Abstract:
BACKGROUND:Cancer patients have an increased risk of thrombosis. Tissue factor (TF) antigen and TF activity associated with microparticles in plasma are elevated in patients with various types of cancer. Of these two measurements, TF activity is considered superior to TF antigen levels because the activity more closely reflects the ability of TF to initiate coagulation. Recent studies showed that platelets also express TF. OBJECTIVE:To determine the level of TF activity associated with a combined platelet and microparticle sample from cancer patients (n = 20) and healthy individuals (n = 23). METHODS:TF activity was measured using a two step chromogenic assay and soluble P-selectin was measured by ELISA in healthy controls and metastatic cancer patients. RESULTS:We determined the composition of a combined platelet and microparticle sample. The sample consisted of platelets, large microparticles (30-200 nm) and membrane debris. We compared the TF activity of a combined platelet and microparticle sample from cancer patients with that from healthy individuals. We found that TF activity in a combined platelet and microparticle sample from cancer patients was higher than in samples from healthy individuals (21.5+/-12.3 pM (n = 20) versus 8.6+/-6.8 pM (n = 23), mean+/-SD, p < 0.001). Cancer patients also had a higher level of soluble P-selectin compared with controls (18.9+/-5.5 ng/mL versus 13.2+/-2.3 ng/mL, p < 0.001). CONCLUSION:This study indicates that measurement of TF activity in a combined platelet and microparticle sample can be used as a simple assay to determine the level of circulating TF.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Tilley RE,Holscher T,Belani R,Nieva J,Mackman Ndoi
10.1016/j.thromres.2007.12.023subject
Has Abstractpub_date
2008-01-01 00:00:00pages
604-9issue
5eissn
0049-3848issn
1879-2472pii
S0049-3848(08)00003-0journal_volume
122pub_type
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pub_type: 杂志文章,评审
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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pub_type: 临床试验,杂志文章
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更新日期:2015-09-01 00:00:00
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journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2006.12.004
更新日期:2007-01-01 00:00:00
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pub_type: 杂志文章,meta分析,评审
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更新日期:2003-06-15 00:00:00
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更新日期:2018-08-01 00:00:00
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pub_type: 杂志文章,多中心研究
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abstract:INTRODUCTION:Increased levels of circulating tissue factor (TF)-positive microvesicles (MVs) may increase the risk of thrombosis. Indeed, TF-positive MVs are detected in plasma of patients with various types of diseases. In this study, we measured levels of MV TF activity in non-cancer severely ill patients and cancer ...
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pub_type: 杂志文章
doi:10.1016/j.thromres.2018.03.001
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pub_type: 杂志文章
doi:10.1016/0049-3848(87)90074-0
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pub_type: 杂志文章
doi:10.1016/j.thromres.2012.05.005
更新日期:2012-09-01 00:00:00
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doi:10.1016/j.thromres.2006.06.014
更新日期:2007-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2002-09-15 00:00:00
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journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2006.06.005
更新日期:2007-01-01 00:00:00
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doi:10.1016/j.thromres.2018.11.003
更新日期:2018-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0049-3848(87)90236-2
更新日期:1987-07-01 00:00:00
abstract::Several single-nucleotide polymorphisms (SNPs) have been recognized as associated with ischemic heart disease (IHD) although the optimal set of risk genotypes has not be identified. This study aimed to examine whether identified high-risk SNPs are associated with early onset of IHD. In the GENOCOR study, 44 high-risk ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2014.03.006
更新日期:2014-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.thromres.2014.11.016
更新日期:2015-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.thromres.2017.08.001
更新日期:2017-10-01 00:00:00
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journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(88)90257-5
更新日期:1988-03-15 00:00:00
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pub_type: 杂志文章
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更新日期:2001-05-01 00:00:00