First visualization of circulating neutrophil extracellular traps using cell fluorescence during human septic shock-induced disseminated intravascular coagulation.

Abstract:

:Disseminated intravascular coagulation (DIC) is a severe complication of septic shock. Polymorphonuclear neutrophils (PMNs) may play a key role in septic shock-induced DIC via the release of neutrophils extracellular traps (NETs). NETs capture invading pathogens, but also act as a pro-coagulant surface at the interface between immunity and thrombosis. During septic shock-induced DIC, neutrophil activation may result in excessive NET formation. Herein, we originally report the presence of circulating NETs in human blood during septic shock-induced DIC. To investigate NET formation during shock-induced DIC neutrophils were isolated from patients in septic shock associated with (n = 3) or without (n = 3) DIC. Neutrophils from healthy donors (n = 3) were stimulated in vitro with ionomycin as NET formation positive controls. PMNs smears were stained with mouse anti-human FITC anti-myeloperoxidase antibody and the blue-fluorescent DAPI nucleic acid stain. NETs were identified as elongated extracellular DNA fibers associated to myeloperoxidase detected by immunofluorescence. NETs were unambiguously observed in PMNs from septic shock patients with DIC but not from patients without DIC. NETs features in DIC+ patients were undistinguishable from those observed in ionomycin-induced PMNs from healthy donors. Fluorescence images of NETs were associated to extracellular cytoplasmic expansions. Our data report for the first time the direct visualization of circulating NETs in patients with septic shock-induced DIC. The in vivo relevance of previously reported indirect markers of NETosis (neutrophil side fluorescence) is confirmed.

journal_name

Thromb Res

journal_title

Thrombosis research

authors

Stiel L,Mayeur-Rousse C,Helms J,Meziani F,Mauvieux L

doi

10.1016/j.thromres.2019.09.036

subject

Has Abstract

pub_date

2019-11-01 00:00:00

pages

153-158

eissn

0049-3848

issn

1879-2472

pii

S0049-3848(19)30438-4

journal_volume

183

pub_type

杂志文章
  • Dexamethasone increases the release of three 44 kD proteins immunologically related to plasminogen activator inhibitor-1 from human umbilical vein endothelial and rabbit coronary microvessel endothelial cells.

    abstract::Endothelial cells synthesize and release different proteins involved in their function, and some of these proteins may play important roles in the cellular response to injury, infection, or glucocorticoids. We have examined the profile of proteins released from rabbit coronary microvascular endothelial (RCME) and huma...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/0049-3848(90)90202-n

    authors: Partridge CA,Gerritsen ME

    更新日期:1990-01-01 00:00:00

  • Endothelial dysfunction, nitric oxide and platelet activation in hypertensive and diabetic type II patients.

    abstract::Alterations in the synthesis or enhanced inactivation of nitric oxide (NO) and an increase in endothelin-1 production lead to an imbalance that can induce arterial hypertension. Type II diabetes is characterized by impaired endothelium-dependent vasodilation and vascular disease. NO is produced through L-arginine path...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/s0049-3848(01)00237-7

    authors: Ouviña SM,La Greca RD,Zanaro NL,Palmer L,Sassetti B

    更新日期:2001-04-15 00:00:00

  • Response of protein C and protein C inhibitor to warfarin therapy in patient with combined deficiency of Factors V and VIII.

    abstract::The role of Protein C in combined factor V/VIII deficiency was examined by reducing the Protein C concentration using warfarin therapy in a patient with the combined deficiency. The factor VIII deficiency was like Hemophilia-A, with deficiency of VIII:C and VIII:C(Ag), but normal VIIIR:Ag and VIIIR:cof. The factor V d...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/0049-3848(84)90188-9

    authors: Bern MM,Suzuki K,Mann K,Tracy P,Hoyer L,Jensen W,Gallivan M,Arkin C,Davis G

    更新日期:1984-12-15 00:00:00

  • Thromboembolism and anticoagulant management in hemodialysis patients: a practical guide to clinical management.

    abstract::The need for anticoagulation in dialysis patients is common and the incidence of venous thromboembolism (VTE) and atrial fibrillation in this population is high. While direct data are lacking on the management of anticoagulation in dialysis patients, careful weighing of risks and benefits on the basis of evidence from...

    journal_title:Thrombosis research

    pub_type: 杂志文章,实务指引

    doi:10.1016/j.thromres.2005.03.031

    authors: Lo DS,Rabbat CG,Clase CM

    更新日期:2006-01-01 00:00:00

  • Changes of the fibrinolytic system of animals induced by injection of tissue plasminogen activator.

    abstract::The changes in the fibrinolytic system of rats induced by repeated injections of tissue plasminogen activator (TPA) have been studied. The euglobulin fraction fibrinolytic activity increases 10 min after the first injection of TPA. Repeated injections of TPA for one or more days do not stimulate the fibrinolytic activ...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/0049-3848(82)90075-5

    authors: Andreenko GV,Lyutova LV,Shimonaeva EE

    更新日期:1982-08-01 00:00:00

  • Platelet activity is negatively modulated by tumor necrosis factor alpha through reductions of cytosolic calcium levels and integrin alphaIIbbeta3 phosphorylation.

    abstract:INTRODUCTION:Tumor necrosis factor-alpha (TNF-α) exerts a critical role in inflammatory events through two distinct receptors, TNFR1 and TNFR2. Platelets have been recognized as important inflammatory cells, but little is known about the effects of TNF-α on the platelet activity. OBJECTIVES:In the present study we hav...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/j.thromres.2018.10.008

    authors: Bonfitto PHL,Naime ACA,Lopes-Pires ME,Goulart G,Mendes-Silverio CB,Bueno PI,Castilho RF,Antunes E,Marcondes S

    更新日期:2018-12-01 00:00:00

  • Reactivity of heparin with the human plasma heparin-binding proteins thrombin, antithrombin III, and apolipoproteins E and B-100.

    abstract::The heparin-binding properties of human plasma apolipoproteins B-100 and E (apoB-100 and E) of low density lipoproteins (LDL), thrombin, and antithrombin-III (AT-III) were investigated. A highly reactive heparin (HRH) to apoB-100 was isolated by chromatography of crude heparin on a column of LDL immobilized to Affi-Ge...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/0049-3848(84)90258-5

    authors: Cardin AD,Barnhart RL,Witt KR,Jackson RL

    更新日期:1984-06-15 00:00:00

  • Abnormal Ca2+ homeostasis in platelets of patients with myeloproliferative disorders: low levels of Ca2+ influx and efflux across the plasma membrane and increased Ca2+ accumulation into the dense tubular system.

    abstract::Ca2+ influx and efflux in unstimulated platelets and Ca2+ uptake by simultaneously isolated two membrane fractions of platelets from patients with myeloproliferative disorders (MPD) have been investigated. In MPD, Ca2+ influx and efflux across the plasma membrane in unstimulated platelets were in equilibrium at signif...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/0049-3848(89)90372-1

    authors: Fujimoto T,Fujimura K,Kuramoto A

    更新日期:1989-01-15 00:00:00

  • Risk of deep venous thrombosis (DVT) in bedridden or wheelchair-bound multiple sclerosis patients: a prospective study.

    abstract:BACKGROUND:Multiple sclerosis (MS) often causes progressive loss of mobility, leading to limb paralysis. Venous and lymphatic stasis is a risk condition for venous thromboembolism (VTE). There is, however, no data on the frequency of VTE complicating the progression of MS. The aim of this study was to assess the freque...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/j.thromres.2009.06.023

    authors: Arpaia G,Bavera PM,Caputo D,Mendozzi L,Cavarretta R,Agus GB,Milani M,Ippolito E,Cimminiello C

    更新日期:2010-04-01 00:00:00

  • Effectiveness of alternative treatments for reducing potential viral contaminants from plasma-derived products.

    abstract::An issue of great importance and continuing concern with regard to all products derived from human plasma is their safety from potential contaminants in the source material from which they are purified. Since viral contaminants are a major safety consideration with these products, a number of different methods, includ...

    journal_title:Thrombosis research

    pub_type: 杂志文章,评审

    doi:10.1016/s0049-3848(02)00044-0

    authors: Chandra S,Groener A,Feldman F

    更新日期:2002-03-01 00:00:00

  • Response to antiplatelet therapy is independent of endogenous thrombin generation potential.

    abstract:BACKGROUND:Thrombin is the most potent platelet activator, and achieves rapid platelet activation even in the presence of antiplatelet therapy. Since activated platelets respond stronger to additional stimuli, the extent of endogenous thrombin generation may in part be responsible for the reported response variability ...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/j.thromres.2013.04.008

    authors: Gremmel T,Panzer S,Steiner S,Seidinger D,Koppensteiner R,Pabinger I,Kopp CW,Ay C

    更新日期:2013-07-01 00:00:00

  • Measurement of dabigatran plasma concentrations by calibrated thrombin clotting time in comparison to LC-MS/MS in human volunteers on dialysis.

    abstract:INTRODUCTION:Dabigatran etexilate is an oral direct thrombin inhibitor. Although routine anticoagulation monitoring with dabigatran is not usually required, a simple and precise laboratory test to measure dabigatran concentrations in patient plasma may be useful in certain clinical circumstances, such as emergency situ...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/j.thromres.2014.12.021

    authors: Schmohl M,Gansser D,Moschetti V,Stangier J

    更新日期:2015-03-01 00:00:00

  • Pharmacodynamics and pharmacokinetics of C3, a heparin-derived oligosaccharide mixture, in non-human primates.

    abstract::The heparin-derived oligosaccharide C3 (C3) is currently underdevelopment for the prevention and treatment of vascular dementia and senile dementia of Alzheimer's type. C3 exhibits a molecular weight of 2200-2500 Da with a narrow distribution. The objective of the present study was to assess the pharmacodynamics and p...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/j.thromres.2003.11.008

    authors: Ma Q,Schultz C,Neville B,Jeske W,Hoppensteadt D,Cornelli U,Lee J,Lorens S,Hanin I,Fareed J

    更新日期:2003-01-01 00:00:00

  • Effect of intensive glycemic control on platelet reactivity in patients with long-standing uncontrolled diabetes.

    abstract:BACKGROUND:It has been previously shown that platelets of patients with diabetes are more reactive and less responsive to anti-platelet drugs compared with platelets from subjects without diabetes. Studies examining the effect of glycemic control on platelet reactivity have yielded conflicting data. Thus, in this study...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/j.thromres.2014.05.010

    authors: Singer J,Weissler Snir A,Leshem-Lev D,Rigler M,Kornowski R,Lev EI

    更新日期:2014-07-01 00:00:00

  • Patient-level adherence and interventions in an interdisciplinary DOAC clinic.

    abstract:BACKGROUND:Direct oral anticoagulants (DOACs) are high risk medications with short half-lives making adherence vitally important. Global measures for adherence have been described; however, there is a lack of patient-level data on adherence. METHODS:This prospective, single-center study in an interdisciplinary interna...

    journal_title:Thrombosis research

    pub_type: 信件

    doi:10.1016/j.thromres.2019.04.021

    authors: Talana AS,Huber K,Sorin M,Stalvey C,Davis K,Dietrich E

    更新日期:2019-07-01 00:00:00

  • Use of the Delphi method to facilitate antithrombotics prescription during pregnancy.

    abstract:INTRODUCTION:Management of pregnant women at risk for venous thromboembolism (VTE) remains complex. Guidelines do not definitively fix optimal strategies due to limited trial data. Our objective was to build an easy-to-use tool allowing individualised, risk-adapted prophylaxis. MATERIALS AND METHODS:A Delphi exercise ...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/j.thromres.2010.01.012

    authors: Chauleur C,Gris JC,Laporte S,Rancon F,Varlet MN,Decousus H,Mismetti P,STRATHEGE Group.

    更新日期:2010-08-01 00:00:00

  • The use of eConsults to improve access to specialty care in thrombosis medicine.

    abstract:INTRODUCTION:Electronic consultations (eConsults) are asynchronous, consultative electronic-based communications that are aimed to improve efficient and timely access to specialist advice. Our study aim was to evaluate the use and impact of the Canadian Champlain BASE™ (Building Access to Specialists through eConsultat...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/j.thromres.2017.11.002

    authors: Skeith L,Mohamed M,Karovitch A,Liddy C,Afkham A,Archibald D,Keely E

    更新日期:2017-12-01 00:00:00

  • Glycerophospholipid metabolism: back to the future.

    abstract::It has become customary to regard the various glycerophospholipids as quite similar, and the acyl groups are considered to have little influence on the behaviour of the lipids in membranes or metabolism. Nevertheless, a number of recent observations by the authors and others indicate a high degree of metabolic compart...

    journal_title:Thrombosis research

    pub_type: 杂志文章,评审

    doi:10.1016/0049-3848(92)90006-v

    authors: Holmsen H,Hindenes JO,Fukami M

    更新日期:1992-08-01 00:00:00

  • Activation contact system (ACS) and tissue factor (TF) in human amniotic fluid: measurements of ACS components and TF, and some implications on the pathophysiology of amniotic fluid embolism.

    abstract:BACKGROUND/AIM:It is believed that the amniotic fluid-derived TF, in the case of amniotic fluid embolism (AFE), contributes to acute disseminated intravascular coagulation (DIC) and obstetric shock in the mother. However, the role of amniotic fluid-derived contact phase coagulation factors that irrupt into the bloodstr...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/j.thromres.2014.10.014

    authors: Uszyński W,Żekanowska E,Uszyński M,Kieszkowski P

    更新日期:2015-04-01 00:00:00

  • Prevalence and clinical history of incidental, asymptomatic pulmonary embolism: a meta-analysis.

    abstract:CONTEXT:Recently, there has been an increasing number of reports of incidental pulmonary embolism (PE) in patients undergoing chest computer tomography (CT) for reasons other than the research of suspected PE. Natural history of incidental PE remains unclear. OBJECTIVES:To estimate the prevalence of incidental PE, to ...

    journal_title:Thrombosis research

    pub_type: 杂志文章,meta分析

    doi:10.1016/j.thromres.2010.03.016

    authors: Dentali F,Ageno W,Becattini C,Galli L,Gianni M,Riva N,Imberti D,Squizzato A,Venco A,Agnelli G

    更新日期:2010-06-01 00:00:00

  • The trimannosyl cores of N-glycans are important for the procoagulant protease-inhibitory activity of urinary protein C inhibitor.

    abstract::We investigated the relationship between the procoagulant protease-inhibitory activity and the N-glycan structures in urinary protein C inhibitor (uPCI) by sequential exoglycosidase digestions based on the N-glycan structures elucidated in this report. uPCI was glycosylated on the three potential N-glycosylation sites...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/s0049-3848(01)00342-5

    authors: Izutani W,Fujita M,Nishizawa K,Koga J

    更新日期:2001-10-01 00:00:00

  • Preparation of beta-XIIa (Hageman factor fragment) from human plasma.

    abstract::beta-Factor XIIa (beta-XIIa, Mr approximately 30,000) was isolated from human plasma by a procedure which utilized, as an initial step, the adsorption of Factor XII to celite. Activation of Factor XII and subsequent release of beta-XIIa was brought about by the proteolytic action of co-adsorbed kallikrein. Two success...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/0049-3848(82)90231-6

    authors: Tankersley DL,Alving BM,Finlayson JS

    更新日期:1982-02-15 00:00:00

  • Collagen banded fibril structure and the collagen-platelet reaction.

    abstract::Bovine hide collagen dispersions were swollen in the pH range 1.6-7.0, treated with glutaraldehyde, and dialyzed to neutral pH. The intensity with which these collagens reacted with human platelets in plasma was studied by aggregometry and scanning electron microscopy. Collagen swollen at a pH below 4.25 +/- 0.30 and ...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/0049-3848(89)90463-5

    authors: Sylvester MF,Yannas IV,Salzman EW,Forbes MJ

    更新日期:1989-07-01 00:00:00

  • Effect of aspirin-dipyridamole and heparin and their combination on venous thrombosis in hypercoagulable or thrombotic animals.

    abstract::Epsilon-amino-caproic acid (EACA) induces a clinically-useful anti-haemorrhagic, mildly thrombotic state while ellagic acid (EA) induces a severe hypercoagulable one. Reversal of these states may on occasion be necessary. The effectiveness of the antiplatelet drugs, aspirin and dipyridamole (ASA/D), in reducing thromb...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/0049-3848(96)00098-9

    authors: Iomhair MM,Lavelle SM

    更新日期:1996-06-15 00:00:00

  • The C677T polymorphism of the methylenetetrahydrofolate reductase gene is associated with the level of decrease on diastolic blood pressure in essential hypertension patients treated by angiotensin-converting enzyme inhibitor.

    abstract:OBJECTIVES:Elevated plasma homocysteine has been implicated as a risk factor for hypertension. C677T polymorphism in methylenetetrahydrofolate reductase gene (MTHFR) is a major determinant of hyperhomocysteinemia, which results in endothelial dysfunction. Angiotensin-converting enzyme (ACE) inhibitors appear to remedy ...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/j.thromres.2004.04.005

    authors: Jiang S,Hsu YH,Xu X,Xing H,Chen C,Niu T,Zhang Y,Peng S,Xu X

    更新日期:2004-01-01 00:00:00

  • Tissue Factor/ FVIIa prevents the extrinsic pathway of apoptosis by regulation of the tumor suppressor Death-Associated Protein Kinase 1 (DAPK1).

    abstract:INTRODUCTION:This study determines the impact of tissue factor (TF)-signaling on the extrinsic pathway of apoptosis in cancer cells and propose death associated protein kinase-1 (DAPK1) as a novel key regulator. MATERIALS AND METHODS:In MDA-MB-231 breast and PC3 prostate cancer cells, mRNA levels were analyzed by real...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/j.thromres.2010.11.015

    authors: Aberg M,Johnell M,Wickström M,Siegbahn A

    更新日期:2011-02-01 00:00:00

  • Effect of exercise, DDAVP, and epinephrine on the factor VIII:C/von Willebrand factor complex in normal dogs and von Willebrand factor deficient Doberman pinscher dogs.

    abstract::Endothelial cells in biopsied blood vessels from von Willebrand factor (vWf)-deficient Doberman pinscher dogs contain immunologically detectable vWf. These dogs and normal dogs were treated with DDAVP (0.6 microgram/kg) and epinephrine (0.5 microgram/kg/min for 30 minutes) and were exercised, using 5 different exercis...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/0049-3848(90)90198-l

    authors: Meyers KM,Wardrop KJ,Dodds WJ,Brassard J

    更新日期:1990-01-01 00:00:00

  • Platelet dysfunction in vascular pathologies and how can it be treated.

    abstract::Cardiovascular diseases are one of the leading causes of morbidity and mortality in industrialized countries, and although many processes play a role in the development of vascular disease, thrombosis is the primary event that precipitates stroke and acute coronary syndromes. The blood platelets are of significant imp...

    journal_title:Thrombosis research

    pub_type: 杂志文章,评审

    doi:10.1016/j.thromres.2011.09.026

    authors: Alexandru N,Popov D,Georgescu A

    更新日期:2012-02-01 00:00:00

  • Erik von Willebrand.

    abstract::In 1926, Erik von Willebrand, a Finnish physician, published the first manuscript describing a haemorrhagic disorder in people who were living on the Aland islands off the coast of Finland. This disorder is now known by the name of its discoverer. Since this early observation, von Willebrand disease has been extensive...

    journal_title:Thrombosis research

    pub_type: 传,历史文章,杂志文章

    doi:10.1016/j.thromres.2007.03.010

    authors: Berntorp E

    更新日期:2007-01-01 00:00:00

  • Acute carotid artery occlusive thrombosis and its pharmacological prevention in the rabbit.

    abstract::A simple and reproducible method to induce an occlusive thrombus in rabbit carotid artery is reported. Rabbits were anesthetized and prepared to record arterial pressure, heart rate, and carotid blood flow. A critical stenosis of a damaged carotid artery was obtained using an external plastic cylinder. Complete occlus...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/0049-3848(91)90147-o

    authors: Bevilacqua C,Finesso M,Prosdocimi M

    更新日期:1991-05-15 00:00:00