Abstract:
:Fucoidan [sulfated poly (L-fucopyranose)] was compared with 6-aminohexanoic acid (6-AH) or CNBr-cleaved fibrinogen (CNBr-Fbg) alone or in combination in enhancing the activation of glutamic plasminogen (Glu-Plg) or lysine plasminogen (Lys-Plg) by two-chain tissue plasminogen activator (t-PA) or LMwt-urokinase or by streptokinase. Fucoidan enhanced the t-PA activation of Glu-Plg or Lys-Plg at Plg concentrations greater than 75nM, while stimulation by CNBr-Fbg of t-PA activation followed saturation kinetics of Michaelis-Menton. During t-PA activation of Glu-Plg, a high degree of synergism was observed between 6-AH and fucoidan while the enhancement by CNBr-Fbg was not influenced by fucoidan and was reversed by 6-AH. Fucoidan alone at higher concentrations was effective in enhancing the activation of Glu-Plg by urokinase while the combination of fucoidan and 6-AH showed additive effect in enhancing the activation of Lys-Plg. The activation of Glu-Plg by streptokinase was reversed by fucoidan in a manner similar to that reported for 6-AH. The results are interpreted to suggest that CNBr-Fbg and 6-AH compete with each other for the same lysine binding sites (LBS) on the Plg molecule while fucoidan acted synergistically with 6-AH in enhancing the t-PA activation of Glu-Plg by a different mechanism. The double reciprocal plot for the interaction of Glu-Plg and urokinase also showed a significantly higher affinity between the two in presence of fucoidan.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Doctor VM,Hill C,Jackson GJdoi
10.1016/0049-3848(95)00111-4subject
Has Abstractpub_date
1995-08-01 00:00:00pages
237-47issue
3eissn
0049-3848issn
1879-2472pii
0049-3848(95)00111-4journal_volume
79pub_type
杂志文章abstract::There is growing evidence on the superior efficacy and safety of low molecular weight heparins (LMWHs) over unfractionated heparin (UFH) for the treatment of both venous and arterial thromboembolism. Heparin exerts its function by potentiating antithrombin and by mobilizing tissue factor pathway inhibitor (TFPI) into ...
journal_title:Thrombosis research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0049-3848(98)00079-6
更新日期:1998-08-15 00:00:00
abstract::The enzymatic and fibrinolytic properties of glycosylated and nonglycosylated recombinant human pro-urokinase (pro-UK) produced in yeast Pichia pastoris were characterized and compared with those of Escherichia coli and mammalian cell-derived pro-UK. Among the five different forms of pro-UK, the yeast glycosylated pro...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(00)00349-2
更新日期:2000-12-01 00:00:00
abstract:BACKGROUND:There is a perception in the orthopaedic and thromboembolism community that the incidence of deep vein thrombosis (DVT) has decreased in patients undergoing total knee arthroplasty (TKA) or total hip arthroplasty (THA). OBJECTIVES:To assess the incidence of DVT with warfarin thromboprophylaxis over time in ...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/j.thromres.2008.05.005
更新日期:2008-01-01 00:00:00
abstract::Blackfoot disease is a unique endemic and chronic progressive arteriosclerotic vascular disease in southwest area of Taiwan. In this study, we determined the plasma levels of tissue plasminogen activator (tPA), plasminogen activator inhibitor (PAI), urokinase plasminogen activator (uPA), and von Willebrand factor (vWF...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(93)90188-t
更新日期:1993-11-01 00:00:00
abstract::Using a stable cross-linked SAK/plg complex, the effects of alpha 2-plasmin inhibitor on plasminogen activation by SAK were investigated. alpha 2-Plasmin inhibitor inhibited dose-dependently plasminogen activation by the SAK/plg complex. When FCB-2 or EACA was added to the reaction mixture of SAK/plg complex and alpha...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(94)90191-0
更新日期:1994-10-15 00:00:00
abstract::Retrievable filters are a new generation of inferior vena cava (IVC) filters and represent an attractive option because they may be either left in place permanently or safely retrieved after a quite long period when they become unnecessary. In this review the currently available literature regarding retrievable IVC fi...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/j.thromres.2007.06.017
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND:Clauss fibrinogen assay (CFA) is widely used as a screening test to detect fibrinogen disorders. However, CFA alone cannot distinguish quantitative and qualitative defects because it depends on functional fibrinogen activity (Ac), and fibrinogen antigen (Ag) determination is required to classify fibrinogen d...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2018.12.018
更新日期:2019-02-01 00:00:00
abstract::Four novel polymorphisms were identified in the fibrinogen gene cluster. Three of them were localized in the promoter regions of the Aalpha-chain (alpha -128 C/G, alpha -58 G/A) or the gamma-chain (gamma -239 A/G) gene, while the remaining one was identified in intron 9 of the gamma-chain gene (gamma 7792 C/T). Genoty...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(01)00329-2
更新日期:2001-08-15 00:00:00
abstract:BACKGROUND:Although risk of hospital-associated venous thromboembolism (HA-VTE) differs between critically and non-critically ill children, studies to date have not led to distinct, pragmatic risk scores. OBJECTIVE:To determine risk factors for HA-VTE in critically ill children not undergoing cardiothoracic surgery, i...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2015.04.036
更新日期:2015-10-01 00:00:00
abstract::Heparin is known to exert its antithrombotic effects by accelerating the effect of antithrombin (AT) and by mobilizing tissue factor pathway inhibitor (TFPI) into the circulation from vascular endothelium. Heparin treatment has been reported to decrease FVIIa activity by 40%; this was suggested as a new antithrombotic...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(00)00343-1
更新日期:2000-12-01 00:00:00
abstract::The placental transfer of Org 10172, a low-molecular weight heparinoid, was determined in 12 awake late-pregnant guinea pigs. Nine animals receiving placebo served as controls. After 5 days i.v. treatment with Org 10172 (2 x 300 anti-Xa U/kg/day), anti-Xa activity in fetal plasma amounted to 2.4% of the maternal conce...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(86)90002-2
更新日期:1986-11-01 00:00:00
abstract::Clot lysis induced by streptokinase has been studied and the influences of pH and the concentrations of plasminogen, fibrinogen and buffer composition investigated. Variations in the concentrations of plasminogen strongly effect the SK apparent activity, maxima being obtained at different plasminogen concentrations li...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(91)90071-4
更新日期:1991-12-15 00:00:00
abstract:INTRODUCTION:Endothelium-derived nitric oxide (NO) is synthesized from l-arginine by endothelial nitric oxide synthase (eNOS) encoded by the eNOS3 gene on chromosome 7. The effects of the eNOS polymorphisms with the risk of coronary artery disease are conflicting. In this study, we investigated the association of the e...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2006.06.005
更新日期:2007-01-01 00:00:00
abstract:INTRODUCTION:Growth differentiation factor-15 (GDF-15) has been identified as a predictor in cardiovascular diseases and acute pulmonary embolism. However, the association of GDF-15 and deep venous thrombosis (DVT) remains unclear. This study aimed to investigate levels of GDF-15 in patients with DVT, and determine its...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2020.01.022
更新日期:2020-03-01 00:00:00
abstract::A simple and discriminating assay for the determination of the fast-acting plasminogen activator inhibitor (PAI) activity in human plasma is described. The method is based on the inhibition of purified tissue plasminogen activator (tPA) by plasma diluted with a PAI-depleted plasma and the subsequent measurement of res...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(89)90250-8
更新日期:1989-11-01 00:00:00
abstract:BACKGROUND:Upon platelet stimulation with agonists, reactive oxygen species (ROS) generation enhances platelet activation and granule release. Whether ROS generation during platelet storage could be directly correlated with the expression of proinflammatory molecules and granule release has been investigated in this st...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2017.06.016
更新日期:2017-08-01 00:00:00
abstract:INTRODUCTION:To test the hypothesis that the platelet microparticle (PMP) and endothelial microparticle (EMP) may contribute to the hypercoagulability associated with microvascular thrombosis in patients with nontraumatc osteonecrosis of the femoral head (ONFH). MATERIALS AND METHODS:The study comprised 46 patients wh...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2008.04.001
更新日期:2008-01-01 00:00:00
abstract::Selective serotonin reuptake inhibitors (SSRIs) have been reported to reduce platelet aggregation induced by ADP. ADP induces platelet aggregation through two purinergic receptors P2Y1 and P2Y12. We characterized the inhibitory properties of SSRIs on ADP-induced platelet aggregation and investigated the effects of SSR...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2013.02.007
更新日期:2013-04-01 00:00:00
abstract::Heparin is an effective, relatively safe, inexpensive parenteral antithrombotic agent widely used in the prevention and treatment of thromboembolic disorders, but it has several limitations such as the marked intra- and inter-patient variability in its anticoagulant response, its poor bioavailability at low doses and ...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/j.thromres.2011.11.008
更新日期:2012-03-01 00:00:00
abstract::Upon incubation for 24 hours with [3H]arachidonic acid (AA, 1 mu Ci/ml), cultured endothelial cells from human umbilical vein incorporated one half of the added radioactivity, mostly into phospholipids (83% of the total cell radioactivity). Distribution of the label between the various phospholipid classes was found t...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(84)90068-9
更新日期:1984-04-15 00:00:00
abstract:INTRODUCTION:Nitric oxide (NO) plays an important role in the regulation of the cardiovascular system. It is produced by endothelial nitric oxide synthase (eNOS), which exhibits genetic polymorphisms. Although the clinically relevant polymorphism T(+IhI-786)C reduces eNOS-promoter activity, it is not clear whether circ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2004.09.003
更新日期:2005-01-01 00:00:00
abstract::Washed human platelets were incubated with commercial factor VIII concentrate, or with purified factor VIII coagulant moiety. Platelets were then washed again and lysed by sonication. VIII:Ag and vWf:Ag were measured in the platelet lysate prior to and after incubation of the lysate with phospholipase C (PL-C). Platel...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(87)90223-4
更新日期:1987-02-15 00:00:00
abstract::The effect of warfarin-induced factor VII deficiency on the skin bleeding time was investigated in 10 consecutive patients with mean prothrombin times of 33 sec and factor VII levels of 7%. Bleeding times in all but one subject were entirely within the normal range. We conclude that warfarin administration, even to th...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(83)90243-8
更新日期:1983-02-15 00:00:00
abstract::Evidence is presented for in vitro high affinity binding of serotonin (5-HT) by beta-thromboglobulin (beta TG) and platelet factor 4 (PF4) from human blood. Results include: 1) identification by radioimmunoassay of PF4 in specifically bound material obtained by 5-HT affinity chromatography of human platelet extracts; ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(83)90044-0
更新日期:1983-02-01 00:00:00
abstract:BACKGROUND:Minimizing bleeding and transfusion is desirable given its cost, complexity and potential for adverse events. Concerns have been heightened by recent data demonstrating that bleeding events may predict worse outcomes and by warnings about the safety of erythropoietic stimulating agents. Prior small studies s...
journal_title:Thrombosis research
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.thromres.2008.09.015
更新日期:2009-03-01 00:00:00
abstract:BACKGROUND:Rate of thalidomide-related VTE and risk factors in Japanese myeloma patients are not clear and effects of thromboprophylaxis remain controvertial. PATIENTS AND METHODS:We retrospectively analyzed a cohort data of registered Japanese myeloma patients treated with thalidomide-based regimens between 2009 and ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2012.11.014
更新日期:2013-02-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to investigate the effect of CYP2C19 polymorphism and co-therapy with esomeprazole on the antiplatelet efficacy of clopidogrel. BACKGROUND:The antiplatelet efficacy of clopidogrel depends on CYP2C19 polymorphism or the co-administration of some kind of proton pump inhibitor (PPI). ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2015.03.033
更新日期:2015-06-01 00:00:00
abstract::It has previously been observed that aspirin diminishes the increase in blood fibrinolytic activity during arm venous occlusion. We studied the duration of this inhibitory effect on fibrinolytic response during 20 minutes arm venous occlusion and its effect on fibrinolytic response during acute physical activity (stan...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(87)90282-9
更新日期:1987-04-15 00:00:00
abstract:INTRODUCTION:Retinal vein occlusion is a major cause of ocular morbidity. The precise mechanism leading to thrombosis in retinal vein occlusion has not yet been clearly elucidated. Several risk factors have been identified, including hypertension diabetes, history of cardiovascular disease, hypercholesterolemia, hyperh...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2009.07.010
更新日期:2009-12-01 00:00:00
abstract::SK, t-PA or APSAC were incubated in human plasma (adjusted to 300,000 platelets/mm3), in vitro, for up to 90 minutes using concentrations which were equivalent to those achieved in the treatment of AMI patients. Aggregation was measured in response to ADP and collagen. SK inhibited platelet aggregation after a 60 minu...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(90)90105-l
更新日期:1990-11-15 00:00:00