Abstract:
:Aims: The study was carried out to synthesise and characterise the chitosan-encapsulated genistein (CHI-En/Gen) and determine its anti-cancer and anti-angiogenic properties.Methods: The cytotoxic and anti-angiogenic activity of CHI-En/Gen was performed using MTT and chorioallantoic membrane assay. The molecular action was determined using flow cytometry and gene expression.Results: The synthesised CHI-En/Gen was in submicron size, spherical in shape and with entrapment efficiency and loading efficiency of 76.8% (w/w) and 32.6% (w/w), respectively. The CHI-En/Gen notably inhibited the growth and proliferation of human colorectal cancer cells (HT-29) while did not affect the viability of human dermal fibroblast as normal cell. The flow cytometry and the caspase-3 gene expression analyses revealed the apoptotic cells death in the HT-29 cells. Moreover, the encapsulated genistein showed anti-angiogenic activity.Conclusion: The CHI-En/Gen appeared as a promising carrier for the colon delivery of genistein to be used in complementary health approaches for the cancer prevention.
journal_name
J Microencapsuljournal_title
Journal of microencapsulationauthors
Rahmani F,Karimi E,Oskoueian Edoi
10.1080/02652048.2020.1740804subject
Has Abstractpub_date
2020-06-01 00:00:00pages
305-313issue
4eissn
0265-2048issn
1464-5246journal_volume
37pub_type
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
pub_type: 杂志文章,评审
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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