Abstract：
:The effects and mechanism of action of a phospholipase C (PLC) from Pseudomonas aeruginosa on human platelet rich plasma were examined to better understand the interaction of PLC with human platelets. PLC caused platelet aggregation in a concentration-dependent manner. Enzymatic activity of PLC was necessary for aggregation since heat-denatured PLC had no effect on platelets. P-nitrophenolphosphorylcholine, a substrate for PLC, was unable to cause platelet aggregation and inhibited PLC-induced aggregation if added to platelets prior to the addition of PLC. In addition, phosphorylcholine, a product of PLC action on phospholipid substrates, was unable to aggregate platelets. When PLC was tested on the aggregation response to known aggregating agents such as ADP, epinephrine, collagen or ristocetin, no inhibitory effect was seen. Studies with aspirin or nordihydroguairetic acid (NDGA) indicated that the action of PLC was independent of the prostaglandin and lipooxygenase pathways, respectively. The studies described herein point to a novel pathway of platelet aggregation by P. aeruginosa PLC.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Coutinho IR,Berk RS,Mammen Edoi
10.1016/0049-3848(88)90115-6subject
Has Abstractpub_date
1988-09-01 00:00:00pages
495-505issue
5eissn
0049-3848issn
1879-2472pii
0049-3848(88)90115-6journal_volume
51pub_type
杂志文章abstract：:Bleeding events are a common adverse effect in oral anticoagulation that frequently depend on the duration and intensity of treatment. Major bleeding events, particularly intracerebral hemorrhages, are a serious complication in cases that require the rapid reversal of anticoagulation. Urgent correction of the coagulat...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/s0049-3848(99)00085-7
更新日期：1999-08-15 00:00:00
abstract：:In vitro exper iments showed that polymeric hydrogels containing covalently immobilized heparin affect the parameters of blood clotting system. This is not accounted for by the elution of heparin into the surrounding medium and this property of heparin-containing polymers is caused by specific interaction of immobiliz...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(82)90192-x
更新日期：1982-07-15 00:00:00
abstract：:We have investigated the role of platelet activating factor (PAF) in the pathogenesis of a murine model of traumatic shock using WEB 2086, a specific antagonist of PAF. WEB 2086 (0.5 mg/kg) significantly reversed the decrease in mean arterial blood pressure (MABP) induced by PAF (0.3 micrograms/kg) in anesthetized rat...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(89)90107-2
更新日期：1989-02-01 00:00:00
abstract：INTRODUCTION:Venous thromboembolism (VTE) occurs frequently in at-risk hospitalized patients, and prophylaxis of VTE is significantly underused. We sought to preliminarily assess the validity of Caprini risk assessment model, a famous individual VTE risk assessment model, in Chinese hospitalized patients with VTE. MAT...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2012.08.001
更新日期：2012-11-01 00:00:00
abstract：:125I-fibronectin is incorporated into fibrin when blood plasma is clotted with thrombin. This process is dependent upon fibronectin concentration, temperature, and the presence of calcium ions. Fibronectin appears to be a very strong inhibitor of fibrin formation and influences both the thrombin clotting time and fibr...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(82)90308-5
更新日期：1982-09-01 00:00:00
abstract：INTRODUCTION:To test the hypothesis that the platelet microparticle (PMP) and endothelial microparticle (EMP) may contribute to the hypercoagulability associated with microvascular thrombosis in patients with nontraumatc osteonecrosis of the femoral head (ONFH). MATERIALS AND METHODS:The study comprised 46 patients wh...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2008.04.001
更新日期：2008-01-01 00:00:00
abstract：:Thrombocytopenia is a common finding in cancer patients and can have different and/or multifactorial pathogenesis. While in solid tumors it occurs often as a consequence of chemotherapy treatment, it is frequently observed at diagnosis in patients with hematological malignancies being aggravated to a potentially life-...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2018.02.001
更新日期：2018-04-01 00:00:00
abstract：:The ability of the COA-SET Fibrin monomer (COA-SET FM) test to detect soluble fibrin was evaluated by comparing the results of the COA-SET FM test with fibrinopeptide A (FPA) determinations following thrombin incubation of plasma or whole blood. In addition, two semiquantitative tests (erythrocytes-agglutination test ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(90)90066-l
更新日期：1990-02-15 00:00:00
abstract：:Rabbit platelets pretreated with CV-3988 had reduced responsiveness to platelet activating factor(PAF) even after the platelets had been washed with a buffer. This was due to its tight binding to receptor sites for PAF, but not conformational changes of the receptor, because the platelets that were pretreated with CV-...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(87)90215-5
更新日期：1987-04-01 00:00:00
abstract：INTRODUCTION:Uremic bleeding frequently occurs in dialysis patients. Although its mechanism is not well characterized, acquired platelet dysfunction has been implicated in its pathogenesis. Skin bleeding time has been used to characterize platelet dysfunction in this population. However, the bleeding time is prone to e...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2006.02.013
更新日期：2007-01-01 00:00:00
abstract：:Intimal proliferation of smooth muscle cells is an important characteristic of developing atherosclerotic lesions and late occlusion of venous bypass grafts. Platelet-derived growth factor, released from aggregating platelets at sites of endothelial injury, has been suggested as a main factor responsible for intimal h...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(89)90042-x
更新日期：1989-04-15 00:00:00
abstract：:The brain and the placenta synthesize identical peptides and proteins, such as brain-derived neurotrophic factor, oxytocin, vascular endothelial growth factor, cortisol, and matrix metalloproteinases. Given the promiscuity between neurochemistry and the mechanism of placentation, it would be expected that mental disor...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/S0049-3848(15)50446-5
更新日期：2015-02-01 00:00:00
abstract：INTRODUCTION:Patients with stable coronary artery disease (CAD) are at risk of arterial thrombosis causing myocardial infarction. Detection of global haemostatic markers of hypercoagulability and hypofibrinolysis may be important for risk stratification and individualised treatment. We examined overall haemostatic pote...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2013.03.015
更新日期：2013-05-01 00:00:00
abstract：:Incubation of recombinant human tissue factor apoprotein (Apo-TF) with human plasma decreased the recalcified clotting time of this plasma in a time-and dose-dependent manner suggesting relipidation of the Apo-TF by plasma lipoproteins. Incubation of Apo-TF with purified preparations of human very low density, low den...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(90)90182-c
更新日期：1990-11-01 00:00:00
abstract：:The protease inhibitor, aprotinin, has been examined for its ability to inhibit urokinase and tissue-type plasminogen activators at pH 7.4 in assays utilizing pyroGlu-Gly-Arg-p-nitroanilide and H-D-Ile-Pro-Arg-p-nitroanilide as substrates, respectively. Aprotinin inhibited both two-chain low molecular weight urokinase...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(88)90252-6
更新日期：1988-03-15 00:00:00
abstract：OBJECTIVES:In this randomized management study, we examined the safety of withholding anticoagulation on the basis of negative impedance plethysmography (IPG) compared to negative contrast venography (CV) in symptomatic patients with a first episode of clinically suspected deep vein thrombosis (DVT), and we determined ...
journal_title：Thrombosis research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0049-3848(01)00222-5
更新日期：2001-04-01 00:00:00
abstract：INTRODUCTION:Von Willebrand Disease (VWD) is the most common inherited bleeding disorder. Measurement of von Willebrand factor (VWF) activity in plasma is often based on platelet agglutination stimulated by the ristocetin cofactor activity. Novel assays, based on latex beads with recombinant glycoprotein Ib instead of ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2015.01.027
更新日期：2015-04-01 00:00:00
abstract：:The need for anticoagulation in dialysis patients is common and the incidence of venous thromboembolism (VTE) and atrial fibrillation in this population is high. While direct data are lacking on the management of anticoagulation in dialysis patients, careful weighing of risks and benefits on the basis of evidence from...
journal_title：Thrombosis research
pub_type： 杂志文章,实务指引
doi：10.1016/j.thromres.2005.03.031
更新日期：2006-01-01 00:00:00
abstract：INTRODUCTION:Antithrombin deficiency is associated with increased risk of venous thromboembolism (VTE). We aimed to identify variants causing antithrombin deficiency in a Danish population. MATERIALS AND METHODS:We performed Sanger sequencing and, in relevant cases, multiplex ligation-dependent probe amplification ana...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2019.01.022
更新日期：2019-03-01 00:00:00
abstract：:The heparin-derived oligosaccharide C3 (C3) is currently underdevelopment for the prevention and treatment of vascular dementia and senile dementia of Alzheimer's type. C3 exhibits a molecular weight of 2200-2500 Da with a narrow distribution. The objective of the present study was to assess the pharmacodynamics and p...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2003.11.008
更新日期：2003-01-01 00:00:00
abstract：INTRODUCTION:Pseudoprolongation of activated partial thromboplastin time (APTT) is a serious problem in anticoagulation therapy for patients with high hematocrit, such as cyanotic congenital heart diseases. APTT pseudoprolongation occurs when APTT assay is performed using routinely used vacuum sampling tubes containing...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2007.08.004
更新日期：2008-01-01 00:00:00
abstract：:A new method, based on the observation that leukocytes contain PGI2-synthetase activity, was used to measure the effect of pharmacological levels of sulphinpyrazone on the TXA2 and PGI2 production in whole blood. Four human volunteers took 400 mg sulphinpyrazone twice daily for 5 1/2 days. Blood was drawn before the s...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(82)90033-0
更新日期：1982-10-01 00:00:00
abstract：:Bovine hide collagen dispersions were swollen in the pH range 1.6-7.0, treated with glutaraldehyde, and dialyzed to neutral pH. The intensity with which these collagens reacted with human platelets in plasma was studied by aggregometry and scanning electron microscopy. Collagen swollen at a pH below 4.25 +/- 0.30 and ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(89)90463-5
更新日期：1989-07-01 00:00:00
abstract：OBJECTIVES:Screening of patients with sepsis for disseminated intravascular coagulation (DIC) has been recommended in several guidelines. However, DIC screening is still not widely accepted as an essential component of sepsis management, partly because of a lack of evidence that DIC screening has an effect on mortality...
journal_title：Thrombosis research
pub_type： 杂志文章,多中心研究
doi：10.1016/j.thromres.2017.11.023
更新日期：2018-01-01 00:00:00
abstract：INTRODUCTION:Fibrin structure and cellular composition of thrombi profoundly affect the clinical outcomes in ischemic coronary and peripheral artery disease. Our study addressed the interrelations of structural features of thrombi and routinely measured laboratory parameters. MATERIALS AND METHODS:Thrombi removed by t...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2015.02.004
更新日期：2015-04-01 00:00:00
abstract：:Isolated rat liver macrophages (Kupffer cells) produce a factor(s) which stimulates fibrinogen synthesis 5-8 fold in hepatocyte cultures. The production of the stimulatory factor reached maximum during the first 24 hours the cells were in culture. When the cell culture medium was chromatographed on a calibrated Sephad...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(83)90025-7
更新日期：1983-10-15 00:00:00
abstract：INTRODUCTION:Apoptotic cell death is a highly regulated genetic program, which has been observed in mature megakaryocytes fragmenting into platelets. The clock gene Per2, a key component of core clock oscillator, was involved in affecting both cell cycle control and apoptosis. Thus, loss of Per2 function may be conside...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2010.11.025
更新日期：2011-02-01 00:00:00
abstract：:The role of protein kinase C (PKC) in platelet activation by thrombin was assessed using a PKC inhibitor Ro 31-7549/001 (R2) which, in vitro, shows more selectivity for PKC than other kinase inhibitors. During early (1.5 s) thrombin-induced platelet activation, when phosphorylation of 47 kDa protein (pleckstrin) and m...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(93)90008-c
更新日期：1993-01-01 00:00:00
abstract：INTRODUCTION:Though thrombin-activatable fibrinolysis inhibitor (TAFI) may contribute to hypercoagulability during pregnancy, limited data are available on the role of TAFI in women with recurrent fetal loss. MATERIAL/METHODS:We performed a case-control study aimed at evaluating any possible association between TAFI l...
journal_title：Thrombosis research
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.thromres.2011.09.029
更新日期：2012-08-01 00:00:00
abstract：UNLABELLED:Disturbances of coagulation and fibrinolysis have been reported in patients with chronic uremia. Studies of different coagulation and fibrinolysis parameters in regularly dialyzed patients have yielded conflicting results. Global fibrinolytic capacity (GFC) examines the function of the entire fibrinolytic sy...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2004.08.014
更新日期：2005-01-01 00:00:00
