Abstract：
INTRODUCTION:The kinetics of the thrombin inhibition by heparin cofactor II (HCII) and antithrombin (AT) have been studied as a function of the concentration of a dermatan sulfate (DS) from the skin of the ray Raja radula. MATERIALS AND METHODS:The initial concentrations of inhibitor (I), HCII or AT, and thrombin (E) were set at equimolecular levels (3.10(-9) M). Analysis of the experimental data obtained for DS concentrations ranging from 10(-8) to 10(-4) M was performed according to a previously described model in which DS binds quickly to the inhibitor and forms a complex more reactive than the free inhibitor towards thrombin. RESULTS:The apparent rate constant of the thrombin inhibition, k(app), by either HCII or AT, increased in a concentration-dependent manner for DS concentrations up to 10(-5) M or 10(-6) M, respectively. At higher DS concentrations, k(app) remained unchanged for thrombin inhibition by HCII whereas a decrease in k(app) was observed for the thrombin-AT reaction. The dissociation constant of the polysaccharide-inhibitor complex, K(DSI), and the rate constant of the thrombin inhibition by this complex, k, were (7.81+/-0.75).10(-7) M and (2.84+/-0.42).10(9) M(-1).min(-1), whereas they were (4.93+/-0.31).10(-7) M and (2.47+/-0.28).10(8) M(-1).min(-1), when the inhibitor was either HCII or AT, respectively. CONCLUSION:DS from ray skin catalyzes the thrombin inhibition by HCII or AT primarily by forming a DS-inhibitor complex more reactive than the free inhibitor towards the protease. The affinity of DS for HCII was approximately 2-fold higher whereas the catalyzed reaction rate constant was approximately 20-fold higher when compared to AT.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Ben Mansour M,Dhahri M,Vénisse L,Jandrot-Perrus M,Chaubet F,Maaroufi RMdoi
10.1016/j.thromres.2008.10.003subject
Has Abstractpub_date
2009-04-01 00:00:00pages
902-8issue
6eissn
0049-3848issn
1879-2472pii
S0049-3848(08)00488-Xjournal_volume
123pub_type
杂志文章abstract：:Thrombus formation was electrically induced in the left common carotid artery of rats with preoccluded both vertebral and right carotid arteries. Following thrombus induction, animals became unresponsive and lost their righting reflex. The loss of righting reflex was observed when cortical tissular pO2 reached 55 +/- ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(87)90340-9
更新日期：1987-10-01 00:00:00
abstract：:Endothelial cells from human umbilical vein were incubated in vitro with thrombin in the absence or presence of unfractionated heparin (UFH), low molecular weight heparin (LMWH) or dermatan sulfate (DS) to clarify the effect of these glycosaminoglycans on thrombin-stimulated prostaglandin I2 (PGI2) production. Althoug...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(90)90065-k
更新日期：1990-02-15 00:00:00
abstract：INTRODUCTION:It has been widely accepted that genetic factors were the major sources of the variation in warfarin dose. This study is intended to investigate whether the 3261G>A variation in GGCX gene influences stable warfarin dose in Chinese patient population. MATERIALS AND METHODS:A total of 217 patients with stab...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2010.10.027
更新日期：2011-02-01 00:00:00
abstract：PURPOSE:Due to its specific pharmacokinetic profile, tinzaparin, a low-molecular-weight heparin (LMWH), appears not to be associated with anti-factor Xa accumulation. Our meta-analysis aimed at determining whether long-term curative doses of tinzaparin is a valuable alternative to vitamin K antagonists (VKA) for the tr...
journal_title：Thrombosis research
pub_type： 杂志文章,meta分析
doi：10.1016/j.thromres.2012.08.290
更新日期：2012-12-01 00:00:00
abstract：:Activated factor X (FXa) and thrombin can up-regulate gene expression of connective tissue growth factor (CTGF/CCN2) on fibroblasts. Since tissue factor (TF) is expressed on these cells, we hypothesized that they may assemble the prothrombinase complex leading to CTGF/CCN2 upregulation. In addition, the effect of thro...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2011.08.009
更新日期：2012-06-01 00:00:00
abstract：INTRODUCTION:The low-molecular-weight heparins have been demonstrated to have antiangiogenic effects in various assays. We aimed to demonstrate and compare the antiangiogenic effects of four types of commercially available low-molecular weight heparins in the chick embryo chorioallantoic membrane model. MATERIALS AND ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2011.05.005
更新日期：2011-10-01 00:00:00
abstract：:Lipoprotein-associated coagulation inhibitor (LACI) is a plasma-derived protein that inhibits the tissue factor/factor VIIa/calcium/phospholipid complex in a factor Xa-dependent manner. Recombinant LACI (rLACI) added exogenously to plasma prolonged the activated partial thromboplastin time (APTT) and prothrombin time ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(91)90120-l
更新日期：1991-10-15 00:00:00
abstract：:Von Willebrand Factor is a multimer produced by endothelial cells and megakaryocytes, being stored in intracellular organelles, such as the Weibel-Palade bodies and alpha-granules in endothelial cells and platelets, respectively. This molecule acts as a carrier protein for factor VIIIc, involved in the intrinsic pathw...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(99)00122-x
更新日期：2000-01-15 00:00:00
abstract：INTRODUCTION:Lung cancer is the leading cause of cancer-related death worldwide. Small cell lung cancer (SCLC) is thereby a highly aggressive neuroendocrine carcinoma representing about 15% of all lung cancer cases. Due to the highly metastatic behavior and multidrug resistance, the long-term survival of patients is ve...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/S0049-3848(16)30166-9
更新日期：2016-04-01 00:00:00
abstract：INTRODUCTION:Patients with nonvalvular atrial fibrillation are at increased risk for systemic embolism, predominantly disabling stroke. To study how stroke and mortality rates vary with different degrees of anticoagulation reflected by the international normalised ratio (INR) we critically assess information from diffe...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2004.11.025
更新日期：2006-01-01 00:00:00
abstract：INTRODUCTION:The A-subunit of blood coagulation factor XIII is a pro-transglutaminase, which cross-links α- and γ-fibrin-chains in its activated form. Selective inhibitors against FXIII-A may be desirable drugs to prevent the development of thromboses. Animal models are generally used for proof of principle and for tox...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2013.02.008
更新日期：2013-05-01 00:00:00
abstract：INTRODUCTION:Platelets and the coagulation system may be involved in the pathogenesis of pre-eclampsia. We investigated whether platelet and coagulation activation markers, are elevated in pre-eclampsia. MATERIALS/METHODS:Case-control study in which activated platelets, platelet-monocyte/ neutrophil aggregates, platel...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2009.09.013
更新日期：2010-03-01 00:00:00
abstract：:We characterized a platelet specific alloantigen (Yukb). In immunoblotting, anti-Yukb antibody was found to react with both 110 kDa and 96 kDa bands under nonreducing condition. Immunoblotting followed by separation by two-dimensional electrophoresis (isoelectric focusing/SDS-PAGE) showed that the 96 kDa band had a pI...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(92)90136-x
更新日期：1992-07-15 00:00:00
abstract：:To compare Canola a terrestrial (n-3) oil with fish oil (n-3) and common vegetable oil (n-6) in decreasing serum lipids and platelet aggregation, various oils were included in Chow-based diets and fed to rats in an eight-weeks feeding trial. The Chow diet contained 2% cholesterol along with either Canola, menhaden, sa...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(90)90420-h
更新日期：1990-08-01 00:00:00
abstract：BACKGROUND:Treatment of deep-vein thrombosis (DVT) with a once-daily regimen of enoxaparin, rather than a continuous infusion of unfractionated heparin (UFH) is more convenient and allows for home care in some patients. This study was designed to compare the efficacy and safety of these two regimens for the treatment o...
journal_title：Thrombosis research
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：10.1016/j.thromres.2004.05.009
更新日期：2004-01-01 00:00:00
abstract：INTRODUCTION:High RDW values are associated with adverse prognosis in many clinical conditions including short and medium term outcome of patients with ST Elevation Myocardial Infarction (STEMI). The aim of the present study was to evaluate the association between RDW and long term mortality in STEMI patients undergoin...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2014.08.016
更新日期：2014-11-01 00:00:00
abstract：:In the treatment of unstable coronary artery disease, direct thrombin inhibitors have shown no or only limited benefit as compared with heparin, despite theoretical advantages. One explanation may be that the direct thrombin inhibitors to a greater extent than heparin have an inhibiting effect on the generation and ac...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(99)00029-8
更新日期：1999-07-15 00:00:00
abstract：INTRODUCTION:Vitamin K antagonists are often used for anticoagulant treatment in hip fracture patients. The optimal handling with such anticoagulants is unclear. We aimed to determine when anticoagulation reversal occurred after vitamin K administration and how often prothrombin complex concentrates (PCCs) were adminis...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2013.10.031
更新日期：2014-01-01 00:00:00
abstract：:Fucoidan [sulfated poly (L-fucopyranose)] was compared with 6-aminohexanoic acid (6-AH) or CNBr-cleaved fibrinogen (CNBr-Fbg) alone or in combination in enhancing the activation of glutamic plasminogen (Glu-Plg) or lysine plasminogen (Lys-Plg) by two-chain tissue plasminogen activator (t-PA) or LMwt-urokinase or by st...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(95)00111-4
更新日期：1995-08-01 00:00:00
abstract：:The effects of locally deposited fibrin and of tranexamic acid-induced antifibrinolysis on forming granulation tissue were studied in the light of a recently developed method for treatment of postoperative fistulas by occlusion with a fibrin clot. Perforated teflon cylinders, either empty or fibrin-filled, were implan...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(84)90152-x
更新日期：1984-01-01 00:00:00
abstract：:Although significant advances in hemophilia treatment have improved patient outcomes and quality of life, one of the greatest complications in severe hemophilia A is the development of anti-Factor VIII (FVIII) antibodies that inhibit FVIII activity in almost 30% of previously untreated patients (PUPs). Inhibitors make...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2018.05.027
更新日期：2018-08-01 00:00:00
abstract：:Bone marrow transplantation (BMT) is the only curable option for thalassemia major, β-thalassemia/HbE. However, some patients still have the risk of hypercoagulable complications. We used a whole blood flow cytometric analysis to measure the circulating microparticle (MP) levels, activated platelets, and leukocyte-pla...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2018.07.007
更新日期：2018-09-01 00:00:00
abstract：:Cancer is frequently associated with activation of blood coagulation, which in turn has been suggested to promote tumor growth and metastasis. Indeed, low molecular weight heparin treatment significantly prolongs the survival of a wide variety of patients with cancer. Based on this notion that anticoagulant treatment ...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/S0049-3848(12)70022-1
更新日期：2012-04-01 00:00:00
abstract：:Human platelets contain a single membrane glycoprotein which is susceptible to thrombin proteolysis, glycoprotein V. We have purified 1 mg of glycoprotein V from 10(13) platelets using a combination of gel filtration, hydroxylapatite and ion-exchange chromatographies. Glycoprotein V has a blocked amino-terminus. Follo...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(89)90113-8
更新日期：1989-01-01 00:00:00
abstract：INTRODUCTION:The standard dabigatran etexilate dosage for prevention of venous thromboembolism (VTE) after elective total hip or knee replacement (THR/TKR) is 220mg once daily (qd), with 150mg qd for patients with moderate renal impairment. As clinical trial experience in patients with moderate renal impairment was lim...
journal_title：Thrombosis research
pub_type： 临床试验,杂志文章
doi：10.1016/j.thromres.2016.05.014
更新日期：2016-07-01 00:00:00
abstract：INTRODUCTION:Z4A5 is a novel peptide that inhibits platelet aggregation and formation of platelet thrombi, but the mechanism of its anti-platelet effects remains unknown. This study explores the anti-platelet effect and mechanism of Z4A5. METHODS:We investigated the anti-platelet activity of Z4A5 on platelet aggregati...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2012.02.047
更新日期：2012-05-01 00:00:00
abstract：:The effect of red wine drinking was tested on fibrinolytic parameters and blood cells in nine healthy students at rest and after acute exercise. The subjects were randomly assigned in a crossover design to one of three treatment regimes: control situation, low-dose wine group, and high-dose wine group. Blood samples w...
journal_title：Thrombosis research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0049-3848(99)00120-6
更新日期：1999-12-01 00:00:00
abstract：:Fractalkine/CX3CR1 pathway is considered a major modulator of atherosclerosis. In the present study, expression of CX3CR1 on PBMCs/monocytes of healthy individuals and coronary artery diseased patients was initially assessed by flow cytometry. Effects of pre-inflammatory cytokines interferon (INF)-gamma and tumor necr...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2007.04.005
更新日期：2007-01-01 00:00:00
abstract：:DMP 728 is a potent and specific platelet GPIIb/IIIa antagonist. Like all GPIIb/IIIa antagonists, DMP 728 has a steep dose-response relationship in inhibiting platelet aggregation. In this study the relationships between receptor occupancy, platelet aggregation and bleeding time was determined in anesthetized dogs aft...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(95)00029-1
更新日期：1995-03-15 00:00:00
abstract：INTRODUCTION:Dermatan sulfate, a sulfated glycosaminoglycan, acts as an anticoagulant by accelerating the inhibition of thrombin by heparin cofactor II. MATERIALS AND METHODS:A low molecular mass dermatan sulfate was obtained by peroxy-radical depolymerization from a parent dermatan sulfate. Chemical characterization ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2007.09.001
更新日期：2008-01-01 00:00:00
