Accumulation and release of acridine derivatives by intact platelets.

abstract：INTRODUCTION:Antithrombin deficiency is associated with increased risk of venous thromboembolism (VTE). We aimed to identify variants causing antithrombin deficiency in a Danish population. MATERIALS AND METHODS:We performed Sanger sequencing and, in relevant cases, multiplex ligation-dependent probe amplification ana...

journal_title：Thrombosis research

authors： Kjaergaard AD,Larsen OH,Hvas AM,Nissen PH

更新日期：2019-03-01 00:00:00

abstract：:Heparin is known to exert its antithrombotic effects by accelerating the effect of antithrombin (AT) and by mobilizing tissue factor pathway inhibitor (TFPI) into the circulation from vascular endothelium. Heparin treatment has been reported to decrease FVIIa activity by 40%; this was suggested as a new antithrombotic...

journal_title：Thrombosis research

authors： Hansen JB,Svensson B,Sandset PM,Thijssen F

更新日期：2000-12-01 00:00:00

abstract：:Four monoclonal antibodies have been produced, which are specific for the Ca2+ or Sr2(+)-induced conformation of human factor IX. Certain, but not all, gamma-carboxy-glutamic acid residues in factor IX are involved in the epitope expression together with the conformation stabilized by the adjacent region of Gla-domain...

journal_title：Thrombosis research

authors： Sugo T,Mizuguchi J,Kamikubo Y,Matsuda M

更新日期：1990-06-15 00:00:00

abstract：:Three commercial thrombin preparations used clinically to stop topical bleeding were studied. The specific activities of these preparations were 1,064 U/mg (human); 59 U/mg (bovine) and 147 U/mg (bovine). SDS-PAGE analysis of the human product produced one major band corresponding to a molecular weight of alpha-thromb...

journal_title：Thrombosis research

authors： Suzuki S,Sakuragawa N

更新日期：1989-02-01 00:00:00

abstract：:Acute coronary syndromes (ACS) represent the main clinical manifestation of atherosclerotic progression in the coronary district. Thrombosis plays a critical role in the patho-anatomical of ACS, as disruption of an atherosclerotic plaque exposes flowing blood to subendothelial collagen, tissue factor, and other procoa...

journal_title：Thrombosis research

authors： Abbate R,Cioni G,Ricci I,Miranda M,Gori AM

更新日期：2012-03-01 00:00:00

abstract：INTRODUCTION:Bleeding after cardiac surgery may be caused by surgical factors, impaired haemostasis, or a combination of both. Transfusion of blood products is used to improve haemostasis, but little is known about what combination is optimal. We hypothesized that addition of both fibrinogen and platelets to blood samp...

journal_title：Thrombosis research

authors： Shams Hakimi C,Fagerberg Blixter I,Hansson EC,Hesse C,Wallén H,Jeppsson A

更新日期：2014-10-01 00:00:00

abstract：BACKGROUND:Muromonab-CD3 is a murine monoclonal antibody (MoAb) that is used in the prophylaxis and treatment of acute graft rejection. Activation of coagulation and fibrinolysis following anti-CD3 administration have been reported in some patients to lead to irreversible intragraft thrombosis. DESIGN AND METHODS:We h...

journal_title：Thrombosis research

authors： Lozano M,Oppenheimer F,Cofan F,Rosinyol L,Mazzara R,Escolar G,Ordinas A

更新日期：2001-12-15 00:00:00

abstract：:An issue of great importance and continuing concern with regard to all products derived from human plasma is their safety from potential contaminants in the source material from which they are purified. Since viral contaminants are a major safety consideration with these products, a number of different methods, includ...

journal_title：Thrombosis research

authors： Chandra S,Groener A,Feldman F

更新日期：2002-03-01 00:00:00

abstract：:Isolated rat liver macrophages (Kupffer cells) produce a factor(s) which stimulates fibrinogen synthesis 5-8 fold in hepatocyte cultures. The production of the stimulatory factor reached maximum during the first 24 hours the cells were in culture. When the cell culture medium was chromatographed on a calibrated Sephad...

journal_title：Thrombosis research

authors： Sanders KD,Fuller GM

更新日期：1983-10-15 00:00:00

abstract：:Tissue factor, the cofactor for factor VIIa-catalyzed activation of factors IX and X, plays an important role in the initiation of hemostasis. However, the distribution of tissue factor in the body has not been defined until recently. In the present study frozen sections of non-malignant human tissues were immunostain...

journal_title：Thrombosis research

authors： Fleck RA,Rao LV,Rapaport SI,Varki N

更新日期：1990-07-15 00:00:00

abstract：:Eight patients with femoro-popliteal or sural DVT, confirmed by phlebography, were treated with intravenous Desmin (LMW-dermatan sulphate): on the first day, after an initial i.v. injection of 400 mg, all patients received an infusion of 800 mg in 500 ml of saline, during 24 hours; this infusion was repeated in each o...

journal_title：Thrombosis research

authors： Kretz JG,Chakfe N,Wiesel ML,Grunebaum L,Zamboni V,Palazzini E,Cazenave JP

更新日期：1996-12-15 00:00:00

abstract：OBJECTIVE:To date, only a few case studies have reported occurrence of thrombosis in patients with VonWillebrand disease (VWD). No studies have looked at its incidence in this patient population. The aim of this study was to test our hypothesis that decreased VonWillebrand factor (VWF) levels confer a protective effect...

journal_title：Thrombosis research

authors： Qureshi W,Hassan S,Dabak V,Kuriakose P

更新日期：2012-11-01 00:00:00

abstract：:An in vitro test system was developed to examine the effects of red blood cells (RBC) on shear-induced platelet adhesion (SIPAD) and platelet aggregation (SIPAG). Suspensions of human platelets labeled with Mepacrine and suspended in citrated plasma were exposed to single, continuous or repetitive (120-300x) one secon...

journal_title：Thrombosis research

authors： Joist JH,Bauman JE,Sutera SP

更新日期：1998-12-15 00:00:00

abstract：:Human saphenous veins were analyzed for presence of plasminogen activators (t-PA) in the endothelium of the vessel walls by immunohistochemical techniques. We used a polyclonal rabbit antihuman t-PA antibody, a monoclonal mouse-antihuman t-PA and a goat antibody to low molecular weight urokinase. To demonstrate presen...

journal_title：Thrombosis research

authors： Risberg B,Eriksson E,Björk S,Hansson GK

更新日期：1986-02-01 00:00:00

abstract：:Studies with animal models implicate the plasma proteases factor XIIa (FXIIa) and α-kallikrein in arterial and venous thrombosis. As congenital deficiencies of factor XII (FXII) or prekallikrein (PK), the zymogens of FXIIa and α-kallikrein respectively, do not cause bleeding disorders, inhibition of these enzymes may ...

journal_title：Thrombosis research

authors： Kokoye Y,Ivanov I,Cheng Q,Matafonov A,Dickeson SK,Mason S,Sexton DJ,Renné T,McCrae K,Feener EP,Gailani D

更新日期：2016-04-01 00:00:00

abstract：:We have developed a new bioassay for endothelium-derived relaxing factor (EDRF) or nitric oxide (NO) using human [3H]guanosine triphosphate (GTP)-labeled platelets. The labeled platelets were preincubated with isobutyl-methylxanthine and co-cultured with endothelial cells and the [3H]cyclic guanosine monophosphate (cG...

journal_title：Thrombosis research

authors： Kishi Y,Watanabe R,Ashikaga T,Numano F

更新日期：1995-06-15 00:00:00

abstract：AIM:Postprandial platelet activation was related to postprandial insulin rather than glucose levels in a previous meal insulin study in type 2 diabetes mellitus (T2DM). We therefore compared postprandial platelet activation in type 1 (T1DM) patients without insulin secretion and T2DM patients with high postprandial ins...

journal_title：Thrombosis research

authors： Spectre G,Stålesen R,Östenson CG,Hjemdahl P

更新日期：2016-05-01 00:00:00

abstract：:The effects of platelets on clot lysis and plasminogen activation by staphylokinase (SAK) were investigated. At concentrations ranging from 2 x 10(-7) to 1 x 10(-5)g/ml of SAK, the lysis time of platelet-rich plasma clots (PRP-clots) was shorter than that of platelet-poor plasma clots (PPP-clots). This reduction of cl...

journal_title：Thrombosis research

authors： Suehiro A,Tsujioka H,Yoshimoto H,Ueda M,Higasa S,Kakishita E

更新日期：1995-10-15 00:00:00

abstract：BACKGROUND:The usefulness of coagulation tests performed before epidural analgesia for surgery or to alleviate labour pain is controversial. The aims of this study were: (1) to evaluate the prevalence of abnormal tests in a large cohort of healthy pregnant women and their association with epidural hematoma; (2) to asse...

journal_title：Thrombosis research

authors： Franchi F,Ibrahim B,Rossi F,Maspero ML,Morabito O,Asti D,Bucciarelli P,Biguzzi E

更新日期：2011-07-01 00:00:00

abstract：BACKGROUND:Arterial calcification is associated with increased risk of cardiovascular events. Osteoprotegerin (OPG) is a cytokine involved in the bone metabolism and vascular calcification. Recent data support a relationship between high serum levels of OPG and increased risk for cardiovascular disease in human. The ai...

journal_title：Thrombosis research

authors： Guldiken B,Guldiken S,Turgut B,Turgut N,Demir M,Celik Y,Arikan E,Tugrul A

更新日期：2007-01-01 00:00:00

abstract：:A direct assay for plasminogen activator (PA) was developed. It employed polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and beta-mercaptoethanol to monitor PA-mediated conversion of single chain, 125I-plasminogen to two chain plasmin. By incorporating Triton X-100, albumin and trasylol in...

journal_title：Thrombosis research

authors： Mussoni L,Lawrence D,Loskutoff DJ

更新日期：1984-05-01 00:00:00

abstract：:Platelets from rats fed a diet high in linoleic acid (6%) bound increased amounts of fibrinogen on stimulation with ADP, compared to those from rats fed diets with low (2%) or no linoleic acid. However, this increased fibrinogen binding was associated with a decrease in platelet aggregation induced by ADP. Changes in ...

journal_title：Thrombosis research

authors： McGregor L,Toor B,McGregor JL,Renaud S,Clemetson KJ

更新日期：1984-04-01 00:00:00

abstract：:The time course and degree of collagen induced platelet shape change, aggregation and ATP-secretion in citrated human platelet rich plasma was monitored with a lumi-aggregometer. Onset of ATP-secretion was measurable within seconds after collagen challenge and always distinctly before onset of shape change. In absence...

journal_title：Thrombosis research

authors： Malmgren R

更新日期：1986-08-15 00:00:00

abstract：:Severe bleeding took place in a patient when abdominal aneurysm was removed by operation. Bleeding continued after infusion of heparin, antithrombin III (ATIII), fresh platelets and fresh blood. Infusion of tranexamic acid resulted in an immediate cessation of bleeding and improvement of his general condition. Sometim...

journal_title：Thrombosis research

authors： Takada A,Takada Y,Mori T,Sakaguchi S

更新日期：1990-04-15 00:00:00

abstract：BACKGROUND:Non-vitamin K antagonist oral anticoagulants (NOACs) do not need routine laboratory monitoring but measurement of drug concentration is important in emergency conditions. Specific laboratory tests are not readily available or not implemented in every hospital. Point-of-Care Tests (POCT) may bridge this gap a...

journal_title：Thrombosis research

authors： Padayattil Jose S,Banzato A,Carraro P,Haleh A,Rossi K,Nante G,Denas G,Zoppellaro G,Pengo V

更新日期：2018-03-01 00:00:00

abstract：:A rapid method is described for the production of a human marrow cell suspension highly enriched in megakaryocytes. These concentrates were incubated with radiolabelled amino-acids, and cell lysates were then analysed for fibrinogen synthesis. Neosynthesized proteins were detected by immunoprecipitation, immuno-affini...

journal_title：Thrombosis research

authors： Belloc F,Hourdille P,Fialon P,Boisseau MR,Soria J

更新日期：1985-05-15 00:00:00

abstract：:In this paper, we demonstrated that the procoagulant action of Lonomia achelous (Cramer) is due in part to a component that activates prothrombin. The activation by crude venom and Fractions obtained by gel filtration on Sephadex G-75 is not dependent of phospholipid, Ca++ or Factor V. The activation of prothrombin by...

journal_title：Thrombosis research

authors： Guerrero B,Arocha-Piñango CL

更新日期：1992-05-01 00:00:00

abstract：:Lupus anticoagulants (LA) are IgG or IgM antibodies which prolong phospholipid-dependent coagulation tests. For the detection and quantitation of such antibodies, we have developed an ELISA with cephalin as the coating antigen. The sensitivity of this assay was compared to the activated partial thromboplastin time (AP...

journal_title：Thrombosis research

authors： Sletnes KE,Keirung G,Wisløff F

更新日期：1990-01-15 00:00:00

abstract：:We have previously reported on a microassay based on human kininogens as cysteine proteinase inhibitors (CPIs), which could quantify partially purified kininogens from different biological fluids (J Pharmacol Meth 26, 113-124, 1991). In the present study we describe a functional method that, when assuming a 1:1 stoich...

journal_title：Thrombosis research

authors： Karlsrud TS,Buø L,Aasen AO,Johansen HT

更新日期：1996-05-01 00:00:00

abstract：:Normal haemostasis requires maintenance of a careful equilibrium between the necessity to clot when bleeding and the retention of fluid phase at all other times. Disruption of this equilibrium can result in catastrophic outcomes, e.g. acute myocardial infarction and pulmonary embolism. However, despite the significant...

journal_title：Thrombosis research

authors： Lim HY,O'Malley C,Donnan G,Nandurkar H,Ho P

更新日期：2019-07-01 00:00:00