Abstract：
:Washed human platelets were incubated with commercial factor VIII concentrate, or with purified factor VIII coagulant moiety. Platelets were then washed again and lysed by sonication. VIII:Ag and vWf:Ag were measured in the platelet lysate prior to and after incubation of the lysate with phospholipase C (PL-C). Platelet bound VIII:Ag was significantly higher after incubation of washed platelets with factor VIII concentrate than after incubation with buffer. Platelet bound VIII:Ag was further increased when platelets had been incubated with concentrate in the presence of thrombin and collagen. In contrast, only a slight increase in platelet bound vWf:Ag was observed after incubation of platelets with concentrate. When washed platelets had been incubated with factor VIII coagulant moiety, also significantly more platelet bound VIII:Ag was observed than after incubation with buffer. Measurable VIII:Ag, but not vWf:Ag, increased significantly after incubation of the platelet lysate with PL-C. When intact washed platelets had been treated with PL-C prior to the incubation with concentrate, binding of VIII:Ag to platelets was nearly completely abolished. Our data suggest that the factor VIII coagulant moiety binds to phospholipids of the platelet membrane and thereby contributes to the assembly of the factor X activating complex on the platelet surface.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Muntean W,Leschnik B,Haas Jdoi
10.1016/0049-3848(87)90223-4subject
Has Abstractpub_date
1987-02-15 00:00:00pages
345-54issue
4eissn
0049-3848issn
1879-2472journal_volume
45pub_type
杂志文章abstract：:Human antithrombin III was demonstrated to bind plasminogen specifically in a time and concentration-dependent manner. The above binding was also confirmed using ligand western blot assays. The interaction of plasminogen was significantly (>90%) inhibited by lysine, indicating the involvement of kringles in binding an...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(00)00268-1
更新日期：2000-09-15 00:00:00
abstract：INTRODUCTION:Though thrombin-activatable fibrinolysis inhibitor (TAFI) may contribute to hypercoagulability during pregnancy, limited data are available on the role of TAFI in women with recurrent fetal loss. MATERIAL/METHODS:We performed a case-control study aimed at evaluating any possible association between TAFI l...
journal_title：Thrombosis research
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.thromres.2011.09.029
更新日期：2012-08-01 00:00:00
abstract：INTRODUCTION:A higher level of health insurance coverage may be related to better quality of care and outcomes. Whether insurance status is associated with anticoagulation quality and clinical outcomes in patients with venous thromboembolism (VTE) is unknown. METHODS:We studied 819 elderly patients treated with vitami...
journal_title：Thrombosis research
pub_type： 杂志文章,多中心研究
doi：10.1016/j.thromres.2018.11.011
更新日期：2019-01-01 00:00:00
abstract：:Sandwich ELISA systems were developed to measure human von Willebrand factor (vWF). In one system the microtitre plates were coated with goat F(ab')2 to vWF. The F(ab')2 different molecular forms in the soluble phase. VWF antigen bound by the F(ab')2 antibody was subsequently determined by using horse-radish peroxidas...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(86)90047-2
更新日期：1986-07-01 00:00:00
abstract：:Acute promyelocytic leukemia (APL) has become the most curable form of acute myeloid leukemia after the advent of all-trans retinoic acid (ATRA). However, early deaths (ED) mostly due to the disease-associated coagulopathy remain the major cause of treatment failure. In particular, hemorrhagic events account for 40-65...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/S0049-3848(14)50019-9
更新日期：2014-05-01 00:00:00
abstract：:The coumarin derivative AD6 is known to inhibit platelet aggregation and release and it possesses vasodilatory properties on coronary arteries of laboratory animals. Furthermore, the inhibition of the production of TxB2 from endogenous substrates after stimulation of human platelets with collagen has been demonstrated...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(87)90236-2
更新日期：1987-07-01 00:00:00
abstract：:We investigated the age-related changes in blood coagulation, fibrinolysis, and platelet aggregation in male WBN/Kob rats, animals that exhibit spontaneously diabetes mellitus at more than 6 months of age. The rats aged 6 months or more showed significant hyperglycemia, hypoinsulinemia, and hyperlipidemia. As changes ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(00)00196-1
更新日期：2000-06-15 00:00:00
abstract：INTRODUCTION:Sepsis is frequently complicated by disseminated intravascular coagulation (DIC), which promotes multiple organ dysfunctions and significantly increase the mortality of patients with sepsis. How bacteria cause DIC is not fully understood. Outer membrane vesicles (OMVs) are membrane-enclosed microvesicles r...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2019.03.019
更新日期：2019-06-01 00:00:00
abstract：:Platelets from rats fed a diet high in linoleic acid (6%) bound increased amounts of fibrinogen on stimulation with ADP, compared to those from rats fed diets with low (2%) or no linoleic acid. However, this increased fibrinogen binding was associated with a decrease in platelet aggregation induced by ADP. Changes in ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(84)90107-5
更新日期：1984-04-01 00:00:00
abstract：:Eight patients with femoro-popliteal or sural DVT, confirmed by phlebography, were treated with intravenous Desmin (LMW-dermatan sulphate): on the first day, after an initial i.v. injection of 400 mg, all patients received an infusion of 800 mg in 500 ml of saline, during 24 hours; this infusion was repeated in each o...
journal_title：Thrombosis research
pub_type： 临床试验,杂志文章
doi：10.1016/s0049-3848(96)00207-1
更新日期：1996-12-15 00:00:00
abstract：:The activity of tissue plasminogen activator-plasminogen activator inhibitor-1 (t-PA-PAI-1) complex to convert plasminogen to plasmin was examined by using fibrin autography and casein zymography where plasminogen was added or removed for casein layer. Fibrin autography was more sensitive to the activity of t-PA even ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(91)90279-6
更新日期：1991-07-01 00:00:00
abstract：:Anticoagulant therapy for the typical venous thromboembolism patient is straightforward with predictably favorable outcomes. However, for certain patients with venous thromboembolism, there remains uncertainty and controversy about optimal treatment. These controversial areas include venous thromboembolism patients wi...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2006.05.015
更新日期：2007-01-01 00:00:00
abstract：:Cilostazol is a newly developed antiplatelet drug that has been widely applied for clinical use. Its antiplatelet action appears to be mainly related to inhibition of intracellular phosphodiesterase activity. Our study was designed to investigate inhibitory effects of cilostazol on the expression of activation-depende...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(98)00172-8
更新日期：1999-02-01 00:00:00
abstract：:It has been suggested that unstable angina at rest, like acute myocardial infarction, might be associated with a thrombotic process. In order to study the hypothesis that myocardial ischemia during exercise could also be associated with an activation of blood coagulation and/or fibrinolysis, we investigated the presen...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(92)90222-v
更新日期：1992-01-01 00:00:00
abstract：:Fluorescein Dextran (FD) was shown to be transported at increased rates through partially denuded endothelial monolayer. Platelet binding to the partially denuded monolayer lowered transport rates to those comparable with intact endothelium. Inhibition of transport by platelet binding was not affected by the addition ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(92)90191-c
更新日期：1992-05-01 00:00:00
abstract：:Ca2+ influx and efflux in unstimulated platelets and Ca2+ uptake by simultaneously isolated two membrane fractions of platelets from patients with myeloproliferative disorders (MPD) have been investigated. In MPD, Ca2+ influx and efflux across the plasma membrane in unstimulated platelets were in equilibrium at signif...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(89)90372-1
更新日期：1989-01-15 00:00:00
abstract：:It has become customary to regard the various glycerophospholipids as quite similar, and the acyl groups are considered to have little influence on the behaviour of the lipids in membranes or metabolism. Nevertheless, a number of recent observations by the authors and others indicate a high degree of metabolic compart...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/0049-3848(92)90006-v
更新日期：1992-08-01 00:00:00
abstract：:To estimate the effect of dilazep in preventing restenosis after a transluminal angioplasty, we have attempted an animal experiment in which the efficacy of drug was tested on an angioplastic injury superimposed on to atherosclerotic lesions. Using a modification of Block's method, atherosclerotic lesions first were m...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(89)90122-9
更新日期：1989-06-15 00:00:00
abstract：:The potency for the inhibition of 5-hydroxytryptamine (5-HT)-induced human platelet aggregation by ketanserin, pipamperone, spiperone, cyproheptadine and BW 501 in vitro decreased as the reaction pH increased progressively from 7.4 to 8.6, the largest shift (X 1125) in IC50 value (pH 7.4: 4 X 10(-9) M; pH 8.6: 4.5 X 1...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(85)90297-x
更新日期：1985-12-01 00:00:00
abstract：INTRODUCTION:Patients with intracerebral hemorrhage during rivaroxaban treatment have small hematoma and favorable outcomes compared with those with warfarin. We investigated its possible mechanism, focusing on prothrombin fragment 1+2 (F1+2), a marker of thrombin generation. MATERIALS AND METHODS:In 65 patients with ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2016.10.011
更新日期：2016-12-01 00:00:00
abstract：BACKGROUND:Unfractionated heparin (UFH) and low molecular weight heparin constitute fundamental anticoagulants during hemodialysis (HD). We aimed to investigate the effect of UFH and enoxaparin on plasma levels of prothrombin fragment 1+2 (PF 1+2) and thrombin/antithrombin complex (TAT) as markers of intravascular thro...
journal_title：Thrombosis research
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.thromres.2007.12.014
更新日期：2009-02-01 00:00:00
abstract：:A tritium-labelled low molecular weight heparin fragment with an average molecular weight of 4000-6000 (Fragmin), was fractionated into its high and low affinity forms for antithrombin. The fractions obtained were injected into rabbits, and the plasma half-life (t1/2) volume of distribution (Vd), area under the curve ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(87)90345-8
更新日期：1987-10-01 00:00:00
abstract：:We previously reported that the expression of thrombomodulin on the MEG-01, a cell line from human megakaryoblastic leukemia, was increased by agents that increase intracellular cAMP. In this paper we examine the effect of these agents on cultured human umbilical vein endothelial cells (HUVEC) and mouse hemangioma cel...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(91)90107-8
更新日期：1991-02-01 00:00:00
abstract：INTRODUCTION:Early achievement of therapeutic INR leads to shorter hospital stay and lesser cost. Two warfarin initiation nomograms (10 mg nomogram and 5 mg nomogram) are widely used but it is not yet clear which one is better. They have been validated in the West but there are no studies from India. We undertook this ...
journal_title：Thrombosis research
pub_type： 临床试验,信件
doi：10.1016/j.thromres.2018.12.026
更新日期：2019-04-01 00:00:00
abstract：INTRODUCTION:Post-thrombotic syndrome (PTS) is a chronic sequel of deep vein thrombosis (DVT). The clot structure and fibrinolytic potential in PTS is currently unknown. OBJECTIVE:To assess the fibrinolytic potential and clot structure in patients with PTS. MATERIALS AND METHODS:Patients with a history of DVT were in...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2015.11.013
更新日期：2016-01-01 00:00:00
abstract：INTRODUCTION:In the prospective, randomised, double-blind, placebo-controlled Regenerate Vital Myocardium by Vigorous Activation of Bone Marrow Stem Cells (REVIVAL)-2 trial patients with acute myocardial infarction (AMI) and successful mechanical reperfusion received granulocyte-colony stimulating factor (G-CSF, 10 μg/...
journal_title：Thrombosis research
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.thromres.2010.11.004
更新日期：2011-02-01 00:00:00
abstract：BACKGROUND:There is a perception in the orthopaedic and thromboembolism community that the incidence of deep vein thrombosis (DVT) has decreased in patients undergoing total knee arthroplasty (TKA) or total hip arthroplasty (THA). OBJECTIVES:To assess the incidence of DVT with warfarin thromboprophylaxis over time in ...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2008.05.005
更新日期：2008-01-01 00:00:00
abstract：:Clot dissolution using enzymes from the human fibrinolytic system has long been a goal of physicians and scientists. While recent focus has been on activators of the fibrinolytic system, new data support the use of plasmin itself. This supplement reviews the role of plasmin in fibrinolysis and locally administered pla...
journal_title：Thrombosis research
pub_type：
doi：10.1016/j.thromres.2008.06.008
更新日期：2008-01-01 00:00:00
abstract：:All the thrombolytic agents currently in clinical use act as plasminogen activators. In this study evidence is presented that also oxidants of the phagocyte type are of fibrinolytic efficiency in vivo. Activated phagocytes participate in physiologic fibrinolysis. The cells generate plasminogen activators and reactive ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(91)90189-4
更新日期：1991-05-01 00:00:00
abstract：INTRODUCTION:The activated partial thromboplastin time (APTT) is commonly used to monitor unfractionated heparin (UFH) but may not accurately measure the amount of heparin present. The anti-Xa assay is less susceptible to confounding factors and may be a better assay for this purpose. MATERIALS AND METHODS:The validit...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2017.07.006
更新日期：2017-09-01 00:00:00
