Abstract:
:The role of protein kinase C (PKC) in platelet activation by thrombin was assessed using a PKC inhibitor Ro 31-7549/001 (R2) which, in vitro, shows more selectivity for PKC than other kinase inhibitors. During early (1.5 s) thrombin-induced platelet activation, when phosphorylation of 47 kDa protein (pleckstrin) and myosin light chain by PKC and myosin light chain kinase, respectively, are most readily differentiated, R2 suppressed phosphorylation of pleckstrin more effectively than myosin light chain. R2-inhibited dense granule secretion (measured 0-10 s using quenched-flow techniques) with a dose dependency similar to that for inhibition of pleckstrin phosphorylation, supporting a role for PKC in this process. R2, at 0.5 microM inhibited 47 kDa protein phosphorylation by more than 60%, but had only minimal effects on the kinetics (0-3s) of ADP-induced primary aggregation. At this same concentration, R2 potentiated the thrombin-induced rise in cytosolic calcium during early (0-15 s) activation as measured in the presence or absence of external calcium. These data support the hypothesis that activation of PKC during early platelet function helps regulate cytosolic calcium levels by limiting calcium release into the cytosol.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Geanacopoulos M,Turner J,Bowling KE,Vandenberg SR,Gear ARdoi
10.1016/0049-3848(93)90008-csubject
Has Abstractpub_date
1993-01-01 00:00:00pages
113-24issue
1eissn
0049-3848issn
1879-2472pii
0049-3848(93)90008-Cjournal_volume
69pub_type
杂志文章abstract::The fibrinolytic system contains a proenzyme plasminogen (Plg) which is converted to plasmin (Plm) by the action of Plg activators. Physiological Plg activators are: tissue-type plasminogen activator (t-PA) and urokinase-type plasminogen activator. Plg was shown to be further cleaved by leukocyte elastase producing se...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(97)00099-6
更新日期:1997-06-15 00:00:00
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journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(83)90011-7
更新日期:1983-07-01 00:00:00
abstract::Studies of the factors affecting the first recurrence of ischemic stroke have reported inconsistent findings. Types of initial stroke and the racial differences in study samples are among the explanations that may account for this inconsistency. The aims of this study were to estimate the cumulative recurrence rates o...
journal_title:Thrombosis research
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更新日期:2000-02-01 00:00:00
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journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2007.06.013
更新日期:2008-01-01 00:00:00
abstract::Ca2+ influx and efflux in unstimulated platelets and Ca2+ uptake by simultaneously isolated two membrane fractions of platelets from patients with myeloproliferative disorders (MPD) have been investigated. In MPD, Ca2+ influx and efflux across the plasma membrane in unstimulated platelets were in equilibrium at signif...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(89)90372-1
更新日期:1989-01-15 00:00:00
abstract::Retrievable filters are a new generation of inferior vena cava (IVC) filters and represent an attractive option because they may be either left in place permanently or safely retrieved after a quite long period when they become unnecessary. In this review the currently available literature regarding retrievable IVC fi...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/j.thromres.2007.06.017
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND:Although patients undergoing coronary stenting routinely receive dual antiplatelet treatment to reduce the risk of stent thrombosis, this undesired event still occurs. A suboptimal response to clopidogrel treatment (low responders) has been suggested to contribute to stent thrombosis. In the present study, p...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2004.07.007
更新日期:2005-01-01 00:00:00
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journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(84)90155-5
更新日期:1984-01-01 00:00:00
abstract:INTRODUCTION:Aging and renal impairment may prolong the half-life and lead to accumulation of low molecular weight heparins. Correct dosing is critical to prevent bleeding or thrombosis. MATERIALS AND METHODS:Open, parallel study. Healthy adult [n=13] and elderly (>65yrs) [n=12] volunteers; and subjects with mild (ClC...
journal_title:Thrombosis research
pub_type: 杂志文章,多中心研究
doi:10.1016/j.thromres.2014.03.038
更新日期:2014-06-01 00:00:00
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journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2013.04.008
更新日期:2013-07-01 00:00:00
abstract::Previous studies have indicated that the skin bleeding time test does not accurately reflect visceral bleeding time (BT). The present study examines the predictive value of the skin bleeding time for brain bleeding tendency. Sixteen Sprague-Dawley rats were divided into equal groups. The first group (controls) underwe...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(94)90282-8
更新日期:1994-12-15 00:00:00
abstract:OBJECTIVES:Elevated plasma homocysteine has been implicated as a risk factor for hypertension. C677T polymorphism in methylenetetrahydrofolate reductase gene (MTHFR) is a major determinant of hyperhomocysteinemia, which results in endothelial dysfunction. Angiotensin-converting enzyme (ACE) inhibitors appear to remedy ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2004.04.005
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND:The aim of this study was to describe platelet aggregation characteristics by multiple electrode aggregometry (MEA) and to evaluate MEA for its potential to detect platelet dysfunction and response to anti-aggregatory drugs in patients with myeloproliferative disorders (MPD). METHODS:We compared the platele...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2010.06.014
更新日期:2010-09-01 00:00:00
abstract:BACKGROUND:SARS-CoV-2 infection has noted derangements in coagulation markers along with significant thrombotic complications. Post-mortem examinations show severe endothelial injury and widespread thrombotic microangiopathy in the pulmonary vasculature. Early reports describing the use of prophylactic anticoagulation ...
journal_title:Thrombosis research
pub_type: 评论,信件
doi:10.1016/j.thromres.2020.09.030
更新日期:2020-12-01 00:00:00
abstract::Several single-nucleotide polymorphisms (SNPs) have been recognized as associated with ischemic heart disease (IHD) although the optimal set of risk genotypes has not be identified. This study aimed to examine whether identified high-risk SNPs are associated with early onset of IHD. In the GENOCOR study, 44 high-risk ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2014.03.006
更新日期:2014-05-01 00:00:00
abstract::The effects of platelets on clot lysis and plasminogen activation by staphylokinase (SAK) were investigated. At concentrations ranging from 2 x 10(-7) to 1 x 10(-5)g/ml of SAK, the lysis time of platelet-rich plasma clots (PRP-clots) was shorter than that of platelet-poor plasma clots (PPP-clots). This reduction of cl...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(95)00159-o
更新日期:1995-10-15 00:00:00
abstract:BACKGROUND:Clinical parameters, biomarkers and imaging-based risk stratification are widely accepted in pulmonary embolism(PE). The present study has investigated the prognostic role of simplified Pulmonary Embolism Severity Index (sPESI) score and the European Society of Cardiology (ESC) model. METHODS:This prospecti...
journal_title:Thrombosis research
pub_type: 杂志文章,多中心研究
doi:10.1016/j.thromres.2014.02.032
更新日期:2014-06-01 00:00:00
abstract::Urokinase-type plasminogen activator (uPA) plays a central role in many aspects of cellular regulation, such as fibrinolysis, cell migration, and metastasis. The uPA induction by inflammatory stimuli such as IL-1, TNFalpha, and lipopolysaccharide (LPS) has been reported in a number of human cells. We examined the effe...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(99)00174-7
更新日期:2000-03-01 00:00:00
abstract::The fibrinolytic potential of tissue-type plasminogen activator (t-PA) either incorporated in a clot (endogenous) or added to the surrounding plasma (exogenous), was studied in an in vitro system consisting of 125I-labeled human plasma clots (200 microliters) immersed in human plasma (2 ml). Clot lysis was measured as...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(84)90364-5
更新日期:1984-08-01 00:00:00
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journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2009.06.023
更新日期:2010-04-01 00:00:00
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journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2018.02.151
更新日期:2018-04-01 00:00:00
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journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(82)90032-9
更新日期:1982-10-01 00:00:00
abstract::Some previous reports indicated that a minimal amount of anti-IIa is necessary for the optimal anti-Xa activity of low molecular weight heparin (LMW-H). To know if we could improve the prophylactic activity of LMW-H, providing a mild antithrombin effect, we conducted an experiment in which we administered subcutaneous...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(97)00010-8
更新日期:1997-02-01 00:00:00
abstract:INTRODUCTION:A fraction of fibrinogen molecules contain an alternatively spliced variant chain called gamma'. Plasma levels of this variant have been associated with both myocardial infarction and venous thrombosis. Because clot structure has been associated with cardiovascular risk, we examined the effect of gamma' ch...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2008.11.016
更新日期:2009-07-01 00:00:00
abstract::The effects of hydrogen peroxide on factor Xa-catalyzed prothrombin activation on the surface of vascular endothelial cells, and the inhibitory effect of sphingosine on prothrombin activation were studied in cultured calf pulmonary artery endothelial cells (CPAEC). Hydrogen peroxide enhanced factor Xa-catalyzed prothr...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(92)90263-a
更新日期:1992-07-01 00:00:00
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journal_title:Thrombosis research
pub_type: 临床试验,杂志文章
doi:10.1016/j.thromres.2006.07.009
更新日期:2007-01-01 00:00:00
abstract::The two objectives of the study were to determine whether a procedure could be developed for measuring cancer procoagulant (CP) activity in human serum and if this procedure provided a method for distinguishing people with cancer from those without cancer. A procedure was developed for processing human serum such that...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(89)90256-9
更新日期:1989-11-01 00:00:00
abstract::Acute promyelocytic leukemia (APL) has become the most curable form of acute myeloid leukemia after the advent of all-trans retinoic acid (ATRA). However, early deaths (ED) mostly due to the disease-associated coagulopathy remain the major cause of treatment failure. In particular, hemorrhagic events account for 40-65...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/S0049-3848(14)50019-9
更新日期:2014-05-01 00:00:00
abstract:INTRODUCTION:Although recurrent venous thromboembolism is a known risk factor for chronic thromboembolic pulmonary hypertension (CTEPH), the prevalence of CTEPH after recurrent pulmonary embolism (PE) is not clear. MATERIALS AND METHODS:A cohort screening study was performed to clarify the prevalence of echocardiograp...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2011.07.045
更新日期:2011-12-01 00:00:00
abstract::Analogs of the antithrombin peptide hirudin54-65 with C-terminal modifications have been synthesized in order to examine the requirements for alpha-thrombin inhibition. The C-terminal residue, Gln65, could be replaced with L-amino acids or amino alcohols with neutral or charged hydrophilic side chains without greatly ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(89)90090-x
更新日期:1989-05-15 00:00:00