Abstract：
:The present investigation has used an aggregation-disaggregation-reaggregation model to determine if exposure to potent aggregating agents causing a refractory state in deaggregated platelets results in down-regulation and clearance of GPIb/IX, the receptor for von willebrand factor. Thrombin, the thrombin receptor activating peptide (TRAP) and the thromboxane A2 mimic, U46619, caused irreversible aggregation and secretion when stirred on an aggregometer with plateletrich plasma (PRP). Addition of prostaglandin E1 (PGE1) after the plateau of maximum aggregation was reached caused rapid dissociation of platelet aggregates. Dissociated platelets were refractory to a second exposure to the primary stimulus or to other aggregating agents. Exposure of the refractory cells to epinephrine before the primary agent restored sensitivity resulting in a second wave of irreversible aggregation. Deaggregated, refractory platelets, however, retained their sensitivity to ristocetin. Amounts of the antibiotic causing agglutination of resting PRP also caused agglutination of disaggregated, refractory platelets. Addition of epinephrine to samples of refractory platelets less sensitive to low concentrations of the antibiotic restored their capacity to undergo irreversible ristocetin-induced agglutination. Analysis of the frequency of gold particles associated with monoclonal antibodies directed against GPIb/IX on control platelets and disaggregated, refractory platelets showed no significant difference in the number of receptors. The findings indicate that significant numbers of GPIb/IX receptors remain on platelet surfaces following exposure to potent aggregating agents.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
White JG,Rao GHdoi
10.1016/s0049-3848(96)00185-5subject
Has Abstractpub_date
1996-11-15 00:00:00pages
253-66issue
4eissn
0049-3848issn
1879-2472pii
S0049-3848(96)00185-5journal_volume
84pub_type
杂志文章abstract：INTRODUCTION:Association between ADAMTS13 levels and cardiovascular events has been described recently. However, no genetic study of ADAMTS13 in coronary patients has been described. MATERIALS AND METHODS:Based on related populations frequencies and functional studies, we tested three ADAMTS13 polymorphisms: C1342G (Q...
journal_title：Thrombosis research
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.thromres.2009.03.008
更新日期：2010-01-01 00:00:00
abstract：INTRODUCTION:In patients with new symptoms in a leg previously affected by deep vein thrombosis (DVT), the presence of thrombus on ultrasound cannot be assumed to be due to recurrent thrombosis. Several parameters have been suggested to differentiate between acute and chronic thrombus on ultrasound, including measureme...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2005.02.011
更新日期：2006-01-01 00:00:00
abstract：BACKGROUND:Clinical parameters, biomarkers and imaging-based risk stratification are widely accepted in pulmonary embolism(PE). The present study has investigated the prognostic role of simplified Pulmonary Embolism Severity Index (sPESI) score and the European Society of Cardiology (ESC) model. METHODS:This prospecti...
journal_title：Thrombosis research
pub_type： 杂志文章,多中心研究
doi：10.1016/j.thromres.2014.02.032
更新日期：2014-06-01 00:00:00
abstract：BACKGROUND:Although risk of hospital-associated venous thromboembolism (HA-VTE) differs between critically and non-critically ill children, studies to date have not led to distinct, pragmatic risk scores. OBJECTIVE:To determine risk factors for HA-VTE in critically ill children not undergoing cardiothoracic surgery, i...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2015.04.036
更新日期：2015-10-01 00:00:00
abstract：:Although the possible occurrence of systemic fibrinogenolysis has been suggested in patients with metastasising prostatic cancer (MPC), direct evidence is lacking. We report on a patient with MPC whose laboratory data were consistent with hyperfibrinolysis: marked decrease of alpha 2-antiplasmin (AP) level (less than ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(92)90047-e
更新日期：1992-06-15 00:00:00
abstract：:The association between blood coagulation and cancer growth and metastatic dissemination is not yet completely understood. In this study we demonstrate that thrombin is capable of enhancing tumor cell adhesive properties and thereby increases tumor cell metastatic potential. Following exposure to alpha-thrombin, Walke...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(92)90081-k
更新日期：1992-11-01 00:00:00
abstract：:We examined the effects of verapamil on platelet function. Verapamil (0.5 micrograms/ml) in this study inhibited platelet aggregation induced by threshold amounts of ADP, AA, and epinephrine. With higher concentrations of aggregating stimuli, verapamil caused a dose-dependent inhibition of aggregation. Verapamil reduc...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(83)90181-0
更新日期：1983-06-01 00:00:00
abstract：:The ability of the COA-SET Fibrin monomer (COA-SET FM) test to detect soluble fibrin was evaluated by comparing the results of the COA-SET FM test with fibrinopeptide A (FPA) determinations following thrombin incubation of plasma or whole blood. In addition, two semiquantitative tests (erythrocytes-agglutination test ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(90)90066-l
更新日期：1990-02-15 00:00:00
abstract：INTRODUCTION:Although recurrent venous thromboembolism is a known risk factor for chronic thromboembolic pulmonary hypertension (CTEPH), the prevalence of CTEPH after recurrent pulmonary embolism (PE) is not clear. MATERIALS AND METHODS:A cohort screening study was performed to clarify the prevalence of echocardiograp...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2011.07.045
更新日期：2011-12-01 00:00:00
abstract：:Cancer-associated arterial thrombotic events (ATEs) are increasingly recognized in specific malignancies and in association with the expanding armamentarium of novel chemotherapeutic agents. The improved cancer survival led to cardiovascular complications becoming clinically relevant many years after cancer diagnosis....
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2018.01.003
更新日期：2018-04-01 00:00:00
abstract：BACKGROUND:Predictors of venous thromboembolism (VTE) after trauma are uncertain. OBJECTIVE:To identify independent predictors of VTE after acute trauma. METHODS:Using Rochester Epidemiology Project (REP) resources, we identified all Olmsted County, MN residents with objectively-diagnosed incident VTE within 92days a...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2016.03.026
更新日期：2016-08-01 00:00:00
abstract：BACKGROUND:It has been previously shown that platelets of patients with diabetes are more reactive and less responsive to anti-platelet drugs compared with platelets from subjects without diabetes. Studies examining the effect of glycemic control on platelet reactivity have yielded conflicting data. Thus, in this study...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2014.05.010
更新日期：2014-07-01 00:00:00
abstract：:Incubation of recombinant human tissue factor apoprotein (Apo-TF) with human plasma decreased the recalcified clotting time of this plasma in a time-and dose-dependent manner suggesting relipidation of the Apo-TF by plasma lipoproteins. Incubation of Apo-TF with purified preparations of human very low density, low den...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(90)90182-c
更新日期：1990-11-01 00:00:00
abstract：:An abnormal prothrombin has been detected in a 26-year-old female, who had no history of excessive bleeding. Prothrombin activity was approximately 10% when measured using either the classical one-stage assay or the assay with Echis carinatus venom, whereas prothrombin antigen level was normal. In keeping with current...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(91)90373-5
更新日期：1991-06-15 00:00:00
abstract：:Two types of LMW heparin were prepared by gel filtration of standard heparin (LMW fraction) and by degradation of heparin by nitrous acid (LMW fragment), respectively. The effects on factor Xa inhibition (XaI), APTT, platelet aggregation and AT III level of these preparations were studied after subcutaneous administra...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(83)90091-9
更新日期：1983-11-15 00:00:00
abstract：:Cathepsin A-like enzyme released from human platelets by thrombin hydrolyzed at the highest rate Cbz-Phe-Ala, Cbz-Phe-Met and Cbz-Phe-Leu, did not require activators and was inhibited by DFP, DCI and mercurial compounds (mersalyl acid, PCMS, PCMB and HgCl2). The optimum activity of secreted enzyme was at pH 5.0-6.0. C...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(97)00084-4
更新日期：1997-06-01 00:00:00
abstract：:A quantitative assessment was made of the distribution of arachidonic acid (AA), eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA), and docosahexaenoic acid (DHA) among the individual sub-classes (diacyl, alkylacyl, alkenylacyl) of the various platelet phospholipids of human subjects consuming a fish oil concen...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(88)90181-8
更新日期：1988-04-01 00:00:00
abstract：INTRODUCTION:The outstanding importance of (soluble) CD40L to cardiovascular disease (CVD) is becoming increasingly apparent as CD40L is an important mediator of thrombotic and inflammatory processes. Platelets are the main source for CD40 ligand, linking platelet stimulatory events to inflammation and adverse adaptive...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2012.04.017
更新日期：2012-09-01 00:00:00
abstract：:Eicosapentaenoic acid (EPA) has been reported to have a potent anti-aggregatory activity and to be efficiently metabolized by 12-lipoxygenase, not by cyclooxygenase in platelets. In vitro effect of 12-lipoxygenase metabolites of EPA on platelet function was studied and compared with those of arachidonic acid (AA). The...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(86)90248-3
更新日期：1986-02-01 00:00:00
abstract：:Thrombocytopenia is a common finding in cancer patients and can have different and/or multifactorial pathogenesis. While in solid tumors it occurs often as a consequence of chemotherapy treatment, it is frequently observed at diagnosis in patients with hematological malignancies being aggravated to a potentially life-...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2018.02.001
更新日期：2018-04-01 00:00:00
abstract：INTRODUCTION:Blood flow induced shear stress plays an important role in platelet and endothelial cell functions. The goal of this study was to investigate the effect of physiologically relevant dynamic shear stress on platelet and endothelial cells. MATERIALS AND METHODS:Pulsatile shear stress waveforms mimicking the ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2010.11.021
更新日期：2011-03-01 00:00:00
abstract：:Thrombin was acetylated by treatment with acetic anhydride, and the potency for protein C activation and the fibrinogen clotting activity of the resultant acetylthrombin were investigated in vitro and in vivo. The acetylthrombin retained an amidolytic activity to the synthetic substrate, S-2238, and reduced both of th...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(90)90053-f
更新日期：1990-08-15 00:00:00
abstract：BACKGROUND:Bypassing agents are the first line therapy in patients with acquired haemophilia A (AHA). Activated prothrombin complex concentrate (aPCC) proved to be effective as initial treatment, but 20% of patients (pts) had relapses. aPCC as short-term prophylaxis to reduce subsequent bleeds is still not clear. AIM:...
journal_title：Thrombosis research
pub_type： 信件
doi：10.1016/j.thromres.2018.12.006
更新日期：2019-02-01 00:00:00
abstract：:The concentrations of tissue plasminogen activator (t-PA) and urokinase (UK) were measured in the plasma of patients with benign or malignant ovarian and uterine tumors. Plasma levels of t-PA were higher in patients with malignant ovarian and uterine tumors than in patients with benign tumors. Plasma UK levels were, h...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(90)90207-s
更新日期：1990-05-15 00:00:00
abstract：:Plasma homocysteine levels result from the effect of genetic and environmental factors. We investigated the hypothesis of familial association between folates, methylenetetrahydrofolate reductase (MTHFR) and hyperhomocysteinemia with acute events, studying three families through pedigree analysis and log-linear graphi...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(01)00408-x
更新日期：2002-01-15 00:00:00
abstract：:We have studied the synthesis and subcellular distribution of the glycosylated membrane protein tissue factor (TF) in human blood monocytes and the inducible monocyte like cell line, HL-60. Following induction with endotoxin or the phorbol ester, TPA, tissue factor specific activity was measured in intact cells, sonic...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(87)90405-1
更新日期：1987-11-15 00:00:00
abstract：BACKGROUND:Troponin I (cTnI), myoglobin, heart-type fatty acid binding protein (H-FABP), and natriuretic peptides (BNP, NTproBNP) were all reported to be elevated in patients with pulmonary embolism (PE). METHODS:To assess the correlation between the aforementioned markers and helical computed tomography (hCT) right v...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2007.07.003
更新日期：2008-01-01 00:00:00
abstract：:Fluorescein Dextran (FD) was shown to be transported at increased rates through partially denuded endothelial monolayer. Platelet binding to the partially denuded monolayer lowered transport rates to those comparable with intact endothelium. Inhibition of transport by platelet binding was not affected by the addition ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(92)90191-c
更新日期：1992-05-01 00:00:00
abstract：:The changes in the fibrinolytic system of rats induced by repeated injections of tissue plasminogen activator (TPA) have been studied. The euglobulin fraction fibrinolytic activity increases 10 min after the first injection of TPA. Repeated injections of TPA for one or more days do not stimulate the fibrinolytic activ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(82)90075-5
更新日期：1982-08-01 00:00:00
abstract：:The study of the interaction between activated protein C (APC) and non-plasmatic inhibitors allowed us to demonstrate that aprotinin is a potent competitive inhibitor of APC with a Ki of 1.35 mumol/L. It was possible to adsorb immunopurified protein C (PC) activated by venom activator to insolubilized aprotinin and to...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(90)90268-h
更新日期：1990-07-01 00:00:00
