Abstract：
BACKGROUND:Clauss fibrinogen assay (CFA) is widely used as a screening test to detect fibrinogen disorders. However, CFA alone cannot distinguish quantitative and qualitative defects because it depends on functional fibrinogen activity (Ac), and fibrinogen antigen (Ag) determination is required to classify fibrinogen disorders. OBJECTIVES:To establish a novel approach to classify fibrinogen disorders, we investigated the potential of clot waveform analysis (CWA) of CFA and searched for a surrogate marker for fibrinogen Ag. MATERIALS AND METHODS:We analyzed CWA parameters obtained from CFA using plasma from normal patients (n = 91) and those with fibrinogen disorders (n = 27, including 15 hypofibrinogenemia, 6 dysfibrinogenemia and 6 hypodysfibrinogenemia) with a CS-5100 autoanalyzer. RESULTS:We found that maximum coagulation velocity (Min1) levels were most strongly correlated with fibrinogen Ag in both normal and fibrinogen disorders. Hence, Min1 appeared to function as a surrogate for fibrinogen Ag. Although the Ac/Min1 ratio did not simply reflect the measured Ac/Ag ratio, we found that the Ac/Min1 ratio was significantly higher than normal in hypofibrinogenemia and hypodysfibrinogenemia, but not in dysfibrinogenemia. On the other hand, we could distinguish type II deficiency from type I using estimated fibrinogen Ag (eAg) predicted from Min1. The Ac/eAg ratios of dysfibrinogenemia and hypodysfibrinogenemia were significantly lower than those of normal and hypofibrinogenemia. CONCLUSION:The CWA of CFA could distinguish fibrinogen disorders using a combination of Ac/Min1 and Ac/eAg values. This analysis allows the qualitative detection of fibrinogen disorder easily and represents a novel screening test for fibrinogen disorders.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Suzuki A,Suzuki N,Kanematsu T,Shinohara S,Arai N,Kikuchi R,Matsushita Tdoi
10.1016/j.thromres.2018.12.018subject
Has Abstractpub_date
2019-02-01 00:00:00pages
98-103eissn
0049-3848issn
1879-2472pii
S0049-3848(18)30667-4journal_volume
174pub_type
杂志文章abstract：INTRODUCTION:Maximal exercise may be a trigger for cardiovascular events. The aim of the study was to investigate changes in blood coagulation and fibrinolysis following maximal short-term exercises with different durations up to 90 s. METHODS:A total of 15 healthy nonsmokers underwent three isokinetic maximal tests o...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(03)00283-4
更新日期：2003-03-15 00:00:00
abstract：:A significantly higher platelet malondialdehyde-like material (MDA--LM) content after physical and N-ethylmaleimide (NEM) stimulation is found in chronic ischemic heart disease (CHD) patients when compared to the control group. In subjects under aspirin treatment "in vivo" (1 gr/day) no difference is found between CHD...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(82)90305-x
更新日期：1982-09-01 00:00:00
abstract：BACKGROUND:Systemic thrombolysis rapidly improves right ventricular (RV) dysfunction in patients with acute pulmonary embolism (PE) but is associated with major bleeding complications in up to 20%. The efficacy of low-dose, catheter-directed ultrasound-accelerated thrombolysis (USAT) on the reversal of RV dysfunction i...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2011.05.014
更新日期：2011-08-01 00:00:00
abstract：:The brain and the placenta synthesize identical peptides and proteins, such as brain-derived neurotrophic factor, oxytocin, vascular endothelial growth factor, cortisol, and matrix metalloproteinases. Given the promiscuity between neurochemistry and the mechanism of placentation, it would be expected that mental disor...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/S0049-3848(15)50446-5
更新日期：2015-02-01 00:00:00
abstract：:Diabetes is associated with multiple disorders including metabolic, cellular and blood disturbances leading to vascular complications. Increased circulating levels of platelet-leukocyte aggregates (PLA) have been described in several thrombotic diseases. In this study, we have evaluated circulating PLA in diabetic pat...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2007.07.016
更新日期：2008-01-01 00:00:00
abstract：INTRODUCTION:In the pathogenesis of sepsis-induced acute lung injury (ALI), the crosstalk between inflammation and coagulation plays a pivotal role. The aim of this study was to investigate the role of Rho kinase (ROCK) inhibitor in alleviating pulmonary inflammation and coagulation in lipopolysaccharide (LPS)-induced ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2016.12.020
更新日期：2017-02-01 00:00:00
abstract：BACKGROUND:Although excess platelet activation, as indicated by increased plasma beta thromboglobulin (beta-TG), has been shown in pregnancy-induced hypertension (PIH), platelet adhesion, platelet morphology and a comparison of platelet and soluble (plasma) levels of the adhesion molecules P-selectin (pPsel and sPsel, ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2005.01.009
更新日期：2005-01-01 00:00:00
abstract：INTRODUCTION:Dabigatran (Dabi) is not routinely monitored. However, in emergency cases quantitative assessment is required and laboratories must provide suitable tests at all hours. Little is known about Dabi effects on thrombin generation. MATERIALS AND METHODS:Patient samples (n=241) were analyzed for functional Dab...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2015.04.032
更新日期：2015-07-01 00:00:00
abstract：BACKGROUND AND PURPOSE:Miniplasmin was a des-kringle variant of plasminogen with potential pharmacological application. We investigated the thrombolytic effect of miniplasmin in a canine model of femoral artery thrombosis. METHODS:In anesthetized dogs, a stable occlusive thrombus was formed by mechanical and electroly...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2008.01.007
更新日期：2008-01-01 00:00:00
abstract：:RS-5186, sodium 6-[2-[1-(1H)-imidazolyl]methyl-4,5-dihydrobenzo[b]thiophene]- carboxylate, inhibited platelet thromboxane A2 (TXA2) synthetase with IC50 values of 6 nM and 13 nM for human and rabbit microsomes, respectively. It had a selectivity for TXA2 synthetase 10(5)-fold greater than that for cyclooxygenase, PGI2...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(88)90116-8
更新日期：1988-09-01 00:00:00
abstract：BACKGROUND:The aim of this study was to describe platelet aggregation characteristics by multiple electrode aggregometry (MEA) and to evaluate MEA for its potential to detect platelet dysfunction and response to anti-aggregatory drugs in patients with myeloproliferative disorders (MPD). METHODS:We compared the platele...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2010.06.014
更新日期：2010-09-01 00:00:00
abstract：:Highly purified bovine von Willebrand protein (vWP) has been studied with two complementary techniques. Light scattering measurements on different samples give weight average molecular weights in the range from 7 to 13 million, radius of gyration between 75 and 140 nm and diffusion coefficient near 4.15 X 10(-8) cm2/s...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(85)90289-0
更新日期：1985-11-15 00:00:00
abstract：:Using the standard turbidimetric method of platelet aggregation and quantitation of platelet secretion with 14C-Serotonin, we have examined the responsiveness of the platelets of mongrel dogs to arachidonic acid (AA), and the thromboxane agonist U46619 in the presence and absence of a subthreshold concentration of epi...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(89)90300-9
更新日期：1989-09-15 00:00:00
abstract：INTRODUCTION:Severe thrombocytopenia (≤50×10(9) platelets/L) is often the consequence of hematological malignancies and intensive chemotherapy. The risk of clinically significant bleeding is increased in these patients, despite the use of prophylactic platelet transfusions. The fact that there is no clear correlation b...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/S0049-3848(16)30125-6
更新日期：2016-04-01 00:00:00
abstract：:Danazol, a synthetic androgen reported to increase factor VIII and IX activity levels, was given to 6 hemophiliacs. With danazol therapy (600 mg/da) the APTTs shortened by 30-45% of pre-treatment times. However, the activity levels of the deficient factors did not increase significantly nor consistently with the APTT ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(85)90126-4
更新日期：1985-07-01 00:00:00
abstract：:β(2)glycoprotein I (β(2)GPI) is the major antigen in the antiphospholipid syndrome. It has been shown that β(2)GPI can adapt to different conformations, a circular, a S-shaped and a J-shaped conformation. In literature anticoagulant properties of β(2)GPI have been indicated, though there is no consensus on how β(2)GPI...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2012.08.269
更新日期：2012-10-01 00:00:00
abstract：:Antithrombin III (AT III) is increased in situations where there is increased platelet turnover. Plasma AT III levels measured in 39 renal transplant recipients were significantly higher than in 20 healthy subjects (p less than 0.001) and 20 patient controls (p less than 0.025). AT III levels were significantly correl...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(85)90147-1
更新日期：1985-05-01 00:00:00
abstract：BACKGROUND:Atherosclerosis occurs as a result of a chronic inflammatory response in the arterial wall associated with an increased uptake of low-density lipoprotein by macrophages and the subsequent transformation of this lipoprotein into foam cells. It has been found that miR-188-3p can suppress autophagy and myocardi...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2018.09.043
更新日期：2018-11-01 00:00:00
abstract：BACKGROUND:Coronary collateral circulation (CCC) plays an important role in protecting myocardium from ischemic damage. The studies on factors which impact on CCC might be of great clinical interest. The aim of the present study was to evaluate endothelin-1 (ET-1) as a potential predictor for poor or good CCC in patien...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2016.04.007
更新日期：2016-06-01 00:00:00
abstract：INTRODUCTION:To test the hypothesis that the platelet microparticle (PMP) and endothelial microparticle (EMP) may contribute to the hypercoagulability associated with microvascular thrombosis in patients with nontraumatc osteonecrosis of the femoral head (ONFH). MATERIALS AND METHODS:The study comprised 46 patients wh...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2008.04.001
更新日期：2008-01-01 00:00:00
abstract：BACKGROUND:Muromonab-CD3 is a murine monoclonal antibody (MoAb) that is used in the prophylaxis and treatment of acute graft rejection. Activation of coagulation and fibrinolysis following anti-CD3 administration have been reported in some patients to lead to irreversible intragraft thrombosis. DESIGN AND METHODS:We h...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(01)00396-6
更新日期：2001-12-15 00:00:00
abstract：:Forty women undergoing surgery under general anaesthesia for hyperplasia mammae were randomized to treatment with low dose heparin (5000 IU twice daily) or not. Preoperatively, and repeated on the 3. postoperative day, assays of euglobulin clot lysis time (ELT) after venous stasis, tissue plasminogen activator (t-PA) ...
journal_title：Thrombosis research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/0049-3848(87)90318-5
更新日期：1987-03-01 00:00:00
abstract：:Effects of vascular constriction on thrombotic occlusion was evaluated using rat mesenteric arteries and video-recording system attached to the microscope. Topical application of norepinephrine of 1, 10 and 100 micrograms/ml reduced the arterial diameter dose dependently from 297 +/- 41 mu to 166 +/- 50, 87 +/- 18 and...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(86)90178-7
更新日期：1986-10-01 00:00:00
abstract：OBJECTIVE:To date, only a few case studies have reported occurrence of thrombosis in patients with VonWillebrand disease (VWD). No studies have looked at its incidence in this patient population. The aim of this study was to test our hypothesis that decreased VonWillebrand factor (VWF) levels confer a protective effect...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2012.09.005
更新日期：2012-11-01 00:00:00
abstract：BACKGROUND:The methodology of thromboprophylaxis post minimally invasive esophagectomy (MIE) is unclear. Thus, we compared the efficacy and safety of fondaparinux and nadroparin on the prophylaxis of venous thromboembolism (VTE) after MIE. MATERIALS AND METHODS:We conducted a randomized, double-blind, treatment-contro...
journal_title：Thrombosis research
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.thromres.2018.04.001
更新日期：2018-06-01 00:00:00
abstract：:Anticoagulant properties of three sulfated compounds prepared from xylans isolated from corn cobs, larchwood and oatspelts were compared with heparin and sodium pentosan polysulfate (SP-54) by studying their effects on activated partial thromboplastin time (APTT), prothrombin time (PT) and thrombin time (TT) using poo...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(87)90148-4
更新日期：1987-08-01 00:00:00
abstract：:Owing to the beneficial health effects on human cardiovascular system, soybeans and soy-related products have been a focus of intensive research. Soy isoflavones are known to be primarily responsible for the soy-related biological effects including anti-platelet activity but its in vivo relevancy has not been fully ve...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2013.01.002
更新日期：2013-03-01 00:00:00
abstract：:This prospective randomized study investigated the influence of normotensive and hypotensive general anesthesia on platelet aggregability, intraoperative blood loss and parameters of plasmatic coagulation during extensive orthognathic surgery. A total of 30 patients were randomly allocated for either normotensive anes...
journal_title：Thrombosis research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0049-3848(01)00316-4
更新日期：2001-08-01 00:00:00
abstract：BACKGROUND:SARS-CoV-2 infection has noted derangements in coagulation markers along with significant thrombotic complications. Post-mortem examinations show severe endothelial injury and widespread thrombotic microangiopathy in the pulmonary vasculature. Early reports describing the use of prophylactic anticoagulation ...
journal_title：Thrombosis research
pub_type： 评论,信件
doi：10.1016/j.thromres.2020.09.030
更新日期：2020-12-01 00:00:00
abstract：:Ethamsylate possesses antihemorrhagic properties, but whether or not it directly activates blood platelets is unclear. Here we investigated the platelet activation potential of ethamsylate, by measuring membrane P-selectin expression with flow cytometry in human whole blood and also by immunofluorescence imaging of is...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(02)00353-5
更新日期：2002-09-15 00:00:00
