Abstract:
INTRODUCTION:Z4A5 is a novel peptide that inhibits platelet aggregation and formation of platelet thrombi, but the mechanism of its anti-platelet effects remains unknown. This study explores the anti-platelet effect and mechanism of Z4A5. METHODS:We investigated the anti-platelet activity of Z4A5 on platelet aggregation induced by adenosine diphosphate (ADP), arachidonic acid (AA) and thrombin (TH) in human platelet-rich plasma (PRP). Fibrinogen and PAC-1 binding to glycoproteinIIb/IIIa (GPIIb/IIIa) were measured by flow cytometry. In addition, we investigated the integrin specificity of Z4A5 in attachment and detachment assays using human umbilical vein endothelial cells (HUVEC) and assessed the relative cell number using the MTT assay. RESULTS:In vitro, Z4A5 inhibited ADP-, AA- and TH-induced human platelet aggregation with IC(50) values of 0.46 ± 0.05 μM (n = 10), 0.23 ± 0.0 5 μM (n = 10) and 0.21 ± 0.02 μM (n = 10), respectively. Z4A5 inhibited fibrinogen, and PAC-1 bound to platelet GPIIb/IIIa with IC(50) values of 0.48 ± 0.07 μM (n = 8) and 0.63 ± 0.12 μM (n = 6), respectively. Z4A5 failed to inhibit α(V)β(3) integrin-mediated HUVEC attachment to vitronectin and did not cause any significant detachment of HUVEC monolayer when compared with the controls. CONCLUSIONS:Z4A5 is a potential anti-platelet drug that inhibits fibrinogen binding to GPIIb/IIIa, but does not affect the structurally similar integrin α(V)β(3).
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Li YX,Sun Q,Zhang H,Ren ST,Liao YR,Wang Y,Shen XL,Wang Bdoi
10.1016/j.thromres.2012.02.047subject
Has Abstractpub_date
2012-05-01 00:00:00pages
e217-22issue
5eissn
0049-3848issn
1879-2472pii
S0049-3848(12)00095-3journal_volume
129pub_type
杂志文章abstract:INTRODUCTION:Peden et al. have revealed a significant association between four new risk loci and coronary heart disease (CHD) in Europeans and South Asians. The goal of this study is to evaluate the contribution of these genetic loci to CHD risk in Han Chinese. METHODS:We recruited 161 CHD patients and 112 controls pr...
journal_title:Thrombosis research
pub_type: 杂志文章,meta分析
doi:10.1016/j.thromres.2012.05.023
更新日期:2012-10-01 00:00:00
abstract::Full-length Tissue Factor (flTF) - the obligatory co-factor for the serine protease (factor) VII/VIIa - serves as the initiator of blood coagulation. The flTF/VIIa complex triggers a sequence of proteolytic events that lead to the formation of a hemostatic plug. Aside from hemostatic maintenance, flTF can contribute t...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/j.thromres.2012.02.027
更新日期:2012-05-01 00:00:00
abstract::Diabetes mellitus (DM) is associated with platelet and leukocyte dysfunction. Previous observations with regard to insulin effects on platelet and leukocyte function are less than consistent. We thus investigated the effects of insulin on platelets and leukocytes, as well as on platelet-leukocyte interactions in whole...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(02)00306-7
更新日期:2002-09-01 00:00:00
abstract:INTRODUCTION:Diabetes mellitus is characterised by hyperglycaemia that plays an important role in the pathogenesis of diabetic complications including accumulation of methylglyoxal (MG), a highly reactive alpha-dicarbonyl metabolite of glucose degradation pathways and increased generation of advanced glycation end prod...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2007.03.002
更新日期:2007-01-01 00:00:00
abstract:INTRODUCTION:Viscoelastometry enables rapid evaluation of coagulopathy in settings such as cardiac surgery but may be influenced by red cell concentration. METHODS:In order to study the effects of supra-physiological red cell concentrations on viscoelastometry, we compared ROTEM® viscoelastometry and plasma coagulatio...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2013.07.006
更新日期:2013-08-01 00:00:00
abstract:BACKGROUND:Thrombosis and complement activation are pathogenic features of antiphospholipid syndrome (APS). Their molecular link is Plasma carboxypeptidase-B, also known as thrombin activatable fibrinolysis inhibitor (TAFIa), which plays a dual role: anti-fibrinolytic, by cleaving carboxyl-terminal lysine residues from...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2017.06.028
更新日期:2017-10-01 00:00:00
abstract::A platelet storage pool deficiency (SPD) is present in platelets from cattle with the Chediak-Higashi syndrome (CHS). The most plausible hypothesis for the SPD is that dense granule precursors are simply not formed in CHS megakaryocytes. There is, however, evidence that some recently acquired 5-hydroxytryptamine (5HT)...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(90)90096-u
更新日期:1990-05-01 00:00:00
abstract:INTRODUCTION:The recombinant fusion protein linking coagulation factor IX with albumin (rIX-FP) is undergoing clinical trials for prophylaxis and on-demand treatment of haemophilia B patients. The aim of this study was to investigate the pharmacokinetics, whole-body and knee joint distribution of rIX-FP following intra...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2014.02.010
更新日期:2014-05-01 00:00:00
abstract::Porcine and human umbilical vein and adult blood vessels were studied for the presence and synthesis of factor VIII related antigen (VIIIR:Ag) and fibronectin (Fn) by immunofluorescence histology and immunoautoradiography. Investigation of human tissue confirmed the widespread distribution of VIIIR:Ag on the endotheli...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(83)90042-7
更新日期:1983-02-01 00:00:00
abstract::Activated platelets secrete different chemokines, among others CCL5, thereby triggering inflammatory cell recruitment into the vessel wall. Here, we investigated how CCL5 deficiency influences vascular remodeling processes. Experiments were performed in apolipoprotein E and CCL5 double deficient (ApoE(-/-)×CCL5(-/-)) ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2016.06.013
更新日期:2016-08-01 00:00:00
abstract::Von Willebrand Factor is a multimer produced by endothelial cells and megakaryocytes, being stored in intracellular organelles, such as the Weibel-Palade bodies and alpha-granules in endothelial cells and platelets, respectively. This molecule acts as a carrier protein for factor VIIIc, involved in the intrinsic pathw...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(99)00122-x
更新日期:2000-01-15 00:00:00
abstract:BACKGROUND:Coronary collateral circulation (CCC) plays an important role in protecting myocardium from ischemic damage. The studies on factors which impact on CCC might be of great clinical interest. The aim of the present study was to evaluate endothelin-1 (ET-1) as a potential predictor for poor or good CCC in patien...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2016.04.007
更新日期:2016-06-01 00:00:00
abstract::A blinded randomized multicenter trial of two non-activated prothrombin complex concentrates was carried out to determine the clinical effectivity in the treatment of acute hemarthrosis in hemophiliac patients with inhibitors. The one product was prepared via DEAE Sephadex chromatography, while the second was fraction...
journal_title:Thrombosis research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/0049-3848(86)90115-5
更新日期:1986-06-15 00:00:00
abstract::We investigated the effects of Defibrotide (D), a natural polydeoxyribonucleotide, on acute myocardial ischemia (AMI) in anesthetized cats. A permanent ligature was placed around the left anterior descending coronary artery (LAD) 12-14 mm from its origin. Ventricular fibrillation and death were exceptional and when th...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(85)90008-8
更新日期:1985-04-01 00:00:00
abstract:INTRODUCTION:The contribution of platelet activation to the pathogenesis of sickle cell disease (SCD) remains uncertain. We evaluated the safety and efficacy of eptifibatide, a synthetic peptide inhibitor of the αIIbβ3 receptor, in SCD patients during acute painful episodes. MATERIALS AND METHODS:In this single site, ...
journal_title:Thrombosis research
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.thromres.2013.08.002
更新日期:2013-09-01 00:00:00
abstract::The purpose of this study was to investigate the effects of tissue factor (thromboplastin), the initiating factor of the extrinsic clotting system, on angiogenesis in vivo and in vitro. In vivo angiogenesis was examined using a diffusion chamber assay in rats. After a week of implantation of the diffusion chambers con...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(99)00101-2
更新日期:1999-11-01 00:00:00
abstract:OBJECTIVES:Dabigatran etexilate is widely used for stroke prevention in the patients with atrial fibrillation. The anticoagulation activity of dabigatran is not necessary monitored in routine clinical practice. We aimed to study the effect of dabigatran on thrombin generation (TG) and coagulation assays in rabbit and h...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2018.03.012
更新日期:2018-05-01 00:00:00
abstract::Heparanase is an endo-beta-D-glucuronidase capable of cleaving heparan sulfate (HS) side chains of heparan sulfate proteoglycans (HSPG) on cell surfaces and the extracellular matrix, activity that is strongly implicated in tumor metastasis and angiogenesis. Evidence was provided that heparanase over-expression in canc...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/S0049-3848(10)70018-9
更新日期:2010-04-01 00:00:00
abstract:INTRODUCTION:Thrombin, a serine protease, plays an important role in such actions as coagulation, cell proliferation and inflammation. It has been sporadically reported that endothelial cells, when stimulated by thrombin via protease-activated receptors (PAR), express various mediators and proteins including cytokines,...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2005.11.008
更新日期:2006-01-01 00:00:00
abstract::The thrombin inhibitory role of antithrombin III (ATIII) and heparin cofactor II (HCII) was studied in vitro using intact and injured rabbit aortae. When intact vessels were loaded with thrombin and then exposed to either heat defibrinogenated human plasma (HDHP) or ATIII the same degree of thrombin inhibition was ach...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(91)90014-n
更新日期:1991-06-01 00:00:00
abstract:INTRODUCTION:There is an exponential rise of thromboembolic risk with age because of co-morbidities, immobility and pharmacotherapy. We aimed to investigate the benefits and risks of heparin prophylaxis in very elderly patients ≥80 years and the type of heparin used in a subgroup analysis of the CERTIFY trial. PATIENT...
journal_title:Thrombosis research
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.thromres.2011.05.002
更新日期:2011-11-01 00:00:00
abstract:INTRODUCTION:The activated partial thromboplastin time (APTT) is commonly used to monitor unfractionated heparin (UFH) but may not accurately measure the amount of heparin present. The anti-Xa assay is less susceptible to confounding factors and may be a better assay for this purpose. MATERIALS AND METHODS:The validit...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2017.07.006
更新日期:2017-09-01 00:00:00
abstract:INTRODUCTION:Endothelium-derived nitric oxide (NO) is synthesized from l-arginine by endothelial nitric oxide synthase (eNOS) encoded by the eNOS3 gene on chromosome 7. The effects of the eNOS polymorphisms with the risk of coronary artery disease are conflicting. In this study, we investigated the association of the e...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2006.06.005
更新日期:2007-01-01 00:00:00
abstract::The effect of the vasopressin analogue DDAVP on Factor VIII in plasma has been extensively studied in humans. To examine the effect of new and potentially better analogues a suitable animal model has to be established. In the present study trained Beagle dogs were injected with DDAVP and the time course of the Factor ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(87)90363-x
更新日期:1987-09-01 00:00:00
abstract:BACKGROUND:Cardiovascular mortality is especially low in southwest France (the French Paradox). In previous experimental studies, we found that alcohol-free extracts of armagnac could inhibit human platelet function in vitro and experimental thrombosis in vivo. To test the possible relevance of these findings, we teste...
journal_title:Thrombosis research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/j.thromres.2004.07.006
更新日期:2005-01-01 00:00:00
abstract::Stimulation of rat platelets with U46619 induced Ca2+ mobilization and platelet shape change, but aggregation and secretion were induced when platelets were stimulated with U46619 plus phorbol 12-myristate 13-acetate (PMA). However, stimulation of rabbit platelets with U46619 induced all the three platelet responses. ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(89)90171-0
更新日期:1989-10-15 00:00:00
abstract::The effects of extracellular sodium on platelet aggregation and calcium mobilization in platelets stimulated with either rattlesnake (Crotalus atrox) lectin (RSL) or alpha-thrombin were compared. The absence of extracellular sodium had no effect on platelet aggregation or calcium mobilization in response to all levels...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(91)90008-k
更新日期:1991-11-01 00:00:00
abstract:BACKGROUND:Although patients with idiopathic VTE are at higher than normal risk of asymptomatic atherosclerosis and of cardiovascular events, the impact of cardiovascular risk factors on VTE is poorly understood. OBJECTIVE:To assess the prevalence of the metabolic syndrome and of its components in patients with early-...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2010.12.005
更新日期:2011-03-01 00:00:00
abstract::Three collagenases were purified from the culture medium of human skin fibroblasts using ammonium sulfate fractionation, ion-exchange chromatography and gel filtration. The cationic collagenase had a molecular weight of 42,000; two anionic collagenases had molecular weights of 63,000 and 115,000. Preincubation of the ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(84)90211-1
更新日期:1984-07-15 00:00:00
abstract::The standard modality of administration of rFVIIa to patients with FVIII and FIX inhibitors is the intermittent infusion every 2 to 6 hours. No untoward local or systemic effects have been reported; laboratory data of activation of coagulation were reported in the presence of coexistent problems (sepsis, septic shock)...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(00)00220-6
更新日期:2000-07-01 00:00:00