Abstract:
BACKGROUND:Because CD4CD25Foxp3 regulatory T cells (Tregs) are essential for the maintenance of self-tolerance, significant interest surrounds the developmental cues for thymic-derived natural Tregs (nTregs) and periphery-generated adaptive Tregs (aTregs). In the transplant setting, the allograft may play a role in the generation of alloantigen-specific Tregs, but this role remains undefined. We examined whether the immune response to a transplant allograft results in the peripheral generation of aTregs. METHODS:To identify generation of aTregs, purified graft-reactive CD4CD25 T cells were adoptively transferred to mice-bearing skin allograft. To demonstrate that aTregs are necessary for tolerance, DBA/2 skin was transplanted onto C57BL/6-RAG-1-deficient recipients adoptively transferred with purified sorted CD4CD25 T cells; half of the recipients undergo tolerance induction treatment. RESULTS:By tracking adoptively transferred cells, we show that purified graft-reactive CD4CD25 T lymphocytes up-regulate Foxp3 in mice receiving skin allografts in the absence of any treatment. Interestingly, cotransfer of antigen-specific nTregs suppresses the up-regulation of Foxp3 by inhibiting the proliferation of allograft-responsive T cells. In vitro data are consistent with our in vivo data-Foxp3 cells are generated on antigen activation, and this generation is suppressed on coculture with antigen-specific nTregs. Finally, blocking aTreg generation in grafted, rapamycin-treated mice disrupts alloantigen-specific tolerance induction. In contrast, blocking aTreg generation in grafted mice treated with nondepleting anti-CD4 plus anti-CD40L antibodies does not disrupt graft tolerance. CONCLUSIONS:We conclude that graft alloantigen stimulates the de novo generation of aTregs, and this generation may represent a necessary step in some but not all protocols of tolerance induction.
journal_name
Transplantationjournal_title
Transplantationauthors
Kim JI,O'connor MR,Duff PE,Zhao G,Lee KM,Eliades P,Deng S,Yeh H,Caton AJ,Markmann JFdoi
10.1097/TP.0b013e31820e50b3subject
Has Abstractpub_date
2011-04-15 00:00:00pages
707-13issue
7eissn
0041-1337issn
1534-6080journal_volume
91pub_type
杂志文章相关文献
TRANSPLANTATION文献大全abstract::To investigate whether graft-versus-host reactivity (GVHR) and the graft-versus-leukemia (GVL) effect can be differentiated, C3H-->AKR mixed bone marrow (BM) chimeras were prepared using two different conditioning regimens. Total body irradiation (TBI) chimeras were induced by infusing 5 x 1O(6) T cell depleted syngen...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199603150-00025
更新日期:1996-03-15 00:00:00
abstract:BACKGROUND:One of the alternative options to simultaneous pancreas-kidney transplantation (SPKT) for type I diabetics with renal failure is sequential transplant of a living donor kidney followed by a deceased donor pancreas transplant (pancreas after living donor kidney transplant [PALK]). We retrospectively compared ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3181dd3587
更新日期:2010-06-27 00:00:00
abstract::We have investigated retrospectively sera from 70 renal allograft recipients for the presence of antibodies binding to a preanastomosis biopsy of the donor kidney by an indirect immunoperoxidase technique. All recipients had a negative T cell lymphocytotoxic crossmatch. A positive immunoperoxidase crossmatch for kidne...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198812000-00011
更新日期:1988-12-01 00:00:00
abstract::The frequencies of circulating donor-reactive cytotoxic lymphocyte precursors (CLP) and Il-2-producing helper lymphocyte precursors (HLP) were determined by limiting dilution analysis in 19 kidney allograft recipients before and at various intervals (up to 2 years) after transplantation. A significant, selective, and ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199309000-00025
更新日期:1993-09-01 00:00:00
abstract::P-31 nuclear magnetic resonance (NMR) spectroscopy was used to evaluate heterotropic cardiac allograft rejection in the rat. To enable optimal application of NMR spectroscopy for the in vivo study of myocardial metabolism in the transplanted organ, an accessory heart transplantation to groin in the rat was used. The a...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198708000-00002
更新日期:1987-08-01 00:00:00
abstract::Experimental studies of vein homografts in rats have shown prolonged patency resulting from an attenuated immune response. This study examined the response to Golden Syrian hamster vein xenografts. While one group of Sprague-Dawley rats served as a control, a second group was sensitized with 5 X 10(6) viable hamster s...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198511000-00011
更新日期:1985-11-01 00:00:00
abstract:BACKGROUND:In transplantation, novel methods are required to augment the supply of donor organs. We report the first domino liver transplant in which a patient with familial amyloid polyneuropathy (FAP) received an orthotopic split liver graft, and her explanted liver was donated to another patient. Three successful li...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199806150-00016
更新日期:1998-06-15 00:00:00
abstract::Antibody-mediated injury is a major cause of allograft dysfunction and loss. Antibodies to ABH(O) blood group antigens are classic mediators of ABO-incompatible graft rejection, whereas donor-specific anti-HLA antibodies and, more recently, autoantibodies are appreciated as important contributors to allograft inflamma...
journal_title:Transplantation
pub_type: 杂志文章,评审
doi:10.1097/TP.0000000000001872
更新日期:2018-02-01 00:00:00
abstract::Acute antibody-mediated rejection (AAR) of mouse skin allografts was studied in nine donor-recipient combinations with increasing histoincompatibility ranging from an H-Y to a complete H-2 plus on-H-2 disparity. AAR was induced by the injection of specific alloantiserum along with a heterologous complement on day 7 af...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-197709000-00002
更新日期:1977-09-01 00:00:00
abstract::Surgical site infection (SSI) is an important cause of morbidity after orthotopic liver transplantation (OLT). Perioperative antibiotic prophylaxis is one of the main modifiable risk factors. We prospectively investigated the epidemiology, risk factors, and prognosis of SSI in a cohort of 167 OLT. Two different schedu...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3181735407
更新日期:2008-06-27 00:00:00
abstract:BACKGROUND:A pancreas transplant alone (PTA) in a nonuremic patient with brittle diabetes mellitus remains a rare procedure because the tradeoff for insulin independence is lifelong immunosuppression. METHODS:Herein we report our results at the University of Minnesota of 513 PTAs from December 17, 1966, through Decemb...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000296820.46978.3f
更新日期:2008-01-15 00:00:00
abstract:BACKGROUND:In patients with acute liver failure (ALF) of unknown cause, viral infections are believed to be involved. This study investigates the involvement of human herpesvirus-6 (HHV-6). METHODS:Thirty-two patients with ALF who underwent transplantations during a 6-year period were studied for viruses in biopsies f...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.TP.0000069233.13409.DF
更新日期:2003-08-15 00:00:00
abstract::Mus caroli blastocysts transferred to pseudopregnant Mus musculus implant but die about midgestation. Death of the M caroli embryos is associated with infiltration of the fetoplacental units by M musculus lymphocytes, including functional cytotoxic T lymphocytes (CTL). To determine if specific maternal antifetal immun...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198806000-00021
更新日期:1988-06-01 00:00:00
abstract::In order to study further whether a relationship exists between the extent of ischemia-preservation-reperfusion injury (IPRI) and acute rejection (AR) events in liver allografts, we retrospectively reviewed 213 consecutive cyclosporine-treated patients who received their first liver allograft between 1/1/93 and 12/31/...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199509270-00006
更新日期:1995-09-27 00:00:00
abstract:BACKGROUND:In Th (T helper) 1/Th2 balance in response to signals given during donor antigen presentation, induction of allograft prolongation is more often related to Th2-type than with Th1-type immunity. Here, we examined the effect of interleukin (IL)-33, a novel member of the IL-1 family, on cardiac allograft surviv...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3181d720af
更新日期:2010-05-27 00:00:00
abstract:BACKGROUND:The administration of systemic immunosuppressive agents to recipients of solid organ or bone marrow transplants results in an immunocompromised status. As the number of organ recipients and their life span increase with recent progress in organ transplantation, ocular complications tend to become more divers...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000259386.59375.8a
更新日期:2007-03-27 00:00:00
abstract:BACKGROUND:BK polyomavirus infection has been reported in 10% to 60% of renal transplant recipients with progression to BK nephropathy (BKN) occurring in 1% to 5% of patients. Graft loss occurs in up to 60% of renal transplant recipients with BKN. Because BK polyomavirus infection is believed, in part, to be a manifest...
journal_title:Transplantation
pub_type: 杂志文章,随机对照试验
doi:10.1097/TP.0b013e3181f2c94b
更新日期:2010-10-27 00:00:00
abstract:BACKGROUND:A previous donor intervention trial found that induction of mild therapeutic hypothermia in the brain-dead donor reduced the dialysis requirement after kidney transplantation. Consequences on the performance of cardiac allografts after transplantation were not explored to date. METHODS:Cohort study investig...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000002337
更新日期:2018-11-01 00:00:00
abstract:BACKGROUND:Controversy exists about the conditions effecting the development of forkhead/winghead helix transcription factor P3 (FOXP3) expressing T cells and their relevance in transplant recipients. METHODS:We generated carboxy-fluorescein diacetate succinimidyl ester-labeled CD4+CD25 high FOXP3+ cells in mixed lymp...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3181bbee98
更新日期:2009-12-15 00:00:00
abstract::Ling Zhi-8 (LZ-8) is a protein derived from the fungus Ganoderma lucidum and has immunomodulatory capacities. It was shown to be mitogenic toward mouse splenocytes in vitro and immunosuppressive in vivo by reducing antigen-induced antibody formation and by preventing completely the incidence of autoimmune diabetes in ...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:1995-09-15 00:00:00
abstract::A worldwide shortage of cadaveric donors has led to the increased utilization of elderly living donors, with controversial results. In an attempt to assess the effect of donor age on graft survival and subsequent renal function, we analyzed our clinical results in 276 consecutive recipients of living related renal tra...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199102000-00005
更新日期:1991-02-01 00:00:00
abstract:BACKGROUND:Small-for-size grafts often cause persistent conjugated hyperbilirubinemia in the recipient after adult-to-adult living donor liver transplantation, but the cause has not yet been clarified. In physiologic status, bilirubin is excreted from hepatocytes to the bile canaliculus by means of multidrug resistance...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.TP.0000089234.93366.6D
更新日期:2004-01-15 00:00:00
abstract::Methods are described for preparing large amounts of horse anti-human thymocyte globulin (ATG, ATGAM; The Upjohn Company) for clinical use. These methods have been used since 1968 to provide material for clinical trials. Characteristics of 40 lots of ATG are summarized. ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-197910000-00007
更新日期:1979-10-01 00:00:00
abstract::The incidence of interstitial pneumonitis (IP) was reviewed in 70 consecutive patients who received autologous marrow transplants for hematologic malignancies. All patients were treated with total-body irradiation (TBI), with or without other chemotherapeutic agents. Seven patients (10%) developed IP, 3 were due to cy...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198611000-00015
更新日期:1986-11-01 00:00:00
abstract:BACKGROUND:Thymoglobulin given before allo-hematopoietic stem-cell transplantation (HSCT) from unrelated donors reduces acute graft-versus-host disease (GvHD), but the optimal dose is unknown. METHOD:Four different doses of Thymoglobulin were given to 162 patients with hematologic malignancies undergoing unrelated don...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:2004-07-15 00:00:00
abstract:BACKGROUND:Hepatitis C virus (HCV) infection is common in kidney transplantation and is known to affect long-term patient and graft survival, as is time in renal-replacement therapy (RRT). The aim of this study was to investigate HCV in relation to time in RRT and its impact on outcome after transplantation. METHODS:A...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000131948.29742.24
更新日期:2004-09-15 00:00:00
abstract::To investigate the clinical manifestations of Aspergillus infections in lung transplant recipients, we reviewed the mycology and autopsy reports of all double (DLT=93) and single (SLT=48) lung transplant recipients from November 1983 to May 1993. Positive Aspergillus cultures were identified in 22% of the recipients (...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199603270-00013
更新日期:1996-03-27 00:00:00
abstract:BACKGROUND:There is an increasing appreciation of the deleterious effects of antibody and B cells on acute and chronic transplant outcomes. Many effector functions of antibody are mediated by a family of receptors (FcγRs) that are expressed on most immune cells, including neutrophils, natural killer cells, and B cells....
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000000287
更新日期:2014-08-15 00:00:00
abstract:BACKGROUND:Oral ingestion of potassium dichromate produces a complex spectrum of complications. It has an extremely poor prognosis and usually leads to rapid death. METHODS:We report the case of a 16-year-old male patient who was admitted to hospital after oral ingestion of potassium dichromate with suicidal intention...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200006150-00044
更新日期:2000-06-15 00:00:00
abstract::Liver transplantation is currently the only established treatment of end-stage liver disease, but it is limited by a severe shortage of viable donor livers. Donors after cardiac death (DCD) are an untapped source that could significantly increase the pool of available livers. Preservation of these DCD livers by conven...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e318192df6b
更新日期:2009-01-27 00:00:00