Abstract:
:Lipoprotein(a) [Lp(a)] has been demonstrated to be an independent risk factor for coronary heart disease. However, the precise mechanism by which it contributes to the development of atherosclerosis remains unclear. P-selectin is one of the major adhesion molecules to mediate the interaction between monocytes, platelets and endothelial cells. Increased expression of P-selectin has been frequently found in atherosclerotic lesions. The present study assessed the effects of native and oxidized LDL (n-LDL and ox-LDL) and Lp(a) (n-Lp(a) and ox-Lp(a)) on the expression of P-selectin in cultured human umbilical vein endothelial cells (HUVECs). Cell ELISA was used to measure the expression of P-selectin in HUVECs and monocyte adhesion assay was used to detect whether the P-selectin expressed in HUVECs was functional. P-selectin mRNA expression in HUVECs was determined by Northern blot. Results showed that P-selectin protein expression was not influenced by n-LDL, but was moderately increased by ox-LDL and n-Lp(a). ox-Lp(a) was the most potent stimulus for P-selectin expression, increasing it in HUVECs by 144 +/- 10% at 10 nmol/l and 202 +/- 22% at 20 nmol/l. In addition, it showed that dose dependency induced monocyte adherence to endothelial cells after incubation of HUVECs with ox-Lp(a). Northern blot analysis demonstrated that the amount of P-selectin mRNA was markedly increased after treatment with ox-Lp(a) but not with n-LDL, ox-LDL and n-Lp(a). These results demonstrate that ox-Lp(a) can induce P-selectin expression in HUVECs, which may thereby influence the pathogenesis of athersclerosis.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Zhao SP,Xu DYdoi
10.1016/s0049-3848(00)00363-7subject
Has Abstractpub_date
2000-12-15 00:00:00pages
501-10issue
6eissn
0049-3848issn
1879-2472pii
S0049-3848(00)00363-7journal_volume
100pub_type
杂志文章abstract:INTRODUCTION:Flow cytometry (FCM) is the most commonly used method for detection of platelet-derived microparticles (PDMPs), but it is poorly standardized and mainly used for "bedside" analyses in fresh samples. If PDMPs could be analyzed in previously frozen samples it would increase the usefulness of the method. Howe...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2009.10.006
更新日期:2010-03-01 00:00:00
abstract:INTRODUCTION:Metastases are responsible for more than 90% of cancer related mortality. The hematogenous metastatic invasion is a complex process in which the endothelium plays a key role. Extravasation is a dynamic process involving remodeling and change in cell shape and in cytoskeleton whereby a series of strongly de...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/S0049-3848(16)30145-1
更新日期:2016-04-01 00:00:00
abstract:INTRODUCTION:Pregnancy has recently been described as a generalized intravascular inflammatory response to the conceptus. Total fibrinogen concentrations increase during pregnancy. The percentage high molecular weight fibrinogen (HMW-Fg) of the concentration total fibrinogen is known to increase during acute-phase cond...
journal_title:Thrombosis research
pub_type: 临床试验,杂志文章
doi:10.1016/j.thromres.2004.04.008
更新日期:2004-01-01 00:00:00
abstract:INTRODUCTION:Unicompartmental knee arthroplasty (UKA) has increased due to potential favourable complication rates when compared to total knee arthroplasty (TKA). Although venous thromboembolism (VTE) is a well-documented complication after TKA limited data is available after UKA and mostly presented as secondary findi...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2020.07.002
更新日期:2020-11-01 00:00:00
abstract::The three polypeptide chains of fibrinogen, A alpha, B beta and gamma chain, are synthesized on separate polysomes. Fully formed fibrinogen is a six chain, disulfide-linked, dimeric molecule with a molecular weight of 340kDa. Previous pulse-chase studies with L-35 S methionine using the human hepatocellular carcinoma ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(87)90290-8
更新日期:1987-04-15 00:00:00
abstract:BACKGROUND:The AMPLIFY trial found significantly lower major bleeding (MB) and similar recurrent venous thromboembolism (VTE) risks associated with apixaban vs warfarin among patients with VTE. OBJECTIVES:To compare MB, clinically-relevant non-major (CRNM) bleeding, and recurrent VTE risks among clinically-relevant su...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2020.11.039
更新日期:2020-12-08 00:00:00
abstract::The effects and mechanism of action of a phospholipase C (PLC) from Pseudomonas aeruginosa on human platelet rich plasma were examined to better understand the interaction of PLC with human platelets. PLC caused platelet aggregation in a concentration-dependent manner. Enzymatic activity of PLC was necessary for aggre...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(88)90115-6
更新日期:1988-09-01 00:00:00
abstract::Full-length Tissue Factor (flTF) - the obligatory co-factor for the serine protease (factor) VII/VIIa - serves as the initiator of blood coagulation. The flTF/VIIa complex triggers a sequence of proteolytic events that lead to the formation of a hemostatic plug. Aside from hemostatic maintenance, flTF can contribute t...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/j.thromres.2012.02.027
更新日期:2012-05-01 00:00:00
abstract:INTRODUCTION:Tumor necrosis factor-alpha (TNF-α) exerts a critical role in inflammatory events through two distinct receptors, TNFR1 and TNFR2. Platelets have been recognized as important inflammatory cells, but little is known about the effects of TNF-α on the platelet activity. OBJECTIVES:In the present study we hav...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2018.10.008
更新日期:2018-12-01 00:00:00
abstract::Advances in antithrombotic therapy began when traditional anticoagulant agents such as heparin and the vitamin K antagonists like Coumadin became commercially available in the 1940s and 1950s. Inherent limitations of these compounds, including the need for monitoring and multiple food and drug interactions (with couma...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/j.thromres.2008.08.010
更新日期:2008-01-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to investigate the effect of CYP2C19 polymorphism and co-therapy with esomeprazole on the antiplatelet efficacy of clopidogrel. BACKGROUND:The antiplatelet efficacy of clopidogrel depends on CYP2C19 polymorphism or the co-administration of some kind of proton pump inhibitor (PPI). ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2015.03.033
更新日期:2015-06-01 00:00:00
abstract:BACKGROUND:The "HERDOO2 rule" is a prospectively validated clinical decision rule used to identify low-risk women who can safely discontinue anticoagulants after completing 5-12 months of anticoagulant treatment for unprovoked venous thromboembolism. The VIDAS®d-Dimer (DD) assay, a component of the rule, was used in th...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2018.07.020
更新日期:2018-09-01 00:00:00
abstract::Thromboangiitis obliterans (TAO), or Buerger's disease, is a segmental occlusive inflammatory disorder of the arteries and veins, and etiopathogenesis is still obscure. In the present study we investigated the prevalence of prothrombin 20210 G-->A, factor V 1691 G-->A (Factor V Leiden), and factor V 4070 A-->G (His 12...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(00)00310-8
更新日期:2000-11-01 00:00:00
abstract:INTRODUCTION:Remote ischaemic conditioning (RIC) has been shown to prevent platelet activation during ablation for atrial fibrillation. RIC has also been associated with more postoperative transfusion in the off-pump coronary artery bypass graft surgery (OPCAB) patients. We evaluated the effects of RIC on coagulation f...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2020.01.004
更新日期:2020-03-01 00:00:00
abstract::Adenosine is an endogenous antiaggregating substance that influences the platelet responses through specific A-type receptors that activate adenylate cyclase increasing the levels of 3',5'-cyclic adenosine monophosphate (cAMP). In this study, we investigated whether adenosine can also influence the levels of 3',5'-cyc...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(01)00412-1
更新日期:2002-01-01 00:00:00
abstract::A rapid method is described for the production of a human marrow cell suspension highly enriched in megakaryocytes. These concentrates were incubated with radiolabelled amino-acids, and cell lysates were then analysed for fibrinogen synthesis. Neosynthesized proteins were detected by immunoprecipitation, immuno-affini...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(85)90133-1
更新日期:1985-05-15 00:00:00
abstract::Using the standard turbidimetric method of platelet aggregation and quantitation of platelet secretion with 14C-Serotonin, we have examined the responsiveness of the platelets of mongrel dogs to arachidonic acid (AA), and the thromboxane agonist U46619 in the presence and absence of a subthreshold concentration of epi...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(89)90300-9
更新日期:1989-09-15 00:00:00
abstract:INTRODUCTION:The contribution of platelet activation to the pathogenesis of sickle cell disease (SCD) remains uncertain. We evaluated the safety and efficacy of eptifibatide, a synthetic peptide inhibitor of the αIIbβ3 receptor, in SCD patients during acute painful episodes. MATERIALS AND METHODS:In this single site, ...
journal_title:Thrombosis research
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.thromres.2013.08.002
更新日期:2013-09-01 00:00:00
abstract::To explore social aspects of asymptomatic carriership of factor V Leiden mutation (FVL) and how carriers have experienced procedure of screening for FVL, we have performed a qualitative study using semi-structured interviews. Seventeen carriers of FVL without history of venous thromboembolism (VTE) were interviewed. C...
journal_title:Thrombosis research
pub_type: 临床试验,杂志文章
doi:10.1016/j.thromres.2004.02.002
更新日期:2004-01-01 00:00:00
abstract:INTRODUCTION:Thrombotic storm (TS) presents as a severe, acute thrombotic phenotype, characterized by multiple clotting events and frequently affecting younger adults. Understanding the extensive hypercoagulation of an extreme phenotype as TS will also provide insight into the pathogenesis of a wider spectrum of thromb...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2017.11.016
更新日期:2018-01-01 00:00:00
abstract::To investigate the effects of new drugs on the prevention of venous thrombosis, the Wessler stasis model is extensively employed usually in the rabbit. In this model, a hypercoagulable state is achieved by the administration of either heterologous serum (often human serum) or an activated coagulation factor followed b...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(96)00023-0
更新日期:1996-02-15 00:00:00
abstract:BACKGROUND:It is well demonstrated that obesity is an independent risk factor for cardiovascular diseases. Recent studies have shown that obesity, insulin resistance and atherosclerosis are closely related phenomena in which low-grade inflammatory state and prothrombotic condition has pivotal roles. It has been shown t...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2005.12.019
更新日期:2007-01-01 00:00:00
abstract::Clinically unsuspected pulmonary embolism (UPE) is frequently diagnosed in cancer patients undergoing routine computed tomography scans for staging purposes or treatment response evaluation. The reported incidence of UPE ranges from 1% to 5% which probably represents an underestimation. A significant proportion of can...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/S0049-3848(14)50028-X
更新日期:2014-05-01 00:00:00
abstract::125I-fibronectin is incorporated into fibrin when blood plasma is clotted with thrombin. This process is dependent upon fibronectin concentration, temperature, and the presence of calcium ions. Fibronectin appears to be a very strong inhibitor of fibrin formation and influences both the thrombin clotting time and fibr...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(82)90308-5
更新日期:1982-09-01 00:00:00
abstract:INTRODUCTION:Though thrombin-activatable fibrinolysis inhibitor (TAFI) may contribute to hypercoagulability during pregnancy, limited data are available on the role of TAFI in women with recurrent fetal loss. MATERIAL/METHODS:We performed a case-control study aimed at evaluating any possible association between TAFI l...
journal_title:Thrombosis research
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.thromres.2011.09.029
更新日期:2012-08-01 00:00:00
abstract::Aggregation responses of platelet-rich plasma (PRP) from monocrotaline pyrrole (MCTP)-treated rats were examined to help elucidate the role of platelets in MCTP-induced pulmonary hypertension. PRP from rats treated one or four days earlier with MCTP (5 mg/kg, i.v.) or vehicle exhibited the same slope and maximum aggre...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(83)90168-8
更新日期:1983-11-01 00:00:00
abstract::The fibrinolytic system was evaluated in a patient with homozygous protein C deficiency as well as in several members of his family with a partial deficiency of this protein. Before anticoagulant therapy the patient showed skin lesions which quickly disappeared after administration of fresh plasma. After anticoagulant...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(86)90260-4
更新日期:1986-05-01 00:00:00
abstract::Stimulation of rat platelets with U46619 induced Ca2+ mobilization and platelet shape change, but aggregation and secretion were induced when platelets were stimulated with U46619 plus phorbol 12-myristate 13-acetate (PMA). However, stimulation of rabbit platelets with U46619 induced all the three platelet responses. ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(89)90171-0
更新日期:1989-10-15 00:00:00
abstract:BACKGROUND:Brain tumor patients have an increased risk of venous thromboembolism (VTE). An important role in cancer-related VTE has been suggested for tissue factor (TF), the main initiator of the coagulation cascade. We conducted a prospective cohort study to determine whether expression levels of TF in brain tumors a...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2012.09.020
更新日期:2013-02-01 00:00:00
abstract:INTRODUCTION:Data regarding the clinical relevance of pulmonary infarction (PI) in patients with pulmonary embolism (PE) are lacking. The aim of this study was to investigate the clinical features of PE patients with PI and the prognostic role of PI for PE patients. MATERIALS AND METHODS:Based on computed tomography s...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2012.03.012
更新日期:2012-09-01 00:00:00