Recovery of warm ischemic rat liver grafts by normothermic extracorporeal perfusion.


:Liver transplantation is currently the only established treatment of end-stage liver disease, but it is limited by a severe shortage of viable donor livers. Donors after cardiac death (DCD) are an untapped source that could significantly increase the pool of available livers. Preservation of these DCD livers by conventional static cold storage (SCS) is associated with an unacceptable risk of primary nonfunction and delayed graft failure. Normothermic extracorporeal liver perfusion (NELP) has been suggested as an improvement over SCS. Livers recovered from male Lewis rats were subjected to 1 hr of warm ischemia and preserved with 5 hr of SCS or NELP, and transplanted into syngeneic recipients. As additional controls, non-ischemic livers preserved with 6 hr of SCS or NELP and unpreserved ischemic livers were transplanted. After NELP, ischemically damaged livers could be orthotopically transplanted into syngeneic recipients with 92% survival (n=13) after 4 weeks, which was comparable with control animals that received healthy livers preserved by SCS (n=9) or NELP (n=11) for 6 hr. On the other hand, animals from ischemia/SCS control group all died within 12 hr postoperatively (n=6). Similarly, animals that received ischemic livers without preservation all died within 24 hr after transplantation (n=6). These results suggest that NELP has the potential to reclaim warm ischemic livers that would not be transplantable otherwise. The rat model in this study is a useful platform to further optimize NELP as a method of recovery and preservation of DCD livers.






Tolboom H,Pouw RE,Izamis ML,Milwid JM,Sharma N,Soto-Gutierrez A,Nahmias Y,Uygun K,Berthiaume F,Yarmush ML




Has Abstract


2009-01-27 00:00:00














  • Bone mineral density changes within six months of renal transplantation.

    abstract:BACKGROUND:The effective use of new steroid-sparing immunosuppressive regimens may lower cumulative glucocorticoid use among renal transplant recipients. However, it is unknown what effect this therapeutic trend has had on bone disease. METHODS:Unselected newly transplanted inpatients (n=45) were identified and compre...


    pub_type: 杂志文章


    authors: Mikuls TR,Julian BA,Bartolucci A,Saag KG

    更新日期:2003-01-15 00:00:00

  • Differential regulation of the nuclear factor-κB pathway by rabbit antithymocyte globulins in kidney transplantation.

    abstract:BACKGROUND:Induction therapy is associated with excellent short-term kidney graft outcome. The aim of this study was to evaluate differences in the intragraft transcriptome after successful induction therapy using two rabbit antithymocyte globulins. METHODS:The expression of 376 target genes involved in tolerance, inf...


    pub_type: 杂志文章


    authors: Urbanova M,Brabcova I,Girmanova E,Zelezny F,Viklicky O

    更新日期:2012-03-27 00:00:00

  • Pubertal Development in Pediatric Kidney Transplant Patients Receiving Mammalian Target of Rapamycin Inhibitors or Conventional Immunosuppression.

    abstract:BACKGROUND:Data regarding the onset of puberty in children receiving mammalian target of rapamycin (mTOR) inhibitors are limited. METHODS:Kidney transplant patients aged <14 years were analyzed retrospectively to a maximum age of 18 years, with a minimal observation period of 1 year. Immunosuppression comprised (1) st...


    pub_type: 杂志文章


    authors: Förster J,Ahlenstiel-Grunow T,Zapf A,Mynarek M,Pape L

    更新日期:2016-11-01 00:00:00

  • Tacrolimus in cardiac transplantation: efficacy and safety of a novel dosing protocol.

    abstract:BACKGROUND:Although used for more than 20 years, optimal dosing strategies of most immunosuppressants have never been determined. Tacrolimus, one of the newer agents used in solid-organ transplantation, is gaining increasing popularity because of its ability to reverse refractory rejection in cyclosporine-treated patie...


    pub_type: 临床试验,杂志文章


    authors: Baran DA,Galin I,Sandler D,Segura L,Cheng J,Courtney MC,Correa R,Chan M,Fallon JT,Spielvogel D,Lansman SL,Gass AL

    更新日期:2002-10-27 00:00:00

  • Immunosuppression with FTY720 and cyclosporine A inhibits rejection of adult porcine islet xenografts in rats.

    abstract:BACKGROUND:Our aim was to evaluate the effect of FTY720 in discordant islet xenotransplantation. METHODS:Fetal porcine islet-like cell clusters (ICCs) were transplanted into normoglycemic rats that were either left untreated or treated with FTY720 only, with FTY720 plus cyclosporine A (CsA) or with CsA only. Twelve or...


    pub_type: 杂志文章


    authors: Maeda A,Goto M,Zhang J,Bennet W,Groth CG,Korsgren O,Wennberg L

    更新日期:2003-04-27 00:00:00

  • Use of the methylxanthine derivative A802715 in transplantation immunology: I. Strong in vitro inhibitory effects on CD28-costimulated T cell activities.

    abstract:BACKGROUND:Recently, methylxanthines such as pentoxifylline (PTX) were shown to be immunosuppressive in vitro. Unfortunately, when used in transplant patients, PTX was poorly active as an immunosuppressant. Here we report that the new methylxanthine derivative A802715 not only is more active than PTX, it also suppresse...


    pub_type: 杂志文章


    authors: Lin Y,Goebels J,Rutgeerts O,Kasran A,Van Gool S,Ceuppens J,Schönharting M,Waer M

    更新日期:1997-06-27 00:00:00

  • Tacrolimus trough level at discharge predicts acute rejection in moderately sensitized renal transplant recipients.

    abstract:BACKGROUND:Goal tacrolimus concentrations for the prevention of rejection in sensitized renal transplant recipients are not well established. METHODS:We evaluated the association between discharge tacrolimus trough concentration and the incidence of biopsy-proven acute rejection (BPAR) in 216 moderately sensitized ren...


    pub_type: 杂志文章


    authors: Richards KR,Hager D,Muth B,Astor BC,Kaufman D,Djamali A

    更新日期:2014-05-27 00:00:00

  • Proton nuclear magnetic resonance analysis of hepatic bile from donors and recipients in human liver transplantation.

    abstract::The current shortage of donor organs in liver transplantation has led experienced transplant centers to use more "marginal" grafts. The development of a reliable technique of bile collection gives access to hepatic bile from donors and recipients for bile analysis to characterize the grafts. Proton nuclear magnetic re...


    pub_type: 杂志文章


    authors: Melendez HV,Ahmadi D,Parkes HG,Rela M,Murphy G,Heaton N

    更新日期:2001-09-15 00:00:00

  • Renal transplant from very old donors: how far can we go?

    abstract:BACKGROUND:The organ shortage has led many transplant centers to accept kidneys from old, suboptimal deceased donors, and make increasing use of old-for-old allocation systems. We report the experience of an Italian transplant center in the utilization of "ultra-old" (>75 years old) donors. METHODS:Sixty grafts from d...


    pub_type: 杂志文章


    authors: Collini A,Kalmar P,Dhamo A,Ruggieri G,Carmellini M

    更新日期:2009-06-27 00:00:00

  • The vascular endothelial cell antigen system.

    abstract::Vascular endothelial cells (VEC) are clearly immunogenic and express antigens unique to vascular endothelial cells. The observation that peripheral blood monocytes also express this VEC antigen system made prospective testing feasible. Preformed antibody to the VEC/monocyte antigen system of the donor usually leads to...


    pub_type: 杂志文章


    authors: Cerilli J,Brasile L,Galouzis T,Lempert N,Clarke J

    更新日期:1985-03-01 00:00:00

  • Fine-needle aspiration biopsy in the monitoring of liver allografts. Different cellular findings during rejection and cytomegalovirus infection.

    abstract::Fine-needle aspiration biopsies were used for clinical monitoring of liver allografts; 21 patients with an inflammatory episode of acute rejection (12.0 +/- 3.3 CIU at the peak) were studied; all episodes were reversible. The inflammatory infiltrate consisted mainly of lymphoid cells, including lymphoid blasts, with i...


    pub_type: 杂志文章


    authors: Lautenschlager I,Höckerstedt K,Salmela K,Isoniemi H,Holmberg C,Jalanko H,Häyry P

    更新日期:1990-11-01 00:00:00

  • The development and specificity of antiidiotypic antibodies in renal transplant recipients receiving single-donor blood transfusions.

    abstract::Multiple pretransplant sera obtained from alloimmunized renal transplant recipients were tested for the presence of antiidiotypic-like antibodies (AB2) that inhibit donor-specific HLA antibodies in the microlymphocytotoxicity assay. Fourteen patients received repetitive single-donor blood transfusions (SDT). In this p...


    pub_type: 杂志文章


    authors: Phelan DL,Rodey GE,Anderson CB

    更新日期:1989-07-01 00:00:00

  • Association between capsule diameter, adequacy of encapsulation, and survival of microencapsulated rat islet allografts.

    abstract::As a consequence of its large volume, a microencapsulated islet graft can be implanted only into the peritoneal cavity. The graft volume can be reduced by using small capsules. However, reduction of the diameter of the capsules holds a certain risk, because with smaller capsules, more islets may be found to protrude f...


    pub_type: 杂志文章


    authors: De Vos P,De Haan B,Pater J,Van Schilfgaarde R

    更新日期:1996-10-15 00:00:00

  • Anti-CD20 monoclonal antibody (rituximab) therapy for acute cardiac humoral rejection: a case report.

    abstract::Humoral or antibody-mediated rejection in cardiac transplant recipients is mediated by donor-specific cytotoxic antibodies and is histologically defined by linear deposits of immunoglobulin and complement in the myocardial capillaries. Antibody-mediated rejection often is accompanied by hemodynamic compromise and is a...


    pub_type: 杂志文章


    authors: Aranda JM Jr,Scornik JC,Normann SJ,Lottenberg R,Schofield RS,Pauly DF,Miles M,Hill JA,Sleasman JW,Skoda-Smith S

    更新日期:2002-03-27 00:00:00

  • The effect of chronic graft-versus-host disease on B cell development.

    abstract::Chronic graft-versus-host disease often results in a combined deficiency of humoral and cell-mediated immunity. Clinical and experimental studies have suggested that the decrease in B cell responsiveness is due to a failure of B cell production in the bone marrow, intrinsic B cell defects, excessive suppressor T cell ...


    pub_type: 杂志文章


    authors: Schreiber KL,Forman J

    更新日期:1993-03-01 00:00:00

  • High Intrapatient Variability of Tacrolimus Exposure in the Early Period After Liver Transplantation Is Associated With Poorer Outcomes.

    abstract:BACKGROUND:Tacrolimus (TAC) is the cornerstone of immunosuppressive regimen in liver transplantation (LT). Its pharmacokinetics is characterized by a high interpatient and intrapatient variability (IPV) leading to an unpredictable dose-response relationship. The aim of our study was to evaluate the impact of TAC IPV (I...


    pub_type: 杂志文章


    authors: Rayar M,Tron C,Jézéquel C,Beaurepaire JM,Petitcollin A,Houssel-Debry P,Camus C,Verdier MC,Dehlawi A,Lakéhal M,Desfourneaux V,Meunier B,Sulpice L,Bellissant E,Boudjema K,Lemaitre F

    更新日期:2018-03-01 00:00:00

  • Twenty-four-hour preservation of the pig heart by a portable hypothermic perfusion system.

    abstract::A portable hypothermic perfusion system for storage of hearts has been developed. The system uses the airlift pump principle, whereby the flow of gas maintains circulation of the perfusate through the heart; no other energy source is required. Performance on ex vivo functional testing of 10 pig hearts stored for 20 to...


    pub_type: 杂志文章


    authors: Wicomb WN,Cooper DK,Barnard CN

    更新日期:1982-11-01 00:00:00

  • Requirement for help in the generation of alloantigen-specific helper cells.

    abstract::Control mechanisms that regulate the induction of T helper cells are a key component of conceptual schemes to understand the network of interactions controlling the immune response. In this work, using a sequential three-step culture system, we demonstrate that the generation of the T helper cells that participate in ...


    pub_type: 杂志文章


    authors: Pilarski LM,McKenzie IF

    更新日期:1985-09-01 00:00:00

  • Multivariate analysis of donor risk factors for graft survival in kidney transplantation.

    abstract:BACKGROUND:The results of the transplantation of marginal donor kidneys remain controversial. This study aimed to investigate the impact of donor risk factors as predictors of kidney-graft outcome. METHODS:Allograft failure risk factors were studied in 7,209 cadaveric kidney-transplant recipients reporting to the Etab...


    pub_type: 杂志文章


    authors: Pessione F,Cohen S,Durand D,Hourmant M,Kessler M,Legendre C,Mourad G,Noël C,Peraldi MN,Pouteil-Noble C,Tuppin P,Hiesse C

    更新日期:2003-02-15 00:00:00

  • Prevention of insulitis and diabetes in nonobese diabetic mice by administration of FK506.

    abstract::We investigated the preventive effect of the immunosuppressive agent FK506 on autoimmune insulitis in nonobese diabetic mice. The mice were given FK506 in a dose of 1.0 mg/kg, every other day, from age 2 to 12, 2 to 6, and 4 to 12 weeks, respectively; after which, the incidence of insulitis and overt diabetes was moni...


    pub_type: 杂志文章


    authors: Kai N,Motojima K,Tsunoda T,Kanematsu T

    更新日期:1993-04-01 00:00:00

  • Management of Patients Who Receive an Organ Transplant Abroad and Return Home for Follow-up Care: Recommendations From the Declaration of Istanbul Custodian Group.

    abstract::Eradicating transplant tourism depends on complex solutions that include efforts to progress towards self-sufficiency in transplantation. Meanwhile, professionals and authorities are faced with medical, legal, and ethical problems raised by patients who return home after receiving an organ transplant abroad, particula...


    pub_type: 杂志文章


    authors: Domínguez-Gil B,Danovitch G,Martin DE,López-Fraga M,Van Assche K,Morris ML,Lavee J,Erlich G,Fadhil R,Busic M,Rankin G,Al-Rukhaimi M,OʼConnell P,Chin J,Norman T,Massari P,Kamel R,Delmonico FL,Declaration of Istanbul Cu

    更新日期:2018-01-01 00:00:00

  • Mechanisms of acquired thymic unresponsiveness to renal allografts. Thymic recognition of immunodominant allo-MHC peptides induces peripheral T cell anergy.

    abstract::We have recently shown that a single intrathymic injection of synthetic 25mer peptides, representing full sequences of the hypervariable domain of RT1.BuB (4 peptides) and RT1.Du beta (4 peptides) WF class II MHC molecules, 48 hr before transplantation induces donor-specific unresponsiveness to WF rat renal allografts...


    pub_type: 杂志文章


    authors: Sayegh MH,Perico N,Gallon L,Imberti O,Hancock WW,Remuzzi G,Carpenter CB

    更新日期:1994-07-27 00:00:00

  • Liver Transplantation for NASH-Related Hepatocellular Carcinoma Versus Non-NASH Etiologies of Hepatocellular Carcinoma.

    abstract:BACKGROUND:Liver transplant (LT) for nonalcoholic steatohepatitis (NASH) related hepatocellular carcinoma (HCC) is not well characterized in the literature. The aim of the study was to examine characteristics and outcomes of patients who had LT for NASH-HCC (NASH) versus HCC from other liver diseases (non-NASH). METHO...


    pub_type: 杂志文章,多中心研究


    authors: Sadler EM,Mehta N,Bhat M,Ghanekar A,Greig PD,Grant DR,Yao F,Sapisochin G

    更新日期:2018-04-01 00:00:00

  • Increased expression of endoarterial vascular cell adhesion molecule-1 mRNA in an experimental model of lung transplant rejection: diagnosis by pulmonary arterial biopsy.

    abstract:BACKGROUND:Early detection of rejection after lung transplantation may prevent allograft failure. This study determines if mRNA from the cell adhesion molecules intercellular adhesion molecule-1, vascular cell adhesion molecule (VCAM)-1, and E-selectin in pulmonary endovascular tissue samples could be markers of early ...


    pub_type: 杂志文章


    authors: Rothman A,Mann D,Behling CA,McGraw M,Seslar S,Shiu P,Zhang L,Kriett JM

    更新日期:2003-04-15 00:00:00

  • Toxicity of rapamycin--a comparative and combination study with cyclosporine at immunotherapeutic dosage in the rat.

    abstract::Sprague-Dawley rats were treated for 14 days with rapamycin (RAP; 1.5 mg/kg/day i.p.), cyclosporine (15 mg/kg/day by gavage), both drugs in combination, or appropriate drug vehicles. Hematological parameters and biochemical indices of renal and hepatic function were determined throughout the experimental period, at th...


    pub_type: 杂志文章


    authors: Whiting PH,Woo J,Adam BJ,Hasan NU,Davidson RJ,Thomson AW

    更新日期:1991-08-01 00:00:00

  • In vivo bioluminescence imaging of transplanted islets and early detection of graft rejection.

    abstract:BACKGROUND:Bioluminescence imaging (BLI) modalities are being developed to monitor islet transplant mass and function in vivo. The aim of this study was to use the BLI system to determine how the change in functional islet mass correlated to metabolic abnormalities during the course of alloimmune rejection in a murine ...


    pub_type: 杂志文章


    authors: Chen X,Zhang X,Larson CS,Baker MS,Kaufman DB

    更新日期:2006-05-27 00:00:00

  • Transient complement inhibition plus T-cell immunosuppression induces long-term survival of mouse-to-rat cardiac xenografts.

    abstract:BACKGROUND:The use of anti-B-cell and T-cell immunosuppressive agents leads to only a few weeks' survival of mouse-to-rat cardiac xenografts. METHODS:BALB/c cardiac xenografts were transplanted to Lewis rats treated with cyclosporine (CsA) and/or cobra venom factor (CVF). RESULTS:CsA alone did not prolong xenograft s...


    pub_type: 杂志文章


    authors: Koyamada N,Miyatake T,Candinas D,Mark W,Hechenleitner P,Hancock WW,Soares MP,Bach FH

    更新日期:1998-05-15 00:00:00

  • A Scandinavian two-center study of BMA 031 in steroid-resistant rejection of renal grafts.

    abstract::BMA 031 (Behring Monoclonal Antibody) was given to 25 renal graft recipients with biopsy-proven steroid-resistant rejections. A dose of 50 mg of BMA 031 was given i.v. on 7 consecutive days concomitantly with a standard triple-drug regimen. No premedication was administered before the first BMA 031 dose. After the fir...


    pub_type: 临床试验,杂志文章,多中心研究


    authors: Pfeffer PF,Ohlman S,Jakobsen A,Fauchald P,Leivestad T,Tydén G,Flatmark A

    更新日期:1993-08-01 00:00:00

  • A partial conditioning approach to achieve mixed chimerism in the rat: depletion of host natural killer cells significantly reduces the amount of total body irradiation required for engraftment.

    abstract:BACKGROUND:Mixed allogeneic bone marrow chimerism induces tolerance to solid organ grafts. Although we previously reported that partially ablative conditioning with 700 cGy of total body irradiation (TBI) is sufficient to allow for bone marrow engraftment in mice, we determined that a minimum of 1000 cGy was required i...


    pub_type: 杂志文章


    authors: Neipp M,Gammie JS,Exner BG,Li S,Chambers WH,Pham SM,Ildstad ST

    更新日期:1999-08-15 00:00:00

  • Alterations in chemokine receptor CCR5 expression on blood dendritic cells correlate with acute graft-versus-host disease.

    abstract:BACKGROUND:Dendritic cells (DC) are important in the development of acute graft-versus-host disease (GVHD) after allogeneic hemopoietic cell transplantation (alloHCT). The trafficking of immature DC from blood to GVHD target organs is likely to be regulated by chemokine receptors. METHODS:We performed flow cytometry t...


    pub_type: 杂志文章


    authors: Shahin K,Sartor M,Hart DN,Bradstock KF

    更新日期:2013-10-27 00:00:00