Abstract:
INTRODUCTION:Hydrogen sulfide (H2S) known as a gasotransmitter is increasingly recognized for its anti-adhesive, anti-inflammatory and vasoactive properties. Due to these properties, we analysed anti-thrombotic effects of H2S and the participation of the nitric oxide synthase (NOS)-pathway. MATERIALS AND METHODS:In individual venules of the ear of hairless SKH1-hr mice, thrombus formation was induced using a phototoxic light/dye-injury model and intravital fluorescence microscopy. Animals were treated intravenously with the H2S donor Na2S or NaCl as control. In a second setting, the NOS inhibitor L-NAME was applied intraperitoneally as a bolus 12h prior to Na2S treatment and thrombus induction. Blood and ear tissue were sampled after microscopy for assessment of plasma concentrations of soluble (s)P-selectin, sE-selectin, sVCAM-1 and sICAM-1 and expression of endothelial (e)NOS and inducible (i)NOS, respectively. RESULTS:When mice were treated with Na2S, venular thrombus formation was significantly delayed versus that in animals of the NaCl-treated control group. While plasma levels of pro-thrombotic adhesion molecules were not affected by Na2S, immunohistochemistry of the vessel walls showed a significant up-regulation of eNOS and iNOS expression within the Na2S-treated group. The delay of thrombus formation in the Na2S-group was partly but significantly reverted by application of L-NAME. CONCLUSIONS:The anti-thrombotic efficacy of H2S involves the NOS-pathway and may be of preventive and therapeutic value for clinical disorders with increased risk of thrombotic events.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Kram L,Grambow E,Mueller-Graf F,Sorg H,Vollmar Bdoi
10.1016/j.thromres.2013.07.010subject
Has Abstractpub_date
2013-08-01 00:00:00pages
e112-7issue
2eissn
0049-3848issn
1879-2472pii
S0049-3848(13)00288-0journal_volume
132pub_type
杂志文章abstract::Heparin is an effective, relatively safe, inexpensive parenteral antithrombotic agent widely used in the prevention and treatment of thromboembolic disorders, but it has several limitations such as the marked intra- and inter-patient variability in its anticoagulant response, its poor bioavailability at low doses and ...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
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abstract:BACKGROUND:Influencing factors for clopidogrel-mediated platelet inhibition have only been evaluated by one or two different test systems in the same population so far. Since previous studies revealed poor correlations between the various platelet function tests, the identification of influencing variables for clopidog...
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pub_type: 临床试验,杂志文章
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journal_title:Thrombosis research
pub_type: 杂志文章,meta分析,评审
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pub_type: 杂志文章,评审
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journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2017.09.028
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pub_type: 临床试验,杂志文章,多中心研究
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journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(94)90050-7
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pub_type: 杂志文章
doi:10.1016/j.thromres.2015.05.007
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abstract:BACKGROUND:Critical care patients are prone to venous thromboembolism (VTE) and, thus, pharmacological thromboprophylaxis is generally advised. Low-molecular weight heparins (LMWHs) have become the drug of choice in ICU patients, since their predictable and reproducible dose response. Monitoring their pharmacological e...
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pub_type: 杂志文章,meta分析
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pub_type: 杂志文章
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pub_type: 杂志文章,评审
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journal_title:Thrombosis research
pub_type: 临床试验,杂志文章,随机对照试验
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journal_title:Thrombosis research
pub_type: 杂志文章
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journal_title:Thrombosis research
pub_type: 杂志文章,meta分析
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(98)00173-x
更新日期:1999-02-15 00:00:00
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pub_type: 临床试验,杂志文章
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pub_type: 杂志文章
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