Abstract:
:It has become customary to regard the various glycerophospholipids as quite similar, and the acyl groups are considered to have little influence on the behaviour of the lipids in membranes or metabolism. Nevertheless, a number of recent observations by the authors and others indicate a high degree of metabolic compartmentation and substrate specificity with regard to the acyl substituents (acyl specificity) of glycerophospholipid metabolising enzymes in intact cells. 1. [32P]Orthophosphate and [3H]glycerol are incorporated into phosphatidylcholine (PC) and phosphatidylethanolamine (PE) of platelets and Swiss 3T3 fibroblasts with a [32P]/[3H]-ratio several fold lower than in glycerol-3-phosphate, phosphatidic acid (PA) and phosphatidylinositol (PI), suggesting distinct metabolic separation (probably by cellular compartmentation) of the glycerol and choline (or ethanolamine) branches of de novo phospholipid biosynthesis. 2. In fibroblasts the [32P]/[3H]-ratio varied 50-fold among the molecular species of PC, PE, PI and PA, which indicates that the enzymes involved in these conversions have some degree of acyl specificity. 3. In vitro assays for lipid-converting enzymes employ detergents, which affect acyl specificity of the enzymes (lipid kinases) both by their chemical nature and concentrations. 4. Thrombin stimulation of platelets causes formation of a multitude of diacylglycerol (DAG) molecular species, but only one major molecular species of PA is formed indicating that the DAG kinase may have distinct acyl specificity in the intact cell. 5. However, this specificity could also result from the net reactions of DAG kinase(s) and PA phosphohydrolase(s), which would constitute an ATP-utilising, paired regulation of the molecular species of PA and the inositol lipids on one hand, and PC, PE phosphatidylserine and triacylglycerol on the other. These findings indicate a high complexity of glycerophospholipid metabolism and a distinct acyl specificity in intact cells that are not apparent from studies in vitro. A major challenge for future research in this area is to bridge the apparent discrepancy between in vivo and in vitro observations regarding glycerophospholipid metabolism, an endeavour that will require more knowledge about the physical chemistry of naturally occurring molecular species than is available today. The most prevailing appreciation of glycerophospholipids among biological scientists to-day is that they can be distinguished functionally, topographically and metabolically only by their head groups and that they form the bilayer in biological membranes. Most of us know that the fatty acid in the sn-2 position is unsaturated and have been indoctrinated that the higher the degree of unsaturation, the greater the fluidity of the membrane.(ABSTRACT TRUNCATED AT 400 WORDS)
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Holmsen H,Hindenes JO,Fukami Mdoi
10.1016/0049-3848(92)90006-vsubject
Has Abstractpub_date
1992-08-01 00:00:00pages
313-23issue
3eissn
0049-3848issn
1879-2472pii
0049-3848(92)90006-Vjournal_volume
67pub_type
杂志文章,评审abstract::All the thrombolytic agents currently in clinical use act as plasminogen activators. In this study evidence is presented that also oxidants of the phagocyte type are of fibrinolytic efficiency in vivo. Activated phagocytes participate in physiologic fibrinolysis. The cells generate plasminogen activators and reactive ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(91)90189-4
更新日期:1991-05-01 00:00:00
abstract:INTRODUCTION:The epidemiology of tamoxifen and venous thromboembolism (VTE) is not well understood, and most data on tamoxifen toxicity are from adjuvant clinical trials. This study examined the relationship between the duration of tamoxifen use in female patients with breast cancer and the risk of VTE in a large popul...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2011.11.025
更新日期:2012-07-01 00:00:00
abstract:INTRODUCTION:Cytomegalovirus (CMV)-associated thrombosis has been reported sporadically in the medical literature until now. However, thrombosis incidence and its risk factors have never been studied in a cohort of patients with acute CMV infection. MATERIALS AND METHODS:A retrospective case-control study. Medical cha...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2010.09.006
更新日期:2010-12-01 00:00:00
abstract::A tritium-labelled low molecular weight heparin fragment with an average molecular weight of 4000-6000 (Fragmin), was fractionated into its high and low affinity forms for antithrombin. The fractions obtained were injected into rabbits, and the plasma half-life (t1/2) volume of distribution (Vd), area under the curve ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(87)90345-8
更新日期:1987-10-01 00:00:00
abstract::Hemophilia patients with inhibitors against FVIII/FIX present with a major challenge in the clinical practice. In the 1970s such patients were treated in association with emergency situations and essential surgery using huge amounts of FVIII/FIX concentrates often preceded by extracorporal adsorption of antibodies aga...
journal_title:Thrombosis research
pub_type: 历史文章,杂志文章,评审
doi:10.1016/j.thromres.2010.01.021
更新日期:2010-04-01 00:00:00
abstract::Diagnosis and risk stratification of patients with acute coronary syndromes remain extremely important in order to avoid unnecessary hospitalizations on the one hand, and to improve prognosis of these patients on the other. For diagnosis of acute coronary syndromes, an ECG should be obtained at rest, troponin T and I ...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/s0049-3848(01)00298-5
更新日期:2001-09-30 00:00:00
abstract:OBJECTIVES:To measure the variations of different parameters in the hemostatic system and to analyze their roles in the development of hemostatic disorder in patients with orthotopic liver transplantation (OLT) procedures routinely performed without venovenous bypass. METHODS:The blood coagulation and fibrinolysis par...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2007.10.002
更新日期:2008-01-01 00:00:00
abstract::Experimental disseminated intravascular coagulation (DIC) can be induced by 4-h sustained infusion of endotoxin in a dose of 100 mg/kg in rats. The experimental model of DIC in rats was used to study the preventive effect of dipyridamole against DIC. Before the infusion of endotoxin, 0.5, 5.0 or 50.0 mg/kg of dipyrida...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(83)90216-5
更新日期:1983-03-15 00:00:00
abstract:INTRODUCTION:Recombinant members of the vitamin K-dependent protein family (factors IX and VII and protein C) have become important pharmaceuticals in treatment of bleeding disorders and sepsis. However, because the in vivo gamma-carboxylation system in stable cell lines used for transfection has a limited capacity of ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2007.11.002
更新日期:2008-01-01 00:00:00
abstract:INTRODUCTION:Dabigatran (Dabi) is not routinely monitored. However, in emergency cases quantitative assessment is required and laboratories must provide suitable tests at all hours. Little is known about Dabi effects on thrombin generation. MATERIALS AND METHODS:Patient samples (n=241) were analyzed for functional Dab...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2015.04.032
更新日期:2015-07-01 00:00:00
abstract::In order to choose the proper method for evaluating the antithrombin activity in samples of chitosan polysulphate (CP) with different polymerization degrees and sulphation degrees, we estimated the ability of direct anticoagulants to depress the coagulability of recalcified sheep blood using the third international he...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(01)00266-3
更新日期:2001-06-01 00:00:00
abstract::Calcium accumulating vesicles were prepared from washed human platelets. Ca2+ uptake could be stimulated with calmodulin. The stimulation of Ca2+ uptake was not abolished by the adenylate cyclase inhibitor 2',5'-dideoxyadenosine, thus excluding enhanced cAMP formation as a possible cause for the calmodulin effect. Our...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(86)91697-x
更新日期:1986-02-15 00:00:00
abstract:OBJECTIVE:To explore the functional exercises of deep breathing and lower limb motions for the prevention of venous thrombosis in patients undergoing major surgery for gynecologic malignancy. METHODS:All eligible patients admitted to the gynecologic oncology unit were randomized into the study and control groups. A co...
journal_title:Thrombosis research
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.thromres.2019.04.013
更新日期:2019-06-01 00:00:00
abstract::D-dimer of human fibrin was used as antigen to obtain monoclonal antibodies (mAbs). We have obtained 16 hybridomas producing mAbs of different specificity. Only two of these mAbs inhibited fibrin polymerization. They are of the IgG-class. One mAb (II-4d) inhibited fibrin polymerization to 100% and another (II-3b) to 6...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2004.03.005
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND:Peripheral pulmonary artery thrombus (PPT) is common in the clinic. However, due to the lack of an ideal diagnostic tool, PPT cannot be quickly diagnosed and effectively treated at present. Optical coherence tomography (OCT) is a new intravascular imaging technique that is characterized by high image resolut...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2017.10.021
更新日期:2018-01-01 00:00:00
abstract:INTRODUCTION:Available data on fibrin clot properties and fibrinolysis in hyperthyroidism and hypothyroidism are inconsistent. Our objective was to assess the impact of effective treatment of hyper- and hypothyroidism on fibrin clot characteristics. MATERIAL AND METHODS:In a case-control study, ex vivo plasma fibrin c...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2014.05.041
更新日期:2014-08-01 00:00:00
abstract::Reproducible disseminated intravascular coagulation in rabbits was provoked by two intravenous injections 24 hours apart of endotoxin from Salmonella enteritidis. There were mild symptoms of DIC before the second injection which considerably increased thereafter. In detail there was a sharp drop of the platelet count ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(93)90029-n
更新日期:1993-02-01 00:00:00
abstract:INTRODUCTION:Early achievement of therapeutic INR leads to shorter hospital stay and lesser cost. Two warfarin initiation nomograms (10 mg nomogram and 5 mg nomogram) are widely used but it is not yet clear which one is better. They have been validated in the West but there are no studies from India. We undertook this ...
journal_title:Thrombosis research
pub_type: 临床试验,信件
doi:10.1016/j.thromres.2018.12.026
更新日期:2019-04-01 00:00:00
abstract::The susceptibility of von Willebrand factor /vWF/ to digestion by human neutrophil cathepsin G in highly purified FVIII/vWF concentrate /BHS/ and in cryoprecipitate has been studied. In contrast to human neutrophil elastase, cathepsin G inactivates and degrades vWF not only in BHS but also in cryoprecipitate. Fibrinog...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(91)90657-i
更新日期:1991-02-15 00:00:00
abstract:OBJECTIVE:In sepsis, activation of coagulation and inhibition of fibrinolysis lead to microvascular thrombosis. Thus, clot stability might be a critical issue in the development of multiple organ dysfunction syndrome. Activated FXIII (FXIIIa) forms stable fibrin clots by covalently cross-linking fibrin monomers. Theref...
journal_title:Thrombosis research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/j.thromres.2006.02.003
更新日期:2007-01-01 00:00:00
abstract::Human antithrombin III was demonstrated to bind plasminogen specifically in a time and concentration-dependent manner. The above binding was also confirmed using ligand western blot assays. The interaction of plasminogen was significantly (>90%) inhibited by lysine, indicating the involvement of kringles in binding an...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(00)00268-1
更新日期:2000-09-15 00:00:00
abstract:BACKGROUND:Venous thromboembolism (VTE) can be the first manifestation of cancer; however, the current incidence of malignancy in unselected patients with first unprovoked VTE needs to be confirmed. MATERIAL AND METHODS:Between March 1st, 2013 and February 28th, 2015 we included and followed-up all patients living in ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2018.02.151
更新日期:2018-04-01 00:00:00
abstract:UNLABELLED:Suboptimal gastrointestinal absorption is a problem for many direct thrombin inhibitors. The studies presented herein describe the new oral direct thrombin inhibitor H 376/95, a prodrug with two protecting residues added to the direct thrombin inhibitor melagatran. Absorption properties in vitro: H 376/95 is...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(00)00399-6
更新日期:2001-02-01 00:00:00
abstract:INTRODUCTION:Diabetes mellitus is characterised by hyperglycaemia that plays an important role in the pathogenesis of diabetic complications including accumulation of methylglyoxal (MG), a highly reactive alpha-dicarbonyl metabolite of glucose degradation pathways and increased generation of advanced glycation end prod...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2007.03.002
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:Clinical and epidemiological studies have associated selective COX-2 inhibitors with an increased risk of cardiovascular events. There are no clinical studies on the possible effects of these drugs on secondary hemostasis. The hypothesis for this study is that the use of selective COX-2 inhibitors could affe...
journal_title:Thrombosis research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/j.thromres.2009.01.017
更新日期:2009-06-01 00:00:00
abstract::Tissue factor, the cofactor for factor VIIa-catalyzed activation of factors IX and X, plays an important role in the initiation of hemostasis. However, the distribution of tissue factor in the body has not been defined until recently. In the present study frozen sections of non-malignant human tissues were immunostain...
journal_title:Thrombosis research
pub_type: 更正并重新发布的文章,杂志文章
doi:10.1016/0049-3848(90)90148-6
更新日期:1990-07-15 00:00:00
abstract::Hemorrhagic and thrombotic complications are common in the term and preterm infant. Limited information is currently available about neonatal platelet structure and function, and how these may predispose infants to bleeding problems. This study comparing platelet ultrastructure of 71 different term and preterm infants...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(94)90039-6
更新日期:1994-03-15 00:00:00
abstract::Although effective therapies for haemophilia have been available for decades, the prevention and treatment of joint disease remain major clinical concerns for all haemophilia patients. Early identification of joint disease is vital to initiate or modify treatment, and prevent arthropathy. However, there remains a need...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/j.thromres.2014.02.015
更新日期:2014-06-01 00:00:00
abstract::An insertion/deletion (4G/5G) polymorphism in what has been shown to be an enhancer/repressor binding site in the promoter region of the PAI-1 gene has been related to plasma PAI-1 activity. Transfection studies demonstrated increased interleukin-1 stimulated PAI-1 synthesis in cells containing the 4G sequence. To stu...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(98)00132-7
更新日期:1998-12-01 00:00:00
abstract:INTRODUCTION:Many antithrombotic drugs may have a deleterious effect on normal haemostasis leading to bleeding complications. The aim of this study was to determine if sub-therapeutic (low) doses of antithrombotic agents, when administered in combination, have enhanced efficacy without augmentation of bleeding time. M...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2005.11.015
更新日期:2006-01-01 00:00:00