Abstract:
INTRODUCTION:Different Klebsiella pneumoniae strains carry different virulence factors and antibiotic resistance and may cause thrombocytopenia. This study aimed to investigate the effects of different infections caused by K. pneumoniae on platelets. METHODS:Two hypermucoviscous K. pneumoniae strains and two classic strains were collected from clinical blood culture, and in both groups, there was a carbapenem-resistant strain and a carbapenem-sensitive strain. Mouse infection models were constructed by intraperitoneally injecting different strains, and mice injected with phosphate-buffered saline served as a control. Count, aggregation rate and apoptosis proportion of platelets within 12 h were examined. CD41 expression was measured in bone marrow cells to determine the maturation of megakaryocytes. The concentrations of lipopolysaccharides and related signaling molecules were also measured. RESULTS:The platelet aggregation rate was much significantly higher in the two hypermucoviscous groups, while it showed no difference in the classic groups compared to the control group. All infections induced apoptosis of platelets, among which the highest apoptosis proportions were observed in infections caused by the hypermucoviscous carbapenem-sensitive strain. In both hypermucoviscous groups the CD41 mean fluorescence intensity was much lower than that in the control group, indicating that the maturation of megakaryocytes in the hypermucoviscous groups was significantly inhibited. Lipopolysaccharides were significantly higher and TLR4/Myd88 and JNK/MAPK pathways were strongly activated in hypermucoviscous groups. CONCLUSIONS:The results indicate that hypermucoviscous K. pneumoniae can reduce platelet count by several pathways. Although antibiotic resistance is rapidly emerging worldwide, it has little influence on the decrease in platelets.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Wang Z,Ren J,Liu Q,Li J,Wu X,Wang W,Wu J,Wang G,Li Jdoi
10.1016/j.thromres.2018.09.053subject
Has Abstractpub_date
2018-11-01 00:00:00pages
45-54eissn
0049-3848issn
1879-2472pii
S0049-3848(18)30537-1journal_volume
171pub_type
杂志文章abstract:BACKGROUND:In the rat liver, growth hormone (GH) affects the synthesis of vitamin-K-dependent factors, including Protein C (prot.C) and protein S (prot.S), two natural anticoagulants that prevent hypercoagulable states. Adults with GH deficiency (GHD) are at risk of thrombotic events. High circulating levels of PAI-1 a...
journal_title:Thrombosis research
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1016/j.thromres.2010.08.028
更新日期:2010-12-01 00:00:00
abstract:INTRODUCTION:Pseudoprolongation of activated partial thromboplastin time (APTT) is a serious problem in anticoagulation therapy for patients with high hematocrit, such as cyanotic congenital heart diseases. APTT pseudoprolongation occurs when APTT assay is performed using routinely used vacuum sampling tubes containing...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2007.08.004
更新日期:2008-01-01 00:00:00
abstract::Diagnosis and risk stratification of patients with acute coronary syndromes remain extremely important in order to avoid unnecessary hospitalizations on the one hand, and to improve prognosis of these patients on the other. For diagnosis of acute coronary syndromes, an ECG should be obtained at rest, troponin T and I ...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/s0049-3848(01)00298-5
更新日期:2001-09-30 00:00:00
abstract::Protein C (PC) is the central protein in a major antithrombotic regulatory mechanism. Hereditary deficiencies of PC are associated with thrombosis. Therapeutic PC replacement may be an important treatment if pure functional human protein C is available in sufficient quantity. Human PC has been produced on a commercial...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(90)90258-e
更新日期:1990-02-01 00:00:00
abstract::Bleeding is an important complication of heparin therapy. A number of low molecular weight heparin fractions produce less bleeding than standard heparin for an equivalent antithrombotic effect in experimental animals. Low molecular weight heparin fractions and fragments are produced by a number of different procedures...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(84)90265-2
更新日期:1984-09-15 00:00:00
abstract::Disseminated intravascular coagulation (DIC) is a severe complication of septic shock. Polymorphonuclear neutrophils (PMNs) may play a key role in septic shock-induced DIC via the release of neutrophils extracellular traps (NETs). NETs capture invading pathogens, but also act as a pro-coagulant surface at the interfac...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2019.09.036
更新日期:2019-11-01 00:00:00
abstract::Major orthopaedic surgery is associated with an increased risk of venous thromboembolism. Direct oral anticoagulants (DOACs) are recommended as thromboprophylactic agents after orthopaedic surgery. Although routine monitoring of DOACs in general is not required, measuring DOAC concentration may be necessary in clinica...
journal_title:Thrombosis research
pub_type: 临床试验,杂志文章
doi:10.1016/j.thromres.2016.01.012
更新日期:2016-03-01 00:00:00
abstract::We characterized a platelet specific alloantigen (Yukb). In immunoblotting, anti-Yukb antibody was found to react with both 110 kDa and 96 kDa bands under nonreducing condition. Immunoblotting followed by separation by two-dimensional electrophoresis (isoelectric focusing/SDS-PAGE) showed that the 96 kDa band had a pI...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(92)90136-x
更新日期:1992-07-15 00:00:00
abstract:INTRODUCTION:We conducted this prospective study in order to investigate the hypotheses that an alternative pathway for fibrinolysis is activated in patients who have undergone cardiopulmonary bypass (CPB) surgery and major abdominal surgery and that the levels of fibrin degradation products digested by polymorphonucle...
journal_title:Thrombosis research
pub_type: 临床试验,杂志文章
doi:10.1016/j.thromres.2006.07.009
更新日期:2007-01-01 00:00:00
abstract::Bovine myelin basic protein (BP) induced a shape change and endogenous phosphorylation of a 45,000 (45K) molecular weight protein of intact human platelets. This effect occurred rapidly over an effective concentration range of 5-100 microM BP. BP peptides encompassing residues 1-42, 43-88, and 89-169 from the BP molec...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(82)90090-1
更新日期:1982-03-15 00:00:00
abstract::Platelet aggregation induced by ADP and collagen was monitored in 25 patients with increased serum levels of triglycerides (7 HLPs type IIb, 14 type IV, 4 type V patients) and compared with a group of 10 normolipidaemic control persons. Platelet aggregation was studied simultaneously in platelet rich plasma (PRP) by b...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(86)90238-0
更新日期:1986-02-01 00:00:00
abstract:INTRODUCTION:Cancer is a major risk factor for developing venous thromboembolism (VTE). Plasma hypercoagulability is an established risk factor for cancer-related VTE. In addition, thrombocytosis and hyperreactive platelets have been implicated in VTE and cancer progression. Cirrhosis is associated with changes in plat...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2014.11.016
更新日期:2015-02-01 00:00:00
abstract::It has become customary to regard the various glycerophospholipids as quite similar, and the acyl groups are considered to have little influence on the behaviour of the lipids in membranes or metabolism. Nevertheless, a number of recent observations by the authors and others indicate a high degree of metabolic compart...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/0049-3848(92)90006-v
更新日期:1992-08-01 00:00:00
abstract::Lupus anticoagulants (LA) are IgG or IgM antibodies which prolong phospholipid-dependent coagulation tests. For the detection and quantitation of such antibodies, we have developed an ELISA with cephalin as the coating antigen. The sensitivity of this assay was compared to the activated partial thromboplastin time (AP...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(90)90323-5
更新日期:1990-01-15 00:00:00
abstract:INTRODUCTION:Ultrasound can accelerate clot dissolution in vitro and in vivo. We used an ex vivo canine shunt to investigate low frequency ultrasound effects on platelet-rich stent thrombosis. METHODS AND RESULTS:Nitinol stents were expanded to 2 mm in diameter in two perfusion chambers in a parallel shunt and exposed...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2003.10.023
更新日期:2003-01-01 00:00:00
abstract:INTRODUCTION:Previous studies have suggested the used of off-label recombinant factor VII (rFVIIa) increases the risk of thromboembolic events, but the effect of the dose of rFVIIa is not well described in the literature. MATERIALS AND METHODS:All adult patients that received off-label rFVIIa from 2005-2012 were inclu...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2014.02.026
更新日期:2014-05-01 00:00:00
abstract::To estimate the effect of dilazep in preventing restenosis after a transluminal angioplasty, we have attempted an animal experiment in which the efficacy of drug was tested on an angioplastic injury superimposed on to atherosclerotic lesions. Using a modification of Block's method, atherosclerotic lesions first were m...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(89)90122-9
更新日期:1989-06-15 00:00:00
abstract::Tissue factor, the cofactor for factor VIIa-catalyzed activation of factors IX and X, plays an important role in the initiation of hemostasis. However, the distribution of tissue factor in the body has not been defined until recently. In the present study frozen sections of non-malignant human tissues were immunostain...
journal_title:Thrombosis research
pub_type: 更正并重新发布的文章,杂志文章
doi:10.1016/0049-3848(90)90148-6
更新日期:1990-07-15 00:00:00
abstract:BACKGROUND:Thrombin is the most potent platelet activator, and achieves rapid platelet activation even in the presence of antiplatelet therapy. Since activated platelets respond stronger to additional stimuli, the extent of endogenous thrombin generation may in part be responsible for the reported response variability ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2013.04.008
更新日期:2013-07-01 00:00:00
abstract:INTRODUCTION:To validate the diagnostic criteria for disseminated intravascular coagulation (DIC) established by the Japanese Association for Acute Medicine (JAAM) at an early stage of trauma and to evaluate the hypothesis that the JAAM criteria can diagnose DIC with a higher sensitivity than the International Society ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2009.06.036
更新日期:2009-12-01 00:00:00
abstract:INTRODUCTION:The TF-FVIIa complex is the primary activator of coagulation. Elevated levels of microvesicle (MV) bearing tissue factor (TF)-dependent procoagulant activity are detectable in patients with an increased risk of thrombosis. Several methods have been described to measure MV TF activity but they are hampered ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2019.07.011
更新日期:2019-10-01 00:00:00
abstract::We have previously described a stimulus-specific defect in platelet aggregation in polycythaemia vera (PV) after stimulation with surface receptor dependent agonists such as platelet activating factor (PAF). In contrast, responses to phorbol myristate acetate (PMA) were normal. We now report that after PAF stimulation...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(98)00096-6
更新日期:1998-09-15 00:00:00
abstract:BACKGROUND:The recanalization rate in patients with deep venous thrombosis (DVT) of the legs treated with the direct oral anticoagulants (DOAC) is unknown. METHODS:In an Italian cohort, we investigated the rate of residual vein thrombosis (RVT) after three and/or six months in 352 patients with proximal DVT who had be...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2017.03.022
更新日期:2017-05-01 00:00:00
abstract:BACKGROUND:There is a need to identify and develop novel thrombolytic agents that can directly digest fibrin clots from various biological resources. OBJECTIVE:To clone, express, purify, and characterize a recombinant protease named rvFMP capable of cleaving fibrinogen, fibrin polymer, and cross-linked fibrin in human...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2020.04.040
更新日期:2020-07-01 00:00:00
abstract::Highly purified bovine von Willebrand protein (vWP) has been studied with two complementary techniques. Light scattering measurements on different samples give weight average molecular weights in the range from 7 to 13 million, radius of gyration between 75 and 140 nm and diffusion coefficient near 4.15 X 10(-8) cm2/s...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(85)90289-0
更新日期:1985-11-15 00:00:00
abstract::S-antigen (also named arrestin or 48K protein) is a protein abundant in photoreceptor cells of vertebrates and invertebrates. The presently known function of this protein in retina is to arrest the enzymatic cascade of phototransduction in retinal rods, through its binding to photoactivated and phosphorylated rhodopsi...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:
更新日期:1991-12-01 00:00:00
abstract::Cancer-associated arterial thrombotic events (ATEs) are increasingly recognized in specific malignancies and in association with the expanding armamentarium of novel chemotherapeutic agents. The improved cancer survival led to cardiovascular complications becoming clinically relevant many years after cancer diagnosis....
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2018.01.003
更新日期:2018-04-01 00:00:00
abstract:BACKGROUND:Brain tumor patients have an increased risk of venous thromboembolism (VTE). An important role in cancer-related VTE has been suggested for tissue factor (TF), the main initiator of the coagulation cascade. We conducted a prospective cohort study to determine whether expression levels of TF in brain tumors a...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2012.09.020
更新日期:2013-02-01 00:00:00
abstract::Three collagenases were purified from the culture medium of human skin fibroblasts using ammonium sulfate fractionation, ion-exchange chromatography and gel filtration. The cationic collagenase had a molecular weight of 42,000; two anionic collagenases had molecular weights of 63,000 and 115,000. Preincubation of the ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(84)90211-1
更新日期:1984-07-15 00:00:00
abstract::The influence of acute hypoxemia on blood fibrinolytic activity was investigated in 12 healthy males. Physiologically significant hypoxemia was produced by inspiration of 13% oxygen for 30 min. Six healthy males were exposed to hypoxemia at rest and 6 males to hypoxemia during exhaustive physical exercise on an ergocy...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(87)90222-2
更新日期:1987-02-15 00:00:00