The role of protein kinase C in the initial events of platelet activation by thrombin assessed with a selective inhibitor.

Abstract:

:The role of protein kinase C (PKC) in platelet activation by thrombin was assessed using a PKC inhibitor Ro 31-7549/001 (R2) which, in vitro, shows more selectivity for PKC than other kinase inhibitors. During early (1.5 s) thrombin-induced platelet activation, when phosphorylation of 47 kDa protein (pleckstrin) and myosin light chain by PKC and myosin light chain kinase, respectively, are most readily differentiated, R2 suppressed phosphorylation of pleckstrin more effectively than myosin light chain. R2-inhibited dense granule secretion (measured 0-10 s using quenched-flow techniques) with a dose dependency similar to that for inhibition of pleckstrin phosphorylation, supporting a role for PKC in this process. R2, at 0.5 microM inhibited 47 kDa protein phosphorylation by more than 60%, but had only minimal effects on the kinetics (0-3s) of ADP-induced primary aggregation. At this same concentration, R2 potentiated the thrombin-induced rise in cytosolic calcium during early (0-15 s) activation as measured in the presence or absence of external calcium. These data support the hypothesis that activation of PKC during early platelet function helps regulate cytosolic calcium levels by limiting calcium release into the cytosol.

journal_name

Thromb Res

journal_title

Thrombosis research

authors

Geanacopoulos M,Turner J,Bowling KE,Vandenberg SR,Gear AR

doi

10.1016/0049-3848(93)90008-c

subject

Has Abstract

pub_date

1993-01-01 00:00:00

pages

113-24

issue

1

eissn

0049-3848

issn

1879-2472

pii

0049-3848(93)90008-C

journal_volume

69

pub_type

杂志文章
  • Impaired clot lysis by rt-PA catalyzed mini-plasminogen activation.

    abstract::The fibrinolytic system contains a proenzyme plasminogen (Plg) which is converted to plasmin (Plm) by the action of Plg activators. Physiological Plg activators are: tissue-type plasminogen activator (t-PA) and urokinase-type plasminogen activator. Plg was shown to be further cleaved by leukocyte elastase producing se...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/s0049-3848(97)00099-6

    authors: Duboscq C,Genoud V,Parborell MF,Kordich LC

    更新日期:1997-06-15 00:00:00

  • Filter bleeding time: a new in vitro test of hemostasis. I. Evaluation in normal and thrombocytopenic subjects.

    abstract::Disadvantages of current bleeding time methods include poor reproducibility, limited repeatability, and at times scar formation. We report a new, in vitro technique which circumvents these problems. Venous blood is collected in sodium citrate, stored capped in a siliconized tube at 37 degrees C until use, and made to ...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/0049-3848(83)90011-7

    authors: Uchiyama S,Stropp JQ,Claypool DA,Didisheim P,Dewanjee MK

    更新日期:1983-07-01 00:00:00

  • Factors affecting the first recurrence of noncardioembolic ischemic stroke.

    abstract::Studies of the factors affecting the first recurrence of ischemic stroke have reported inconsistent findings. Types of initial stroke and the racial differences in study samples are among the explanations that may account for this inconsistency. The aims of this study were to estimate the cumulative recurrence rates o...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/s0049-3848(99)00146-2

    authors: Wu TH,Chen HH,Lee TK

    更新日期:2000-02-01 00:00:00

  • Effects of bortezomib on platelet aggregation and ATP release in human platelets, in vitro.

    abstract:INTRODUCTION:Proteasome inhibitor bortezomib (PS-341) has been the first proteasome inhibitor that has entered clinical trials with its antiproliferative and proapoptotic effects in patients with multiple myeloma. Recent studies indicate that proteasome inhibitors can be useful in prevention of experimental arterial th...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/j.thromres.2007.06.013

    authors: Avcu F,Ural AU,Cetin T,Nevruz O

    更新日期:2008-01-01 00:00:00

  • Abnormal Ca2+ homeostasis in platelets of patients with myeloproliferative disorders: low levels of Ca2+ influx and efflux across the plasma membrane and increased Ca2+ accumulation into the dense tubular system.

    abstract::Ca2+ influx and efflux in unstimulated platelets and Ca2+ uptake by simultaneously isolated two membrane fractions of platelets from patients with myeloproliferative disorders (MPD) have been investigated. In MPD, Ca2+ influx and efflux across the plasma membrane in unstimulated platelets were in equilibrium at signif...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/0049-3848(89)90372-1

    authors: Fujimoto T,Fujimura K,Kuramoto A

    更新日期:1989-01-15 00:00:00

  • Retrievable vena cava filters: key considerations.

    abstract::Retrievable filters are a new generation of inferior vena cava (IVC) filters and represent an attractive option because they may be either left in place permanently or safely retrieved after a quite long period when they become unnecessary. In this review the currently available literature regarding retrievable IVC fi...

    journal_title:Thrombosis research

    pub_type: 杂志文章,评审

    doi:10.1016/j.thromres.2007.06.017

    authors: Imberti D,Prisco D

    更新日期:2008-01-01 00:00:00

  • Identification of low responders to a 300-mg clopidogrel loading dose in patients undergoing coronary stenting.

    abstract:BACKGROUND:Although patients undergoing coronary stenting routinely receive dual antiplatelet treatment to reduce the risk of stent thrombosis, this undesired event still occurs. A suboptimal response to clopidogrel treatment (low responders) has been suggested to contribute to stent thrombosis. In the present study, p...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/j.thromres.2004.07.007

    authors: Angiolillo DJ,Fernandez-Ortiz A,Bernardo E,Ramírez C,Barrera-Ramirez C,Sabaté M,Hernández R,Moreno R,Escaned J,Alfonso F,Bañuelos C,Costa MA,Bass TA,Macaya C

    更新日期:2005-01-01 00:00:00

  • Purification and some properties of a protein obtained from normal human plasma which inhibits the platelet aggregation induced by thrombotic thrombocytopenic purpura plasma.

    abstract::Plasma from patients with thrombotic thrombocytopenic purpura (TTP) caused the aggregation of washed human platelets, which was inhibited by preincubation with normal plasma. Using salt fractionation, ion exchange chromatography, and preparative agarose gel electrophoresis, we purified a protein from normal plasma whi...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/0049-3848(84)90155-5

    authors: Lian EC,Mui PT,Siddiqui FA,Chiu LL

    更新日期:1984-01-01 00:00:00

  • Pharmacodynamics assessment of Bemiparin after multiple prophylactic and single therapeutic doses in adult and elderly healthy volunteers and in subjects with varying degrees of renal impairment.

    abstract:INTRODUCTION:Aging and renal impairment may prolong the half-life and lead to accumulation of low molecular weight heparins. Correct dosing is critical to prevent bleeding or thrombosis. MATERIALS AND METHODS:Open, parallel study. Healthy adult [n=13] and elderly (>65yrs) [n=12] volunteers; and subjects with mild (ClC...

    journal_title:Thrombosis research

    pub_type: 杂志文章,多中心研究

    doi:10.1016/j.thromres.2014.03.038

    authors: Rico S,Antonijoan RM,Ballester MR,Gutierro I,Ayani I,Martinez-Gonzalez J,Borrell M,Fontcuberta J,Gich I

    更新日期:2014-06-01 00:00:00

  • Response to antiplatelet therapy is independent of endogenous thrombin generation potential.

    abstract:BACKGROUND:Thrombin is the most potent platelet activator, and achieves rapid platelet activation even in the presence of antiplatelet therapy. Since activated platelets respond stronger to additional stimuli, the extent of endogenous thrombin generation may in part be responsible for the reported response variability ...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/j.thromres.2013.04.008

    authors: Gremmel T,Panzer S,Steiner S,Seidinger D,Koppensteiner R,Pabinger I,Kopp CW,Ay C

    更新日期:2013-07-01 00:00:00

  • The skin bleeding time test as a predictor of brain bleeding time in a rat model.

    abstract::Previous studies have indicated that the skin bleeding time test does not accurately reflect visceral bleeding time (BT). The present study examines the predictive value of the skin bleeding time for brain bleeding tendency. Sixteen Sprague-Dawley rats were divided into equal groups. The first group (controls) underwe...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/0049-3848(94)90282-8

    authors: MacDonald JD,Remington BJ,Rodgers GM

    更新日期:1994-12-15 00:00:00

  • The C677T polymorphism of the methylenetetrahydrofolate reductase gene is associated with the level of decrease on diastolic blood pressure in essential hypertension patients treated by angiotensin-converting enzyme inhibitor.

    abstract:OBJECTIVES:Elevated plasma homocysteine has been implicated as a risk factor for hypertension. C677T polymorphism in methylenetetrahydrofolate reductase gene (MTHFR) is a major determinant of hyperhomocysteinemia, which results in endothelial dysfunction. Angiotensin-converting enzyme (ACE) inhibitors appear to remedy ...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/j.thromres.2004.04.005

    authors: Jiang S,Hsu YH,Xu X,Xing H,Chen C,Niu T,Zhang Y,Peng S,Xu X

    更新日期:2004-01-01 00:00:00

  • Evaluation of platelet function and pharmacological platelet inhibition in patients with myeloproliferative disorders using multiple electrode aggregometry.

    abstract:BACKGROUND:The aim of this study was to describe platelet aggregation characteristics by multiple electrode aggregometry (MEA) and to evaluate MEA for its potential to detect platelet dysfunction and response to anti-aggregatory drugs in patients with myeloproliferative disorders (MPD). METHODS:We compared the platele...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/j.thromres.2010.06.014

    authors: Robier C,Neubauer M,Sternad H,Quehenberger F,Rainer F,Neumeister P

    更新日期:2010-09-01 00:00:00

  • Intensity of anticoagulation and survival in patients hospitalized with COVID-19 pneumonia.

    abstract:BACKGROUND:SARS-CoV-2 infection has noted derangements in coagulation markers along with significant thrombotic complications. Post-mortem examinations show severe endothelial injury and widespread thrombotic microangiopathy in the pulmonary vasculature. Early reports describing the use of prophylactic anticoagulation ...

    journal_title:Thrombosis research

    pub_type: 评论,信件

    doi:10.1016/j.thromres.2020.09.030

    authors: Hsu A,Liu Y,Zayac AS,Olszewski AJ,Reagan JL

    更新日期:2020-12-01 00:00:00

  • Genetic score based on high-risk genetic polymorphisms and early onset of ischemic heart disease in an Italian cohort of ischemic patients.

    abstract::Several single-nucleotide polymorphisms (SNPs) have been recognized as associated with ischemic heart disease (IHD) although the optimal set of risk genotypes has not be identified. This study aimed to examine whether identified high-risk SNPs are associated with early onset of IHD. In the GENOCOR study, 44 high-risk ...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/j.thromres.2014.03.006

    authors: Vecoli C,Adlerstein D,Shehi E,Bigazzi F,Sampietro T,Foffa I,Carpeggiani C,L'abbate A,Andreassi MG

    更新日期:2014-05-01 00:00:00

  • Enhancing effect of platelets on staphylokinase-mediated clot lysis and plasminogen activation.

    abstract::The effects of platelets on clot lysis and plasminogen activation by staphylokinase (SAK) were investigated. At concentrations ranging from 2 x 10(-7) to 1 x 10(-5)g/ml of SAK, the lysis time of platelet-rich plasma clots (PRP-clots) was shorter than that of platelet-poor plasma clots (PPP-clots). This reduction of cl...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/0049-3848(95)00159-o

    authors: Suehiro A,Tsujioka H,Yoshimoto H,Ueda M,Higasa S,Kakishita E

    更新日期:1995-10-15 00:00:00

  • Combination and comparison of two models in prognosis of pulmonary embolism: results from TUrkey Pulmonary Embolism Group (TUPEG) study.

    abstract:BACKGROUND:Clinical parameters, biomarkers and imaging-based risk stratification are widely accepted in pulmonary embolism(PE). The present study has investigated the prognostic role of simplified Pulmonary Embolism Severity Index (sPESI) score and the European Society of Cardiology (ESC) model. METHODS:This prospecti...

    journal_title:Thrombosis research

    pub_type: 杂志文章,多中心研究

    doi:10.1016/j.thromres.2014.02.032

    authors: Ozsu S,Ozlu T,Sentürk A,Uçar EY,Kırkıl G,Kadıoğlu EE,Altınsoy B,Saylan B,Selimoğlu HŞ,Dabak G,Tutar N,Uysal A,TUPEG Study Investigators.

    更新日期:2014-06-01 00:00:00

  • Induction of urokinase-type plasminogen activator by lipopolysaccharide in PC-3 human prostatic cancer cells.

    abstract::Urokinase-type plasminogen activator (uPA) plays a central role in many aspects of cellular regulation, such as fibrinolysis, cell migration, and metastasis. The uPA induction by inflammatory stimuli such as IL-1, TNFalpha, and lipopolysaccharide (LPS) has been reported in a number of human cells. We examined the effe...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/s0049-3848(99)00174-7

    authors: Ohta S,Niiya K,Sakuragawa N,Fuse H

    更新日期:2000-03-01 00:00:00

  • Influence of exogenous and endogenous tissue-type plasminogen activator on the lysability of clots in a plasma milieu in vitro.

    abstract::The fibrinolytic potential of tissue-type plasminogen activator (t-PA) either incorporated in a clot (endogenous) or added to the surrounding plasma (exogenous), was studied in an in vitro system consisting of 125I-labeled human plasma clots (200 microliters) immersed in human plasma (2 ml). Clot lysis was measured as...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/0049-3848(84)90364-5

    authors: Zamarron C,Lijnen HR,Collen D

    更新日期:1984-08-01 00:00:00

  • Risk of deep venous thrombosis (DVT) in bedridden or wheelchair-bound multiple sclerosis patients: a prospective study.

    abstract:BACKGROUND:Multiple sclerosis (MS) often causes progressive loss of mobility, leading to limb paralysis. Venous and lymphatic stasis is a risk condition for venous thromboembolism (VTE). There is, however, no data on the frequency of VTE complicating the progression of MS. The aim of this study was to assess the freque...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/j.thromres.2009.06.023

    authors: Arpaia G,Bavera PM,Caputo D,Mendozzi L,Cavarretta R,Agus GB,Milani M,Ippolito E,Cimminiello C

    更新日期:2010-04-01 00:00:00

  • Real-world incidence of cancer following a first unprovoked venous thrombosis: Results from the EPIGETBO study.

    abstract:BACKGROUND:Venous thromboembolism (VTE) can be the first manifestation of cancer; however, the current incidence of malignancy in unselected patients with first unprovoked VTE needs to be confirmed. MATERIAL AND METHODS:Between March 1st, 2013 and February 28th, 2015 we included and followed-up all patients living in ...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/j.thromres.2018.02.151

    authors: Delluc A,Ianotto JC,Tromeur C,De Moreuil C,Couturaud F,Lacut K,Le Moigne E,Louis P,Thereaux J,Metges JP,Mottier D

    更新日期:2018-04-01 00:00:00

  • Neutropenia fails to prevent the acute phase stimulation of fibrinogen synthesis.

    abstract::This study evaluates the role of neutrophil granulocytes in mediating acute phase stimulation of fibrinogen synthesis. Turpentine was administered to neutropenic and non-neutropenic rats and fibrinogen synthetic rates measured in an isolated liver perfusion system. Using the (14C) carbonate technique for the measureme...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/0049-3848(82)90032-9

    authors: Kernoff L,Colman J

    更新日期:1982-10-01 00:00:00

  • Very low doses of unfractionated heparin potentiate the anti-Xa activity of low molecular weight heparin (enoxaparin)

    abstract::Some previous reports indicated that a minimal amount of anti-IIa is necessary for the optimal anti-Xa activity of low molecular weight heparin (LMW-H). To know if we could improve the prophylactic activity of LMW-H, providing a mild antithrombin effect, we conducted an experiment in which we administered subcutaneous...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/s0049-3848(97)00010-8

    authors: Pèrez-Requejo JL,Lucena-Solano O,Pérez-García M,Santarelli MT

    更新日期:1997-02-01 00:00:00

  • The presence of gamma' chain impairs fibrin polymerization.

    abstract:INTRODUCTION:A fraction of fibrinogen molecules contain an alternatively spliced variant chain called gamma'. Plasma levels of this variant have been associated with both myocardial infarction and venous thrombosis. Because clot structure has been associated with cardiovascular risk, we examined the effect of gamma' ch...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/j.thromres.2008.11.016

    authors: Gersh KC,Nagaswami C,Weisel JW,Lord ST

    更新日期:2009-07-01 00:00:00

  • Sphingosine inhibits factor Xa-catalyzed prothrombin activation on the surface of cultured calf pulmonary artery endothelium perturbed by hydrogen peroxide.

    abstract::The effects of hydrogen peroxide on factor Xa-catalyzed prothrombin activation on the surface of vascular endothelial cells, and the inhibitory effect of sphingosine on prothrombin activation were studied in cultured calf pulmonary artery endothelial cells (CPAEC). Hydrogen peroxide enhanced factor Xa-catalyzed prothr...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/0049-3848(92)90263-a

    authors: Tanabe S,Tamaki T,Wada Y

    更新日期:1992-07-01 00:00:00

  • An alternative pathway for fibrinolysis is activated in patients who have undergone cardiopulmonary bypass surgery and major abdominal surgery.

    abstract:INTRODUCTION:We conducted this prospective study in order to investigate the hypotheses that an alternative pathway for fibrinolysis is activated in patients who have undergone cardiopulmonary bypass (CPB) surgery and major abdominal surgery and that the levels of fibrin degradation products digested by polymorphonucle...

    journal_title:Thrombosis research

    pub_type: 临床试验,杂志文章

    doi:10.1016/j.thromres.2006.07.009

    authors: Gando S,Kameue T,Sawamura A,Hayakawa M,Hoshino H,Kubota N

    更新日期:2007-01-01 00:00:00

  • Analysis of serum cancer procoagulant activity and its possible use as a tumor marker.

    abstract::The two objectives of the study were to determine whether a procedure could be developed for measuring cancer procoagulant (CP) activity in human serum and if this procedure provided a method for distinguishing people with cancer from those without cancer. A procedure was developed for processing human serum such that...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/0049-3848(89)90256-9

    authors: Gordon SG,Benson B

    更新日期:1989-11-01 00:00:00

  • Thrombo-hemorrhagic deaths in acute promyelocytic leukemia.

    abstract::Acute promyelocytic leukemia (APL) has become the most curable form of acute myeloid leukemia after the advent of all-trans retinoic acid (ATRA). However, early deaths (ED) mostly due to the disease-associated coagulopathy remain the major cause of treatment failure. In particular, hemorrhagic events account for 40-65...

    journal_title:Thrombosis research

    pub_type: 杂志文章,评审

    doi:10.1016/S0049-3848(14)50019-9

    authors: Breccia M,Lo Coco F

    更新日期:2014-05-01 00:00:00

  • Echocardiographic evaluation for pulmonary hypertension after recurrent pulmonary embolism.

    abstract:INTRODUCTION:Although recurrent venous thromboembolism is a known risk factor for chronic thromboembolic pulmonary hypertension (CTEPH), the prevalence of CTEPH after recurrent pulmonary embolism (PE) is not clear. MATERIALS AND METHODS:A cohort screening study was performed to clarify the prevalence of echocardiograp...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/j.thromres.2011.07.045

    authors: Berghaus TM,Barac M,von Scheidt W,Schwaiblmair M

    更新日期:2011-12-01 00:00:00

  • C-terminal peptide alcohol, acid and amide analogs of desulfato hirudin54-65 as antithrombin agents.

    abstract::Analogs of the antithrombin peptide hirudin54-65 with C-terminal modifications have been synthesized in order to examine the requirements for alpha-thrombin inhibition. The C-terminal residue, Gln65, could be replaced with L-amino acids or amino alcohols with neutral or charged hydrophilic side chains without greatly ...

    journal_title:Thrombosis research

    pub_type: 杂志文章

    doi:10.1016/0049-3848(89)90090-x

    authors: Krstenansky JL,Payne MH,Owen TJ,Yates MT,Mao SJ

    更新日期:1989-05-15 00:00:00