Abstract：
:The proteinase, brinase (Mr approximately 35000), from Aspergillus oryzae, which has been used in therapeutic attempts as a thrombolytic agent in arterial thrombosis, binds to purified human alpha 2-macroglobulin (alpha 2M) with a stoichiometry of 1.7-1.9 mol of enzyme/mol inhibitor. This binding leads to quantitative cleavage of the bait region of the inhibitor and to release of 3.6 thiol groups per molecule of alpha 2M, reflecting cleavage of the thioester bonds. The reaction with brinase is accompanied by a similar conformational change of alpha 2M as the reaction with trypsin, as shown by gradient gel electrophoresis and spectroscopic analyses. Brinase thus binds to alpha 2M in a similar manner as most small proteinases. However, in the complex formed at saturation of alpha 2M with brinase, the enzyme retains considerable proteolytic activity against macromolecular substrates, corresponding to about 25% of that of the free enzyme with fibrin as substrate. This finding indicates that the trapping of brinase by alpha 2M is less efficient than that of smaller proteinases. The complex formed at equimolar concentrations of the reactants has appreciably lower, although still significant, activity, amounting to 5-10% of that of free brinase against fibrin. This proteolytic activity of alpha 2M-brinase complexes against high-molecular-weight substrates most likely accounts for the thrombolytic effect of brinase in vivo. The observations also indicate that this thrombolytic activity increases more than proportionally to the brinase concentration as the latter is increased to approach saturation of alpha 2M in plasma.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Larsson LJ,Frisch EP,Törneke K,Lindblom T,Björk Idoi
10.1016/0049-3848(88)90359-3subject
Has Abstractpub_date
1988-01-01 00:00:00pages
55-68issue
1eissn
0049-3848issn
1879-2472journal_volume
49pub_type
杂志文章abstract：:The association between blood coagulation and cancer growth and metastatic dissemination is not yet completely understood. In this study we demonstrate that thrombin is capable of enhancing tumor cell adhesive properties and thereby increases tumor cell metastatic potential. Following exposure to alpha-thrombin, Walke...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(92)90081-k
更新日期：1992-11-01 00:00:00
abstract：:Severe prekallikrein (Fletcher factor) deficiency was diagnosed in a 49-year-old woman and in 3 of her siblings. Functional prekallikrein (PKK) activity was found below 1% by clotting assay and 20% by amidolytic assay in all the affected subjects; PKK cross-reacting material (CRM) was present in all the patients (anti...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(90)90222-x
更新日期：1990-12-01 00:00:00
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journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2016.05.012
更新日期：2016-07-01 00:00:00
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journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2004.03.009
更新日期：2004-01-01 00:00:00
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journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2013.01.036
更新日期：2013-08-01 00:00:00
abstract：:The ability of the COA-SET Fibrin monomer (COA-SET FM) test to detect soluble fibrin was evaluated by comparing the results of the COA-SET FM test with fibrinopeptide A (FPA) determinations following thrombin incubation of plasma or whole blood. In addition, two semiquantitative tests (erythrocytes-agglutination test ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(90)90066-l
更新日期：1990-02-15 00:00:00
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journal_title：Thrombosis research
pub_type： 临床试验,杂志文章
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更新日期：2015-08-01 00:00:00
abstract：:A series of 8 analogues of platelet-activating factor (PAF) in which both the acetyl group and the polar head are changed, were tested for biological activities on human and rabbit platelets in plasma. Two of these compounds, 1-O-hexadecyl-2-O-ethyl-rac-glycero-3-phosphoric acid-5'-trimethylammoniumpentyl ester and 1-...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(88)90244-7
更新日期：1988-02-15 00:00:00
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pub_type： 杂志文章,多中心研究
doi：10.1016/j.thromres.2011.08.031
更新日期：2012-05-01 00:00:00
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journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2020.10.015
更新日期：2020-12-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0049-3848(94)90282-8
更新日期：1994-12-15 00:00:00
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journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2004.06.031
更新日期：2004-01-01 00:00:00
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journal_title：Thrombosis research
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：10.1016/s0049-3848(99)00110-3
更新日期：1999-11-15 00:00:00
abstract：:The two objectives of the study were to determine whether a procedure could be developed for measuring cancer procoagulant (CP) activity in human serum and if this procedure provided a method for distinguishing people with cancer from those without cancer. A procedure was developed for processing human serum such that...
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pub_type： 杂志文章
doi：10.1016/0049-3848(89)90256-9
更新日期：1989-11-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.thromres.2009.10.021
更新日期：2010-04-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.thromres.2006.11.007
更新日期：2007-01-01 00:00:00
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journal_title：Thrombosis research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0049-3848(99)00233-9
更新日期：2000-04-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0049-3848(93)90132-8
更新日期：1993-05-01 00:00:00
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更新日期：1993-02-15 00:00:00
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doi：10.1016/j.thromres.2007.03.002
更新日期：2007-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2000-02-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0049-3848(88)90094-1
更新日期：1988-10-15 00:00:00
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journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2010.07.003
更新日期：2010-10-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0049-3848(86)90330-0
更新日期：1986-11-15 00:00:00
abstract：:Plasma from patients with thrombotic thrombocytopenic purpura (TTP) caused the aggregation of washed human platelets, which was inhibited by preincubation with normal plasma. Using salt fractionation, ion exchange chromatography, and preparative agarose gel electrophoresis, we purified a protein from normal plasma whi...
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pub_type： 杂志文章
doi：10.1016/0049-3848(84)90155-5
更新日期：1984-01-01 00:00:00
abstract：:The prevention of venous thromboembolism (VTE) in women with cancer requires special consideration. The presence of cancer results in both non-surgical and surgical patients having increased risk of VTE. This usually leads to a modification of the recommended preventive therapy, affecting the type, dose and length of ...
journal_title：Thrombosis research
pub_type： 杂志文章
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更新日期：2011-02-01 00:00:00
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journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(94)90240-2
更新日期：1994-08-01 00:00:00
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journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(95)00082-3
更新日期：1995-06-15 00:00:00
abstract：BACKGROUND:Cocaine use has been related with the development of accelerated atherosclerosis and with an increased risk of cardiac and cerebrovascular events, such as myocardial infarction, sudden cardiac death, and ischemic stroke. The underlying mechanisms leading to these complications are not fully understood, altho...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2011.04.019
更新日期：2011-10-01 00:00:00
