Abstract：
INTRODUCTION:The TF-FVIIa complex is the primary activator of coagulation. Elevated levels of microvesicle (MV) bearing tissue factor (TF)-dependent procoagulant activity are detectable in patients with an increased risk of thrombosis. Several methods have been described to measure MV TF activity but they are hampered by limited sensitivity and specificity. The aim of this work was to increase the sensitivity of the MV TF activity assay (called Chapel Hill assay). MATERIAL AND METHODS:Improvements of the MV TF activity assay included i/ speed and time of centrifugation, ii/ use of a more potent inhibitory anti-TF antibody iii/ use of FVII and a fluorogenic substrate to increase specificity. RESULTS:The specificity of the MV TF activity assay was demonstrated by the absence of activity on MV derived from a knock-out-TF cell line using an anti-human TF monoclonal antibody called SBTF-1, which shows a higher TF inhibitory effect than the anti-human TF monoclonal antibody called HTF-1. Experiments using blood from healthy individuals, stimulated or not by LPS, or plasma spiked with 3 different levels of MV, demonstrated that the new assay was more sensitive and this allowed detection of MV TF activity in platelet free plasma (PFP) samples from healthy individuals. However, the assay was limited by an inter-assay variability, mainly due to the centrifugation step. CONCLUSIONS:We have improved the sensitivity of the MV TF activity assay without losing specificity. This new assay could be used to evaluate levels of TF-positive MV as a potential biomarker of thrombotic risk in patients.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Vallier L,Bouriche T,Bonifay A,Judicone C,Bez J,Franco C,Guervilly C,Hisada Y,Mackman N,Houston R,Poncelet P,Dignat-George F,Lacroix Rdoi
10.1016/j.thromres.2019.07.011subject
Has Abstractpub_date
2019-10-01 00:00:00pages
64-74eissn
0049-3848issn
1879-2472pii
S0049-3848(19)30294-4journal_volume
182pub_type
杂志文章abstract：:Initiation of clotting cascade by tissue factor (TF): Factor VII (FVII) complex is essential for hemostasis however pathologic expression of TF leads to thrombosis. In contrast, the contact pathway factor XII, and to the smaller extent FXI are dispensable for normal hemostasis but still contribute to pathologic thromb...
journal_title：Thrombosis research
pub_type：
doi：10.1016/j.thromres.2014.03.001
更新日期：2014-05-01 00:00:00
abstract：:Severe rejection crises occurred in 13 out of 20 cadaver kidney recipients; removal of the graft was inevitable in 10 cases. The plasminogen levels were found to be lower (76.5 +/- 2.9%) in the 13 patients with impaired graft acceptance (group B) than in the 7 patients experiencing only moderate rejection (group A, 93...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(86)90257-4
更新日期：1986-05-01 00:00:00
abstract：:Since the first description by Dahlbäck et al (1) of a hereditary defect in the plasma of three unrelated thrombophilic patients characterized by a poor anticoagulant response to activated protein C (APC), many reports have shown a high prevalence of the APC resistance in both the general and thrombophilic populations...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(97)00022-4
更新日期：1997-02-15 00:00:00
abstract：:Heat treatment is employed to diminish the transmission of hepatitis when blood products are administered. It is possible that such a procedure could reduce the biological activity of the proteins and induce changes in structure and aggregation state. We have therefore made in vitro and pharmacokinetic studies of heat...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(87)90435-x
更新日期：1987-12-15 00:00:00
abstract：:One of the major pathogens of periodontitis, Porphyromonas gingivalis (P. gingivalis), has the ability to aggregate human platelets. To investigate the interaction between P. gingivalis and human platelets in platelet rich plasma (PRP), platelet aggregation was measured by an aggregometer based on laser light scatteri...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2008.03.011
更新日期：2008-01-01 00:00:00
abstract：:SK, t-PA or APSAC were incubated in human plasma (adjusted to 300,000 platelets/mm3), in vitro, for up to 90 minutes using concentrations which were equivalent to those achieved in the treatment of AMI patients. Aggregation was measured in response to ADP and collagen. SK inhibited platelet aggregation after a 60 minu...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(90)90105-l
更新日期：1990-11-15 00:00:00
abstract：:Dextran sulfate and polybrene were immobilized on polypropylene tubes with plastic adhesive. Serum albumin was also immobilized on the tube by heat-denaturation. In the those tubes thrombin was protected from inactivation in the presence of 0.1 M NaCl remarkably. Cholesterol coated on the tube also protected the enzym...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(88)90277-0
更新日期：1988-07-01 00:00:00
abstract：INTRODUCTION:Guidelines recommend the assessment of stroke and bleeding risk before initiating warfarin anticoagulation in patients with atrial fibrillation. Many of the elements used to predict stroke also overlap with bleeding risk in atrial fibrillation patients and it is tempting to use stroke risk scores to effici...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2014.05.034
更新日期：2014-08-01 00:00:00
abstract：INTRODUCTION:The kinetics of the thrombin inhibition by heparin cofactor II (HCII) and antithrombin (AT) have been studied as a function of the concentration of a dermatan sulfate (DS) from the skin of the ray Raja radula. MATERIALS AND METHODS:The initial concentrations of inhibitor (I), HCII or AT, and thrombin (E) ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2008.10.003
更新日期：2009-04-01 00:00:00
abstract：INTRODUCTION:Though thrombin-activatable fibrinolysis inhibitor (TAFI) may contribute to hypercoagulability during pregnancy, limited data are available on the role of TAFI in women with recurrent fetal loss. MATERIAL/METHODS:We performed a case-control study aimed at evaluating any possible association between TAFI l...
journal_title：Thrombosis research
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.thromres.2011.09.029
更新日期：2012-08-01 00:00:00
abstract：:The placental transfer of Org 10172, a low-molecular weight heparinoid, was determined in 12 awake late-pregnant guinea pigs. Nine animals receiving placebo served as controls. After 5 days i.v. treatment with Org 10172 (2 x 300 anti-Xa U/kg/day), anti-Xa activity in fetal plasma amounted to 2.4% of the maternal conce...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(86)90002-2
更新日期：1986-11-01 00:00:00
abstract：INTRODUCTION:Preterm infants often require assisted ventilation, however ventilation when applied to the immature lung can initiate ventilator-induced lung injury (VILI). The biotrauma which underscores VILI is largely undefined, and is likely to involve vascular injury responses, including hemostasis. We aimed to use ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2020.09.036
更新日期：2020-12-01 00:00:00
abstract：BACKGROUND:Intravenous fluids can impair coagulation and affect the endothelial glycocalyx, whereas glycocalyx shedding itself can cause an impairment of clot formation and firmness. We hypothesized that hydroxyethyl starch 6% (130/0.4) has a more distinct effect on coagulation and glycocalyx shedding than albumin 5%. ...
journal_title：Thrombosis research
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.thromres.2019.10.020
更新日期：2019-11-01 00:00:00
abstract：:G619, a 4-OH-isophthalic acid derivative, was studied for its capacity to inhibit platelet aggregation. G619 dose-dependently inhibited U46619, collagen, ADP, PAF, thrombin and epinephrine-induced platelet aggregation in vitro. The IC50 values for inhibition of U46619-induced human and rabbit platelet aggregation were...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(92)90283-g
更新日期：1992-05-15 00:00:00
abstract：:Envenomation caused by Lonomia obliqua caterpillars is an increasing problem in Southern Brazil. The clinical profile is characterized by a profound hemorrhagic disorder. In the present study, we describe the characterization of a fibrin(ogen)olytic factor (lonofibrase) isolated from a venomous secretion of the caterp...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2004.01.012
更新日期：2004-01-01 00:00:00
abstract：:An in vivo rabbit model of venous thrombosis which includes physiological blood flow was used to compare the efficacy of the potent and specific factor Xa inhibitor recombinant tick anticoagulant peptide (rTAP) with standard heparin in the prevention of venous thrombus formation. In anesthetized rabbits, an autologous...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(93)90200-8
更新日期：1993-08-15 00:00:00
abstract：BACKGROUND:In patients who are receiving warfarin therapy and require cataract surgery, it may be possible to continue warfarin in the perioperative period but the safety of this management strategy has not been systematically evaluated. METHODS:We performed a systematic review of the literature to assess the safety (...
journal_title：Thrombosis research
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.thromres.2009.01.007
更新日期：2009-07-01 00:00:00
abstract：:An experimental in vitro model of the hemodynamics that occur in atrial fibrillation (AFib) in the left atrial appendage (LAA) was developed to study changes in human endothelial cell thrombotic potential. We applied human-derived sinus rhythm and AFib hemodynamic shear stress patterns to primary human endothelial cel...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2016.04.022
更新日期：2016-07-01 00:00:00
abstract：INTRODUCTION:Randomized clinical trials have demonstrated non-inferiority of rivaroxaban compared with vitamin K antagonists (VKAs) in the treatment of venous thromboembolism (VTE). Our objective was to analyze in real life, tolerance, recurrence, bleeding and adverse events of rivaroxaban in patients with acute sympto...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2014.06.032
更新日期：2014-09-01 00:00:00
abstract：OBJECTIVE:The diallelic glycoprotein IIIa polymorphism P1A1/A2 was attributed to be an inherited risk factor for coronary events. Whether this polymorphism affects response to aspirin in patients with coronary artery disease is not known. METHODS:We assessed thrombin generation (prothrombin fragment F1+2) in consecuti...
journal_title：Thrombosis research
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.thromres.2006.03.005
更新日期：2007-01-01 00:00:00
abstract：:The presence of Factor XIII subunit a was demonstrated in human monocytes by immunoperoxidase staining using specific antisera against Factor XIII and its subunits. This finding was verified by immunobiochemical techniques, as well. In an immunoblotting system after SDS polyacrylamide gel electrophoresis of denatured ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(85)90069-6
更新日期：1985-02-01 00:00:00
abstract：:Lupus anticoagulants (LA) are IgG or IgM antibodies which prolong phospholipid-dependent coagulation tests. For the detection and quantitation of such antibodies, we have developed an ELISA with cephalin as the coating antigen. The sensitivity of this assay was compared to the activated partial thromboplastin time (AP...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(90)90323-5
更新日期：1990-01-15 00:00:00
abstract：:In this study we examined in 100 patients testing positive for Helicobacter pylori infection whether successful eradication therapy with pantoprazole, clarithromycin, and metronidazole alters fibrinogen and other acute phase response markers. Of 100 patients, only 11 showed a fibrinogen level above 300 mg/dL. Successf...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(99)00230-3
更新日期：2000-03-15 00:00:00
abstract：:Dietary intake of fish oils, rich in the polyunsaturated fatty acids (PUFAs) docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), has given inconsistent results as to their influence on the plasma fibrinogen level (1, 2, 3, 4, 5, 6). In the present study we have examined the effects of various fatty acids, the ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(95)00152-h
更新日期：1995-10-01 00:00:00
abstract：INTRODUCTION:This study aimed to determine the risk factors for subclinical umbilical catheter-related thrombosis (UCRT) and its association with clinical morbidities. MATERIALS AND METHODS:In infants without any symptoms associated with umbilical catheterization, the presence of thrombosis was monitored using abdomin...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2020.05.034
更新日期：2020-10-01 00:00:00
abstract：:Whole blood electrical aggregometry (WBEA) has become an accepted method to gain quick information on platelet disorders. Compared to the optical method WBEA is closer to physiology and less complicated, but on the other hand more difficult to standardize. Different approaches have been attempted in the past to improv...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(90)90232-2
更新日期：1990-12-15 00:00:00
abstract：:Algal fucoidans possess a wide variety of biological activities, including anticoagulation and antithrombosis, making them potential candidates for clinical use. We assessed the antiaggregant, anticoagulant and antithrombotic activities and the underlying mechanism of the low- and high-molecular weight fucoidans from ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2010.02.011
更新日期：2010-05-01 00:00:00
abstract：:A semi-synthetic 1-0-alkyl-2-acetyl-sn-glycero-3-phosphocholine (alkylacetyl-GPC) was shown to be highly species selective in its capacity to cause platelet aggregation and serotonin release. No effects were elicited on the rat or mouse platelets while platelets from human, dog, cat, rabbit, guinea pig and horse were ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(82)90234-1
更新日期：1982-02-15 00:00:00
abstract：:We compared the effects of dipyridamole, RA-642, and mopidamol on platelet activity and thromboxane/prostacyclin balance in relation to the degree of retinal vascularization in a model of experimental streptozotocin-induced diabetes in rats. After 3 months, collagen-induced platelet aggregation in whole blood was 25% ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(96)00004-7
更新日期：1996-02-01 00:00:00
abstract：:Recombinant factor IX (rFIX) is increasingly used to treat patients with hemophilia B. CHO (Chinese Hamster Ovary) cells are commonly used for the production of rFIX but they have a limited capacity for introducing post-translational modifications (PTM) leading to incomplete γ-carboxylation, low phosphorylation and su...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2012.08.313
更新日期：2012-11-01 00:00:00
