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Purification and some properties of a protein obtained from normal human plasma which inhibits the platelet aggregation induced by thrombotic thrombocytopenic purpura plasma.

Abstract：

:Plasma from patients with thrombotic thrombocytopenic purpura (TTP) caused the aggregation of washed human platelets, which was inhibited by preincubation with normal plasma. Using salt fractionation, ion exchange chromatography, and preparative agarose gel electrophoresis, we purified a protein from normal plasma which inhibited the platelet aggregation caused by TTP plasma. On SDS polyacrylamide gel, the purified inhibitor gave a single band with a M.W. of 150,000. The antiserum against the purified protein neutralized the activity of the inhibitor and formed an identical precipitin line against normal and TTP plasma.

journal_name

Thromb Res

journal_title

Thrombosis research

authors

Lian EC,Mui PT,Siddiqui FA,Chiu LL

doi

10.1016/0049-3848(84)90155-5

subject

Has Abstract

pub_date

1984-01-01 00:00:00

pages

69-76

issue

1

eissn

0049-3848

issn

1879-2472

pii

0049-3848(84)90155-5

journal_volume

33

pub_type

杂志文章

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