Abstract:
BACKGROUND:Many heparin-induced thrombocytopenia (HIT) antibodies cause platelet activation in the serotonin release assay (SRA) in the absence of heparin. This in vitro observation may help unravel the mechanism of delayed-onset HIT, where seropositive patients develop thrombocytopenia and associated thrombosis after cessation of heparin. OBJECTIVE:Studies were conducted to examine the relationship between platelet environment, surface PF4 expression, and the extent of heparin-independent platelet activation in the SRA. METHODS:Ex vivo platelets were washed and labeled for SRA, then used either before or after 45 minutes of recovery at 37 degrees C. HIT antibody-mediated serotonin release in the absence of heparin was compared to the extent of surface staining of the platelets with fluorescent anti-human PF4 antibodies. RESULTS:Handling of platelets for in vitro studies resulted in transient expression of surface PF4, and it was during this interval that platelets were most sensitive to activation by HIT antibodies in the absence of heparin. Heparin-independent platelet activation was attenuated when SRA-positive specimens were retested after platelets were incubated 45 minutes at 37 degrees C. Surface PF4 expression was diminished on the rested platelets, compared to the same platelets labeled immediately after handling. Thus compared to rested platelets, mildly activated platelets had elevated surface PF4 expression and a higher level of HIT antibody-mediated, heparin-independent platelet activation. CONCLUSION:Surface expression of PF4 reflects HIT antigen presentation, and varies with the physiological state of platelets. Thus there can be differences in HIT antibody target availability among patients which may explain the variability in consequences of HIT antibody seropositivity.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Prechel MM,Jeske WP,Walenga JMdoi
10.1016/j.thromres.2009.10.021subject
Has Abstractpub_date
2010-04-01 00:00:00pages
e143-8issue
4eissn
0049-3848issn
1879-2472pii
S0049-3848(09)00499-Xjournal_volume
125pub_type
杂志文章abstract::Prothrombin time is a standard screening test to monitor patients treated with oral anticoagulants. We hypothesize that INR measurements will not vary a lot within a given day in a given patient. The present study was thus conducted to compare the change of the international normalized ratio (INR) of prothrombin time ...
journal_title:Thrombosis research
pub_type: 临床试验,杂志文章
doi:10.1016/s0049-3848(02)00339-0
更新日期:2002-09-01 00:00:00
abstract::The hemolytic uremic syndrome involves the presence of Shiga toxin producing strains of Escherichia coli and is associated with thrombocytopenia, platelet activation, and microthrombi formation. We have, therefore, investigated the ability of Shiga toxin isotypes 1 and 2 to cause or enhance platelet aggregation under ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(00)00191-2
更新日期:2000-06-01 00:00:00
abstract:INTRODUCTION:The contact system proteins factor XII (FXII), prekallikrein (PK) and high molecular weight kininogen (HK) have roles in coagulation, fibrinolysis, thrombin-induced platelet activation, cell adhesion and angeogenisis. It has been suggested that inherited or acquired deficiencies of these proteins may be ri...
journal_title:Thrombosis research
pub_type: 临床试验,杂志文章
doi:10.1016/j.thromres.2004.05.005
更新日期:2004-01-01 00:00:00
abstract::We report an inbred family with two cases of homozygous protein C deficiency and review 11 other such cases. Both patients presented in the second half of their first year of life with recurrent rapidly disappearing ecchymotic skin lesions, disseminated intravascular coagulation, and venous thrombosis. Successful trea...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/0049-3848(89)90202-8
更新日期:1989-03-01 00:00:00
abstract:INTRODUCTION:Flow cytometry (FCM) is the most commonly used method for detection of platelet-derived microparticles (PDMPs), but it is poorly standardized and mainly used for "bedside" analyses in fresh samples. If PDMPs could be analyzed in previously frozen samples it would increase the usefulness of the method. Howe...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2009.10.006
更新日期:2010-03-01 00:00:00
abstract:INTRODUCTION:Portal vein thrombosis (PVT) is a well-known complication in patients with liver cirrhosis (LC). The aim of this study is to investigate the outcomes of cirrhotic patients with PVT treated with low-molecular-weight heparin (LMWH). METHOD:Ninety-one LC patients with PVT were treated with dalteparin or enox...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2018.01.032
更新日期:2018-03-01 00:00:00
abstract:AIM:Postprandial platelet activation was related to postprandial insulin rather than glucose levels in a previous meal insulin study in type 2 diabetes mellitus (T2DM). We therefore compared postprandial platelet activation in type 1 (T1DM) patients without insulin secretion and T2DM patients with high postprandial ins...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2016.03.009
更新日期:2016-05-01 00:00:00
abstract::Experience with tranexamic acid, an indirect fibrinolytic inhibitor, started as soon as it was released from Shosuke Okamoto's lab in the early 1960s. It was first prescribed to females with heavy menstrual blood loss and to patients with hereditary bleeding disorders. Soon the indications were widened to elective sur...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/j.thromres.2014.11.012
更新日期:2015-02-01 00:00:00
abstract::The fibrinolytic system was evaluated in a patient with homozygous protein C deficiency as well as in several members of his family with a partial deficiency of this protein. Before anticoagulant therapy the patient showed skin lesions which quickly disappeared after administration of fresh plasma. After anticoagulant...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(86)90260-4
更新日期:1986-05-01 00:00:00
abstract::In this study, we investigated the effects of a 3-min insulin incubation both at physiological and at supraphysiological concentrations on platelet aggregation and intraplatelet cyclic guanosine monophosphate (cGMP) levels both in the absence and in the presence of phosphodiesterase inhibition. We observed that insuli...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(96)00051-5
更新日期:1996-04-01 00:00:00
abstract:INTRODUCTION:Although the procoagulant reactivity of monocytes largely depends on expression and cell surface presentation of tissue factor (TF), little is known about the impact of tissue factor pathway inhibitor (TFPI) on regulation of TF function on the monocyte surface. MATERIALS AND METHODS:Peripheral blood monon...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2010.07.014
更新日期:2010-11-01 00:00:00
abstract::Individuals with more than one defect in the natural anticoagulant system exhibit an increased risk for thrombosis. We report on a family with two cases of combined protein C (PROC) and protein S (PROS) deficiency, five cases of isolated PROC deficiency Type I, and two cases of isolated PROS deficiency Type I. PROC an...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(01)00232-8
更新日期:2001-07-01 00:00:00
abstract:BACKGROUND:Clinical and epidemiological studies have associated selective COX-2 inhibitors with an increased risk of cardiovascular events. There are no clinical studies on the possible effects of these drugs on secondary hemostasis. The hypothesis for this study is that the use of selective COX-2 inhibitors could affe...
journal_title:Thrombosis research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/j.thromres.2009.01.017
更新日期:2009-06-01 00:00:00
abstract:BACKGROUND:Histamine is classified as an inflammatory mediator and has been reported to have anti- as well as pro-inflammatory properties. The aim of this study was to explore the role of histamine on the production of LPS-induced tissue factor (TF) activity and TNFα in monocytes of whole blood in the absence and prese...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2013.12.022
更新日期:2014-03-01 00:00:00
abstract:INTRODUCTION:The TF-FVIIa complex is the primary activator of coagulation. Elevated levels of microvesicle (MV) bearing tissue factor (TF)-dependent procoagulant activity are detectable in patients with an increased risk of thrombosis. Several methods have been described to measure MV TF activity but they are hampered ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2019.07.011
更新日期:2019-10-01 00:00:00
abstract:INTRODUCTION:Hemodialysis grafts often fail because of stenosis at the venous anastomosis resulting in thrombosis. Percutaneous intervention involves thrombolysis with plasminogen activators, mechanical removal of thrombus, and angioplasty of the stenotic lesions. OBJECTIVES:This article describes a phase I trial usin...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2008.06.020
更新日期:2008-01-01 00:00:00
abstract::Microheterogeneity of antithrombin III (ATIII) was examined in pooled normal human plasma and serum by IEF/PA and immunoblotting techniques. A pattern consisting of 8 bands varying in isoelectric point from 4.7 to 5.2 was observed in citrated plasma following IEF in a pH range of 4-6. A similar pattern was observed in...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(85)90331-7
更新日期:1985-10-15 00:00:00
abstract::Enzymatic treatment used for passaging of endothelial cells may induce release of von Willebrand factor (vWF). Decreased ability to replenish intracellular stores results in decreased secretion of vWF in later passages of cells. Since both trypsin and pancreatin complex have been used for passaging endothelial cells, ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(00)00401-1
更新日期:2001-02-15 00:00:00
abstract::Central venous catheter-related thrombosis (CRT) is a complication seen in patients requiring long-term intravenous access. Treatment of CRT is not standardized and international guidelines for treatment are based on extrapolation of evidence from lower extremity thrombosis. We performed a systematic review of the lit...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/j.thromres.2015.08.020
更新日期:2015-12-01 00:00:00
abstract:INTRODUCTION:Prediction of venous thromboembolism (VTE) occurrence in cancer patients using individual risk factors may contribute to preventing the burden of disease associated with VTE. Congestive heart failure in patients with cancer may increase the risk of VTE and worsen the prognosis. AIM:We sought to investigat...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/S0049-3848(16)30134-7
更新日期:2016-04-01 00:00:00
abstract::Defibrotide both "in vitro" and "ex vivo" decreases PMN adhesion to nylon columns. This activity is blunted by 8-phenyltheophylline, a known blocker of adenosine A1/A2 receptors, suggesting that Defibrotide acts on that kind of receptors. Indomethacin, a blocker of PGI2 synthesis, did not cancel DFT's activity, sugges...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(95)00178-t
更新日期:1995-11-15 00:00:00
abstract::There is growing evidence on the superior efficacy and safety of low molecular weight heparins (LMWHs) over unfractionated heparin (UFH) for the treatment of both venous and arterial thromboembolism. Heparin exerts its function by potentiating antithrombin and by mobilizing tissue factor pathway inhibitor (TFPI) into ...
journal_title:Thrombosis research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0049-3848(98)00079-6
更新日期:1998-08-15 00:00:00
abstract:INTRODUCTION:Several hemostatic system components, including factor X (FX), contribute to cancer progression. The Protein Z (PZ)/protein Z-dependent protease inhibitor (ZPI) complex directly inhibits factor Xa proteolytic activity. The aim of this study was to determine the antigenic distribution of ZPI and PZ, in rela...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2011.10.027
更新日期:2012-04-01 00:00:00
abstract::Danazol, a synthetic androgen reported to increase factor VIII and IX activity levels, was given to 6 hemophiliacs. With danazol therapy (600 mg/da) the APTTs shortened by 30-45% of pre-treatment times. However, the activity levels of the deficient factors did not increase significantly nor consistently with the APTT ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(85)90126-4
更新日期:1985-07-01 00:00:00
abstract::The antithrombotic actions of selective factor Xa (FXa) inhibitors, recombinant tick anticoagulant peptide (rTAP) and DX-9065a, were evaluated in experimental thrombosis models in anesthetized rats. In the first model, thrombosis was induced by exposing flowing blood to a silk thread anchored in an arteriovenous (AV) ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(96)00112-0
更新日期:1996-07-15 00:00:00
abstract:INTRODUCTION:The effects of multiple inherited and acquired thrombophilic defects on the outcome of in-vitro fertilization (IVF) remain unexplored. The aim of this study was to evaluate the association between multiple thrombophilia and clinical outcomes in a large prospective cohort of women undergoing IVF. MATERIALS...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2018.05.006
更新日期:2018-07-01 00:00:00
abstract::Tissue factor (TF) is an integral membrane glycoprotein that serves as a cofactor for blood coagulation factor VIIa. The induction of TF on the surface of endothelial cells is initiated by various kinds of stimuli including lipopolysaccharide (LPS), interleukin-1 beta (IL-1 beta), and tumor necrosis factor alpha (TNF ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(94)90002-7
更新日期:1994-04-15 00:00:00
abstract::Thrombus formation was electrically induced in the left common carotid artery of rats with preoccluded both vertebral and right carotid arteries. Following thrombus induction, animals became unresponsive and lost their righting reflex. The loss of righting reflex was observed when cortical tissular pO2 reached 55 +/- ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(87)90340-9
更新日期:1987-10-01 00:00:00
abstract::Owing to the beneficial health effects on human cardiovascular system, soybeans and soy-related products have been a focus of intensive research. Soy isoflavones are known to be primarily responsible for the soy-related biological effects including anti-platelet activity but its in vivo relevancy has not been fully ve...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2013.01.002
更新日期:2013-03-01 00:00:00
abstract::High molecular weight kininogen has been shown to be the principal plasma inhibitor of cellular thiol proteases including cathepsins B, H and L and calpains 1 and 2. Since these same enzymes have been reported to be elevated in animals with muscular dystrophy, we studied plasmas from hamsters with muscular dystrophy a...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(89)90041-8
更新日期:1989-04-15 00:00:00