Abstract：
INTRODUCTION:Post-thrombotic syndrome (PTS) is a chronic sequel of deep vein thrombosis (DVT). The clot structure and fibrinolytic potential in PTS is currently unknown. OBJECTIVE:To assess the fibrinolytic potential and clot structure in patients with PTS. MATERIALS AND METHODS:Patients with a history of DVT were included in a case-control study: patients with PTS (cases n=30) and without PTS (controls n=30), and 30 apparently healthy individuals (HI) without venous thromboembolism (VTE) or venous insufficiency were enrolled. Fibrinolysis and clot structure were assessed by turbidimetric assays, permeation, and confocal microscopy. Fibrinogen was measured by Clauss and fibrinogen γ' by ELISA. RESULTS:We observed a significant trend of decreasing maximum turbidity from HI (median 0.52 [IQR 0.46-0.62]), to controls (0.49 [IQR 0.41-0.55]), to cases (0.46 [IQR 0.39-0.49]) p=0.020. Fibrinogen was lower in patients (cases and controls) (3.69g/L [IQR 3.31-4.26]) compared to HI (4.17 [IQR 3.69-4.65]) p=0.041. Patients with recurrent VTE had lower maximum turbidity and lower permeation than patients with one episode of VTE; (0.31 [IQR 0.25-0.39] versus 0.38 [IQR 0.34-0.44] p=0.008) and (6.0×10(-9)/cm(2) [IQR 5.1-7.9] versus 7.7×10(-9)/cm(2) [IQR 6.0-10.0] p=0.047) respectively, at equal fibrinogen levels. There were no differences in lysis time, confocal microscopy, or fibrinogen γ'. CONCLUSIONS:Lower maximum turbidity, indicating a tendency towards thinner fibres and denser clots, was found in patients with PTS as well as in patients with recurrent VTE. Fibrinogen levels did not explain these differences in clot structure. The abnormal clot structure may contribute to the increased thrombotic risk profile in patients with PTS.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Bouman AC,McPherson H,Cheung YW,Ten Wolde M,Ten Cate H,Ariëns RAS,Ten Cate-Hoek AJdoi
10.1016/j.thromres.2015.11.013subject
Has Abstractpub_date
2016-01-01 00:00:00pages
85-91eissn
0049-3848issn
1879-2472pii
S0049-3848(15)30189-4journal_volume
137pub_type
杂志文章abstract：BACKGROUND:The recanalization rate in patients with deep venous thrombosis (DVT) of the legs treated with the direct oral anticoagulants (DOAC) is unknown. METHODS:In an Italian cohort, we investigated the rate of residual vein thrombosis (RVT) after three and/or six months in 352 patients with proximal DVT who had be...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2017.03.022
更新日期：2017-05-01 00:00:00
abstract：:Platelets occupy a central role at the interface between thrombosis and inflammation. At sites of vascular damage, adherent platelets physically and functionally interact with circulating leukocytes. Activated platelets release soluble factors into circulation that may have local and systemic effects on blood and vasc...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2010.10.010
更新日期：2011-03-01 00:00:00
abstract：INTRODUCTION:Inter-individual variability in clopidogrel responsiveness is dependent on genetic polymorphisms. We aimed to investigate the impact of 3 genetic polymorphisms involved in clopidogrel metabolism on a strategy of dose-adjustment according to platelet reactivity (PR) monitoring. MATERIAL AND METHODS:[correc...
journal_title：Thrombosis research
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1016/j.thromres.2011.12.038
更新日期：2012-07-01 00:00:00
abstract：:An in vitro model of platelet adhesion, the annular perfusion chamber, was utilized to test the effects of Interleukin 1 on vascular thrombogenicity for platelets. De-endothelialized, everted human umbilical arteries were placed in cell culture media with or without Interleukin 1. The arteries were later perfused with...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(89)90235-1
更新日期：1989-11-15 00:00:00
abstract：INTRODUCTION:Z4A5 is a novel peptide that inhibits platelet aggregation and formation of platelet thrombi, but the mechanism of its anti-platelet effects remains unknown. This study explores the anti-platelet effect and mechanism of Z4A5. METHODS:We investigated the anti-platelet activity of Z4A5 on platelet aggregati...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2012.02.047
更新日期：2012-05-01 00:00:00
abstract：:Lipoprotein(a) [Lp(a)] has been demonstrated to be an independent risk factor for coronary heart disease. However, the precise mechanism by which it contributes to the development of atherosclerosis remains unclear. P-selectin is one of the major adhesion molecules to mediate the interaction between monocytes, platele...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(00)00363-7
更新日期：2000-12-15 00:00:00
abstract：BACKGROUND:The methodology of thromboprophylaxis post minimally invasive esophagectomy (MIE) is unclear. Thus, we compared the efficacy and safety of fondaparinux and nadroparin on the prophylaxis of venous thromboembolism (VTE) after MIE. MATERIALS AND METHODS:We conducted a randomized, double-blind, treatment-contro...
journal_title：Thrombosis research
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.thromres.2018.04.001
更新日期：2018-06-01 00:00:00
abstract：BACKGROUND:Non-vitamin K antagonist oral anticoagulants (NOACs) do not need routine laboratory monitoring but measurement of drug concentration is important in emergency conditions. Specific laboratory tests are not readily available or not implemented in every hospital. Point-of-Care Tests (POCT) may bridge this gap a...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2018.01.044
更新日期：2018-03-01 00:00:00
abstract：:When a purified myeloma protein with the ability to inhibit fibrin polymerization was preincubated with sheep anti-human F(ab')2 antibodies, there was a marked reduction of the ability to prolong the thrombin clotting time, while anti-Fc antibodies had only a slight effect. A monoclonal immunoglobulin which shortens t...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(85)90284-1
更新日期：1985-11-15 00:00:00
abstract：:Anticoagulant therapy for the typical venous thromboembolism patient is straightforward with predictably favorable outcomes. However, for certain patients with venous thromboembolism, there remains uncertainty and controversy about optimal treatment. These controversial areas include venous thromboembolism patients wi...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2006.05.015
更新日期：2007-01-01 00:00:00
abstract：:Disseminated intravascular coagulation (DIC) is a severe complication of septic shock. Polymorphonuclear neutrophils (PMNs) may play a key role in septic shock-induced DIC via the release of neutrophils extracellular traps (NETs). NETs capture invading pathogens, but also act as a pro-coagulant surface at the interfac...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2019.09.036
更新日期：2019-11-01 00:00:00
abstract：:[3H]-Platelet activating factor (Paf-acether, 1-O-octadecyl-2-acetyl-sn-glycero-3-phosphocholine) binds to washed human platelets in a specific, dose-dependent, and saturable manner. Scatchard analysis reveals a high affinity site with a KD value of 0.25 +/- 0.033 nM (245 +/- 30 sites per platelet), and a second low a...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(86)90233-1
更新日期：1986-01-15 00:00:00
abstract：:Practice variation, the differences in clinical management between physicians, is one reason why patient outcomes may differ. Identifying factors that contribute to practice variation in areas of clinical uncertainty or equipoise may have implications for understanding and improving patient care. To discern what facto...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2017.09.026
更新日期：2017-11-01 00:00:00
abstract：:Ten studies of the effect of cryoprecipitate infusion in four patients with von Willebrand's Disease (vWD) are reported. The doses studied (4, 8 and in one case 10 bags of cryoprecipitate) were based on previous reports of correction of the bleeding time (BT) with such doses. A modest shortening of the BT was observed...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(89)90196-5
更新日期：1989-03-01 00:00:00
abstract：INTRODUCTION:Thrombin or tryptase cleavage of protease-activated receptors (PAR) on human coronary artery endothelial cells (HCAEC) results in activation of a membrane-associated, calcium-independent phospholipase A2 (iPLA2) that selectively hydrolyzes plasmalogen phospholipids. Atherosclerotic plaque rupture results i...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2006.11.007
更新日期：2007-01-01 00:00:00
abstract：:The soluble fibrin monomer complexes (SFMC) in 154 obstetric patients with possible disseminated intravascular coagulation (DIC) were evaluated in SDS polyacrylamide gel electrophoresis (PAGE) after precipitation with B-alanine. Other coagulation tests were performed on these patients. The patients were classified int...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(82)90007-x
更新日期：1982-09-15 00:00:00
abstract：:An in vitro test system was developed to examine the effects of red blood cells (RBC) on shear-induced platelet adhesion (SIPAD) and platelet aggregation (SIPAG). Suspensions of human platelets labeled with Mepacrine and suspended in citrated plasma were exposed to single, continuous or repetitive (120-300x) one secon...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(98)00160-1
更新日期：1998-12-15 00:00:00
abstract：:Adenosine is an endogenous antiaggregating substance that influences the platelet responses through specific A-type receptors that activate adenylate cyclase increasing the levels of 3',5'-cyclic adenosine monophosphate (cAMP). In this study, we investigated whether adenosine can also influence the levels of 3',5'-cyc...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(01)00412-1
更新日期：2002-01-01 00:00:00
abstract：:The serotonin transporter (SERT) is found in neuron and platelet membranes. Selective serotonin-reuptake inhibitors (SSRIs) are widely prescribed for severe depression. They may at least partly counteract the effects of serotonin on the vascular biology system, can lower agonists-induced platelet activation, aggregati...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2014.02.028
更新日期：2014-07-01 00:00:00
abstract：INTRODUCTION:There is an exponential rise of thromboembolic risk with age because of co-morbidities, immobility and pharmacotherapy. We aimed to investigate the benefits and risks of heparin prophylaxis in very elderly patients ≥80 years and the type of heparin used in a subgroup analysis of the CERTIFY trial. PATIENT...
journal_title：Thrombosis research
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.thromres.2011.05.002
更新日期：2011-11-01 00:00:00
abstract：:We examined the effects of verapamil on platelet function. Verapamil (0.5 micrograms/ml) in this study inhibited platelet aggregation induced by threshold amounts of ADP, AA, and epinephrine. With higher concentrations of aggregating stimuli, verapamil caused a dose-dependent inhibition of aggregation. Verapamil reduc...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(83)90181-0
更新日期：1983-06-01 00:00:00
abstract：BACKGROUND:Variability in platelet response to clopidogrel and its clinical relevance have been well described. However, the underlying mechanisms remain unclear. Recently, the T744C polymorphism of the P2Y12 receptor gene has been associated with enhanced platelet aggregation in healthy volunteers, suggesting a possib...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2007.01.012
更新日期：2007-01-01 00:00:00
abstract：:The Coa-set Fibrin Monomer test (CFM-test) is a quantitative method for determination of soluble fibrin in plasma. The fibrin standard of the CFM-test is produced by bathroxobin conversion of purified fibrinogen to fibrin. Plasma treated with minute amounts of thrombin may be considered as a more physiological way of ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(93)90139-f
更新日期：1993-11-15 00:00:00
abstract：:A simple and discriminating assay for the determination of the fast-acting plasminogen activator inhibitor (PAI) activity in human plasma is described. The method is based on the inhibition of purified tissue plasminogen activator (tPA) by plasma diluted with a PAI-depleted plasma and the subsequent measurement of res...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(89)90250-8
更新日期：1989-11-01 00:00:00
abstract：:Protein C has been measured by three different assays (antigenic, amidolytic and chronometric) in 27 end-stage renal insufficient patients before and after hemodialysis. Protein C levels have been compared with other coagulation inhibitors (antithrombin III, protein S) and fibrinolytic parameters. Baseline anticoagula...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(93)90055-s
更新日期：1993-03-15 00:00:00
abstract：:Fibrin film is prepared by compaction in one dimension of a fibrin clot (pH 6.3, ionic strength 0.15, fibrin concentration about 0.5%) by expulsion of fluid to reach a fibrin concentration of about 15%. Strips of film, equilibrated in the same buffer with very slowly increasing temperature, shrink in length in a narro...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(88)90020-5
更新日期：1988-12-01 00:00:00
abstract：BACKGROUND:There is a substantial local release of tissue-type plasminogen activator (t-PA) in the splanchnic vascular bed, and this release is increased at high sympathetic tone. Coronary t-PA release is also significant, and this release increases during cardiac nerve stimulation and during reperfusion after 10 min o...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2009.10.004
更新日期：2010-03-01 00:00:00
abstract：INTRODUCTION:Hip fracture is common among the elderly and is associated with increased morbidity and mortality, particularly when surgery is delayed. Direct oral anticoagulants (DOACs) use might increase bleeding and postpone hip repair surgery. We aimed to assess the association between preoperative DOACs use and adve...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2020.03.002
更新日期：2020-05-01 00:00:00
abstract：:Factor IX Nagoya 3 (IX Nagoya 3) is a natural mutant of factor IX recognized in a patient with moderately severe hemophilia B. The patient had 0.60 units/ml of factor IX antigen and 2-5% of clotting activity. IX Nagoya 3 was purified from the patient's plasma by immunoaffinity chromatography with an anti-factor IX mon...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(90)90109-p
更新日期：1990-11-15 00:00:00
abstract：:A platelet storage pool deficiency (SPD) is present in platelets from cattle with the Chediak-Higashi syndrome (CHS). The most plausible hypothesis for the SPD is that dense granule precursors are simply not formed in CHS megakaryocytes. There is, however, evidence that some recently acquired 5-hydroxytryptamine (5HT)...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(90)90096-u
更新日期：1990-05-01 00:00:00
