and efficacy of low-molecular-weight heparin (LMWH) treatment in patients with cancer-associated thrombosis (CAT) beyond 6months are unknown. Our aim was to determine the safety of long-term tinzaparin use in patients with CAT. METHODS:We performed a prospective, open, single arm, multicentre s...

    journal_title：Thrombosis research

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.thromres.2017.07.004

    authors： Jara-Palomares L,Solier-Lopez A,Elias-Hernandez T,Asensio-Cruz M,Blasco-Esquivias I,Marin-Barrera L,de la Borbolla-Artacho MR,Praena-Fernandez JM,Montero-Romero E,Navarro-Herrero S,Serrano-Gotarredona MP,Sánchez-Díaz JM,Palacios

    更新日期：2017-09-01 00:00:00

  • Circulating platelet-derived microparticles and endothelium-derived microparticles may be a potential cause of microthrombosis in patients with osteonecrosis of the femoral head.

    abstract：INTRODUCTION:To test the hypothesis that the platelet microparticle (PMP) and endothelial microparticle (EMP) may contribute to the hypercoagulability associated with microvascular thrombosis in patients with nontraumatc osteonecrosis of the femoral head (ONFH). MATERIALS AND METHODS:The study comprised 46 patients wh...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2008.04.001

    authors： Kang P,Shen B,Yang J,Pei F

    更新日期：2008-01-01 00:00:00

  • Anticoagulant effects of an antidiabetic drug on monocytes in vitro.

    abstract：INTRODUCTION:Monocyte- and microparticle (MP)-associated tissue factor (TF) is upregulated in diabetes. Lipopolysaccharide (LPS) induces expression of TF and alternatively spliced TF (asTF) and increases MP release from monocytes. Using LPS-stimulated TF-bearing human monocytes, we examined whether glibenclamide, a sul...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2011.07.007

    authors： Henriksson CE,Hellum M,Haug KB,Aass HC,Joø GB,Øvstebø R,Trøseid AM,Klingenberg O,Kierulf P

    更新日期：2011-11-01 00:00:00

  • Tissue factor activity is increased in a combined platelet and microparticle sample from cancer patients.

    abstract：BACKGROUND:Cancer patients have an increased risk of thrombosis. Tissue factor (TF) antigen and TF activity associated with microparticles in plasma are elevated in patients with various types of cancer. Of these two measurements, TF activity is considered superior to TF antigen levels because the activity more closely...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2007.12.023

    authors： Tilley RE,Holscher T,Belani R,Nieva J,Mackman N

    更新日期：2008-01-01 00:00:00

  • Physiological changes in membrane-expressed platelet factor 4: implications in heparin-induced thrombocytopenia.

    abstract：BACKGROUND:Many heparin-induced thrombocytopenia (HIT) antibodies cause platelet activation in the serotonin release assay (SRA) in the absence of heparin. This in vitro observation may help unravel the mechanism of delayed-onset HIT, where seropositive patients develop thrombocytopenia and associated thrombosis after ...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2009.10.021

    authors： Prechel MM,Jeske WP,Walenga JM

    更新日期：2010-04-01 00:00:00

  • Inhibitory effects of TFPI on thrombin and factor Xa generation in vitro--modulatory action of glycosaminoglycans.

    abstract：:The effect of tissue factor pathway inhibitor (TFPI) on thrombin and factor Xa generation was studied in an in vitro system using a prothrombin complex concentrate. It was found that TFPI, via the direct inhibition of factor Xa and the tissue factor/factor VIIa complex, inhibited both the further generation of factor ...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(94)90173-2

    authors： Kaiser B,Hoppensteadt DA,Jeske W,Wun TC,Fareed J

    更新日期：1994-09-15 00:00:00

  • Effects of tumor-expressed coagulation factors on cancer progression and venous thrombosis: is there a key factor?

    abstract：:Since the 19(th) century an association between cancer and thromboembolic events has been described, with a poorer survival prognosis. Production and secretion of procoagulant factors affect tumor biology and cancer-associated thrombosis. Tissue factor (TF) exerts coagulant and protease activated receptor (PAR)-depend...

    journal_title：Thrombosis research

    pub_type： 杂志文章,评审

    doi：10.1016/S0049-3848(14)50013-8

    authors： Ünlü B,Versteeg HH

    更新日期：2014-05-01 00:00:00

  • Identifying the factors influencing practice variation in thrombosis medicine: A qualitative content analysis of published practice-pattern surveys.

    abstract：:Practice variation, the differences in clinical management between physicians, is one reason why patient outcomes may differ. Identifying factors that contribute to practice variation in areas of clinical uncertainty or equipoise may have implications for understanding and improving patient care. To discern what facto...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2017.09.026

    authors： Skeith L,Gonsalves C

    更新日期：2017-11-01 00:00:00

  • Activation contact system (ACS) and tissue factor (TF) in human amniotic fluid: measurements of ACS components and TF, and some implications on the pathophysiology of amniotic fluid embolism.

    abstract：BACKGROUND/AIM:It is believed that the amniotic fluid-derived TF, in the case of amniotic fluid embolism (AFE), contributes to acute disseminated intravascular coagulation (DIC) and obstetric shock in the mother. However, the role of amniotic fluid-derived contact phase coagulation factors that irrupt into the bloodstr...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2014.10.014

    authors： Uszyński W,Żekanowska E,Uszyński M,Kieszkowski P

    更新日期：2015-04-01 00:00:00

  • A human factor VIIa concentrate and its effects in the hemophilic A dog.

    abstract：:Properties of a new concentrate of FVII/FVIIa, obtained by adsorption onto an inorganic adsorbent followed by a chromatographic step onto Q-Sepharose using a by-product of routine fractionation as starting material, are described. This fraction contains only small amounts of the other components of the prothrombin com...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(89)90126-6

    authors： Chabbat J,Hampikian-Lenin S,Toully V,Gaillandre A,Péjaudier L,Steinbuch M

    更新日期：1989-06-15 00:00:00

  • Amino acid substitutions of the P2 residue of human antithrombin that either enhance or impair function.

    abstract：:Recombinant forms of human antithrombin (AT) were expressed in COS-1 cells, and their interaction with human thrombin characterized by comparing the reactivity of two engineered mutant forms of AT with the wild-type recombinant. Both mutant forms contained single amino acid substitutions of Asp (G392D) or Pro (G392P) ...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(94)90240-2

    authors： Sheffield WP,Blajchman MA

    更新日期：1994-08-01 00:00:00

  • Knockdown of liver-derived factor XII by GalNAc-siRNA ALN-F12 prevents thrombosis in mice without impacting hemostatic function.

    abstract：BACKGROUND:Plasma coagulation Factor XII (FXII) plays a crucial role in contact activation, ultimately regulating both the kinin-kallikrein system and the intrinsic pathway of coagulation. A growing body of evidence suggests that inhibition of FXII can prevent thrombosis. Given FXII does not appear to modulate hemostas...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2020.08.040

    authors： Liu J,Cooley BC,Akinc A,Butler J,Borodovsky A

    更新日期：2020-12-01 00:00:00

  • "HERDOO2" clinical decision rule to guide duration of anticoagulation in women with unprovoked venous thromboembolism. Can I use any d-Dimer?

    abstract：BACKGROUND:The "HERDOO2 rule" is a prospectively validated clinical decision rule used to identify low-risk women who can safely discontinue anticoagulants after completing 5-12 months of anticoagulant treatment for unprovoked venous thromboembolism. The VIDAS®d-Dimer (DD) assay, a component of the rule, was used in th...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2018.07.020

    authors： Rodger MA,Le Gal G,Langlois NJ,Gin B,Mallick R,Giulivi A,Freedman M,Kovacs MJ,REVERSE II investigators.

    更新日期：2018-09-01 00:00:00

  • Effect of thromboxane synthase inhibition on the thrombolytic action of tissue-type plasminogen activator in a rabbit model of peripheral arterial thrombosis.

    abstract：:The thrombolytic efficacy of recombinant tissue-type plasminogen activator (tPA) in the presence and absence of a thromboxane synthase inhibitor was studied in a model of femoral artery thrombosis in the anesthetized rabbit. The thrombus was formed by injection of thrombin and whole blood into an isolated segment of t...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(88)90022-9

    authors： Shebuski RJ,Storer BL,Fujita T

    更新日期：1988-12-01 00:00:00

  • Growth and function of the normal human placenta.

    abstract：:The placenta is the highly specialised organ of pregnancy that supports the normal growth and development of the fetus. Growth and function of the placenta are precisely regulated and coordinated to ensure the exchange of nutrients and waste products between the maternal and fetal circulatory systems operates at maxim...

    journal_title：Thrombosis research

    pub_type： 杂志文章,评审

    doi：10.1016/j.thromres.2004.06.038

    authors： Gude NM,Roberts CT,Kalionis B,King RG

    更新日期：2004-01-01 00:00:00

  • Analysis of serum cancer procoagulant activity and its possible use as a tumor marker.

    abstract：:The two objectives of the study were to determine whether a procedure could be developed for measuring cancer procoagulant (CP) activity in human serum and if this procedure provided a method for distinguishing people with cancer from those without cancer. A procedure was developed for processing human serum such that...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(89)90256-9

    authors： Gordon SG,Benson B

    更新日期：1989-11-01 00:00:00

  • Risk of early recurrent fetal loss and levels of thrombin-activatable fibrinolysis inhibitor.

    abstract：INTRODUCTION:Though thrombin-activatable fibrinolysis inhibitor (TAFI) may contribute to hypercoagulability during pregnancy, limited data are available on the role of TAFI in women with recurrent fetal loss. MATERIAL/METHODS:We performed a case-control study aimed at evaluating any possible association between TAFI l...

    journal_title：Thrombosis research

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.thromres.2011.09.029

    authors： Legnani C,Bovara M,Valdrè L,Cosmi B,Caniato A,Palareti G

    更新日期：2012-08-01 00:00:00

  • Clinical study of optical coherence tomography in the diagnosis of peripheral pulmonary artery thrombus.

    abstract：BACKGROUND:Peripheral pulmonary artery thrombus (PPT) is common in the clinic. However, due to the lack of an ideal diagnostic tool, PPT cannot be quickly diagnosed and effectively treated at present. Optical coherence tomography (OCT) is a new intravascular imaging technique that is characterized by high image resolut...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2017.10.021

    authors： Hong C,Luo FQ,Liu CL,Zhong NS,Li JY,Wang W

    更新日期：2018-01-01 00:00:00

  • Development of an ELISA for the quantification of fibrin in canine tumours.

    abstract：:Fibrin is found in most solid tumours, and there is speculation regarding its role in tumour invasion and metastasis. An assay to quantitate fibrin levels in tissues would be a useful preliminary tool in assessing the above. Such an assay to quantitatively detect levels of fibrin in various types of canine tumour was ...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/s0049-3848(99)00054-7

    authors： Raut S,McEvoy F,Gaffney PJ

    更新日期：1999-10-01 00:00:00

  • Fluorescent labeling of blood platelets in vivo.

    abstract：:In rabbits, a clear microscopic image of individual, fluorescent blood platelets flowing in a mesenteric microvessel could be obtained over the whole cross-sectional area of the vessel, following an intravenous injection of a solution containing 5-15 mg of the fluorochrome Acridine Red. Most of the circulating platele...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(82)90106-2

    authors： Tangelder GJ,Slaaf DW,Reneman RS

    更新日期：1982-12-15 00:00:00

  • Endogenous forms of fibrinogen in Hep G2 cells.

    abstract：:The three polypeptide chains of fibrinogen, A alpha, B beta and gamma chain, are synthesized on separate polysomes. Fully formed fibrinogen is a six chain, disulfide-linked, dimeric molecule with a molecular weight of 340kDa. Previous pulse-chase studies with L-35 S methionine using the human hepatocellular carcinoma ...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(87)90290-8

    authors： Yu S,Kong V,Kudryk B,Redman C

    更新日期：1987-04-15 00:00:00