Abstract:
:We have previously described a stimulus-specific defect in platelet aggregation in polycythaemia vera (PV) after stimulation with surface receptor dependent agonists such as platelet activating factor (PAF). In contrast, responses to phorbol myristate acetate (PMA) were normal. We now report that after PAF stimulation, using flow cytometry, the amount of fibrinogen bound to its receptor was significantly lower in PV platelets with a median MFI of 6.0 (range 4.1-17.3) compared to controls, 12.8 (range 8-21.3; n=11; p<0.01). We found no evidence of preactivation of PV platelets. Quantitative analysis of GPIIIa gave a significantly lower number of GPIIIa on resting PV platelets, 14300 subunits of GPIIIa (range 8500-15500) vs. 19800 for controls (range 13400-26800; n=12; p<0.01). Both patients and controls increased their number of receptors on the cell surface after stimulation with PAF and PMA, but the significant difference in the number of receptors per cell remained. Indirect evaluation of PAF receptor function showed that activation of CD 62 did not differ in PV and controls after PAF stimulation. Additionally, although the basal level of serotonin in platelet-rich plasma was significantly lower in PV, there was a threefold increase of the basal level after stimulation with PAF for both PV and control platelets, also indicating a normal interaction of PAF with its receptor. Although our results indicate both an impaired PAF induced aggregation in PV and a lower number of GPIIb/IIIa complexes on single platelets, whether these phenomena are related remains uncertain.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Le Blanc K,Lindahl T,Rosendahl K,Samuelsson Jdoi
10.1016/s0049-3848(98)00096-6subject
Has Abstractpub_date
1998-09-15 00:00:00pages
287-95issue
6eissn
0049-3848issn
1879-2472pii
S0049-3848(98)00096-6journal_volume
91pub_type
杂志文章abstract::To estimate the effect of dilazep in preventing restenosis after a transluminal angioplasty, we have attempted an animal experiment in which the efficacy of drug was tested on an angioplastic injury superimposed on to atherosclerotic lesions. Using a modification of Block's method, atherosclerotic lesions first were m...
journal_title:Thrombosis research
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doi:10.1016/0049-3848(89)90122-9
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journal_title:Thrombosis research
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abstract:BACKGROUND:While post thrombotic syndrome (PTS) is increasingly recognized as a frequent and potentially serious complication of deep vein thrombosis (DVT) in children, limited information is available regarding predictors of PTS. METHODS:Using the Mayo Clinic Master Diagnostic Index, all pediatric patients (age 0 to ...
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doi:10.1016/0049-3848(94)90142-2
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doi:10.1016/0049-3848(85)90297-x
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更新日期:2009-04-01 00:00:00
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journal_title:Thrombosis research
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doi:10.1016/j.thromres.2007.06.013
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doi:10.1016/j.thromres.2007.10.002
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更新日期:1982-12-15 00:00:00
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journal_title:Thrombosis research
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doi:10.1016/j.thromres.2012.06.016
更新日期:2012-09-01 00:00:00
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journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2006.10.020
更新日期:2007-01-01 00:00:00
abstract::Cardiovascular diseases are one of the leading causes of morbidity and mortality in industrialized countries, and although many processes play a role in the development of vascular disease, thrombosis is the primary event that precipitates stroke and acute coronary syndromes. The blood platelets are of significant imp...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/j.thromres.2011.09.026
更新日期:2012-02-01 00:00:00
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journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2003.10.023
更新日期:2003-01-01 00:00:00
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journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(85)90211-7
更新日期:1985-06-15 00:00:00
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journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2014.09.030
更新日期:2014-12-01 00:00:00
abstract::We investigated the alteration of heparan sulfate proteoglycans induced by thrombin in cultured vascular endothelial cells. Heparan sulfate proteoglycans, which were metabolically labeled with [3H] glucosamine and [35S] sulfate, were isolated by DEAE-Sephacel ion-exchange chromatography and characterized by molecular ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(97)00257-0
更新日期:1997-11-01 00:00:00
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journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2014.08.016
更新日期:2014-11-01 00:00:00