Abstract：
BACKGROUND:Whether antibodies directed to β2-Glycoprotein I (aβ2GPI) are responsible for LA activity is not well defined. However, in the absence of such antibodies the molecule responsible for LA phenomenon is unknown. OBJECTIVE:The aim of this study was the biochemical identification of the target antigen epitope of aPL responsible of LA activity in the absence of aβ2GPI antibodies together with the biological and clinical characteristics of these patients in comparison with classical triple positive patients. PATIENTS/METHODS:A comparison of patients with LA without (LA+/aβ2GPI-) and those with (LA+/aβ2GPI+) associated aβ2GPI antibodies was performed. Size exclusion chromatography and analytical chromatography were used to identify the molecule with LA activity in patients LA+/aβ2GPI-. RESULTS AND CONCLUSIONS:Analytical size-exclusion chromatography revealed a peak of 996Kd with LA activity perfectly overlapping that of IgM anti phosphatidylserine/prothrombin (aPS/PT) antibodies. Similarly, all the 25 LA+/aβ2GPI- patients were positive for aPS/PT antibodies. LA+/aβ2GPI- compared to 33 LA+/aβ2GPI+ patients turned out to be significantly older, with a lower rate of previous thromboembolic events and a weaker LA activity. Search for aPS/PT and aβ2GPI antibodies in patients with LA is useful to identify two subgroups of LA at different risk of thromboembolic events.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Pengo V,Del Ross T,Ruffatti A,Bison E,Cattini MG,Pontara E,Testa S,Legnani C,Pozzi N,Peterle D,Acquasaliente L,De Filippis V,Denas Gdoi
10.1016/j.thromres.2018.11.003subject
Has Abstractpub_date
2018-12-01 00:00:00pages
172-178eissn
0049-3848issn
1879-2472pii
S0049-3848(18)30597-8journal_volume
172pub_type
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journal_title：Thrombosis research
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journal_title：Thrombosis research
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journal_title：Thrombosis research
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pub_type： 杂志文章
doi：10.1016/0049-3848(83)90181-0
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pub_type： 杂志文章
doi：10.1016/0049-3848(85)90209-9
更新日期：1985-06-15 00:00:00
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pub_type： 杂志文章,评审
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pub_type： 临床试验,杂志文章,随机对照试验
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