Abstract：
:Gap junctions play a fundamental role in intercellular communication in cardiac tissue. Various types of heart disease including hypertrophy and ischemia are associated with alterations of the spatial arrangement of gap junctions. Previous studies applied two-dimensional optical and electron-microscopy to visualize gap junction arrangements. In normal cardiomyocytes, gap junctions were primarily found at cell ends, but can be found also in more central regions. In this study, we extended these approaches toward three-dimensional reconstruction of gap junction distributions based on high-resolution scanning confocal microscopy and image processing. We developed methods for quantitative characterization of gap junction distributions based on analysis of intensity profiles along the principal axes of myocytes. The analyses characterized gap junction polarization at cell ends and higher-order statistical image moments of intensity profiles. The methodology was tested in rat ventricular myocardium. Our analysis yielded novel quantitative data on gap junction distributions. In particular, the analysis demonstrated that the distributions exhibit significant variability with respect to polarization, skewness, and kurtosis. We suggest that this methodology provides a quantitative alternative to current approaches based on visual inspection, with applications in particular in characterization of engineered and diseased myocardium. Furthermore, we propose that these data provide improved input for computational modeling of cardiac conduction.
journal_name
Ann Biomed Engjournal_title
Annals of biomedical engineeringauthors
Lackey DP,Carruth ED,Lasher RA,Boenisch J,Sachse FB,Hitchcock RWdoi
10.1007/s10439-011-0369-3subject
Has Abstractpub_date
2011-11-01 00:00:00pages
2683-94issue
11eissn
0090-6964issn
1573-9686journal_volume
39pub_type
杂志文章abstract：:The pulmonary microvasculature offers a heterogeneous barrier to the motion of large solutes as they pass between blood and lymph. While this barrier has been approximated by a few discrete pathways or by statistical ensembles of many pathways, these descriptions only partly capture the structural and functional prope...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
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doi：10.1007/s10439-011-0307-4
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更新日期：2009-03-01 00:00:00
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journal_title：Annals of biomedical engineering
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更新日期：2010-05-01 00:00:00
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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