Abstract：
INTRODUCTION:Monocyte- and microparticle (MP)-associated tissue factor (TF) is upregulated in diabetes. Lipopolysaccharide (LPS) induces expression of TF and alternatively spliced TF (asTF) and increases MP release from monocytes. Using LPS-stimulated TF-bearing human monocytes, we examined whether glibenclamide, a sulfonylurea used to treat diabetes type 2, might possess anticoagulant properties. METHODS:We studied the effects of glibenclamide on cell- and supernatant-associated procoagulant activity (Factor Xa-generating assay and clot formation assay), on expression of TF and asTF (flow cytometry, RT-qPCR, western blot) and on cell viability and MP release (flow cytometry). RESULTS:Glibenclamide dose-dependently decreased procoagulant activity of cells and supernatants. The reduction in cellular procoagulant activity coincided with reduced expression of TF and asTF in cells, whereas cell viability remained almost unchanged. The glibenclamide-induced reduction in procoagulant activity of supernatants appeared to be associated with a decreased number of released MPs. CONCLUSIONS:Reduction of monocyte- and supernatant-associated procoagulant activity by glibenclamide is associated with decreased expression of TF and asTF and possibly with a reduced MP number. Our data indicate that glibenclamide reduces the prothrombotic state in LPS-stimulated monocytes in vitro. Glibenclamide might therefore also have an anticoagulant effect in vivo, but this needs to be further evaluated.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Henriksson CE,Hellum M,Haug KB,Aass HC,Joø GB,Øvstebø R,Trøseid AM,Klingenberg O,Kierulf Pdoi
10.1016/j.thromres.2011.07.007subject
Has Abstractpub_date
2011-11-01 00:00:00pages
e100-6issue
5eissn
0049-3848issn
1879-2472pii
S0049-3848(11)00353-7journal_volume
128pub_type
杂志文章abstract：BACKGROUND AND OBJECTIVE:According to US Food and Drugs Administration (FDA), 2 hour recombinant tissue plasminogen activator (rt-PA) 100mg infusion is recommended for eligible patients with acute pulmonary embolism (PE). However,there exists evidence implying that a lower dosage of rt-PA can be equally effective but p...
journal_title：Thrombosis research
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.thromres.2013.12.026
更新日期：2014-03-01 00:00:00
abstract：INTRODUCTION:Thrombosis and coagulation abnormalities are thought to be involved in disease progression of pulmonary hypertension. Platelet-derived microparticles (PDMP) are released from platelets following stimulation and have recently been demonstrated to play an important role in pathogenesis of various diseases. T...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2020.07.030
更新日期：2020-11-01 00:00:00
abstract：INTRODUCTION:Hip fracture is common among the elderly and is associated with increased morbidity and mortality, particularly when surgery is delayed. Direct oral anticoagulants (DOACs) use might increase bleeding and postpone hip repair surgery. We aimed to assess the association between preoperative DOACs use and adve...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2020.03.002
更新日期：2020-05-01 00:00:00
abstract：:Patients with the nephrotic syndrome are at increased risk of developing venous and arterial thromboembolism, the most common of which is renal vein thrombosis. There are several unanswered or controversial issues relating to the nephrotic syndrome and thromboembolism, which include the mechanism of thromboembolism, a...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2005.03.030
更新日期：2006-01-01 00:00:00
abstract：:Activated factor X (FXa) and thrombin can up-regulate gene expression of connective tissue growth factor (CTGF/CCN2) on fibroblasts. Since tissue factor (TF) is expressed on these cells, we hypothesized that they may assemble the prothrombinase complex leading to CTGF/CCN2 upregulation. In addition, the effect of thro...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2011.08.009
更新日期：2012-06-01 00:00:00
abstract：:Low circulating protein C (PC) levels have been observed in sepsis, especially in patients with Neisseriae Meningitides infections. Poor clinical outcome and high limb amputation rates have been associated in infected patients with low circulating PC levels. Published studies using activated PC replacement therapy pat...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2014.04.019
更新日期：2014-07-01 00:00:00
abstract：:The susceptibility of von Willebrand factor /vWF/ to digestion by human neutrophil cathepsin G in highly purified FVIII/vWF concentrate /BHS/ and in cryoprecipitate has been studied. In contrast to human neutrophil elastase, cathepsin G inactivates and degrades vWF not only in BHS but also in cryoprecipitate. Fibrinog...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(91)90657-i
更新日期：1991-02-15 00:00:00
abstract：INTRODUCTION:Tumor necrosis factor-alpha (TNF-α) exerts a critical role in inflammatory events through two distinct receptors, TNFR1 and TNFR2. Platelets have been recognized as important inflammatory cells, but little is known about the effects of TNF-α on the platelet activity. OBJECTIVES:In the present study we hav...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2018.10.008
更新日期：2018-12-01 00:00:00
abstract：:Previous studies have indicated that the skin bleeding time test does not accurately reflect visceral bleeding time (BT). The present study examines the predictive value of the skin bleeding time for brain bleeding tendency. Sixteen Sprague-Dawley rats were divided into equal groups. The first group (controls) underwe...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(94)90282-8
更新日期：1994-12-15 00:00:00
abstract：:ZK-807834 (also known as CI-1031) is a small molecule that potently and selectively inhibits Factor Xa. Studies in animals have shown that ZK-807834 attenuates thrombosis and thrombus progression after fibrinolysis and exhibits an increased antithrombotic-to-bleeding risk ratio compared with conventional agents. The p...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(02)00024-5
更新日期：2002-02-15 00:00:00
abstract：:Generation of active Factor XII (FXIIa) triggers blood clotting on artificial surfaces and may also enhance intravascular thrombosis. We developed a patterned kaolin (0 to 0.3 pg/μm(2))/type 1 collagen fibril surface for controlled microfluidic clotting assays. Perfusion of whole blood (treated only with a low level o...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2014.09.030
更新日期：2014-12-01 00:00:00
abstract：INTRODUCTION:Normal pregnancy is associated with hypercoagulable state. Elevated markers of coagulation and fibrinolytic system activation indicate increased thrombin activity and increased fibrinolysis following fibrin formation throughout pregnancy. These changes exceed the biological variability in most cases. Haemo...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2013.02.005
更新日期：2013-04-01 00:00:00
abstract：:Anticoagulant fucoidan fractions of different molecular weight and sulfate content were prepared and investigated for their effects on platelet function in vitro. The fucoidan fractions were incubated with human platelet rich plasma (PRP) at concentrations of 5, 10 and 50 micrograms/ml. Platelet activation was subsequ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(97)00037-6
更新日期：1997-03-15 00:00:00
abstract：BACKGROUND:Use of antiplatelet therapy in combination with oral anticoagulants remains controversial. The purpose of this article is to review current consensus recommendations for antithrombotic therapy, to evaluate risks for bleeding among patients taking combination antithrombotic therapy, and lastly to review singl...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2008.08.011
更新日期：2008-01-01 00:00:00
abstract：:Isolated rat liver macrophages (Kupffer cells) produce a factor(s) which stimulates fibrinogen synthesis 5-8 fold in hepatocyte cultures. The production of the stimulatory factor reached maximum during the first 24 hours the cells were in culture. When the cell culture medium was chromatographed on a calibrated Sephad...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(83)90025-7
更新日期：1983-10-15 00:00:00
abstract：INTRODUCTION:Venous thromboembolism is a common cause of morbidity and mortality. Although cirrhosis has classically been considered as an acquired bleeding diathesis, there is increasing evidence that rejects the traditional belief that these patients are naturally protected against venous thromboembolism. However, an...
journal_title：Thrombosis research
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.thromres.2013.08.001
更新日期：2013-10-01 00:00:00
abstract：INTRODUCTION:Inter-individual variability in clopidogrel responsiveness is dependent on genetic polymorphisms. We aimed to investigate the impact of 3 genetic polymorphisms involved in clopidogrel metabolism on a strategy of dose-adjustment according to platelet reactivity (PR) monitoring. MATERIAL AND METHODS:[correc...
journal_title：Thrombosis research
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1016/j.thromres.2011.12.038
更新日期：2012-07-01 00:00:00
abstract：BACKGROUND:Clinical and epidemiological studies have associated selective COX-2 inhibitors with an increased risk of cardiovascular events. There are no clinical studies on the possible effects of these drugs on secondary hemostasis. The hypothesis for this study is that the use of selective COX-2 inhibitors could affe...
journal_title：Thrombosis research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/j.thromres.2009.01.017
更新日期：2009-06-01 00:00:00
abstract：INTRODUCTION:Patients with stable coronary artery disease (CAD) are at risk of arterial thrombosis causing myocardial infarction. Detection of global haemostatic markers of hypercoagulability and hypofibrinolysis may be important for risk stratification and individualised treatment. We examined overall haemostatic pote...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2013.03.015
更新日期：2013-05-01 00:00:00
abstract：:To compare Canola a terrestrial (n-3) oil with fish oil (n-3) and common vegetable oil (n-6) in decreasing serum lipids and platelet aggregation, various oils were included in Chow-based diets and fed to rats in an eight-weeks feeding trial. The Chow diet contained 2% cholesterol along with either Canola, menhaden, sa...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(90)90420-h
更新日期：1990-08-01 00:00:00
abstract：:We previously reported that the expression of thrombomodulin on the MEG-01, a cell line from human megakaryoblastic leukemia, was increased by agents that increase intracellular cAMP. In this paper we examine the effect of these agents on cultured human umbilical vein endothelial cells (HUVEC) and mouse hemangioma cel...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(91)90107-8
更新日期：1991-02-01 00:00:00
abstract：:Antithrombin (AT) is a serpin capable of trapping thrombin (IIa) in a stable and covalent complex. Complex formation is prevented by leukocyte elastase (LE) cleavage near the AT reactive centre. We mutated the known LE cleavage sites of AT to explore the possibility of producing an LE-resistant AT molecule. Initially,...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(97)00228-4
更新日期：1997-10-15 00:00:00
abstract：:Amino sugars and basic amino acids inhibit platelet aggregation and the activity of the endogenous platelet lectin, yet, relatively high concentrations (approximately 30 mM) are required for the inhibition. If cooperative interactions are involved in these platelet surface activities, oligomers of primary amines shoul...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(84)90160-9
更新日期：1984-02-01 00:00:00
abstract：INTRODUCTION:Profuse postpartum hemorrhage (PPH) and red blood cell (RBC) transfusion have been suggested to be associated with venous thromboembolic events (VTE). However, it is not fully clear if they are independent major risk factors. METHODS:Women who gave birth in the Stockholm area between 1999 and 2002 were th...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2018.03.002
更新日期：2018-05-01 00:00:00
abstract：BACKGROUND:The AMPLIFY trial found significantly lower major bleeding (MB) and similar recurrent venous thromboembolism (VTE) risks associated with apixaban vs warfarin among patients with VTE. OBJECTIVES:To compare MB, clinically-relevant non-major (CRNM) bleeding, and recurrent VTE risks among clinically-relevant su...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2020.11.039
更新日期：2020-12-08 00:00:00
abstract：:A Ca++-ionophore, ionomycin, increased the volume of human platelets suspended in a Ca++-containing buffer. This change in cell volume was dependent upon ionomycin and extracellular Ca++ concentrations, suggesting that the volume change occurs when the intracellular Ca++ reaches a certain level (greater than uM as det...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(90)90197-k
更新日期：1990-01-01 00:00:00
abstract：:Because of a thrombasthenia-like platelet aggregation defect, platelets from dogs affected with Basset Hound Hereditary Thrombopathy were compared to normal control dog platelets by three different techniques in order to assess platelet membrane glycoprotein content. Crossed immunoelectrophoresis (CIE), two-dimensiona...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(86)90295-1
更新日期：1986-04-15 00:00:00
abstract：:Murine monoclonal antibodies (mAb) were raised to a purified product of bovine PF-4, a 9,500 dalton protein with heparin neutralization activity comparable to that of human PF-4. Using a non-radioactive slide immunoenzymatic assay, four major classes of mAb could be identified when comparisons were made between purifi...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(86)90384-1
更新日期：1986-03-15 00:00:00
abstract：INTRODUCTION:High concentrations of N-terminal-pro-brain natriuretic peptide (NT-proBNP) are found in patients with right ventricular overload. Right ventricular overload may be the result of large perfusion defects in patients with pulmonary embolism (PE). NT-proBNP levels are associated with poorer outcome in patient...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2011.02.014
更新日期：2011-06-01 00:00:00
abstract：:Four novel polymorphisms were identified in the fibrinogen gene cluster. Three of them were localized in the promoter regions of the Aalpha-chain (alpha -128 C/G, alpha -58 G/A) or the gamma-chain (gamma -239 A/G) gene, while the remaining one was identified in intron 9 of the gamma-chain gene (gamma 7792 C/T). Genoty...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(01)00329-2
更新日期：2001-08-15 00:00:00
