Abstract：
:Enzymatic treatment used for passaging of endothelial cells may induce release of von Willebrand factor (vWF). Decreased ability to replenish intracellular stores results in decreased secretion of vWF in later passages of cells. Since both trypsin and pancreatin complex have been used for passaging endothelial cells, we analyzed the effects of successive passaging with these two enzyme preparations on the storage and secretion of vWF by human umbilical vein endothelial cells (HUVECs). Measurements were performed after the second to fifth passages. Cytoplasmic vWF was analyzed by indirect immunofluorescence and secreted vWF was measured in the supernatant of cultured HUVECs by ELISA. In trypsin-passaged cells, secreted vWF decreased progressively from passages 2 to 5. Respective concentrations were 355.0 +/- 30.4, 201.0 +/- 84.5, 150.0 +/- 1.4 and 120.5 +/- 38.9 ng vWF/10(5) cells. Comparatively, pancreatin-passaged cells secreted even less vWF protein (P = .001) at passages 4 and 5 (108.5 +/- 12.0 and 100.0 +/- 4.2 ng/10(5) cells, respectively) and had less vWF-positive cytoplasmic granules per cell. Thus, in experiments involving measurements of endothelial vWF, the use of low passage cells is recommendable and passaging with a pure trypsin preparation appears to be more appropriate.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Lopes AA,Peranovich TM,Maeda NY,Bydlowski SPdoi
10.1016/s0049-3848(00)00401-1subject
Has Abstractpub_date
2001-02-15 00:00:00pages
291-7issue
4eissn
0049-3848issn
1879-2472pii
S0049-3848(00)00401-1journal_volume
101pub_type
杂志文章abstract：:A new approach to the investigation of accumulation of acridine derivatives and release by platelets is proposed. At high concentrations of acridine orange (AO) inside dense bodies, a two component fluorescence spectrum with maxima at 530 and 640 nm has been recorded. Computer deconvolution of spectra provided additio...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(87)90102-2
更新日期：1987-09-15 00:00:00
abstract：:Cilostazol is a newly developed antiplatelet drug that has been widely applied for clinical use. Its antiplatelet action appears to be mainly related to inhibition of intracellular phosphodiesterase activity. Our study was designed to investigate inhibitory effects of cilostazol on the expression of activation-depende...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(98)00172-8
更新日期：1999-02-01 00:00:00
abstract：BACKGROUND:The Clauss fibrinogen method and thrombin clotting time (TCT) are still routinely used in patients with cirrhosis to define fibrinogen concentration and clotting potential. The thromboelastometric functional fibrinogen FIBTEM assay evaluates the strength of fibrin-based clots in whole blood, providing inform...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2015.04.003
更新日期：2015-06-01 00:00:00
abstract：:Owing to the beneficial health effects on human cardiovascular system, soybeans and soy-related products have been a focus of intensive research. Soy isoflavones are known to be primarily responsible for the soy-related biological effects including anti-platelet activity but its in vivo relevancy has not been fully ve...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2013.01.002
更新日期：2013-03-01 00:00:00
abstract：:The heparin-binding properties of human plasma apolipoproteins B-100 and E (apoB-100 and E) of low density lipoproteins (LDL), thrombin, and antithrombin-III (AT-III) were investigated. A highly reactive heparin (HRH) to apoB-100 was isolated by chromatography of crude heparin on a column of LDL immobilized to Affi-Ge...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(84)90258-5
更新日期：1984-06-15 00:00:00
abstract：:Severe bleeding took place in a patient when abdominal aneurysm was removed by operation. Bleeding continued after infusion of heparin, antithrombin III (ATIII), fresh platelets and fresh blood. Infusion of tranexamic acid resulted in an immediate cessation of bleeding and improvement of his general condition. Sometim...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(90)90167-b
更新日期：1990-04-15 00:00:00
abstract：INTRODUCTION:A close association exists between oral health and cardiovascular disease. Periodontal disease induces early vascular changes while oral pathogens have been detected in sub gingival and atheromatous plaques. We examined the interrelationship between Periodontal disease, oral bacteria, surrogate sub-clinica...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2013.04.023
更新日期：2013-07-01 00:00:00
abstract：:The placenta is the highly specialised organ of pregnancy that supports the normal growth and development of the fetus. Growth and function of the placenta are precisely regulated and coordinated to ensure the exchange of nutrients and waste products between the maternal and fetal circulatory systems operates at maxim...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2004.06.038
更新日期：2004-01-01 00:00:00
abstract：:The effect of alpha-tocopherol on various endothelial cell functions was evaluated in vitro using cultures of human umbilical vein endothelial cells. Prostacyclin synthesis, plasminogen activator activity and von Willebrand factor activity were evaluated in control and alpha-tocopherol supplemented cultures. Alpha-toc...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(88)90203-4
更新日期：1988-05-15 00:00:00
abstract：BACKGROUND:Right heart dysfunction is a crucial factor in risk stratification of normotensive patients with pulmonary embolism. Apart from biomarkers, determinants of right heart dysfunction in this group of patients are not yet well established. AIM AND METHOD:In order to identify such determinants, we analysed data ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2010.06.009
更新日期：2010-09-01 00:00:00
abstract：:The role of free radical generation and its scavenging enzymes in circulating mice polymorphonuclear leukocytes (PMNLs) has been studied following pulmonary thromboembolism. Levels of malonaldehyde (MDA), 02- radical generation, activity of superoxide dismutase (SOD), catalase (CAT), lactate dehydrogenase (LDH), myelo...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(92)90183-b
更新日期：1992-05-01 00:00:00
abstract：:Human fibrinogen exposed to 46.5 degrees C was subjected to gel permeation chromatography. The protein eluted in two distinct peaks. The first peak appeared in the void volume containing soluble fibrinogen aggregates, while the other peak represented monomeric fibrinogen. In contrast to the monomeric peak material, th...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(96)00063-1
更新日期：1996-04-15 00:00:00
abstract：BACKGROUND:In the rat liver, growth hormone (GH) affects the synthesis of vitamin-K-dependent factors, including Protein C (prot.C) and protein S (prot.S), two natural anticoagulants that prevent hypercoagulable states. Adults with GH deficiency (GHD) are at risk of thrombotic events. High circulating levels of PAI-1 a...
journal_title：Thrombosis research
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1016/j.thromres.2010.08.028
更新日期：2010-12-01 00:00:00
abstract：INTRODUCTION:The low-molecular-weight heparins have been demonstrated to have antiangiogenic effects in various assays. We aimed to demonstrate and compare the antiangiogenic effects of four types of commercially available low-molecular weight heparins in the chick embryo chorioallantoic membrane model. MATERIALS AND ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2011.05.005
更新日期：2011-10-01 00:00:00
abstract：BACKGROUND:Early aspirin treatment is widely used to inhibit platelet activity and to reduce morbidity and mortality in patients presenting with an acute myocardial infarction or a stroke. A number of different aspirin formulations have been used for this purpose; however, a comparison of their effectiveness in inhibit...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2005.10.001
更新日期：2006-01-01 00:00:00
abstract：:Intra-ventricular hemorrhage (IVH) occurs predominantly in very low birth weight premature infants. Survivors of severe IVH frequently experience long-term consequences including major neurological deficits. Advances in neonatal and obstetric care in the last decades, have led to a steady decline in mortality and in t...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/S0049-3848(11)70032-9
更新日期：2011-02-01 00:00:00
abstract：INTRODUCTION:The prevalence and etiology of occult bleeding among patients on warfarin who are screened systematically for new anemia is largely unknown. We aimed to estimate the usefulness of following hemoglobin and mean red cell volume (MCV) with INR in order to screen for developing anemia as an indicator of occult...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2012.05.005
更新日期：2012-09-01 00:00:00
abstract：INTRODUCTION:Rivaroxaban is an oral, selective direct factor Xa inhibitor approved for several indications in patients at risk of thrombotic events. One limitation of its clinical use is the lack of data pertaining to its reversal in situations where urgent response is critical (e.g. acute bleeding events or emergency ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2015.01.007
更新日期：2015-03-01 00:00:00
abstract：:Von Willebrand disease (VWD) is classified into three primary subtypes; type 1 includes partial quantitative deficiency, type 2 (A, B, M and N) includes qualitative defects and type 3 includes virtually complete deficiency of von Willebrand factor (VWF). Type 2A includes variants with decreased platelet adhesion cause...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2007.03.014
更新日期：2007-01-01 00:00:00
abstract：:Defibrotide (D) was labelled with 125I or with 32P. The radiolabelled compounds ([125I]-Defibrotide ([125I]-D), [32P]-Defibrotide ([32P]-D) retained the same profibrinolytic activity, in vitro, as the parent drug, suggesting that the labelling procedures had not modified the pharmacological properties of D and hence t...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(95)00213-8
更新日期：1996-01-01 00:00:00
abstract：:While the role of platelets in hemostasis is well characterized from a biological perspective, the biophysical interactions between platelets and their mechanical microenvironment are relatively unstudied. The field of cellular mechanics has developed a number of approaches to study the effects of extracellular matrix...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2013.12.037
更新日期：2014-04-01 00:00:00
abstract：:In adult patients with sickle cell disease two distinct subphenotypes have previously been defined: patients with the viscosity-vaso-occlusion subphenotype (VVO) suffer mainly from vaso-occlusive pain crises and have a relatively high hemoglobin concentration. Patients classified as the hemolysis-endothelial dysfuncti...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2013.10.006
更新日期：2013-01-01 00:00:00
abstract：:The extrinsic fibrinolytic system was assessed among 124 consecutive survivors of acute myocardial infarction below 70 years of age. In samples drawn 3 months after discharge from hospital, the PAI-1 levels were higher and the tPA activities were lower than among elderly healthy controls. In contrast, the AMI survivor...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(87)90428-2
更新日期：1987-12-15 00:00:00
abstract：INTRODUCTION:Synovitis is an early set and persistent change during the progression of osteoarthritis, which causes symptoms such as pain and swelling of the joints. Platelet-rich plasma (PRP) can release quantities of growth factors and cytokines, and is proved effected in promoting restoration of multiple soft tissue...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2019.11.017
更新日期：2020-02-01 00:00:00
abstract：:The clinical feature in patients with congenital factor VII deficiency is in part dependent on the underlying genetic defect, but the mechanisms influencing the genotype-phenotype correlation remain to be fully elucidated. In addition, thromboembolic events have been reported. Compensatory mechanisms involving vitamin...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(00)00410-2
更新日期：2001-03-15 00:00:00
abstract：INTRODUCTION:Plasma viscosity (PV) constitutes an independent important predictor of initial and recurrent cardiac events and mortality. It has been suggested that there is a geographical variation in PV values related to coronary event rates. Little information exists regarding PV in Spain. Therefore, our objective wa...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2006.11.009
更新日期：2007-01-01 00:00:00
abstract：INTRODUCTION:After major liver surgery, there are risks of both postoperative bleeding and thrombosis. Routine coagulation monitoring is indicated, but may not provide adequate clinical guidance. Thus, we described the clotting status in a pilot study using broader coagulation testing. We analysed the temporal pattern ...
journal_title：Thrombosis research
pub_type： 临床试验,杂志文章
doi：10.1016/j.thromres.2015.05.023
更新日期：2015-08-01 00:00:00
abstract：BACKGROUND:The CHADS2 and CHA2DS2-VASc scores are well-established clinical scales to estimate the risk of stroke in patients with atrial fibrillation (AF). However, the predictive power of the two scales concerning left atrial thrombus (LAT) or spontaneous echo contrast (SEC) has not been well investigated. Therefore,...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2020.07.055
更新日期：2020-11-01 00:00:00
abstract：:We tested whether inhibition of platelet activation by nitric oxide (NO) might be altered by strenuous exercise. Sixteen healthy male non-smokers, aged 20-26 years, underwent treadmill testing. All subjects reached Bruce stage IV without chest pain or abnormal ST-T wave changes. Platelet aggregation by Born's method a...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(97)00162-x
更新日期：1997-09-01 00:00:00
abstract：:Resveratrol (3, 4', 5-trihydroxystilbene), a natural polyphenol, is found in some plants that are used in human nutrition. Grapes are a major source for resveratrol, and a significant amount can also be found in red wine. Several experimental studies have demonstrated biological properties of resveratrol, especially i...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(02)00273-6
更新日期：2002-08-15 00:00:00
