Abstract：
BACKGROUND:Muromonab-CD3 is a murine monoclonal antibody (MoAb) that is used in the prophylaxis and treatment of acute graft rejection. Activation of coagulation and fibrinolysis following anti-CD3 administration have been reported in some patients to lead to irreversible intragraft thrombosis. DESIGN AND METHODS:We have studied the effect of muromonab-CD3 infusion on platelets using flow cytometry in six patients who received three daily doses of muromonab-CD3 as prophylaxis of rejection before receiving a living donor renal transplant. Samples were collected before, 15 and 60 min after muromonab-CD3 infusion. Immunolabeling of platelets was performed in whole blood using dual-color analysis. The following conjugated MoAb were used: anti-CD41a, -CD36, -CD42b, -CD62P, -CD63, -factor V/Va and nonspecific Ig. Samples were analyzed with a FACScan flow cytometer (Becton Dickinson, Mountain View, CA, USA). RESULTS:After muromonab-CD3 infusion, an increase in the binding of MoAb anti-factor V/Va to platelets was seen, which was only statistically significant (2.2% vs. 12.8%, P=.04) after 15 min of the second dose. No significant changes were seen in the other MoAbs studied. No thrombotic complications were observed after transplantation. INTERPRETATION AND CONCLUSION:In uremic patients receiving muromonab-CD3 infusion as prophylaxis of graft rejection, an increase in the binding of anti-factor V/Va, denoting an increased exposure of anionic phospholipids in platelets, was seen. This increase in platelet procoagulant activity might contribute to the appearance of thromboses within renal graft seen in some patients who received muromonab-CD3.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Lozano M,Oppenheimer F,Cofan F,Rosinyol L,Mazzara R,Escolar G,Ordinas Adoi
10.1016/s0049-3848(01)00396-6subject
Has Abstractpub_date
2001-12-15 00:00:00pages
405-11issue
6eissn
0049-3848issn
1879-2472pii
S0049384801003966journal_volume
104pub_type
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更新日期：1987-12-15 00:00:00
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pub_type： 杂志文章
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journal_title：Thrombosis research
pub_type： 杂志文章
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更新日期：1985-01-01 00:00:00
