Recovery of warm ischemic rat liver grafts by normothermic extracorporeal perfusion.


:Liver transplantation is currently the only established treatment of end-stage liver disease, but it is limited by a severe shortage of viable donor livers. Donors after cardiac death (DCD) are an untapped source that could significantly increase the pool of available livers. Preservation of these DCD livers by conventional static cold storage (SCS) is associated with an unacceptable risk of primary nonfunction and delayed graft failure. Normothermic extracorporeal liver perfusion (NELP) has been suggested as an improvement over SCS. Livers recovered from male Lewis rats were subjected to 1 hr of warm ischemia and preserved with 5 hr of SCS or NELP, and transplanted into syngeneic recipients. As additional controls, non-ischemic livers preserved with 6 hr of SCS or NELP and unpreserved ischemic livers were transplanted. After NELP, ischemically damaged livers could be orthotopically transplanted into syngeneic recipients with 92% survival (n=13) after 4 weeks, which was comparable with control animals that received healthy livers preserved by SCS (n=9) or NELP (n=11) for 6 hr. On the other hand, animals from ischemia/SCS control group all died within 12 hr postoperatively (n=6). Similarly, animals that received ischemic livers without preservation all died within 24 hr after transplantation (n=6). These results suggest that NELP has the potential to reclaim warm ischemic livers that would not be transplantable otherwise. The rat model in this study is a useful platform to further optimize NELP as a method of recovery and preservation of DCD livers.






Tolboom H,Pouw RE,Izamis ML,Milwid JM,Sharma N,Soto-Gutierrez A,Nahmias Y,Uygun K,Berthiaume F,Yarmush ML




Has Abstract


2009-01-27 00:00:00














  • Murine sera cytotoxicity toward human B cells and their effect on human mixed lymphocyte reactions.

    abstract::Seven murine anti-H-2 and three nonimmune mouse sera were tested for cytotoxicity toward B and T lymphocytes from a panel of human donors. One group of sera, including two anti-H-2.33 sera, exhibited cytotoxicity directed exclusively toward human B but not T cells from all donors. Absorption studies on human lymphobla...


    pub_type: 杂志文章


    authors: Verghese MW,Ward FE

    更新日期:1979-08-01 00:00:00

  • Functional evaluation of T helper, T suppressor, and B lymphocytes in lethally irradiated rhesus monkeys injected with autologous bone marrow.

    abstract::Lethally irradiated rhesus monkeys were treated with autologous bone marrow that had either been (1) nontreated, i.e., normal; (2) depleted of T lymphocytes with a monoclonal antibody directed against rhesus T lymphocytes; (3) fractionated with the soybean agglutinin (SBA- fraction); or (4) fractionated with SBA and f...


    pub_type: 杂志文章


    authors: Gengozian N,Moriarty C,Good RA

    更新日期:1992-06-01 00:00:00

  • Renal transplantation without sutures using the vascular clipping system for renal artery and vein anastomosis--a new technique.

    abstract::A cadaveric renal transplant was performed on a 63-year-old woman. The donor renal artery and vein were anastomosed to the recipient external iliac vessels using the vascular clipping system. These vascular anastomoses were performed with four stay sutures and several clips for each anastomosis, without a continuous v...


    pub_type: 杂志文章


    authors: Mital D,Foster PF,Jensik SC,del Rio JV,Sankary HN,McChesney LP,Williams JW

    更新日期:1996-10-27 00:00:00

  • A simple model to estimate survival after retransplantation of the liver.

    abstract::To formulate a model predicting survival after liver retransplantation, we analyzed in detail the last 150 cases of hepatic retransplantation at UCLA. Cox proportional hazards regression analysis identified five variables that demonstrated independent simultaneous prognostic value in estimating patient survival after ...


    pub_type: 杂志文章


    authors: Markmann JF,Gornbein J,Markowitz JS,Levy MF,Klintmalm GB,Yersiz H,Morrisey M,Drazan K,Farmer DG,Ghobrial RM,Goss J,Seu P,Martin P,Goldstein LI,Busuttil RW

    更新日期:1999-02-15 00:00:00

  • Long-term results of ABO-incompatible kidney transplantation with antigen-specific immunoadsorption and rituximab.

    abstract::ABO-incompatible (ABOi) kidney transplantation has gained a renewed interest during the past years. In 2001, a protocol for ABOi kidney transplantation based on antigen-specific immunoadsorption and rituximab was introduced at our center. In this study long-term graft function using this protocol was assessed. All ABO...


    pub_type: 杂志文章


    authors: Genberg H,Kumlien G,Wennberg L,Tydén G

    更新日期:2007-12-27 00:00:00

  • Predictors of graft survival in pediatric living-related kidney transplant recipients.

    abstract:BACKGROUND:A successful kidney transplant from a living-related donor (LRD) remains the most effective renal replacement therapy for children with end-stage renal failure. The use of LRD kidneys results in decreased time on dialysis, increased graft survival, and better function compared with kidneys transplanted from ...


    pub_type: 杂志文章


    authors: Ishitani M,Isaacs R,Norwood V,Nock S,Lobo P

    更新日期:2000-07-27 00:00:00

  • Tolerance induction in a fully allogeneic combination using anti-T cell receptor-alpha beta monoclonal antibody, low dose irradiation, and donor bone marrow transfusion.

    abstract::In a murine strain combination disparate in both H-2 antigens and minor histocompatibility antigens consisting of C57BL/6 (B6; H-2b, Mls-1b) mice as recipients and AKR/J (AKR; H-2k, Mls-1a) mice as donors, we reported that the administration of anti-TCR-alpha beta mAb nonspecifically suppresses the ability to reject a...


    pub_type: 杂志文章


    authors: Nomoto K,Yung-Yun K,Omoto K,Umesue M,Murakami Y,Matsuzaki G,Nomoto K

    更新日期:1995-02-15 00:00:00

  • De novo sirolimus-based immunosuppression after liver transplantation for hepatocellular carcinoma: long-term outcomes and side effects.

    abstract:BACKGROUND:We report long-term outcomes and side effects after transplantation for hepatocellular carcinoma (HCC) using de novo, sirolimus-based immunosuppression (IS). METHODS:A total of 70 patients with HCC (mean age: 54.4+/-7 years, female/male: 12/58) were transplanted and included in the study. Immunosuppression ...


    pub_type: 杂志文章


    authors: Toso C,Meeberg GA,Bigam DL,Oberholzer J,Shapiro AM,Gutfreund K,Ma MM,Mason AL,Wong WW,Bain VG,Kneteman NM

    更新日期:2007-05-15 00:00:00

  • Alloantibodies against donor epidermis and early kidney transplant rejection.

    abstract::We have previously observed that transplant recipient sera with endothelial antibodies are bound to epidermal cells, as shown by immunofluorescence on sections of skin. It was also reported that early kidney failures that occurred despite negative T cell crossmatches were associated with, and could have been predicted...


    pub_type: 杂志文章


    authors: Moraes JR,Moraes ME,Luo YM,Stastny P

    更新日期:1991-02-01 00:00:00

  • Alterations in rat pulmonary macrophage function by the immunosuppressive agents cyclosporine, azathioprine, and prednisolone.

    abstract::Disturbances of the immune response of the lung induced by the action of immunosuppressive agents on the functional abilities of rat pulmonary alveolar macrophages (PAM) were analyzed following in vitro incubation or in vivo administration (for 30 days) of cyclosporinea, (CsA) azathioprine (Az) or prednisolone (Pr). T...


    pub_type: 杂志文章


    authors: Drath DB,Kahan BD

    更新日期:1983-06-01 00:00:00

  • Pancreas retransplantation: a second chance for diabetic patients?

    abstract:BACKGROUND:If pancreas transplantation is a validated alternative for type 1 diabetic patients with end-stage renal disease, the management of patients who have lost their primary graft is poorly defined. This study aims at evaluating pancreas retransplantation outcome. METHODS:Between 1976 and 2008, 569 pancreas tran...


    pub_type: 杂志文章,多中心研究


    authors: Buron F,Thaunat O,Demuylder-Mischler S,Badet L,Brunet M,Ber CE,Thivolet C,Martin X,Berney T,Morelon E

    更新日期:2013-01-27 00:00:00

  • Immunological characteristics of purified pancreatic islet grafts.

    abstract::In a DBA/2 (H-2d) pancreatic islet-to-B6AF1 (H-2b/k.d) recipient combination, the graft survival of hand-picked islets was compared with that of "crude digested" islets that were prepared simply by collagenase digestion and Ficoll gradient separation and were contaminated with lymph nodes and vascular and ductal tissu...


    pub_type: 杂志文章


    authors: Gotoh M,Maki T,Satomi S,Porter J,Monaco AP

    更新日期:1986-10-01 00:00:00

  • CD4+ and B lymphocytes in transplantation immunity. II. Augmented rejection of tumor allografts by mice lacking B cells.

    abstract::To test the importance of B lymphocytes in immunity to major histocompatibility complex class I alloantigens, B cell-deficient mice were generated by reconstituting severe combined immunodeficiency mice, which lack functional B and T lymphocytes, with T cells or with both T and B cells. The reconstituted mice were cha...


    pub_type: 杂志文章


    authors: Monach PA,Schreiber H,Rowley DA

    更新日期:1993-06-01 00:00:00

  • Cyclosporine minimization and cost reduction in renal transplant recipients receiving a C2-monitored, cyclosporine-based quadruple immunosuppressive regimen.

    abstract:BACKGROUND:Targeting 2-hr postdose cyclosporine (C2) levels to 1,000 to 1,700 mg/dL during the first 6 months after renal transplantation is recommended for triple immunosuppressive regimens. This trial determines whether lower C2 levels could be targeted safely in de novo kidney transplant recipients under a quadruple...


    pub_type: 临床试验,杂志文章


    authors: Hardinger KL,Schnitzler MA,Koch MJ,Enkvetchakul D,Desai N,Jendrisak M,Lowell JA,Miller B,Shenoy S,Brennan DC

    更新日期:2004-10-27 00:00:00

  • The human "Treg MLR": immune monitoring for FOXP3+ T regulatory cell generation.

    abstract:BACKGROUND:Controversy exists about the conditions effecting the development of forkhead/winghead helix transcription factor P3 (FOXP3) expressing T cells and their relevance in transplant recipients. METHODS:We generated carboxy-fluorescein diacetate succinimidyl ester-labeled CD4+CD25 high FOXP3+ cells in mixed lymp...


    pub_type: 杂志文章


    authors: Levitsky J,Miller J,Leventhal J,Huang X,Flaa C,Wang E,Tambur A,Burt RK,Gallon L,Mathew JM

    更新日期:2009-12-15 00:00:00

  • Biochemical characterization of human vascular endothelial cell-monocyte antigens defined by monoclonal antibodies.

    abstract::Although the clinical relevance of endothelial cell-monocyte (E-M) antigens has been demonstrated in organ graft transplantation, very limited data exist describing the nature of these antigens. The current study presents biochemical characterization of three different surface antigens of endothelial cells and monocyt...


    pub_type: 杂志文章


    authors: Wood NL,Schook LB,Studer EJ,Mohanakumar T

    更新日期:1988-04-01 00:00:00

  • Fulminant hepatitis is significantly increased in hepatitis B carriers after allogeneic bone marrow transplantation.

    abstract:BACKGROUND:Bone marrow transplantation (BMT) is effective treatment for many hematologic disease, but performed in a population with a high endemic hepatitis B virus carrier rate, the incidence of liver function impairment and fulminant hepatitis (FH) is expected to be raised. METHODS:Forty-three hepatitis B virus car...


    pub_type: 杂志文章


    authors: Chen PM,Chiou TJ,Fan FS,Liu JM,Hsieh RK,Yen CC,Wang WS,Liu JH

    更新日期:1999-06-15 00:00:00

  • Vascular thymus transplantation in rats. Technique, morphology, and function.

    abstract::A new method of thymus transplantation is introduced, in which the graft is directly connected with the recipient's vascular system. This procedure was used both in euthymic rats and congenitally athymic nude rats. At all tested intervals after transplantation thymus grafts hardly differed from the recipient's own thy...


    pub_type: 杂志文章


    authors: Kampinga J,Schuurman HJ,Pol GH,Bartels H,Broekhuizen R,Vaessen LM,Tielen FJ,Rozing J,Blaauw EH,Roser B

    更新日期:1990-10-01 00:00:00

  • Successful surgical salvage of pancreas allograft.

    abstract:BACKGROUND:Early and late complications related to the pancreas after simultaneous kidney-pancreas transplantation (SKPT) frequently result in graft loss. The authors describe a surgical rescue technique that allows salvage of the pancreatic graft when surgical complications appear after the transplant. METHODS:Of 158...


    pub_type: 杂志文章


    authors: Orsenigo E,Cristallo M,Socci C,Castoldi R,Fiorina P,Invernizzi L,Caldara R,Secchi A,Di Carlo V

    更新日期:2003-01-27 00:00:00

  • Outcome of kidney transplant recipients with previous human herpesvirus-8 infection.

    abstract:BACKGROUND:The consequences of a prior human herpesvirus-8 (HHV-8) infection in kidney-transplant recipients are still partially unknown. The aim of this monocentric study was to determine the prevalence of HHV-8-seropositive patients at the time of transplantation and to identify the main clinical events of these HHV-...


    pub_type: 杂志文章


    authors: Francès C,Mouquet C,Marcelin AG,Barete S,Agher R,Charron D,Benalia H,Dupin N,Piette JC,Bitker MO,Calvez V

    更新日期:2000-05-15 00:00:00

  • Gastric aspiration for diagnosis of pulmonary tubercolosis in adult renal allograft recipients.

    abstract::Of 213 renal allgraft recipients suspected to have had pulmonary tuberculosis, 132 had sputum examinations and 14 showed acid-fast bacilli. Of the remaining 118 patients, 25 had gastric aspirations, 18 had bronchoalveolar lavage, and 75 did not require further investigation because of spontaneous improvement or confir...


    pub_type: 杂志文章


    authors: John GT,Juneja R,Mukundan U,Date A,Vincent L,Jacob CK,Shastry JC

    更新日期:1996-03-27 00:00:00

  • Rhesus monkey immature monocyte-derived dendritic cells generate alloantigen-specific regulatory T cells from circulating CD4+CD127-/lo T cells.

    abstract:BACKGROUND:Generation of non-human primate regulatory T cells (Treg) with alloantigen (alloAg) specificity would allow their testing in preclinical transplant models. Low recovery of Treg from peripheral blood limits their potential utility. In small animals and humans, conventional myeloid dendritic cells (DC) have be...


    pub_type: 杂志文章


    authors: Zahorchak AF,Raimondi G,Thomson AW

    更新日期:2009-11-15 00:00:00

  • Orthotopic renal transplantation in the rabbit.

    abstract::A technique of orthotopic renal transplantation in rabbits is described. The technique has been evolved in an experience of over 400 grafts, and difficulties encountered in producing satisfactory anaesthesia and techniques of anastomosis and in obtaining reliable graft function postoperatively have been defined and ov...


    pub_type: 杂志文章


    authors: Dunn DC

    更新日期:1976-11-01 00:00:00

  • Inhibition of cell-mediated microcytotoxicity and stimulation of mixed lymphocyte reactivity by mouse pregnancy serum.

    abstract::Blocking activity of mouse pregnancy serum was investigated using a well defined one-way mixed lymphocyte culture and a cell-mediated microcytotoxicity test. Allogeneic (C57BL (H-2b) X CBA/Ca (H-2k)) and syngeneic (C57BL (H-2b) X C57BL (H-2b)) pregnancy and postpartum sera were found to stimulate rather than inhibit t...


    pub_type: 杂志文章


    authors: Smith G

    更新日期:1978-11-01 00:00:00

  • P-31 nuclear magnetic resonance spectroscopic evaluation of heterotopic cardiac allograft rejection in the rat.

    abstract::P-31 nuclear magnetic resonance (NMR) spectroscopy was used to evaluate heterotropic cardiac allograft rejection in the rat. To enable optimal application of NMR spectroscopy for the in vivo study of myocardial metabolism in the transplanted organ, an accessory heart transplantation to groin in the rat was used. The a...


    pub_type: 杂志文章


    authors: Haug CE,Shapiro JI,Chan L,Weil R 3rd

    更新日期:1987-08-01 00:00:00

  • Basic anatomical and physiological differences between species should be considered when choosing combinations for use in models of hepatic xenotransplantation: an investigation of the guinea pig-to-rat combination.

    abstract::Published data on the guinea pig-to-rat hepatic xenotransplant model describe problems concerning poor graft reperfusion. To further investigate this phenomenon, orthotopic liver xenotransplantation between weight-matched guinea pigs and rats were performed using Kamada's technique. On reperfusion, all cases had porta...


    pub_type: 杂志文章


    authors: Delriviere LD,Havaux X,Gibbs P,Gianello PR

    更新日期:1998-07-15 00:00:00

  • Prevalence and correlates of self-reported pretransplant nonadherence with medication in heart, liver, and lung transplant candidates.

    abstract:BACKGROUND:Evidence on prevalence and correlates of pretransplant medication nonadherence (MNA) is limited. The present study explored self-reported prevalence and correlates of MNA before heart, liver, and lung transplantation. METHODS:This cross-sectional descriptive study included 174 patients: 69 lung, 33 heart, a...


    pub_type: 杂志文章


    authors: Dobbels F,Vanhaecke J,Desmyttere A,Dupont L,Nevens F,De Geest S

    更新日期:2005-06-15 00:00:00

  • The mechanism of injury in a steatotic liver graft during cold preservation.

    abstract:BACKGROUND:Fatty livers are more prone to primary nonfunction after transplantation. It is known that cell injury is strongly associated with alterations in the content and composition of membrane lipids. We assumed that plasma membrane (PM) fluidity, which is the most important property of the membrane, differed betwe...


    pub_type: 杂志文章


    authors: Fukumori T,Ohkohchi N,Tsukamoto S,Satomi S

    更新日期:1999-01-27 00:00:00

  • Immunity to Vaccine-preventable Viral Infections in Australians Being Evaluated for Liver Transplantation.

    abstract:BACKGROUND:Vaccine-preventable viral infections are associated with increased risk of morbidity and mortality in immunocompromised patients. Current guidelines recommend routine screening and vaccination of all patients before solid organ transplantation. We studied rates of immunity against vaccine-preventable viruses...


    pub_type: 杂志文章


    authors: Gardiner A,Liu K,Bonnichsen M,Joshi V,Davis RJ,Strasser SI

    更新日期:2019-11-01 00:00:00

  • Composite tissue allotransplantation: classification of clinical acute skin rejection.

    abstract:BACKGROUND:Composite tissue allotransplantation (CTA) is a recently introduced option for limb replacement and reconstruction of other nonreconstructible tissue defects. As with recipients of other allotransplants, CTA recipients can experience rejection episodes that are presumed to be mediated by immune mechanisms si...


    pub_type: 杂志文章


    authors: Cendales LC,Kirk AD,Moresi JM,Ruiz P,Kleiner DE

    更新日期:2005-12-27 00:00:00